Glypican 3 (GPC3) (NM_004484) Human Untagged Clone

CAT#: SC117333

GPC3 (untagged)-Human glypican 3 (GPC3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPC3
• Synonyms: DGSX; GTR2-2; MXR7; OCI-5; SDYS; SGB; SGBS; SGBS1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117333 sequence for NM_004484 edited (data generated by NextGen Sequencing)
  ATGGCCGGGACCGTGCGCACCGCGTGCTTGGTGGTGGCGATGCTGCTCAGCTTGGACTTC
  CCGGGACAGGCGCAGCCCCCGCCGCCGCCGCCGGACGCCACCTGTCACCAAGTCCGCTCC
  TTCTTCCAGAGACTGCAGCCCGGACTCAAGTGGGTGCCAGAAACTCCCGTGCCAGGATCA
  GATTTGCAAGTATGTCTCCCTAAGGGCCCAACATGCTGCTCAAGAAAGATGGAAGAAAAA
  TACCAACTAACAGCACGATTGAACATGGAACAGCTGCTTCAGTCTGCAAGTATGGAGCTC
  AAGTTCTTAATTATTCAGAATGCTGCGGTTTTCCAAGAGGCCTTTGAAATTGTTGTTCGC
  CATGCCAAGAACTACACCAATGCCATGTTCAAGAACAACTACCCAAGCCTGACTCCACAA
  GCTTTTGAGTTTGTGGGTGAATTTTTCACAGATGTGTCTCTCTACATCTTGGGTTCTGAC
  ATCAATGTAGATGACATGGTCAATGAATTGTTTGACAGCCTGTTTCCAGTCATCTATACC
  CAGCTAATGAACCCAGGCCTGCCTGATTCAGCCTTGGACATCAATGAGTGCCTCCGAGGA
  GCAAGACGTGACCTGAAAGTATTTGGGAATTTCCCCAAGCTTATTATGACCCAGGTTTCC
  AAGTCACTGCAAGTCACTAGGATCTTCCTTCAGGCTCTGAATCTTGGAATTGAAGTGATC
  AACACAACTGATCACCTGAAGTTCAGTAAGGACTGTGGCCGAATGCTCACCAGAATGTGG
  TACTGCTCTTACTGCCAGGGACTGATGATGGTTAAACCCTGTGGCGGTTACTGCAATGTG
  GTCATGCAAGGCTGTATGGCAGGTGTGGTGGAGATTGACAAGTACTGGAGAGAATACATT
  CTGTCCCTTGAAGAACTTGTGAATGGCATGTACAGAATCTATGACATGGAGAACGTACTG
  CTTGGTCTCTTTTCAACAATCCATGATTCTATCCAGTATGTCCAGAAGAATGCAGGAAAG
  CTGACCACCACTATTGGCAAGTTATGTGCCCATTCTCAACAACGCCAATATAGATCTGCT
  TATTATCCTGAAGATCTCTTTATTGACAAGAAAGTATTAAAAGTTGCTCATGTAGAACAT
  GAAGAAACCTTATCCAGCCGAAGAAGGGAACTAATTCAGAAGTTGAAGTCTTTCATCAGC
  TTCTATAGTGCTTTGCCTGGCTACATCTGCAGCCATAGCCCTGTGGCGGAAAACGACACC
  CTTTGCTGGAATGGACAAGAACTCGTGGAGAGATACAGCCAAAAGGCAGCAAGGAATGGA
  ATGAAAAACCAGTTCAATCTCCATGAGCTGAAAATGAAGGGCCCTGAGCCAGTGGTCAGT
  CAAATTATTGACAAACTGAAGCACATTAACCAGCTCCTGAGAACCATGTCTATGCCCAAA
  GGTAGAGTTCTGGATAAAAACCTGGATGAGGAAGGGTTTGAAAGTGGAGACTGCGGTGAT
  GATGAAGATGAGTGCATTGGAGGCTCTGGTGATGGAATGATAAAAGTGAAGAATCAGCTC
  CGCTTCCTTGCAGAACTGGCCTATGATCTGGATGTGGATGATGCGCCTGGAAACAGTCAG
  CAGGCAACTCCGAAGGACAACGAGATAAGCACCTTTCACAACCTCGGGAACGTTCATTCC
  CCGCTGAAGCTTCTCACCAGCATGGCCATCTCGGTGGTGTGCTTCTTCTTCCTGGTGCAC
  TGA
  
  Clone variation with respect to NM_004484.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004484 unedited
  GAGATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCAGCGCCCAGGTAGCT
  GCGAGGAAACTTTTGCAGCGGCTGGGTAGCAGCACGTCTCTTGCTCCTCAGGGCCACTGC
  CAGGCTTGCCGAGTCCTGGGACTGCTCTCGCTCCGGCTGCCACTCTCCCGCGCTCTCCTA
  GCTCCCTGCGAAGCAGGATGGCCGGGACCGTGCGCACCGCGTGCTTGGTGGTGGCGATGC
  TGCTCAGCTTGGACTTCCCGGGACAGGCGCAGCCCCCGCCGCCGCCGCCGGACGCCACCT
  GTCACCAAGTCCGCTCCTTCTTCCAGAGACTGCAGCCCGGACTCAAGTGGGTGCCAGAAA
  CTCCCGTGCCAGGATCAGATTTGCAAGTATGTCTCCCTAAGGGCCCAACATGCTGCTCAA
  GAAAGATGGAAGAAAAATACCAACTAACAGCACGATTGAACATGGAACAGCTGCTTCAGT
  CTGCAAGTATGGAGCTCAAGTTCTTAATTATTCAGAATGCTGCGGTTTTCCAAGAGGCCT
  TTGAAATTGTTGTTCGCCATGCCAAGAACTACACCAATGCCATGTTCAAGAACAACTACC
  CAAGCCTGACTCCACAAGCTTTTGAGTTTGTGGGTGAATTTTTCACAGATGTGTCTCTCT
  ACCATCTGGGTTCTGACATCAATGTAGATGACATGCTCAATGAATTGTTTGACAGCCTGT
  TTCCAGTCATCTATACCCAGCTAATGAACCCCAGCCCTGCCTGATTCACCCTTGGACATC
  AATGAGTGCCTCCCGAGGAGCAAGACGTGACCCTGAAAGTATTTGGGAATTTCCCCAGCT
  TATTATGACCCAGNTCTCCAAGCCCTGGAAGTCACTAGACCTTCCTTAGGCTCTGATCCT
  GGATGGAGGGTCACCCAACTGACCCCTGAGTTCATAGGACTGGGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004484 unedited
  GCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGAGCAATTTATTTTTTTTCA
  AAGAAATCCATGCAAAGAGAGAACGATAGAAAGGGAGGGAGTGAGAGAGAAAGACATGGT
  AACCATGTTCTAGCAGCCAAACATAGGAGAATAATTTGGCACAACTTGATGGTTTTTTTT
  CTTTCTTTGCAAAAGGACAATCTATATGCTACCACTAAAATGTATCTTCCTCCAAAAGAT
  ACCATTTGATTTTCGAAAACATAACACATGGTTAGTCCTCTACTTCATGGCTGGAGGAGG
  TATACAGGATAACAAAAAAAAAAAAATAGAAAAAAATAAGAAAGTGGTTCCCTTTATCGA
  GGAAGACCACAGGGTGCTGTAGGGCAGCACATGTGCTGGGCACCAGGCAGTCAGTGCACC
  AGGAAGAAGAAGCACACCACCGAGATGGCCATGCTGGTGAGAAGCTTCAGCGGGGAATGA
  ACGTTCCCGAGGTTGTGAAAGGTGCTTATCTCGTTGTCCTTCGGAGTTGCCTGCTGACTG
  TTTCCAGGCGCATCATCCACATCCAGATCATAGGCCAGTTCTGCAAGGAAGCGGAGCTGA
  TTCTTCACTTTTATCATTCCATCACCAGAGCCTNCAATGCACTCATCTTCATCATCACCG
  CAGTCTCCACTTTCAAACCCTTCCTCATCCAGGTTTTTATCCAGAACTCTACCTTTTGGC
  ATAGACATGGCTCTCAGGAGCTGTNTATGTGCCTCAGTTTGTCAATAATTGACTGACACT
  GCCTCAGGCCCCTTCATTTCAGCTCATGGAGATGGAACGGGTTTTCATCCAATCCTTGCT
  GCCTTTGGCTGGACTCTCCCGAGTTTCTGTCCTTTCAGCCAAGGTGGCGTTTCCGCCACA
  GGCTATGCTGCGAAGTACCCCGCAAGACTTTAAAGCTGGGAAGACTCACCTCTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004484
• Insert Size: 2550 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004484.2, NP_004475.1
• RefSeq Size: 2382 bp
• RefSeq ORF: 1743 bp
• Locus ID: 2719
• Cytogenetics: Xq26.2
• Domains: Glypican
• Protein Families: Druggable Genome
• Gene Summary: 'Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.