RhoGDI (ARHGDIA) (NM_004309) Human Untagged Clone

CAT#: SC117424

ARHGDIA (untagged)-Human Rho GDP dissociation inhibitor (GDI) alpha (ARHGDIA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARHGDIA
• Synonyms: GDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004309, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGAGCAGGAGCCCACAGCCGAGCAGCTGGCCCAGATTGCAGCGGAGAACGAGGAGGATGAGCACT
  CGGTCAACTACAAGCCCCCGGCCCAGAAGAGCATCCAGGAGATCCAGGAGCTGGACAAGGACGACGAGAG
  CCTGCGAAAGTACAAGGAGGCCCTGCTGGGCCGCGTGGCCGTTTCCGCAGACCCCAACGTCCCCAACGTC
  GTGGTGACTGGCCTGACCCTGGTGTGCAGCTCGGCCCCGGGCCCCCTGGAGCTGGACCTGACGGGCGACC
  TGGAGAGCTTCAAGAAGCAGTCGTTTGTGCTGAAGGAGGGTGTGGAGTACCGGATAAAAATCTCTTTCCG
  GGTTAACCGAGAGATAGTGTCCGGCATGAAGTACATCCAGCATACGTACAGGAAAGGCGTCAAGATTGAC
  AAGACTGACTACATGGTAGGCAGCTATGGGCCCCGGGCCGAGGAGTACGAGTTCCTGACCCCCGTGGAGG
  AGGCACCCAAGGGTATGCTGGCCCGGGGCAGCTACAGCATCAAGTCCCGCTTCACAGACGACGACAAGAC
  CGACCACCTGTCCTGGGAGTGGAATCTCACCATCAAGAAGGACTGGAAGGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004309 unedited
  GGTCGAAATTTGTAAACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTGAACCGC
  GCGGCCGAACCCTCCGGTGTCCCGACCCAGGCTAAGCTTGAGCATGGCTGAGCAGGAGCC
  CACAGCCGAGCAGCTGGCCCAGATTGCAGCGGAGAACGAGGAGGATGAGCACTCGGTCAA
  CTACAAGCCCCCGGCCCAGAAGAGCATCCAGGAGATCCAGGAGCTGGACAAGGACGACGA
  GAGCCTGCGAAAGTACAAGGAGGCCCTGCTGGGCCGCGTGGCCGTTTCCGCAGACCCCAA
  CGTCCCCAACGTCGTGGTGACTGGCCTGACCCTGGTGTGCAGCTCGGCCCCGGGCCCCCT
  GGAGCTGGACCTGACGGGCGACCTGGAGAGCTTCAAGAAGCAGTCGTTTGTGCTGAAGGA
  GGGTGTGGAGTACCGGATAAAAATCTCTTTCCGGGTTAACCGAGAGATAGTGTCCGGCAT
  GAAGTACATCCAGCATACGTACAGGANAGGCGTCAAGATTGACAAGACTGACTACATGGT
  AGGCAGCTATGGGCCCCGGGCCGAGGAGTACGAGTTCCTGACCCCCGTGGAGGANGCACC
  CAAGGGTATGCTGGCCCGGNGCAGCTACAGCATCAAGTCCCGCTTCACAGACGACGACNA
  GACCGACCACCTGTCCTGGGAGTGGAATCTCACCATCAAGAAAGACTGGNAGGACTGAGC
  CCAGCCCAGAGCGGNCAGGNCAGACTGACNGACGGACGACGGACAGGCNGATGTGTTCCC
  CCCAGCCCCTCCCTCCCCATACAAAGTGCTGACAGCCCCTNCGTGCCCCTCCCACCCTGG
  TCCGCCTCCCTGGCCTGGCTCAACCGAGTGCCTCCGACCCCCCTCCTTAGCCCTCCCCCA
  CCCACAGGCCCAGCTCCTCGGNCTCTGGCTCGTTGCTGCTCTGCCTGGGCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004309 unedited
  CCGCGGCCGCATTCTAAGTCGAGNNNTTNTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTT
  AGGGGCATTTATTTCCCGGTCAGAAAAGAGCAGGGACAGGCGCCTCTGCCTGAGCCTGGC
  AAACACAACACGAAAACCGGGGATGGGGCGGGGGAGGCACAGGAAACGGCTCTCAGCAAT
  GTGTGCACTTGGTCCCTTGTTTGTTCCTGGCTGGGTCAGGGAAGGCCTGCCGGGGGTGGT
  GGCACTGAAAGCCTGGGGGAACAGGCGCCAGGCCAGTGGCCCAGGGACGGCACGGACGGA
  GGCAATAAATACTGATGGCCAGGCGGGGCTCCTGGCTGGGCCCAGGTGGGGTGGGTGAGG
  CTGTCCATCGAGGGCTCTTGGGGGGGTGTGGGCTCTGGGCACTGCCCGCTGAGACAGAAA
  AGGCAGAGGCAGGACAATACCCAGCCTCCTGGATGGTACTGAGGTGACTTGAGTTTTGGC
  AATTTGGCTTTCCCCAAGCCGCCGGAGCCATCTCCACAGCCCTGTCCAGGGAAGGGGGCA
  GGCTGGCCAGGCACTGGTGGGCTGGGGAGCAGCAGCAGCACACCCCACGTGTCCCTGGGT
  CACTGGGTTCGCCACCGGGGAAGGGACGGGGAGGCCACATGCTCATGCAGACACAGGGAG
  TTAGAGGGCTAGTGAGGCCCCACGGTACACTCCACATGTAANGGCATCATGTNTAGACGG
  NACCGACAGCGACAAGGGGGCTGGCCAGGGACCANCGGGGCTGGAGGACGGGCCGGCCCC
  CCAGAGCCGTGCATCCCACACCCCATTTCACCCCGGAGCAGCAGACGCACACGTCCAGGG
  GCACCACCGGGCCTGAAAATGCTGCTCCGCTCCTCCTGAACCAGCCTCGGNTGTGACGGN
  GAGAAAACCTGGGGGCACANAAAGGGCANNAAGNCTGNNTGNGCCTTTCTCCCACGCACA
  GCNAANCCCACNAACAGGAACGAGAGCTGGCCCTGGGTGGGNAAGCTAGAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004309
• Insert Size: 1920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004309.3, NP_004300.1
• RefSeq Size: 1912 bp
• RefSeq ORF: 615 bp
• Locus ID: 396
• Cytogenetics: 17q25.3
• Domains: Rho_GDI
• Protein Families: Druggable Genome
• Protein Pathways: Neurotrophin signaling pathway
• Gene Summary: 'This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]'
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform (a).