Dystrophia myotonica protein kinase (DMPK) (NM_004409) Human Untagged Clone

CAT#: SC117404

DMPK (untagged)-Human dystrophia myotonica-protein kinase (DMPK), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DMPK
• Synonyms: DM; DM1; DM1PK; DMK; MDPK; MT-PK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004409 edited
  ATGTCAGCCGAGGTGCGGCTGAGGCGGCTCCAGCAGCTGGTGTTGGACCCGGGCTTCCTG
  GGGCTGGAGCCCCTGCTCGACCTTCTCCTGGGCGTCCACCAGGAGCTGGGCGCCTCCGAA
  CTGGCCCAGGACAAGTACGTGGCCGACTTCTTGCAGTGGGCGGAGCCCATCGTGGTGAGG
  CTTAAGGAGGTCCGACTGCAGAGGGACGACTTCGAGATTCTGAAGGTGATCGGACGCGGG
  GCGTTCAGCGAGGTAGCGGTAGTGAAGATGAAGCAGACGGGCCAGGTGTATGCCATGAAG
  ATCATGAACAAGTGGGACATGCTGAAGAGGGGCGAGGTGTCGTGCTTCCGTGAGGAGAGG
  GACGTGTTGGTGAATGGGGACCGGCGGTGGATCACGCAGCTGCACTTCGCCTTCCAGGAT
  GAGAACTACCTGTACCTGGTCATGGAGTATTACGTGGGCGGGGACCTGCTGACACTGCTG
  AGCAAGTTTGGGGAGCGGATTCCGGCCGAGATGGCGCGCTTCTACCTGGCGGAGATTGTC
  ATGGCCATAGACTCGGTGCACCGGCTTGGCTACGTGCACAGGGACATCAAACCCGACAAC
  ATCCTGCTGGACCGCTGTGGCCACATCCGCCTGGCCGACTTCGGCTCTTGCCTCAAGCTG
  CGGGCAGATGGAACGGTGCGGTCGCTGGTGGCTGTGGGCACCCCAGACTACCTGTCCCCC
  GAGATCCTGCAGGCTGTGGGCGGTGGGCCTGGGACAGGCAGCTACGGGCCCGAGTGTGAC
  TGGTGGGCGCTGGGTGTATTCGCCTATGAAATGTTCTATGGGCAGACGCCCTTCTACGCG
  GATTCCACGGCGGAGACCTATGGCAAGATCGTCCACTACAAGGAGCACCTCTCTCTGCCG
  CTGGTGGACGAAGGGGTCCCTGAGGAGGCTCGAGACTTCATTCAGCGGTTGCTGTGTCCC
  CCGGAGACACGGCTGGGCCGGGGTGGAGCAGGCGACTTCCGGACACATCCCTTCTTCTTT
  GGCCTCGACTGGGATGGTCTCCGGGACAGCGTGCCCCCCTTTACACCGGATTTCGAAGGT
  GCCACCGACACATGCAACTTCGACTTGGTGGAGGACGGGCTCACTGCCATGGTGAGCGGG
  GGCGGGGAGACACTGTCGGACATTCGGGAAGGTGCGCCGCTAGGGGTCCACCTGCCTTTT
  GTGGGCTACTCCTACTCCTGCATGGCCCTCAGGGACAGTGAGGTCCCAGGCCCCACACCC
  ATGGAACTGGAGGCCGAGCAGCTGCTTGAGCCACACGTGCAAGCGCCCAGCCTGGAGCCC
  TCGGTGTCCCCACAGGATGAAACAGCTGAAGTGGCAGTTCCAGCGGCTGTCCCTGCGGCA
  GAGGCTGAGGCCGAGGTGACGCTGCGGGAGCTCCAGGAAGCCCTGGAGGAGGAGGTGCTC
  ACCCGGCAGAGCCTGAGCCGGGAGATGGAGGCCATCCGCACGGACAACCAGAACTTCGCC
  AGTCAACTACGCGAGGCAGAGGCTCGGAACCGGGACCTAGAGGCACACGTCCGGCAGTTG
  CAGGAGCGGATGGAGTTGCTGCAGGCAGAGGGAGCCACAGCTGTCACGGGGGTCCCCAGT
  CCCCGGGCCACGGATCCACCTTCCCATATGGCCCCCCGGCCGTGGCTGTGGGCCAGTGCC
  CGCTGGTGGGGCCAGGCCCCATGCACCGCCGCCACCTGCTGCTCCCTGCCAGGGTCCCTA
  GGCCTGGCCTATCGGAGGCGCTTTCCCTGCTCCTGTTCGCCGTTGTTCTGTCTCGTGCCG
  CCGCCCTGGGCTGCATTGGGTTGGTGGCCCACGCCGGCCAACTCACCGCAGTCTGGCGCC
  GCCCAGGAGCCGCCCGCGCTCCCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004409 unedited
  CAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGACAGCCA
  GGGACAGGCAGACATGCAGCCAGGGCTCCAGGGCCTGGACAGGGGCTGCCAGGCCCTGTG
  ACAGGAGGACCCCGAGCCCCCGGCCCGGGGAGGGGCCATGGTGCTGCCTGTCCAACATGT
  CAGCCGAGGTGCGGCTGAGGCGGCTCCAGCAGCTGGTGTTGGACCCGGGCTTCCTGGGGC
  TGGAGCCCCTGCTCGACCTTCTCCTGGGCGTCCACCAGGAGCTGGGCGCCTCCGAACTGG
  CCCAGGACAAGTACGTGGCCGACTTCTTGCAGTGGGCGGAGCCCATCGTGGTGAGGCTTA
  AGGAGGTCCGACTGCAGAGGGACGACTTCGAGATTCTGAAGGTGATCGGACGCGGGGCGT
  TCAGCGAGGTAGCGGTAGTGAAGATGAAGCAGACGGGCCAGGTGTATGCCATGAAGATCA
  TGAACAAGTGGGACATGCTGAAGAGGGGCGAGGTGTCGTGCTTCCGTGAGGAGAGGGACG
  TGTTGGTGAATGGGGACCGGCGGTGGATCACGCAGCTGCACTTCGCCTTCCAGGATGAGA
  ACTACCTGTACCTGGTCATGGAGTATTACGTGGGCGGNGACCTGCTGACACTGCTGAGCA
  AGTTTGGGGAGCGGATTCCGGCCGAGATGGCGCGCTTCTACCTGGCGGAGATTGTCATGG
  CCATAGACTCGGTGCACCGGCTTGGCTACGTGCACAGGGACATCAAACCCGACACATNCT
  GCTGGACCGCTGTGGCCACATNCGNCTGGCCGACTTCNGCTCTTGCCTCAAGCTGCNGGC
  AGATGGAACCNGTGCNGTCGCTGGNTGGCTGTGGGCACCCCANACTACCCTGTCCCCGAG
  ATCCTGCAGCTGTGGNNCNGTGGGCCCTGGACANNGCACTACGGNCCCNNATGTGACTGG
  TGGCGCCTGGGNTGTATCGCCTATGAATGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004409 unedited
  TATCTATGNNACCGCGGCCGCAATTCTANGATCGATTTTTTTTTTTTTTTTTTTTTTTTG
  GGCAGAAGGAGGGCCTTTTATTCGCGAGGGTCGGGGGTGGGGGTCCTAGGTGGGGACAGA
  CAATAAATACCGAGGAATGTCGGGGTCTCAGTGCATCCAAAACGTGGATTGGGGTTGTTG
  GGGGTCCTGTAGCCTGTCAGCGAGTCGGAGGACGAGGTCAATAAATATCCAAACCGCCGA
  AGCGGGCGGAGCCGGCTGGGGCTCCGAGAGCAGCATAAGTGAGGAGGGGGGCGCGGGATC
  CCCGAAAAAGCGGGTTTGGCAAAAGCAAATTTCCCGAGTAAGCAGGCAGAGATCGCGCCA
  GACGCTCCCCAGAGCAGGGCGTCATGCACAAGAAAGCTTTGCACTTTGCGAACCAACCAT
  AGGTGGGGGTGCGTGGAGGATGGAACACGGACGGCCCGGCTTGCTGCCTTCCCAGGCCTG
  CAGTTTGCCCATCCACGTCAGGGCCTCAGCCTGGCCGAAAGAAAGAAATGGTCTGTGATC
  CCCCCAGCAGCAGCAGCAGCATTCCCGGCTACAAGGACCCTTCGAGCCCCGTTCGCCGGC
  CGCGGACCCGGCCCCTCCCTCCCCGGCCGCTAGGGGGCGGGCCCGGATCACAGGACTGGA
  GCTGGGCGGAGACCCACGCTCGGAGCGGTTGTGAACTGGCAGGCGGTGGGCGCGGCTTCT
  GTGCCGTGCCCCGGGCACTCAGTCTCCAACGGGGCCCCCGAGTCGAAGAAGTTCTAGGGT
  TCAAGGAACGCGGGCGGCTCCTGGGCGGCGCCAGACTGCGGTGAGTTTCCCCGCGTGGGC
  CACACACCAATGGAGCCCAAGCGGCGGGCCGAGAAAAACAACGGGGAACAGGAGCAAGGA
  AAGCCCCTCGATAGCCAGGCTTAGGACCCTGCAGGGAGCAACAGGTGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004409
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004409.2, NP_004400.4
• RefSeq Size: 2768 bp
• RefSeq ORF: 1890 bp
• Locus ID: 1760
• Cytogenetics: 19q13.32
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'The protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-38 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2016]'
  Transcript Variant: This variant (2) has multiple differences in the presence and absence of exons at its 5' end, compared to variant 1. These differences produce a distinct 5' UTR and cause translation initiation at an alternative start codon, compared to variant 1. The encoded protein (isoform 2, also known as isoform 9) has a distinct N-terminus and is shorter than isoform 1.