E Cadherin (CDH1) (NM_004360) Human Untagged Clone

CAT#: SC117413

CDH1 (untagged)-Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDH1
• Synonyms: Arc-1; BCDS1; CD324; CDHE; ECAD; LCAM; UVO
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004360, the custom clone sequence may differ by one or more nucleotides
  
  
  ACCAGGCTTGCGGAAGTCAGTTCAGACTCCAGCCCGCTCCAGCCCGGCCCGACCCGACCGCACCCGGCGC
  CTGCCCTCGCTCGGCGTCCCCGGCCAGCCATGGGCCCTTGGAGCCGCAGCCTCTCGGCGCTGCTGCTGCT
  GCTGCAGGTCTCCTCTTGGCTCTGCCAGGAGCCGGAGCCCTGCCACCCTGGCTTTGACGCCGAGAGCTAC
  ACGTTCACGGTGCCCCGGCGCCACCTGGAGAGAGGCCGCGTCCTGGGCAGAGTGAATTTTGAAGATTGCA
  CCGGTCGACAAAGGACAGCCTATTTTTCCCTCGACACCCGATTCAAAGTGGGCACAGATGGTGTGATTAC
  AGTCAAAAGGCCTCTACGGTTTCATAACCCACAGATCCATTTCTTGGTCTACGCCTGGGACTCCACCTAC
  AGAAAGTTTTCCACCAAAGTCACGCTGAATACAGTGGGGCACCACCACCGCCCCCCGCCCCATCAGGCCT
  CCGTTTCTGGAATCCAAGCAGAATTGCTCACATTTCCCAACTCCTCTCCTGGCCTCAGAAGACAGAAGAG
  AGACTGGGTTATTCCTCCCATCAGCTGCCCAGAAAATGAAAAAGGCCCATTTCCTAAAAACCTGGTTCAG
  ATCAAATCCAACAAAGACAAAGAAGGCAAGGTTTTCTACAGCATCACTGGCCAAGGAGCTGACACACCCC
  CTGTTGGTGTCTTTATTATTGAAAGAGAAACAGGATGGCTGAAGGTGACAGAGCCTCTGGATAGAGAACG
  CATTGCCACATACACTCTCTTCTCTCACGCTGTGTCATCCAACGGGAATGCAGTTGAGGATCCAATGGAG
  ATTTTGATCACGGTAACCGATCAGAATGACAACAAGCCCGAATTCACCCAGGAGGTCTTTAAGGGGTCTG
  TCATGGAAGGTGCTCTTCCAGGAACCTCTGTGATGGAGGTCACAGCCACAGACGCGGACGATGATGTGAA
  CACCTACAATGCCGCCATCGCTTACACCATCCTCAGCCAAGATCCTGAGCTCCCTGACAAAAATATGTTC
  ACCATTAACAGGAACACAGGAGTCATCAGTGTGGTCACCACTGGGCTGGACCGAGAGAGTTTCCCTACGT
  ATACCCTGGTGGTTCAAGCTGCTGACCTTCAAGGTGAGGGGTTAAGCACAACAGCAACAGCTGTGATCAC
  AGTCACTGACACCAACGATAATCCTCCGATCTTCAATCCCACCACGTACAAGGGTCAGGTGCCTGAGAAC
  GAGGCTAACGTCGTAATCACCACACTGAAAGTGACTGATGCTGATGCCCCCAATACCCCAGCGTGGGAGG
  CTGTATACACCATATTGAATGATGATGGTGGACAATTTGTCGTCACCACAAATCCAGTGAACAACGATGG
  CATTTTGAAAACAGCAAAGGGCTTGGATTTTGAGGCCAAGCAGCAGTACATTCTACACGTAGCAGTGACG
  AATGTGGTACCTTTTGAGGTCTCTCTCACCACCTCCACAGCCACCGTCACCGTGGATGTGCTGGATGTGA
  ATGAAGCCCCCATCTTTGTGCCTCCTGAAAAGAGAGTGGAAGTGTCCGAGGACTTTGGCGTGGGCCAGGA
  AATCACATCCTACACTGCCCAGGAGCCAGACACATTTATGGAACAGAAAATAACATATCGGATTTGGAGA
  GACACTGCCAACTGGCTGGAGATTAATCCGGACACTGGTGCCATTTCCACTCGGGCTGAGCTGGACAGGG
  AGGATTTTGAGCACGTGAAGAACAGCACGTACACAGCCCTAATCATAGCTACAGACAATGGTTCTCCAGT
  TGCTACTGGAACAGGGACACTTCTGCTGATCCTGTCTGATGTGAATGACAACGCCCCCATACCAGAACCT
  CGAACTATATTCTTCTGTGAGAGGAATCCAAAGCCTCAGGTCATAAACATCATTGATGCAGACCTTCCTC
  CCAATACATCTCCCTTCACAGCAGAACTAACACACGGGGCGAGTGCCAACTGGACCATTCAGTACAACGA
  CCCAACCCAAGAATCTATCATTTTGAAGCCAAAGATGGCCTTAGAGGTGGGTGACTACAAAATCAATCTC
  AAGCTCATGGATAACCAGAATAAAGACCAAGTGACCACCTTAGAGGTCAGCGTGTGTGACTGTGAAGGGG
  CCGCCGGCGTCTGTAGGAAGGCACAGCCTGTCGAAGCAGGATTGCAAATTCCTGCCATTCTGGGGATTCT
  TGGAGGAATTCTTGCTTTGCTAATTCTGATTCTGCTGCTCTTGCTGTTTCTTCGGAGGAGAGCGGTGGTC
  AAAGAGCCCTTACTGCCCCCAGAGGATGACACCCGGGACAATGTTTATTACTATGATGAAGAAGGAGGCG
  GAGAAGAGGACCAGGACTTTGACTTGAGCCAGCTGCACAGGGGCCTGGACGCTCGGCCTGAAGTGACTCG
  TAACGACGTTGCACCAACCCTCATGAGTGTCCCCCGGTATCTTCCCCGCCCTGCCAATCCCGATGAAATT
  GGAAATTTTATTGATGAAAATCTGAAAGCGGCTGATACTGACCCCACAGCCCCGCCTTATGATTCTCTGC
  TCGTGTTTGACTATGAAGGAAGCGGTTCCGAAGCTGCTAGTCTGAGCTCCCTGAACTCCTCAGAGTCAGA
  CAAAGACCAGGACTATGACTACTTGAACGAATGGGGCAATCGCTTCAAGAAGCTGGCTGACATGTACGGA
  GGCGGCGAGGACGACTAGGGGACTCGAGAGAGGCGGGCCCCAGACCCATGTGCTGGGAAATGCAGAAATC
  ATGTTGCTGGTGGTTTTTCAGCTCCCTTCCCTTGAGATGAGTTTCTGG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004360 unedited
  GGGTTGCAGNATTTTGTAATACGATTCACTATAGGCGGCCCGCGAATCGGCACCAGGCTT
  GCGGTAGTCAGTTCAGACTCCAGCCCGCTCCAGCCCGGCCCGACCCGACCGCACCCGGCG
  CCTGCCCTCGCTCGGCGTCCCCGGCCAGCCATGGGCCCTTGGAGCCGCAGCCTCTCGGCG
  CTGCTGCTGCTGCTGCAGGTCTCCTCTTGGCTCTGCCAGGAGCCGGAGCCCTGCCACCCT
  GGCTTTGACGCCGAGAGCTACACGTTCACGGTGCCCCGGCGCCACCTGGAGAGAGGCCGC
  GTCCTGGGCAGAGTGAATTTTGAAGATTGCACCGGTCGACAAAGGACAGCCTATTTTTCC
  CTCGACACCCGATTCAAAGTGGGCACAGATGGTGTGATTACAGTCAAAAGGCCTCTACGG
  TTTCATAACCCACAGATCCATTTCTTGGTCTACGCCTGGGACTCCACCTACAGAAAGTTT
  TCCACCAAAGTCACGCTGAATACAGTGGGGCACCACCACCGCCCCCCGCCCCATCAGGCC
  TCCGTTTCTGGAATCCAAGCAGAATTGCTCACATTTCCCAACTCCTCTCCTGGCCTCAGA
  AGACAGAAGAGAGACTGGGTTATTCCTCCCATCAGCTGCCCAGAAAATGAAAAAGGCCCA
  TTTCCTAAAAACCTGGTTCAGATCAAATCCAACAAAGACAAAGAAGGCAAGGTTTTCTAC
  AGCATCACTGGCCAAGGAGCTGACACACCCCCTGTTGGTGTCTTTATTATTTGAAAGAGA
  ACAGTATGGCTGAAGGTGACAGAGCCTCTGCATAGAGAACGCACTGCCCCATACACTCTC
  TTTTCTCACGCTGTGTCATCCAACGGAAATGCCGTTGAGGATCCATGGAGAATTTGATCA
  CGGTACCGATCAAATGACAACAGCCCGAATCACCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004360 unedited
  TATGGACCGCGGCCGCATCTAAATCGAGTTTTTTTTTTTTTTTTTTAATGGTTTAACAAA
  ATTGTTTAATAAAATTTATAAAAATGCATCTTTGAGAATACTTTTCTCAGCTTGAATTGT
  TTTCCTTTTCCACCCCCAAAGAAAATACACAATTATCAGCACCCACACATGTATACACTC
  AAAACTACAGTGACATTCTCTACACAGAACTATATTCGATATAGCTTGAACTGCCGAAAA
  ATCAAGACAATTCCAAAAAGTGATTGCAGGGTTGATTTTTTTCTCCAAAACACTCGAGAA
  ACAGTAAAGCTATTTGAACAAAAGACTTTTGTTTGATTGCCAAAAGTCGAAATCCACATT
  TAAATGTACCGAGATGCAACGTCCGATGCCCGCTACCCTTTTTCCCCGCCCCACCCCCGT
  TTTTGGACCCACCCTCTTCACCGTTTTCCCCCCCTTTGTCCTTTTTTTCTTTTGCCTCCC
  CCCCCCCCCCCCTTTTTCCCCCGCTCCAGCCTAGTTCGCCCGCATATCCCCACCCCGCCC
  CGCCCATTGTCTCCCGCCTTGTGTGCCCAGCTGCCACCGCCCTACCCCCACTCCCCCGCG
  TATCTGCCAGATGTTGTTCGCGCGGCGGGGCGTCGCGGGGAGGTGCGCCCCCCGCCCCTC
  CATTCTGTCGCCGGTTTGCAGTCCCTCTTGTGTGCTTTTTTAGCGCGGGTGGCCTCCGTG
  GGGCAACCCGCCCACCCGTCCGCCCCTCTTTGTCTCCTCCAGTCTTGTACATAATCTCCA
  TAGTATGCCCTGAAGTCGTCGCTCCCACCCCCCCGCACCCATTGCGCCCCCTCGCGGTTC
  TCCCGCCTCATCTTGCCCCACTCCGTCGTCCCGTCGCGTTTCTCAACCAGTGGCGTTCTC
  ACCCCGCATCATTTCACCGCCCCCTCTTGTCCCAGCCCCCTTTGCTCCACCGCGGTTACG
  TCAGACCCTCACGTATTCCGCCGTCCGGGCCCTCCCGCTAGCCCACTTGCCGCCCTACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004360
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004360.2, NP_004351.1
• RefSeq Size: 4815 bp
• RefSeq ORF: 4815 bp
• Locus ID: 999
• Cytogenetics: 16q22.1
• Domains: Cadherin_C_term, CA
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Cell adhesion molecules (CAMs), Endometrial cancer, Melanoma, Pathogenic Escherichia coli infection, Pathways in cancer, Thyroid cancer
• Gene Summary: 'This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).