Alpha Dystroglycan (DAG1) (NM_004393) Human Untagged Clone
CAT#: SC117393
DAG1 (untagged)-Human dystroglycan 1 (dystrophin-associated glycoprotein 1) (DAG1), transcript variant 2
"NM_004393" in other vectors (6)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | DAG1 |
Synonyms | 156DAG; A3a; AGRNR; DAG; LGMDR16; MDDGA9; MDDGC7; MDDGC9 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_004393, the custom clone sequence may differ by one or more nucleotides
ATGAGGATGTCTGTGGGCCTCTCGCTGCTGCTGCCCCTCTGGGGGAGGACCTTTCTCCTCCTGCTCTCTG TGGTTATGGCTCAGTCCCACTGGCCCAGTGAACCCTCAGAGGCTGTCAGGGACTGGGAAAACCAGCTTGA GGCATCCATGCACTCAGTGCTCTCAGACCTCCACGAGGCTGTTCCCACAGTGGTTGGCATTCCTGATGGC ACGGCTGTCGTCGGGCGCTCATTTCGAGTGACCATTCCAACAGATTTGATTGCCTCCAGTGGAGATATCA TCAAGGTATCAGCGGCAGGGAAGGAGGCTTTGCCATCTTGGCTGCACTGGGACTCACAGAGCCACACCCT GGAGGGCCTCCCCCTTGACACTGATAAGGGTGTGCATTACATTTCAGTGAGCGCTACACGGCTGGGGGCC AACGGGAGCCACATCCCCCAGACCTCCAGTGTGTTCTCCATCGAGGTCTACCCTGAAGACCACAGTGAGC TGCAGTCGGTGAGGACAGCCTCCCCAGACCCTGGTGAGGTGGTATCATCTGCCTGTGCTGCGGATGAACC TGTGACTGTTTTGACGGTGATTTTGGATGCCGACCTCACCAAGATGACCCCAAAGCAAAGGATTGACCTC CTGCACAGGATGCGGAGCTTCTCAGAAGTAGAGCTTCACAACATGAAATTAGTGCCGGTGGTGAATAACA GACTATTTGACATGTCGGCCTTCATGGCTGGCCCGGGAAATGCAAAAAAGGTGGTGGAGAATGGGGCCCT TCTCTCCTGGAAGCTGGGCTGCTCCCTGAACCAGAACAGTGTGCCTGACATTCATGGTGTAGAGGCCCCT GCCAGGGAGGGCGCAATGTCTGCTCAGCTTGGCTACCCTGTGGTGGGTTGGCACATCGCCAATAAGAAGC CCCCTCTTCCCAAACGCGTCCGGAGGCAGATCCATGCTACACCCACACCTGTCACTGCCATTGGGCCCCC AACCACGGCTATCCAGGAGCCCCCATCCAGGATCGTGCCAACCCCCACATCTCCAGCCATTGCTCCTCCA ACAGAGACCATGGCTCCTCCAGTCAGGGATCCTGTTCCTGGGAAACCCACGGTCACCATCCGGACTCGAG GCGCCATTATTCAAACCCCAACCCTAGGCCCCATCCAGCCTACTCGGGTGTCAGAAGCTGGCACCACAGT TCCTGGCCAGATTCGCCCAACGATGACCATTCCTGGCTATGTGGAGCCTACTGCAGTTGCTACCCCTCCC ACAACCACCACCAAGAAGCCACGAGTATCCACACCAAAACCAGCAACGCCTTCAACTGACTCCACCACCA CCACGACTCGCAGGCCAACCAAGAAACCACGGACACCCCGGCCAGTGCCCCGGGTCACCACCAAAGTTTC CATCACCAGATTGGAAACTGCCTCACCGCCTACTCGTATTCGCACCACCACCAGTGGAGTGCCCCGTGGC GGAGAACCCAACCAGCGCCCAGAGCTCAAGAACCATATTGACAGGGTAGATGCCTGGGTTGGCACCTACT TTGAGGTGAAGATCCCGTCAGACACTTTCTATGACCATGAGGACACCACCACTGACAAGCTGAAGCTGAC CCTGAAACTGCGGGAGCAGCAGCTGGTGGGCGAGAAGTCCTGGGTACAGTTCAACAGCAACAGCCAGCTC ATGTATGGCCTTCCCGACAGCAGCCACGTGGGCAAACACGAGTATTTCATGCATGCCACAGACAAGGGGG GCCTGTCGGCTGTGGATGCCTTCGAGATCCACGTCCACAGGCGCCCCCAAGGGGATAGGGCTCCTGCAAG GTTCAAGGCCAAGTTTGTGGGTGACCCGGCACTGGTGTTGAATGACATCCACAAGAAGATTGCCTTGGTA AAGAAACTGGCCTTCGCCTTTGGAGACCGAAACTGTAGCACCATCACCCTGCAGAATATCACCCGGGGCT CCATCGTGGTGGAATGGACCAACAACACACTGCCCTTGGAGCCCTGCCCCAAGGAGCAGATCGCTGGGCT GAGCCGCCGGATCGCTGAGGATGATGGAAAACCTCGGCCTGCCTTCTCCAACGCCCTAGAGCCTGACTTT AAGGCCACAAGCATCACTGTGACGGGCTCTGGCAGTTGTCGGCACCTACAGTTTATCCCTGTGGTACCAC CCAGGAGAGTGCCCTCAGAGGCGCCGCCCACAGAAGTGCCTGACAGGGACCCTGAGAAGAGCAGTGAGGA TGATGTCTACCTGCACACAGTCATTCCGGCCGTGGTGGTCGCAGCCATCCTGCTCATTGCTGGCATCATT GCCATGATCTGCTACCGCAAGAAGCGGAAGGGCAAGCTTACCCTTGAGGACCAGGCCACCTTCATCAAGA AGGGGGTGCCTATCATCTTTGCAGACGAACTGGACGACTCCAAGCCCCCACCCTCCTCCAGCATGCCACT CATTCTGCAGGAGGAGAAGGCTCCCCTACCCCCTCCTGAGTACCCCAACCAGAGTGTGCCCGAGACCACT CCTCTGAACCAGGACACCATGGGAGAGTACACGCCCCTGCGGGATGAGGATCCCAATGCGCCTCCCTACC AGCCCCCACCGCCCTTCACAGCACCCATGGAGGGCAAGGGCTCCCGTCCCAAGAACATGACCCCATACCG GTCACCTCCTCCCTATGTCCCACCTTAA >OriGene 5' read for NM_004393 unedited
CTTTGGATTTTGTAACACGACTCACTATGGGCGGCACGCGATTCGGCACGAGGGCGGCGC TCGCGCCTCTTATGCTTGGCGGTGGCGGCGGCGGCAGCTTCGCGCCGAATCCCCGGGGAG CGGCGGTGGCGGCGTCCTGGGGCCAGGAGGAGCGAACACCTGCCGCATTCCTCCCGCCGG CGCTGGGCTCTGTGTGCTCCGGGATGGAGCAGGTGTGCAGAGGGTGAGAACCCAGCTCTG GGACCAAGTCACTTGCTTCCTTACTTAGCAAGACTATCGACTTGAGCAAACTTGGACCTG GGATGAGGATGTCTGTGGGCCTCTCGCTGCTGCTGCCCCTCTGGGGGAGGACCTTTCTCC TCCTGCTCTCTGTGGTTATGGCTCAGTCCCACTGGCCCAGTGAACCCTCAGAGGCTGTCA GGGACTGGGAAAACCAGCTTGAGGCATCCATGCACTCAGTGCTCTCAGACCTCCACGAGG CTGTTCCCACAGTGGTTGGCATTCCTGATGGCACGGCTGTCGTCGGGCGCTCATTTCGAG TGACCATTCCAACAGATTTGATTGCCTCCAGTGGAGATATCATCAAGGTATCAGCGGCAG GGAAGGAGGCTTTGCCATCTTGGCTGCACTGGGACTCACAGAGCCACACCCTGGAGGGCC TCCCCCTTGACACTGTAAGGGTGTGCATTACATTTCAGTGAGCGCTACACGGCTGGNGGC CAACGGGAGCCACATCCNCCAGACCTCCAGTGTGTTCTCAATCGAGGTCTACCCTGGAGA CACAGTGAGCTGCAGTCGGTGGAGGACAGCCTCCCAGACCTGGGGGAGGTGTATCATCTG CCTGTGCTGGGGATGACCTGTGACTGTTTTGACGGTGGATTTGGAATGCCGCCCTTACAA GATGACCCCAAAACAAAGAATGACCTCCTGCACAGGATGCGGACCT >OriGene 3' read for NM_004393 unedited
TTTAGCTATGGCACGCGGCACGCAATCTAGTATCGAGTTCTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTCCCAATTAAAATCTTTTTTTTAATAAAACAATTGGAGGCTGTATAAA AACTTTAGAAAAAAATTACCTTTGAAAGCTAACAAATTTTTATTATGAACTATGGCTGGC ACTCCTCCCCAGCCGCCAAACCTTTTCCAGTATTTCAGGCCCAAGGCCTGGGACTGGAGG GCCAAAAAAAAGGGATGGGGGGGGTCGGGGCAGGACTGGGGACTGTTAGGACAAAGCGGC CAAGGTCAAGGGAGGAAAGAGCATCCCATGACATAAAAATATTGCTTCTTCACCAAAATG TCCCCCACTGAGGGTTTCAAAAAACCGGGATATTCCTTTTAGGAGGAAAAAAAAAACCAA GGCCCAAAAAAGGGGTAAAACAAAATAGGTTGATGGGGGGGAAACCTTTGACCTCCCAGG GGCCCCCACAAGCAGTTGGCCCACCCTGGAGCACCAAAGTTGGGGGCCACAGCCACCATT GCCTTTTGGAGTCCCTTTTTCATGGAAATGAAAACCCCTTGGCCGGGGCCCACTAGTGTA CATGGGCTGGAAGGGGGGATGCCCACGGCCCAAGAAACCCCCCCAGCTTCCACCCCCTGG TATTAGTGGGTGATCAAACCCCGCCCCAGGACCTTGGCGTGACCCACGGCCGTGAGGCCC CCAATAACGCTTTTGGTGTTCTCCTAACCCCCAGTCTGCCCGCCGCGCGAAAAAATTTTT CCCCTTTGAATAAAAAACCGGTCCCTTGGGCCTCTCTAAGTCGCAAACCCGCGGAAGCCT ATTTCTAGCAAAGCCTTTCTCCATCCCCCCCCAAATATTATCCCTCCAAGCTTTGGTAAA CAGTCTTTATATTCGTTCACCCCTCACGCCAACTCCGCCAAAGCCCCTTCAACAAACATC TTATTTAC |
Restriction Sites | NotI-NotI |
ACCN | NM_004393 |
Insert Size | 4230 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004393.1, NP_004384.1 |
RefSeq Size | 5493 bp |
RefSeq ORF | 2688 bp |
Locus ID | 1605 |
Cytogenetics | 3p21.31 |
Domains | CADG |
Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Hypertrophic cardiomyopathy (HCM), Viral myocarditis |
Gene Summary | 'This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. [provided by RefSeq, Nov 2015]' Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201121 | DAG1 (Myc-DDK-tagged)-Human dystroglycan 1 (dystrophin-associated glycoprotein 1) (DAG1), transcript variant 2 |
USD 420.00 |
|
RG201121 | DAG1 (GFP-tagged) - Human dystroglycan 1 (dystrophin-associated glycoprotein 1) (DAG1), transcript variant 2 |
USD 460.00 |
|
RC201121L1 | Lenti-ORF clone of DAG1 (Myc-DDK-tagged)-Human dystroglycan 1 (dystrophin-associated glycoprotein 1) (DAG1), transcript variant 2 |
USD 620.00 |
|
RC201121L2 | Lenti-ORF clone of DAG1 (mGFP-tagged)-Human dystroglycan 1 (dystrophin-associated glycoprotein 1) (DAG1), transcript variant 2 |
USD 620.00 |
|
RC201121L3 | Lenti-ORF clone of DAG1 (Myc-DDK-tagged)-Human dystroglycan 1 (dystrophin-associated glycoprotein 1) (DAG1), transcript variant 2 |
USD 620.00 |
|
RC201121L4 | Lenti-ORF clone of DAG1 (mGFP-tagged)-Human dystroglycan 1 (dystrophin-associated glycoprotein 1) (DAG1), transcript variant 2 |
USD 620.00 |
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