NDUFA1 (NM_004541) Human Untagged Clone

CAT#: SC117328

NDUFA1 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa (NDUFA1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFA1
• Synonyms: CI-MWFE; MC1DN12; MWFE; ZNF183
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117328 sequence for NM_004541 edited (data generated by NextGen Sequencing)
  ATGTGGTTCGAGATTCTCCCCGGACTCTCCGTCATGGGCGTGTGCTTGTTGATTCCAGGA
  CTGGCTACTGCGTACATCCACAGGTTCACTAACGGGGGCAAGGAAAAAAGGGTTGCTCAT
  TTTGGGTATCACTGGAGTCTGATGGAAAGAGATAGGCGCATCTCTGGAGTTGATCGTTAC
  TATGTGTCAAAGGGTTTGGAGAACATTGATTAA
  
  Clone variation with respect to NM_004541.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004541 unedited
  TAGTTGCGTAGACGCACTATAGGCGGCCGCGCATGNCGCGCGGGGGCGAAGCCAGGTCAC
  CTTTCAGGACCCAGAATAGGGTTTTGGCCTAGGTAACGGGGCAGAGATGTGGTTCGAGAT
  TCTCCCCGGACTCTCCGTCATGGGCGTGTGCTTGTTGATTCCAGGACTGGCTACTGCGTA
  CATCCACAGGTTCACTAACGGGGGCAAGGAAAAAAGGGTTGCTCATTTTGGGTATCACTG
  GAGTCTGATGGAAAGAGATAGGCGCATCTCTGGAGTTGATCGTTACTATGTGTCAAAGGG
  TTACGAGAACATTGATTAAGGAAGCATTTTCCTGATTGATGAAAAAAATAACTCAGTTAT
  GGCCATCTACCCCTGCTAGAAGGTTACAGTGTATTATGTAGCATGCAATGTGTTATGTAG
  TGCTTAATAAAAATAAAATGAACAAAAAAAAAAAAAAAAAAAAAAAAACAACTCGACTCT
  AGATTGCGGCCGCGGCCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACC
  CCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCC
  TAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATTATGGGGTG
  GAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAAACAACCTGTAGGGCCTGCGGGGT
  CTATTGGGAACCAAGCTGGAGTGCAGCGGCCCAATCCTTGGCTCACTGCAATCTCCGCCT
  CCCTGAGTTCAAGCGGATTCTCTTGCCTCAACCTCCCCGAGTTTGTTTGGATTCCACGGC
  ATGCATGACCANGCTTCAACTAATTTTTGCTTTTCTTGAACAGACGGGGTTTACCATATT
  GGCACGGTGGGTCTCAAACTCTAATTCCAGGGATCTACCCCCTCTGCCCCCAAATGGTGG
  GATTCACGCGTGAACCCTGTCCCTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004541 unedited
  NTTTTACTCTGNACCGCGGCCCGCATTTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTGTTAATTTTAATAAAGCACTACATAACACATTGCATGCTACATAATACA
  CTGAAACCTTCTACCAGGGGTAGATGGCCATAACTGAGTTATTTTTTTCATCAATCAGGA
  AAATGCTTCCTTAATCAAGGTTCTCCAAACCCTTTGACACATATTAACGACCAACTCCAA
  AGATGCGCCTATCTCTTTCCATCATACTCCAGGGATACCCAAAATGAGCAACCCTTTTTT
  CCTTGCCCCCGTTAGTGAACCTGTGGATGTCCGCACTATCCAGCCCTGGAATCAACAACC
  ACACGCCCATGACGGAAAGCCCGGGGAGAATCTCGAACCACATCTTTGCCCCGTTACCTA
  GGCCAAAACCCTACTCTTGGGCCCTTGAAAGGGGACCTGCTTTCGCCCCTTGGGCCGAAC
  TCCCGCCGCCCTCTCGTGACTCCCATTCACAAACCCCGCCGCGCCATTACACCACCCCTG
  CTTATATAAACCCCCCCCCCCTACCCCCCCCCCGCCCCTTTTGCTCCACCGGCCGGCCGT
  TTCTCCCCCCCTTTTTGGCCATTCCCGCCCATCCCTGGCCCCCAACCAACCCGCCCATGG
  ACCCCTCGCGGGGGGGCGCACGGGACAACCCTGCGGACAACACCCCCTCCCCACCACCAT
  TCGGTGCGCTCGCCACCACCCTCGCCCGCTCCGTATACCCCGGCCTACTCCTTCCCCCGT
  TCCGCCCCCCCCGAACCCCCCTAGAGCCCCGCCCCCGGCATAAATGCCCCGGCGGCCCTC
  TACCCGGCCTTCCGCCCCTGGGCGCCCTCCCGGTTTACCACCCCCCACGTCTGCCCCGCC
  GCCCTTCCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004541
• Insert Size: 460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004541.2, NP_004532.1
• RefSeq Size: 479 bp
• RefSeq ORF: 213 bp
• Locus ID: 4694
• Cytogenetics: Xq24
• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq, Jul 2008]'