ETFDH (NM_004453) Human Untagged Clone

CAT#: SC117365

ETFDH (untagged)-Human electron-transferring-flavoprotein dehydrogenase (ETFDH), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ETFDH
• Synonyms: ETFQO; MADD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004453, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGGTGCCGCTAGCCAAGCTGTCCTGCCTGGCATATCAGTGCTTTCATGCCTTAAAAATTAAGAAAA
  ATTATCTACCTCTATGTGCTACAAGATGGTCTTCAACTTCTACTGTGCCTCGAATTACTACCCATTATAC
  TATTTATCCCCGGGATAAGGACAAGAGATGGGAAGGAGTGAACATGGAAAGGTTTGCAGAAGAAGCAGAT
  GTTGTAATAGTTGGTGCAGGCCCTGCAGGGCTCTCTGCAGCTGTTCGTCTAAAACAGTTGGCTGTGGCAC
  ATGAAAAGGACATCCGTGTGTGTCTAGTGGAGAAAGCTGCCCAGATAGGAGCTCATACTCTCTCAGGGGC
  TTGCCTTGATCCAGGTGCTTTTAAAGAACTCTTCCCAGACTGGAAAGAGAAGGGGGCTCCACTTAACACT
  CCTGTAACAGAAGACAGATTTGGAATTTTAACAGAGAAATACAGAATTCCTGTGCCAATTCTTCCAGGGC
  TTCCAATGAATAATCATGGCAATTACATTGTACGCTTGGGACATTTAGTGAGCTGGATGGGCGAACAAGC
  AGAAGCCCTTGGTGTTGAAGTATACCCTGGTTATGCAGCTGCTGAGGTCCTTTTTCATGATGATGGTAGT
  GTAAAAGGAATTGCCACTAACGATGTAGGGATACAAAAGGATGGTGCACCAAAGGCAACATTTGAGAGAG
  GACTGGAACTACATGCTAAAGTCACAATTTTTGCAGAAGGTTGCCATGGACATCTAGCCAAGCAACTATA
  TAAGAAGTTTGATTTGAGAGCAAATTGTGAACCTCAAACCTACGGGATTGGACTGAAGGAGTTATGGGTT
  ATTGATGAAAAGAACTGGAAACCTGGGAGAGTAGATCACACTGTTGGTTGGCCCTTGGACAGACATACCT
  ATGGAGGATCTTTCCTCTATCATTTGAATGAAGGTGAACCCCTAGTAGCTCTTGGTCTTGTGGTTGGTCT
  AGACTATCAGAATCCATACCTGAGTCCATTTAGAGAGTTCCAAAGGTGGAAACACCATCCTAGCATTCGG
  CCAACCTTGGAAGGTGGAAAAAGGATTGCATACGGAGCCAGAGCTCTCAATGAAGGTGGCTTTCAGTCTA
  TACCAAAACTCACCTTTCCTGGTGGTTTACTAATTGGTTGTAGTCCTGGTTTTATGAATGTTCCCAAGAT
  CAAAGGTACTCACACAGCAATGAAAAGTGGAATTTTAGCAGCAGAATCTATTTTTAATCAACTAACTAGT
  GAAAATCTCCAATCAAAGACAATAGGACTCCATGTAACTGAATATGAGGACAATTTGAAGAACTCATGGG
  TATGGAAAGAGCTATATTCTGTTAGAAATATAAGACCGTCCTGCCACGGAGTACTGGGTGTATATGGAGG
  GATGATTTACACTGGAATCTTTTACTGGATATTGAGAGGAATGGAGCCGTGGACTCTGAAACATAAAGGT
  TCTGACTTTGAACGGCTCAAGCCAGCCAAGGATTGCACACCTATTGAGTATCCAAAACCCGATGGACAGA
  TCAGTTTTGACCTCTTGTCATCTGTGGCTCTGAGTGGTACTAATCATGAACATGACCAGCCGGCACACTT
  AACCTTAAGGGATGACAGTATACCTGTAAATAGAAATCTGTCGATATATGATGGGCCCGAGCAGCGATTC
  TGTCCTGCAGGAGTTTATGAATTTGTACCTGTGGAACAAGGTGATGGATTTCGGTTACAGATAAATGCTC
  AGAACTGTGTACATTGTAAAACATGTGATATTAAAGATCCAAGTCAGAATATTAACTGGGTGGTACCTGA
  AGGTGGAGGAGGACCTGCTTACAATGGAATGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004453 unedited
  GCACGAGGGCAGCCTGGGTGACAGAGTGAGACTCTTGTCTCNNNNNNNNNNNNNNNNNNN
  NNNNAAAAAAAAGATGATGATAAAGAAGATGATACTGCCTTAAAAACCTGACCATAAAAC
  AAAGTTTGTGCTTAAAGCCATTGACCTAACACGGTGATTATACTACTCCATATTACTCTC
  AAAGTGTTCAGCTTTCATGACTAATCCATAGTTAAGCCTGACATGAGCTAAATTGAATAA
  ATTTGCTTATTAGTTTTAAAATATTTAAGATTTGAAAACATTGCTTTATGAGAAAAAGCT
  ATTCAAAGTGTTCACCTAGGAAAGATTATAGTATATTTCACTATACTCATTGAGTATAGT
  CATTCACTTATATTTTTTCAGTCTACTGAGGAAAACTAATTTTAAGGAAGATAATAATTT
  TCGTAATTTTTGTGCACATATCAGTGCTTTCATGCCTTAAAAATTAAGAAAAATTATCTA
  CCTCTATGTGTATAAGATGGTCTTCAACTTCTACTGTGCCTCGAATTACTACCCATTATA
  CTATTTATCCCCGGATAAGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004453
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004453.1, NP_004444.1
• RefSeq Size: 2396 bp
• RefSeq ORF: 1854 bp
• Locus ID: 2110
• Cytogenetics: 4q32.1
• Domains: ETFD
• Gene Summary: 'This gene encodes a component of the electron-transfer system in mitochondria and is essential for electron transfer from a number of mitochondrial flavin-containing dehydrogenases to the main respiratory chain. Mutations in this gene are associated with glutaric acidemia. Alternatively spliced transcript variants that encode distinct isoforms have been observed. [provided by RefSeq, Aug 2013]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1).