NDUFB4 (NM_004547) Human Untagged Clone

CAT#: SC117304

NDUFB4 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa (NDUFB4), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFB4
• Synonyms: B15; CI-B15
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117304 sequence for NM_004547 edited (data generated by NextGen Sequencing)
  ATGTCGTTCCCAAAGTATAAGCCGTCGAGCCTGCGCACTCTGCCTGAGACCCTCGACCCA
  GCCGAATACAACATATCTCCGGAAACCCGGCGGGCGCAAGCCGAGCGGTTGGCCATAAGA
  GCCCAGCTGAAACGAGAGTACCTGCTTCAGTACAACGATCCCAACCGCCGAGGGCTCATC
  GAAAATCCTGCCTTGCTTCGTTGGGCCTATGCAAGAACAATAAATGTCTATCCTAATTTC
  AGACCCACTCCTAAAAACTCACTCATGGGAGCTCTGTGTGGATTTGGGCCCCTCATCTTC
  ATTTATTATATTATCAAAACTGAGAGGGATAGGAAAGAAAAACTTATCCAGGAAGGAAAA
  TTGGATCGAACATTTCACCTCTCATATTAA
  
  Clone variation with respect to NM_004547.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004547 unedited
  GGCGGCCGCGAATTCGGCACGAGAGATGTCGTTCCCAAAGTATAAGCCGTCGAGCCTGCG
  CACTCTGCCTGAGACCCTCGACCCAGCCGAATACAACATATCTCCGGAAACCCGGCGGGC
  GCAAGCCGAGCGGTTGGCCATAAGAGCCCAGCTGAAACGAGAGTACCTGCTTCAGTACAA
  CGATCCCAACCGCCGAGGGCTCATCGAAAATCCTGCCTTGCTTCGTTGGGCCTATGCAAG
  AACAATAAATGTCTATCCTAATTTCAGACCCACTCCTAAAAACTCACTCATGGGAGCTCT
  GTGTGGATTTGGGCCCCTCATCTTCATTTATTATATTATCAAAACTGAGAGGGATAGGAA
  AGAAAAACTTATCCAGGAAGGAAAATTGGATCGAACATTTCACCTCTCATATTAAGTCTG
  GCAATGATGACTATATGTATTCCTGCCTAAATAAATCATCTATTAATCATTAAAAGAAAA
  AAAAAAAAACTCGACTTTATATTGCGGCCGGGGTAATATCTTTTTCCTGAACAAATCCCG
  GGGTGGGATCCCTTGGACCCCTCCCCAGGGCCTTCCTGGCCCTGGAAGTTGCCCCTCAGG
  GCCCCCAGCCTTGGCCTAATAAATAAGTGGATATTGTGTGNGNNNCNNNNNNNAAAAANN
  AAAAAANNNNGAGGAGAGNCNNAGAGCAGGGGGAGAAAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004547 unedited
  CCAATTTGNCCGGGCCGCATTCTACACNCGGTTTTTTTTTTTTTTTTTTAATGATTAATA
  GATGATTTATTTAGGCAGGAATACATATAGTCATCATTGCCAGACTTAATATGAGAGGTG
  AAATGTTCGATCCAATTTTCCTTCCTGGATAAGTTTTTCTTTCCTATCCCTCTCAGTTTT
  GATAATATAATAAATGAAGATGAGGGGCCCAAATCCACACAGAGCTCCCATGAGTGAGTT
  TTTAGGAGTGGGTCTGAAATTAGGATAGACATTTATTGTTCTTGCATAGGCCCAACGAAG
  CAAGGCAGGATTTTCGATGAGCCCTCGGCGGTTGGGATCGTTGTACTGAAGCAGGTACTC
  TCGTTTCAGCTGGGCTCTTATGGCCAACCGCTCGGCTTGCGCCCGCCGGGTTTCCGGAGA
  TATGTTGTATTCGGCTGGGTCGAGGGTCTCAGGCAGAGTGCGCAGGCTCGACGGCTTATA
  CTTTGGGAACGACATCTCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACA
  AAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTA
  CCGCCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGATTT
  TGGTGCCAAAACAAACTCCCATTGACGTCACATGGGGTGGAGACTTGGAAATCCCTGTGA
  GTCACACCGCTATCCACGCCCATTGGTGTACTGCCAACACCGCATCACCATGGTAATAGC
  GATGACTAATACGTACATGTACTGCCAAGTAGGAAAGTCCCGTANGGCCATGTACTGGGC
  ATCATGCTCACGCGGCCATATACCGTCATTGACGTCAATAGGGGGCCGAACTTGGCTATG
  ATCACCTTGATGTCTGCCAGANNGGCAGTTTACCGTCAAACTCACCCTATGCCGCCAATG
  GAAGCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004547
• Insert Size: 500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004547.4, NP_004538.2
• RefSeq Size: 541 bp
• RefSeq ORF: 390 bp
• Locus ID: 4710
• Cytogenetics: 3q13.33
• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone. [provided by RefSeq, Dec 2009]'
  Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.