Bone marrow stromal cell antigen 1 (BST1) (NM_004334) Human Untagged Clone

CAT#: SC117438

BST1 (untagged)-Human bone marrow stromal cell antigen 1 (BST1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BST1
• Synonyms: CD157
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117438 sequence for NM_004334 edited (data generated by NextGen Sequencing)
  ATGGCGGCCCAGGGGTGCGCGGCATCGCGGCTGCTCCAGCTGCTGCTGCAGCTTCTGCTT
  CTACTGTTGCTGCTGGCGGCGGGCGGGGCGCGCGCGCGGTGGCGCGGGGAGGGCACCAGC
  GCACACTTGCGGGACATCTTCCTGGGCCGCTGCGCCGAGTACCGCGCACTGCTGAGTCCC
  GAGCAGCGGAACAAGAACTGCACAGCCATCTGGGAAGCCTTTAAAGTGGCGCTGGACAAG
  GATCCCTGCTCCGTGCTGCCCTCAGACTATGACCTTTTTATTAACTTGTCCAGGCACTCT
  ATTCCCAGAGATAAGTCCCTGTTCTGGGAAAATAGCCACCTCCTTGTTAACAGCTTTGCA
  GACAACACCCGTCGTTTTATGCCCCTGAGCGATGTTCTGTATGGCAGGGTTGCAGATTTC
  TTGAGCTGGTGTCAACAGAAAAATGACTCTGGACTCGATTACCAATCCTGCCCTACATCA
  GAAGACTGTGAAAATAATCCTGTGGATTCCTTTTGGAAAAGGGCATCCATCCAGTATTCC
  AAGGATAGTTCTGGGGTGATCCACGTCATGCTGAATGGTTCAGAGCCAACAGGAGCCTAT
  CCCATCAAAGGTTTTTTTGCAGATTATGAAATTCCAAACCTCCAGAAGGAAAAAATTACA
  CGAATCGAGATCTGGGTTATGCATGAAATTGGGGGACCCAATGTGGAATCCTGCGGGGAA
  GGCAGCATGAAAGTCCTGGAAAAGAGGCTGAAGGACATGGGGTTCCAGTACAGCTGTATT
  AATGATTACCGACCAGTGAAGCTCTTACAGTGCGTGGACCACAGCACCCATCCTGACTGT
  GCCTTAAAGTCGGCAGCAGCCGCTACTCAAAGAAAAGCCCCAAGTCTTTATACAGAACAA
  AGGGCGGGTCTTATCATTCCCCTCTTTCTGGTGCTGGCTTCCCGGACTCAACTGTAA
  
  Clone variation with respect to NM_004334.2
  434 g=>a;943 a=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004334 unedited
  NGGTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGNAGCACGGG
  ACTGGAGGGACCAAAGTTCCCCGATGGCGGCCCAGGGGTGCGCGGCATCGCGGCTGCTCC
  AGCTGCTGCTGCAGCTTCTGCTTCTACTGTTGCTGCTGGCGGCGGGCGGGGCGCGCGCGC
  GGTGGCGCGGGGAGGGCACCAGCGCACACTTGCGGGACATCTTCCTGGGCCGCTGCGCCG
  AGTACCGCGCACTGCTGAGTCCCGAGCAGCGGAACAAGAACTGCACAGCCATCTGGGAAG
  CCTTTAAAGTGGCGCTGGACAAGGATCCCTGCTCCGTGCTGCCCTCAGACTATGACCTTT
  TTATTAACTTGTCCAGGCACTCTATTCCCAGAGATAAGTCCCTGTTCTGGGAAAATAGCC
  ACCTCCTTGTTAACAGCTTTGCAGACAACACCCGTCGTTTTATGCCCCTGAGCGATGTTC
  TGTATGGCAGGGTTGCAGATTTCTTGAGCTGGTGTCAACAGAAAAATGACTCTGGACTCG
  ATTACCAATCCTGCCCTACATCAGAAGACTGTGAAAATAATCCTGTGGATTCCTTTTGGA
  AAAGGGCATCCATCCAGTATTCCAAGGATAGTTCTGGNGTGATCCACGTCATGCTGAATG
  GTTCAGAGCCAACAGGAGCCTATCCCATCAAAGGTTTTTTTGCAGATTATGAAATTCCNA
  ACCTNCAGAAGGAAAAAATTACACGAAATCGAGATCTGGGTTATGCATGAAATTGGGGGA
  CCCCATGTGGAATCCTGCGGGGAAAGCAGCATGAAAATCCTGGAAAAGAAGCTGAAGGAC
  ATGGGGTTCCAGTACAGCTGTTTTATTGATACCGACCAGTGAAACTCTTAAGGTGCCTGG
  AACACAGCACCCATTCTGACTGTGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004334 unedited
  NCGCCTATTATGNACGCGGCCGCTTTCTANGATCGGTTTTTTTTTTTTTTTTTTTGAGTA
  AAAACAGCTTTATTAGAATTCTCTTAAAATGTTTGATAATATACAAGCAGTCTGACTCCC
  AATGCTTTGTGTCATTATACAAATAAGAAAATAACTTGCTTTAATTCTAATGCAGCATTT
  TTTAACCTGCTAAATAGAACTAACTTCTTGTTTCTGAAATCCTGAACATATGCCTCATTG
  AAATACCATTTTCAGGACATCGTTTTCCCAGCATAAAAGCTTCTTTAGATAACAGAATCA
  TTTGGTATACACAGAACACGAATGATGACTGCAAGGGGAGGCTGCAGGGCTGGAGGAGGG
  TTAGAGCAACACAGTTTCCAGTTACAGTTGAGTCCGGGAAGCCAGCACCAGAAAGAGGGG
  AATGATAAGACCCGCCCTTTGTTCTGTATAAAGACTTGGGGCTTTTCTTTGAGTAGCGGC
  TGCTGCCGACTTTAAGGCACAGTCAGGATGGGTGCTGTGGTCCACGCACTGTAAGAGCTT
  CACTGGTCGGTAATCATTAATACAGCTGTACTGGAACCCCATGTCCTTCAGCCTCTTTTC
  CAGGACTTTCATGCTGCCTTCCCCGCANGATTCCACATTGGGTCCCCCAATTTCATGCAT
  AACCCAGATCTCGATTCGTGTATTTTTTCCTTCTGGAAGTTTGGAATTTCATAATCTGCA
  AAAAAACTTTGATGGGATAGGCTCCTGTTGGCTCTGAACCATTCAGCATGACGTGGATCA
  CCCCAAACTATCCTTGGGATACTGGATGGATGCCCCTTTTCCAAAAGGATCCACAGGATA
  TTTTCACAGTCTCCTGATGTANGGCCAGATTGGTAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004334
• Insert Size: 1250 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004334.1, NP_004325.1
• RefSeq Size: 1411 bp
• RefSeq ORF: 957 bp
• Locus ID: 683
• Cytogenetics: 4p15.32
• Domains: Rib_hydrolayse
• Protein Families: Transmembrane
• Protein Pathways: Calcium signaling pathway, Metabolic pathways, Nicotinate and nicotinamide metabolism
• Gene Summary: 'Bone marrow stromal cell antigen-1 is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that facilitates pre-B-cell growth. The deduced amino acid sequence exhibits 33% similarity with CD38. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B-cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population. [provided by RefSeq, Jul 2008]'