NFAT4 (NFATC3) (NM_004555) Human Untagged Clone

CAT#: SC117277

NFATC3 (untagged)-Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 (NFATC3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFATC3
• Synonyms: NF-AT4c; NFAT4; NFATX
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004555, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACTACTGCAAACTGTGGCGCCCACGACGAGCTCGACTTCAAACTCGTCTTTGGCGAGGACGGGGCGC
  CGGCGCCGCCGCCCCCGGGCTCGCGGCCTGCAGATCTTGAGCCAGATGATTGTGCATCCATTTACATCTT
  TAATGTAGATCCACCTCCATCTACTTTAACCACACCACTTTGCTTACCACATCATGGATTACCGTCTCAC
  TCTTCTGTTTTGTCACCATCGTTTCAGCTCCAAAGTCACAAAAACTATGAAGGAACTTGTGAGATTCCTG
  AATCTAAATATAGCCCATTAGGTGGTCCCAAACCCTTTGAGTGCCCAAGTATTCAAATTACATCTATCTC
  TCCTAACTGTCATCAAGAATTAGATGCACATGAAGATGACCTACAGATAAATGACCCAGAACGGGAATTT
  TTGGAAAGGCCTTCTAGAGATCATCTCTATCTTCCTCTTGAGCCATCCTACCGGGAGTCTTCTCTTAGTC
  CTAGTCCTGCCAGCAGCATCTCTTCTAGGAGTTGGTTCTCTGATGCATCTTCTTGTGAATCGCTTTCACA
  TATTTATGATGATGTGGACTCAGAGTTGAATGAAGCTGCAGCCCGATTTACCCTTGGATCCCCTCTGACT
  TCTCCTGGTGGCTCTCCAGGGGGCTGCCCTGGAGAAGAAACTTGGCATCAACAGTATGGACTTGGACACT
  CATTATCACCCAGGCAATCTCCTTGCCACTCTCCTAGATCCAGTGTCACTGATGAGAATTGGCTGAGCCC
  CAGGCCAGCCTCAGGACCCTCATCAAGGCCCACATCCCCCTGTGGGAAACGGAGGCACTCCAGTGCTGAA
  GTTTGTTATGCTGGGTCCCTTTCACCCCATCACTCACCTGTTCCTTCACCTGGTCACTCCCCCAGGGGAA
  GTGTGACAGAAGATACGTGGCTCAATGCTTCTGTCCATGGTGGGTCAGGCCTTGGCCCTGCAGTTTTTCC
  ATTTCAGTACTGTGTAGAGACTGACATCCCTCTCAAAACAAGGAAAACTTCTGAAGATCAAGCTGCCATA
  CTACCAGGAAAATTAGAGCTGTGTTCAGATGACCAAGGGAGTTTATCACCAGCCCGGGAGACTTCAATAG
  ATGATGGCCTTGGATCTCAGTATCCTTTAAAGAAAGATTCATGTGGTGATCAGTTTCTTTCAGTTCCTTC
  ACCCTTTACCTGGAGCAAACCAAAGCCTGGCCACACCCCTATATTTCGCACATCTTCATTACCTCCACTA
  GACTGGCCTTTACCAGCTCATTTTGGACAATGTGAACTGAAAATAGAAGTGCAACCTAAAACTCATCATC
  GAGCCCATTATGAAACTGAAGGTAGCCGAGGGGCAGTAAAAGCATCTACTGGGGGACATCCTGTTGTGAA
  GCTCCTGGGCTATAACGAAAAGCCAATAAATCTACAAATGTTTATTGGGACAGCAGATGATCGATATTTA
  CGACCTCATGCATTTTACCAGGTGCATCGAATCACTGGGAAGACAGTCGCTACTGCAAGCCAAGAGATAA
  TAATTGCCAGTACAAAAGTTCTGGAAATTCCACTTCTTCCTGAAAATAATATGTCAGCCAGTATTGATTG
  TGCAGGTATTTTGAAACTCCGCAATTCAGATATAGAACTTCGAAAAGGAGAAACTGATATTGGCAGAAAG
  AATACTAGAGTACGACTTGTGTTTCGTGTACACATCCCACAGCCCAGTGGAAAAGTCCTTTCTCTGCAGA
  TAGCCTCTATACCCGTTGAGTGCTCCCAGCGGTCTGCTCAAGAACTTCCTCATATTGAGAAGTACAGTAT
  CAACAGTTGTTCTGTAAATGGAGGTCATGAAATGGTTGTGACTGGATCTAATTTTCTTCCAGAATCCAAA
  ATCATTTTTCTTGAAAAAGGACAAGATGGACGACCTCAGTGGGAGGTAGAAGGGAAGATAATCAGGGAAA
  AATGTCAAGGGGCTCACATTGTCCTTGAAGTTCCTCCATATCATAACCCAGCAGTTACAGCTGCAGTGCA
  GGTGCACTTTTATCTTTGCAATGGCAAGAGGAAAAAAAGCCAGTCTCAACGTTTTACTTATACACCAGTT
  TTGATGAAGCAAGAACACAGAGAAGAGATTGATTTGTCTTCAGTTCCATCTTTGCCTGTGCCTCATCCTG
  CTCAGACCCAGAGGCCTTCCTCTGATTCAGGGTGTTCACATGACAGTGTACTGTCAGGACAGAGAAGTTT
  GATTTGCTCCATCCCACAAACATATGCATCCATGGTGACCTCATCCCATCTGCCACAGTTGCAGTGTAGA
  GATGAGAGTGTTAGTAAAGAACAGCATATGATTCCTTCTCCAATTGTACACCAGCCTTTTCAAGTCACAC
  CAACACCTCCTGTGGGGTCTTCCTATCAGCCTATGCAAACTAATGTTGTGTACAATGGACCAACTTGTCT
  TCCTATTAATGCTGCCTCTAGTCAAGAATTTGATTCAGTTTTGTTTCAGCAGGATGCAACTCTTTCTGGT
  TTAGTGAATCTTGGCTGTCAACCACTGTCATCCATACCATTTCATTCTTCAAATTCAGGCTCAACAGGAC
  ATCTCTTAGCCCATACACCTCATTCTGTGCATACCCTGCCTCATCTGCAATCAATGGGATATCATTGTTC
  AAATACAGGACAAAGATCTCTTTCTTCTCCAGTGGCTGACCAGATTACAGGTCAGCCTTCGTCTCAGTTA
  CAACCTATTACATATGGTCCTTCACATTCAGGGTCTGCTACAACAGCTTCCCCAGCAGCTTCTCATCCCT
  TGGCTAGTTCACCGCTTTCTGGGCCACCATCTCCTCAGCTTCAGCCTATGCCTTACCAATCTCCTAGCTC
  AGGAACTGCCTCATCACCGTCTCCAGCCACCAGAATGCATTCTGGACAGCACTCAACTCAAGCACAAAGT
  ACGGGCCAGGGGGGTCTTTCTGCACCTTCATCCTTAATATGTCACAGTTTGTGTGATCCAGCGTCATTTC
  CACCTGATGGGGCAACTGTGAGCATTAAACCTGAACCAGAAGATCGAGAGCCTAACTTTGCAACCATTGG
  TCTGCAGGACATCACTTTAGATGATGACCAATTTATATCTGACTTGGAACACCAGCCATCAGGTTCAGCA
  GAGAAATGGCCTAACCACAGTGTGCTCTCATGTCCAGCTCCTTTCTGGAGAATCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004555 unedited
  NTGTCGNNATATTTGTATACGACTCACTATAGGGCGGCNCGCGAATTCGCACCAGGCGGG
  CGGCTGCGGTTCCTGGTGCTGCTCGGCGCGCGGCCAGCTTTCGGAACGGAACGCTCGGCG
  TCGCGGGCCCCGCCCGGAAAGTTTGCCGTGGAGTCGCGACCTCTTGGCCCGCGCGGCCCG
  GCATGAAGCGGCGTTGAGGAGCTGCTGCCGCCGCTTGCCGCTGCCGCCGCCGCCGCCTGA
  GGAGGAGCTGCAGCACCCTGGGCCACGCCGATGACTACTGCAAACTGTGGCGCCCACGAC
  GAGCTCGACTTCAAACTCGTCTTTGGCGAGGACGGGGCGCCGGCGCCGCCGCCCCCGGGC
  TCGCGGCCTGCAGATCTTGAGCCAGATGATTGTGCATCCATTTACATCTTTAATGTAGAT
  CCACCTCCATCTACTTTAACCACACCACTTTGCTTACCACATCATGGATTACCGTCTCAC
  TCTTCTGTTTTGTCACCATCGTTTCAGCTCCAAAGTCACAAAAACTATGAAGGAACTTGT
  GAGATTCCTGAATCTAAATATAGCCCATTAGGTGGTCCCAAACCCTTTGAGTGCCCAAGT
  ATTCAAATTACATCTATCTCTCCTAACTGTCATCAAGAATTAGATGCACATGAAGATGAC
  CTACAGATAAATGACCCAGAACGGGAATTTTTGGAAAGGCCTTCTAGAGATCATCTCTAT
  CTTCCTCTTGAGCCATCCTACCGGGAGTCTTCTCTTAGTCCTAGTCCTGCCAGCAGCATC
  TCTTCTAGGAGTTGGNTCTCTGATGCATCTTCTTGTGAATCGCTTTCACATATTTATGAT
  GATGTGGACTCAGAGTTGAATGAAGCTGCAGCCCGATTTACCCTTGATCCCCTCTGACTT
  CTCTGGGGNCTCCTCGGGGGCTGCCCTGGAGAGAAACTTGCATCACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004555 unedited
  CTATGAACCGCGGCACGCAATCTAGTATCGAGTNCTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTGGGCTCTAAAAGGCTTATAAAAAGGTCCTTTTTTA
  AAAATTATCCTAAAAACCAAAGGGGCCCTTAAATGACCCTTAAGGAGTTCAAATTACCAA
  TTCTTTCTTAATAACCAGGGCACCTGGAAAAGCAGCACCCCGGAAACAAAAAAACCTGGT
  GGATTTACACTCAAAGGACCCCTCATATAAAAGGATCCCAATTAAATATAAAAAAGAAAG
  TTTATTTCCCCAAAGCTTAAAAAGCCTATGCTGGTCCTGGTTTTTTGCCAAAATTACCCA
  AGGGCCGAGGGGGGGGAATGGTTCTTTTCCCCACAAATGGGGGGGTCCCCTCCTGGGCCC
  CCAACTTAAGGGTTGAAAATCTGAAAACCTAAGCGCAACGTAATAAACACTGTCGGAAAA
  TTTGGTGGGGGGACTAAAGGCGCAGTACAACCCCTTTTAAGCCCCCTACATTTTTTCTTT
  TTTATTGGTCATATTACTCTCTTCCGCGATCCTCTTCTCTCTTCCCATTTCCTAATCACC
  CACATTTACCACCACCCCTTCTACCAATAATCATTCCTCACACCTCCTTCTCACATCGTC
  CATCTTCTATTTGGCTAACCACCTGTTGTCTCGACCTCATACTTCCGTTTTACCTTACCA
  ACTTCCTCCTATTCTATCTTCTGGCCAACACTTATCTCACCTACTGCGCCACCCCCCCCA
  GCCCCATACCTAACCATTTATATATAGACAACCTCTCCATCTTTCTATCTTATCGCCTGC
  TTCACTTGCCACCCCGACCATATGTTTTCTACTCTATTCCTCCCGTGTAGTTACACTCCC
  GCCAAGTCTTCTCTCTATAACCCGCCTCGTTCATCCTATTCCACTTCTAACACCAGTACC
  ACCCCTCTTTTCATTCACATTTATGTCTTCTGTATTCCACACCACGCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004555
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004555.2, NP_004546.1
• RefSeq Size: 4005 bp
• RefSeq ORF: 3207 bp
• Locus ID: 4775
• Cytogenetics: 16q22.1
• Domains: RHD, IPT
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• Gene Summary: 'The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2010]'
  Transcript Variant: This variant (2) contains an additional segment in the coding region, which causes a frameshift, compared to variant 1. The resulting isoform (2) contains a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.