DUSP1 (NM_004417) Human Untagged Clone

CAT#: SC117410

DUSP1 (untagged)-Human dual specificity phosphatase 1 (DUSP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DUSP1
• Synonyms: CL100; HVH1; MKP-1; MKP1; PTPN10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004417 edited
  GAATTCGGCACGGGCGCAGAGAGACCCGGGGGTCTAGCTTTTCCTCGAAAAGCGCCGCCC
  TGCCCTTGGCCCCGAGAACAGACAAAGAGCACCGCAGGGCCGATCACGCTGGGGGCGCTG
  AGGCCGGCCATGGTCATGGAAGTGGGCACCCTGGACGCTGGAGGCCTGCGGGCGCTGCTG
  GGGGAGCGAGCGGCGCAATGCCTGCTGCTGGACTGCCGCTCCTTCTTCGCTTTCAACGCC
  GGCCACATCGCCGGCTCTGTCAACGTGCGCTTCAGCACCATCGTGTGGCGCCGGGCCAAG
  GGCGCCATGGGCCTGGAGCACATCGTGCCCAACGCCGAGCTCCGCGGCCGCCTGCTGGCC
  GGCGCCTACCACGCCGTGGTGTTGCTGGACGAGCGCAGCGCCGCCCTGGACGGCGCCAAG
  CGCGACGGCACCCTGGCCCTGGCGGCCGGCGCGCTCTGCCGCGAGGCGCGCGCCGCGCAA
  GTCTTCTTCCTCAAAGGAGGATACGAAGCGTTTTCGGCTTCCTGCCCGGAGCTGTGCAGC
  AAACAGTCGACCCCCATGGGGCTCAGCCTTCCCCTGAGTACTAGCGTCCCTGACAGCGCG
  GAATCTGGGTGCAGTTCCTGCAGTACCCCACTCTACGATCAGGGTGGCCCGGTGGAAATC
  CTGCCCTTTCTGTACCTGGGCAGTGCGTATCACGCTTCCCGCAAGGACATGCTGGATGCC
  TTGGGCATAACTGCCTTGATCAACGTCTCAGCCAATTGTCCCAACCATTTTGAGGGTCAC
  TACCAGTACAAGAGCATCCCTGTGGAGGACAACCACAAGGCAGACATCAGCTCCTGGTTC
  AACGAGGCCATTGACTTCATAGACTCCATCAAGAATGCTGGAGGAAGGGTGTTTGTCCAC
  TGCCAGGCAGGCATTTCCCGGTCAGCCACCATCTGCCTTGCTTACCTTATGAGGACTAAT
  CGAGTCAAGCTGGACGAGGCCTTTGAGTTTGTGAAGCAGAGGCGAAGCATCATCTCTCCC
  AACTTCAGCTTCATGGGCCAGCTGCTGCAGTTTGAGTCCCAGGTGCTGGCTCCGCACTGT
  TCGGCAGAGGCTGGGAGCCCCGCCATGGCTGTGCTCGACCGAGGCACCTCCACCACCACC
  GTGTTCAACTTCCCCGTCTCCATCCCTGTCCACTCCACGAACAGTGCGCTGAGCTACCTT
  CAGAGCCCCATTACGACCTCTCCCAGCTGCTGAAAGGCCACGGGAGGTGAGGCTCTTCAC
  ATCCCATTGGGACTCCATGCTCCTTGAGAGGAGAAATGCAATAACTCTGGGAGGGGCTCG
  AGAGGGCTGGTCCTTATTTATTTAACTTCACCCGAGTTCCTCTGGGTTTCTAAGCAGTTA
  TGGTGATGACTTAGCGTCAAGACATTTGCTGAACTCAGCACATTCGGGACCAATATATAG
  TGGGTACATCAAGTCCATCTGACAAAATGGGGCAGAAGAGAAAGGACTCAGTGTGTGATC
  CGGTTTCTTTTTGCTCGCCCCTGTTTTTTGTAGAATCTCTTCATGCTTGACATACCTACC
  AGTATTATTCCCGACGACACATATACATATGAGAATATACCTTATTTATTTTTGTGTAGG
  TGTCTGCCTTCACAAATGTCATTGTCTACTCCTAGAAGAACCAAATACCTCAATTTTTGT
  TTTTGAGTACTGTACTATCCTGTAAATATATCTTAAGCAGGTTTGTTTTCAGCACTGATG
  GAAAATACCAGTGTTGGGTTTTTTTTTAGTTGCCAACAGTTGTATGTTTGCTGATTATTT
  ATGACCTGAAATAATATATTTCTTCTTCTAAGAAGACATTTTGTTACATAAGGATGACTT
  TTTTATACAATGGAATAAATTATGGCATTTCTATTGAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004417 unedited
  CAAAATTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGGGCGCAGAGAGACCC
  GGGGGTCTAGCTTTTCCTCGAAAGCGCCGCCCTGCCCTTGGCCCCGAGAACAGACAAAGA
  GCACCGCAGGGCCGATCACGCTGGGGGCGCTGAGGCCGGCCATGGTCATGGAAGTGGGCA
  CCCTGGACGCTGGAGGCCTGCGGGCGCTGCTGGGGGAGCGAGCGGCGCAATGCCTGCTGC
  TGGACTGCCGCTCCTTCTTCGCTTTCAACGCCGGCCACATCGCCGGCTCTGTCAACGTGC
  GCTTCAGCACCATCGTGTGGCGCCGGGCCAAGGGCGCCATGGGCCTGGAGCACATCGTGC
  CCAACGCCGAGCTCCGCGGCCGCCTGCTGGCCGGCGCCTACCACGCCGTGGTGTTGCTGG
  ACGAGCGCAGCGCCGCCCTGGACGGCGCCAAGCGCGACGGCACCCTGGCCCTGGCGGCCG
  GCGCGCTCTGCCGCGAGGCGCGCGCCGCGCAAGTCTTCTTCCTCAAAGGAGGATACGAAG
  CGTTTTCGGCTTCCTGCCCGGAGCTGTGCAGCAAACAGTCGACCCCCATGGGGCTCAGCC
  TTCCCCTGAGTACTAGCGTCCCTGACAGCGCGGAATCTGGGTGCAGTTCCTGCAGTACCC
  CACTCTACGATCAGGGTGGCCCGGTGGAAATCCTGCCCTTTCTGTACCTGGGCAGTGCGT
  ATCACGCTTCCCGCAGGACATGCTGGATGCCTTGGCATAACTGCCTTGATCAACGTCTCA
  GCCAATTGTCCCACCATTTTTGGGGTCACTACCAGTACAGAGCATCCCGGTGGGAGACAC
  CCCCAAGCAGACTCAGCTCCTGGTTAACGAGCCATTGACTCATAACTCATCAGAGCTGAG
  GAAAGGGGGGTGGTCCACTGCAGCAGCATTCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004417 unedited
  AATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAATAAAAATGCCA
  TAATTTATTCCATTGTATAAAAAAGTCATCCTTATGTAACAAAATGTCTTCTTAAAAAAA
  AAAATATATTATTTCAGGTCATAAATAATCAGCAAACATACAACTGTTGGCAACTAAAAA
  AAAACCCAACACTGGTATTTTCCATCAGGGCTGAAAACAAACCTGCTTAAAATATATTTA
  CAGGATAGTACAGTCCTCAAAAACAAAAATTGAGGTATTTGGTTCTTCTAGGAGTAAACA
  ATGACATTTGTGAAGGCAAACCCCTACACAAAAATAAATAAGGTATATTCTCATATGTAT
  ATGGGCCGCCGGGAATAATACTGGTAGGTATGTCAAGCATGAAAAGATTCTACAAAAAAC
  AGGGGCGAGCAAAAAGAAACCGGATCACACACTGAGTCCTTTCTCTTCTGCCCCATTTTG
  TCAAATGGACTTGATGTACCCACTATATATTGGTCCCGAATGTGCTGAGTTCAGCAAATG
  TCTTGACGCTAAGTCATCACCATAACTGCTTAGAAACCCAGAGGAACTCGGGTGAAGTTA
  AATAAATAAGGACCAGCCCTCTAGAGCCCTTCCCAGATTATTGCATTTCTCCTCTCAAGA
  GCATGGAGTCCCAATGGGATGTGAAGAGCCTCACCTCCCGTGGCCTTTCANCAGCTGGGA
  AAAGGTCCTAATGGGGCTCTGAAGGTANCTCACCCCCCTTGTTCTGGAGTGAACAGGGAT
  GGAACCGGGAANTTAACANCGGGGTGGTGGAGGTGCTTCGTCCAACCAGCCTGCGGGGCT
  CCCACCTTTGCCAAAGTGCGAACCACACCTGGACTTAATGCNACACTGCCCATGAATTAA
  TTGGGAAATTATCTCCTCTTGTTAAAAATAAAGGCTTGCCACTTGCTCCATATCCCTAAG
  GAACAAGCAAATGGGCTACCGAAATCCTCTGAATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004417
• Insert Size: 1880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004417.2, NP_004408.1
• RefSeq Size: 2015 bp
• RefSeq ORF: 1104 bp
• Locus ID: 1843
• Cytogenetics: 5q35.1
• Domains: DSPc, RHOD, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'The protein encoded by this gene is a phosphatase with dual specificity for tyrosine and threonine. The encoded protein can dephosphorylate MAP kinase MAPK1/ERK2, which results in its involvement in several cellular processes. This protein appears to play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. Finally, the encoded protein can make some solid tumors resistant to both chemotherapy and radiotherapy, making it a target for cancer therapy. [provided by RefSeq, Aug 2017]'