CDC25B (NM_004358) Human Untagged Clone

CAT#: SC117412

CDC25B (untagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC25B
• Synonyms: cell division cycle 25 homolog B (S. pombe); cell division cycle 25B; OTTHUMP00000030137; OTTHUMP00000030138; OTTHUMP00000030139
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004358, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGTGCCCCAGCCGGAGCCCGCGCCAGGCTCGGCTCTCAGTCCAGCAGGCGTGTGCGGTGGCGCCC
  AGCGTCCGGGCCACCTCCCGGGCCTCCTGCTGGGATCTCATGGCCTCCTGGGGTCCCCGGTGCGGGCGGC
  CGCTTCCTCGCCGGTCACCACCCTCACCCAGACCATGCACGACCTCGCCGGGCTCGGCAGCCGCAGCCGC
  CTGACGCACCTATCCCTGTCTCGACGGGCATCCGAATCCTCCCTGTCGTCTGAATCCTCCGAATCTTCTG
  ATGCAGGTCTCTGCATGGATTCCCCCAGCCCTATGGACCCCCACATGGCGGAGCAGACGTTTGAACAGGC
  CATCCAGGCAGCCAGCCGGATCATTCGAAACGAGCAGTTTGCCATCAGACGCTTCCAGTCTATGCCGGTG
  AGGCTGCTGGGCCACAGCCCCGTGCTTCGGAACATCACCAACTCCCAGGCGCCCGACGGCCGGAGGAAGA
  GCGAGGCGGGCAGTGGAGCTGCCAGCAGCTCTGGGGAAGACAAGGAGAATGATGGATTTGTCTTCAAGAT
  GCCATGGAAGCCCACACATCCCAGCTCCACCCATGCTCTGGCAGAGTGGGCCAGCCGCAGGGAAGCCTTT
  GCCCAGAGACCCAGCTCGGCCCCCGACCTGATGTGTCTCAGTCCTGACCGGAAGATGGAAGTGGAGGAGC
  TCAGCCCCCTGGCCCTAGGTCGCTTCTCTCTGACCCCTGCAGAGGGGGATACTGAGGAAGATGATGGATT
  TGTGGACATCCTAGAGAGTGACTTAAAGGATGATGATGCAGTTCCCCCAGGCATGGAGAGTCTCATTAGT
  GCCCCACTGGTCAAGACCTTGGAAAAGGAAGAGGAAAAGGACCTCGTCATGTACAGCAAGTGCCAGCGGC
  TCTTCCGCTCTCCGTCCATGCCCTGCAGCGTGATCCGGCCCATCCTCAAGAGGCTGGAGCGGCCCCAGGA
  CAGGGACACGCCCGTGCAGAATAAGCGGAGGCGGAGCGTGACCCCTCCTGAGGAGCAGCAGGAGGCTGAG
  GAACCTAAAGCCCGCGTCCTCCGCTCAAAATCACTGTGTCACGATGAGATCGAGAACCTCCTGGACAGTG
  ACCACCGAGAGCTGATTGGAGATTACTCTAAGGCCTTCCTCCTACAGACAGTAGACGGAAAGCACCAAGA
  CCTCAAGTACATCTCACCAGAAACGATGGTGGCCCTATTGACGGGCAAGTTCAGCAACATCGTGGATAAG
  TTTGTGATTGTAGACTGCAGATACCCCTATGAATATGAAGGCGGGCACATCAAGACTGCGGTGAACTTGC
  CCCTGGAACGCGACGCCGAGAGCTTCCTACTGAAGAGCCCCATCGCGCCCTGTAGCCTGGACAAGAGAGT
  CATCCTCATTTTCCACTGTGAATTCTCATCTGAGCGTGGGCCCCGCATGTGCCGTTTCATCAGGGAACGA
  GACCGTGCTGTCAACGACTACCCCAGCCTCTACTACCCTGAGATGTATATCCTGAAAGGCGGCTACAAGG
  AGTTCTTCCCTCAGCACCCGAACTTCTGTGAACCCCAGGACTACCGGCCCATGAACCACGAGGCCTTCAA
  GGATGAGCTAAAGACCTTCCGCCTCAAGACTCGCAGCTGGGCTGGGGAGCGGAGCCGGCGGGAGCTCTGT
  AGCCGGCTGCAGGACCAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004358 unedited
  TGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGAGGGTGCCCCAGCCGGAGC
  CCGCGCCAGGCTCGGCTCTCAGTCCAGCAGGCGTGTGCGGTGGCGCCCAGCGTCCGGGCC
  ACCTCCCGGGCCTCCTGCTGGGATCTCATGGCCTCCTGGGGTCCCCGGTGCGGGCGGCCG
  CTTCCTCGCCGGTCACCACCCTCACCCAGACCATGCACGACCTCGCCGGGCTCGGCAGCG
  AAACCCCAAAGAGTCAGGTAGGGACCCTGCTCTTCCGCAGCCGCAGCCGCCTGACGCACC
  TATCCCTGTCTCGACGGGCATCCGAATCCTCCCTGTCGTCTGAATCCTCCGAATCTTCTG
  ATGCAGGTCTCTGCATGGATTCCCCCAGCCCTATGGACCCCCACATGGCGGAGCACACGT
  TTGAACAGGCCATCCAGGCAGCCAGCCGGATCATTCGAAACGAGCAGTTTGCCATCAGAC
  GCTTCCAGTCTATGCCGGTGAGGCTGCTGGGCCACAGCCCCGTGCTTCGGAACATCACCA
  ACTCCCAGGCGCCCGACGGCCGGAGGAAGAGCGAGGCGGGCAGTGGAGCTGCCAGCAGCT
  CTGGGGAAGACAAGGAGAATGATGGATTTGTCTTCAAGATGCCCTGGAAAGCCCACACAT
  CCCAGCTCCCACCCATGCTCTGGCAGAGTGGGCCAGCCGCAGGGAAGCCTTTGCCCAGAG
  ACCCAGCTCGGCCCCCGACCTGATGTGTCTCAGTCCTGACCGGAAGATGGAAATGGAGGA
  GCTCACCCCCCTGGCCCCTAGTCGCTTCTCTCTGACCCTGCCGNNAGGGATACCTGAGAA
  AGAGATGGCATTTGTGACCTCCCACAGAGGGACTTAAAGGATGATGATGCCACTTCCCCC
  AGCCATGGAAAGTCTTATTAGTGCCCCCACTGGCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004358 unedited
  TGTACCGCGGCCCGCTTTTCTATATCGAGTTTTTTTTTTTTTTTTTTCTCAACTTGCTCA
  TTGCTTTATTGAACCAAGGCCCTAACAGATGACTCAGCAGGGCCTTCAAGCACAGCCCTG
  CCCCCGTCTTGAGATTCAGAATCCAGAGGGTGCTCAGTCCTTGGTTTATCTGCTTCTGTG
  ACATTTCCTCTTGAATAGCTACAAACCCTAAGTGTAAATATTTTTGTCCACACAAATAGG
  CACACATATATTCCTGCTGCAGGCTGTCATGCTCAACAAAACGCTCCCACCTGGTTTGGG
  TATGCAAGGCACTGCGCATCCACGGCCATCCACGGCCATCCACCCATCCATCCAACCTTC
  CATCCATCCGCAACAAGACAGCAGCAAGTTCTGAGCACTCAGGCCCCAGGGTTCACAGCA
  GTAAGGGCTGGGCTTCCATCAGGCTCATGATTCAGGCCTTAACCCTTGCCCTGGGAGCCC
  CCAATGATAATTAACATCCCTTTCTGGGGCAACAAAACTGGGACCGATTGGGTAACCTGT
  ATTTGACAGGGGAACCTAAACAGTCTACGGAAGTTGCCTGAGATGGCCGTAAAGCCAAAC
  GGACCATAGCCACAGGTCACACTGAAATAGGAAAGTAGTAAAGCCTCTGTTCAAGCTGAA
  GGTGCCTCGTATGGTGGCACCCGACAAGGGGAAAACCAAAGGGGGCCCTCTGGCAATCTT
  CCCTCGTTTCTCACATAAATAGGTGCCCCCCTGGCCCGAGTTTTCCACAAGCATTAAATC
  TGCTCTTCAGGCCACGAAGCATCTACCCTCGACCATTCCTTAGTAACACGGTTCTCCCCC
  ACTCTCCTCGTCCCCCACCTTGGATCCCCTCCGACCCCGCTAAAGAAGGTTCTCCTTACA
  GATACAAAAACCCTCGGGATCAATATTGTCTTTTCTGTGTTACCACTAATCACTTACATA
  CGGTCTTCAGGGGGGGGCTCCATATCCCACTCCTATTAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004358
• Insert Size: 2930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004358.3, NP_004349.1
• RefSeq Size: 3659 bp
• RefSeq ORF: 1701 bp
• Locus ID: 994
• Cytogenetics: 20p13
• Domains: RHOD
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Cell cycle, MAPK signaling pathway, Progesterone-mediated oocyte maturation
• Gene Summary: 'CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2), also known as CDC25B1, uses an alternate splice site in the 5' coding region, compared to variant 1, resulting in a shorter protein (isoform 2) compared to isoform 1.