Alpha Dystroglycan (DAG1) (NM_004393) Human Untagged Clone

CAT#: SC117393

DAG1 (untagged)-Human dystroglycan 1 (dystrophin-associated glycoprotein 1) (DAG1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DAG1
• Synonyms: 156DAG; A3a; AGRNR; DAG; LGMDR16; MDDGA9; MDDGC7; MDDGC9
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004393, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGATGTCTGTGGGCCTCTCGCTGCTGCTGCCCCTCTGGGGGAGGACCTTTCTCCTCCTGCTCTCTG
  TGGTTATGGCTCAGTCCCACTGGCCCAGTGAACCCTCAGAGGCTGTCAGGGACTGGGAAAACCAGCTTGA
  GGCATCCATGCACTCAGTGCTCTCAGACCTCCACGAGGCTGTTCCCACAGTGGTTGGCATTCCTGATGGC
  ACGGCTGTCGTCGGGCGCTCATTTCGAGTGACCATTCCAACAGATTTGATTGCCTCCAGTGGAGATATCA
  TCAAGGTATCAGCGGCAGGGAAGGAGGCTTTGCCATCTTGGCTGCACTGGGACTCACAGAGCCACACCCT
  GGAGGGCCTCCCCCTTGACACTGATAAGGGTGTGCATTACATTTCAGTGAGCGCTACACGGCTGGGGGCC
  AACGGGAGCCACATCCCCCAGACCTCCAGTGTGTTCTCCATCGAGGTCTACCCTGAAGACCACAGTGAGC
  TGCAGTCGGTGAGGACAGCCTCCCCAGACCCTGGTGAGGTGGTATCATCTGCCTGTGCTGCGGATGAACC
  TGTGACTGTTTTGACGGTGATTTTGGATGCCGACCTCACCAAGATGACCCCAAAGCAAAGGATTGACCTC
  CTGCACAGGATGCGGAGCTTCTCAGAAGTAGAGCTTCACAACATGAAATTAGTGCCGGTGGTGAATAACA
  GACTATTTGACATGTCGGCCTTCATGGCTGGCCCGGGAAATGCAAAAAAGGTGGTGGAGAATGGGGCCCT
  TCTCTCCTGGAAGCTGGGCTGCTCCCTGAACCAGAACAGTGTGCCTGACATTCATGGTGTAGAGGCCCCT
  GCCAGGGAGGGCGCAATGTCTGCTCAGCTTGGCTACCCTGTGGTGGGTTGGCACATCGCCAATAAGAAGC
  CCCCTCTTCCCAAACGCGTCCGGAGGCAGATCCATGCTACACCCACACCTGTCACTGCCATTGGGCCCCC
  AACCACGGCTATCCAGGAGCCCCCATCCAGGATCGTGCCAACCCCCACATCTCCAGCCATTGCTCCTCCA
  ACAGAGACCATGGCTCCTCCAGTCAGGGATCCTGTTCCTGGGAAACCCACGGTCACCATCCGGACTCGAG
  GCGCCATTATTCAAACCCCAACCCTAGGCCCCATCCAGCCTACTCGGGTGTCAGAAGCTGGCACCACAGT
  TCCTGGCCAGATTCGCCCAACGATGACCATTCCTGGCTATGTGGAGCCTACTGCAGTTGCTACCCCTCCC
  ACAACCACCACCAAGAAGCCACGAGTATCCACACCAAAACCAGCAACGCCTTCAACTGACTCCACCACCA
  CCACGACTCGCAGGCCAACCAAGAAACCACGGACACCCCGGCCAGTGCCCCGGGTCACCACCAAAGTTTC
  CATCACCAGATTGGAAACTGCCTCACCGCCTACTCGTATTCGCACCACCACCAGTGGAGTGCCCCGTGGC
  GGAGAACCCAACCAGCGCCCAGAGCTCAAGAACCATATTGACAGGGTAGATGCCTGGGTTGGCACCTACT
  TTGAGGTGAAGATCCCGTCAGACACTTTCTATGACCATGAGGACACCACCACTGACAAGCTGAAGCTGAC
  CCTGAAACTGCGGGAGCAGCAGCTGGTGGGCGAGAAGTCCTGGGTACAGTTCAACAGCAACAGCCAGCTC
  ATGTATGGCCTTCCCGACAGCAGCCACGTGGGCAAACACGAGTATTTCATGCATGCCACAGACAAGGGGG
  GCCTGTCGGCTGTGGATGCCTTCGAGATCCACGTCCACAGGCGCCCCCAAGGGGATAGGGCTCCTGCAAG
  GTTCAAGGCCAAGTTTGTGGGTGACCCGGCACTGGTGTTGAATGACATCCACAAGAAGATTGCCTTGGTA
  AAGAAACTGGCCTTCGCCTTTGGAGACCGAAACTGTAGCACCATCACCCTGCAGAATATCACCCGGGGCT
  CCATCGTGGTGGAATGGACCAACAACACACTGCCCTTGGAGCCCTGCCCCAAGGAGCAGATCGCTGGGCT
  GAGCCGCCGGATCGCTGAGGATGATGGAAAACCTCGGCCTGCCTTCTCCAACGCCCTAGAGCCTGACTTT
  AAGGCCACAAGCATCACTGTGACGGGCTCTGGCAGTTGTCGGCACCTACAGTTTATCCCTGTGGTACCAC
  CCAGGAGAGTGCCCTCAGAGGCGCCGCCCACAGAAGTGCCTGACAGGGACCCTGAGAAGAGCAGTGAGGA
  TGATGTCTACCTGCACACAGTCATTCCGGCCGTGGTGGTCGCAGCCATCCTGCTCATTGCTGGCATCATT
  GCCATGATCTGCTACCGCAAGAAGCGGAAGGGCAAGCTTACCCTTGAGGACCAGGCCACCTTCATCAAGA
  AGGGGGTGCCTATCATCTTTGCAGACGAACTGGACGACTCCAAGCCCCCACCCTCCTCCAGCATGCCACT
  CATTCTGCAGGAGGAGAAGGCTCCCCTACCCCCTCCTGAGTACCCCAACCAGAGTGTGCCCGAGACCACT
  CCTCTGAACCAGGACACCATGGGAGAGTACACGCCCCTGCGGGATGAGGATCCCAATGCGCCTCCCTACC
  AGCCCCCACCGCCCTTCACAGCACCCATGGAGGGCAAGGGCTCCCGTCCCAAGAACATGACCCCATACCG
  GTCACCTCCTCCCTATGTCCCACCTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004393 unedited
  CTTTGGATTTTGTAACACGACTCACTATGGGCGGCACGCGATTCGGCACGAGGGCGGCGC
  TCGCGCCTCTTATGCTTGGCGGTGGCGGCGGCGGCAGCTTCGCGCCGAATCCCCGGGGAG
  CGGCGGTGGCGGCGTCCTGGGGCCAGGAGGAGCGAACACCTGCCGCATTCCTCCCGCCGG
  CGCTGGGCTCTGTGTGCTCCGGGATGGAGCAGGTGTGCAGAGGGTGAGAACCCAGCTCTG
  GGACCAAGTCACTTGCTTCCTTACTTAGCAAGACTATCGACTTGAGCAAACTTGGACCTG
  GGATGAGGATGTCTGTGGGCCTCTCGCTGCTGCTGCCCCTCTGGGGGAGGACCTTTCTCC
  TCCTGCTCTCTGTGGTTATGGCTCAGTCCCACTGGCCCAGTGAACCCTCAGAGGCTGTCA
  GGGACTGGGAAAACCAGCTTGAGGCATCCATGCACTCAGTGCTCTCAGACCTCCACGAGG
  CTGTTCCCACAGTGGTTGGCATTCCTGATGGCACGGCTGTCGTCGGGCGCTCATTTCGAG
  TGACCATTCCAACAGATTTGATTGCCTCCAGTGGAGATATCATCAAGGTATCAGCGGCAG
  GGAAGGAGGCTTTGCCATCTTGGCTGCACTGGGACTCACAGAGCCACACCCTGGAGGGCC
  TCCCCCTTGACACTGTAAGGGTGTGCATTACATTTCAGTGAGCGCTACACGGCTGGNGGC
  CAACGGGAGCCACATCCNCCAGACCTCCAGTGTGTTCTCAATCGAGGTCTACCCTGGAGA
  CACAGTGAGCTGCAGTCGGTGGAGGACAGCCTCCCAGACCTGGGGGAGGTGTATCATCTG
  CCTGTGCTGGGGATGACCTGTGACTGTTTTGACGGTGGATTTGGAATGCCGCCCTTACAA
  GATGACCCCAAAACAAAGAATGACCTCCTGCACAGGATGCGGACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004393 unedited
  TTTAGCTATGGCACGCGGCACGCAATCTAGTATCGAGTTCTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTCCCAATTAAAATCTTTTTTTTAATAAAACAATTGGAGGCTGTATAAA
  AACTTTAGAAAAAAATTACCTTTGAAAGCTAACAAATTTTTATTATGAACTATGGCTGGC
  ACTCCTCCCCAGCCGCCAAACCTTTTCCAGTATTTCAGGCCCAAGGCCTGGGACTGGAGG
  GCCAAAAAAAAGGGATGGGGGGGGTCGGGGCAGGACTGGGGACTGTTAGGACAAAGCGGC
  CAAGGTCAAGGGAGGAAAGAGCATCCCATGACATAAAAATATTGCTTCTTCACCAAAATG
  TCCCCCACTGAGGGTTTCAAAAAACCGGGATATTCCTTTTAGGAGGAAAAAAAAAACCAA
  GGCCCAAAAAAGGGGTAAAACAAAATAGGTTGATGGGGGGGAAACCTTTGACCTCCCAGG
  GGCCCCCACAAGCAGTTGGCCCACCCTGGAGCACCAAAGTTGGGGGCCACAGCCACCATT
  GCCTTTTGGAGTCCCTTTTTCATGGAAATGAAAACCCCTTGGCCGGGGCCCACTAGTGTA
  CATGGGCTGGAAGGGGGGATGCCCACGGCCCAAGAAACCCCCCCAGCTTCCACCCCCTGG
  TATTAGTGGGTGATCAAACCCCGCCCCAGGACCTTGGCGTGACCCACGGCCGTGAGGCCC
  CCAATAACGCTTTTGGTGTTCTCCTAACCCCCAGTCTGCCCGCCGCGCGAAAAAATTTTT
  CCCCTTTGAATAAAAAACCGGTCCCTTGGGCCTCTCTAAGTCGCAAACCCGCGGAAGCCT
  ATTTCTAGCAAAGCCTTTCTCCATCCCCCCCCAAATATTATCCCTCCAAGCTTTGGTAAA
  CAGTCTTTATATTCGTTCACCCCTCACGCCAACTCCGCCAAAGCCCCTTCAACAAACATC
  TTATTTAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004393
• Insert Size: 4230 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004393.1, NP_004384.1
• RefSeq Size: 5493 bp
• RefSeq ORF: 2688 bp
• Locus ID: 1605
• Cytogenetics: 3p21.31
• Domains: CADG
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Hypertrophic cardiomyopathy (HCM), Viral myocarditis
• Gene Summary: 'This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. [provided by RefSeq, Nov 2015]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.