Caspase 3 (CASP3) (NM_004346) Human Untagged Clone

CAT#: SC117447

CASP3 (untagged)-Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CASP3
• Synonyms: CPP32; CPP32B; SCA-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117447 sequence for NM_004346 edited (data generated by NextGen Sequencing)
  ATGGAGAACACTGAAAACTCAGTGGATTCAAAATCCATTAAAAATTTGGAACCAAAGATC
  ATACATGGAAGCGAATCAATGGACTCTGGAATATCCCTGGACAACAGTTATAAAATGGAT
  TATCCTGAGATGGGTTTATGTATAATAATTAATAATAAGAATTTTCATAAAAGCACTGGA
  ATGACATCTCGGTCTGGTACAGATGTCGATGCAGCAAACCTCAGGGAAACATTCAGAAAC
  TTGAAATATGAAGTCAGGAATAAAAATGATCTTACACGTGAAGAAATTGTGGAATTGATG
  CGTGATGTTTCTAAAGAAGATCACAGCAAAAGGAGCAGTTTTGTTTGTGTGCTTCTGAGC
  CATGGTGAAGAAGGAATAATTTTTGGAACAAATGGACCTGTTGACCTGAAAAAAATAACA
  AACTTTTTCAGAGGGGATCGTTGTAGAAGTCTAACTGGAAAACCCAAACTTTTCATTATT
  CAGGCCTGCCGTGGTACAGAACTGGACTGTGGCATTGAGACAGACAGTGGTGTTGATGAT
  GACATGGCGTGTCATAAAATACCAGTGGAGGCCGACTTCTTGTATGCATACTCCACAGCA
  CCTGGTTATTATTCTTGGCGAAATTCAAAGGATGGCTCCTGGTTCATCCAGTCGCTTTGT
  GCCATGCTGAAACAGTATGCCGACAAGCTTGAATTTATGCACATTCTTACCCGGGTTAAC
  CGAAAGGTGGCAACAGAATTTGAGTCCTTTTCCTTTGACGCTACTTTTCATGCAAAGAAA
  CAGATTCCATGTATTGTTTCCATGCTCACAAAAGAACTCTATTTTTATCACTAA
  
  Clone variation with respect to NM_004346.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004346 unedited
  ATATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCGCGGCTCCT
  AGCGGATGGGTGCTATTGTGAGGCGGTTGTGGAAGTTAATAAAGGTATCCATGNGAGAAC
  ACTNGAAAACTCAGTGGATTCAAAATCCATTAAAAATTTGGAACCAAAGATCATACATGG
  AAGCGAATCAATGGACTCTGGAATATCCCTGGACAACAGTTATAAAATGGATTATCCTGA
  GATGGGTTTATGTATAATAATTAATAATAAGAATTTTCATAAAAGCACTGGAATGACATC
  TCGGTCTGGTACAGATGTCGATGCAGCAAACCTCAGGGAAACATTCAGAAACTTGAAATA
  TGAAGTCAGGAATAAAAATGATCTTACACGTGAAGAAATTGTGGAATTGATGCGTGATGT
  TTCTAAAGAAGATCACAGCAAAAGGAGCAGTTTTGTTTGTGTGCTTCTGAGCCATGGTGA
  AGAAGGAATAATTTTTGGAACAAATGGACCTGTTGACCTGAAAAAAATAACAAACTTTTT
  CAGAGGGGATCGTTGTAGAAGTCTAACTGGAAAACCCAAACTTTTCATTATTCAGGCCTG
  CCGTGGTACAGAACTGGACTGTGGCATTGAGACAGACAGTGGTGTTGATGATGACATGGC
  GTGTCATAAAATACCAGTGGAGGCCGACTTCTTGTATGCATACTCCACAGCACCTGGTTA
  TTATTCTTGGCGAAATCANAGGATGGCTCCTGGTTCATCCAGTCGCTTTGTGCCATGCTG
  AAACAGTATGCCGGACAGCTTGAAATTATGCACATTCTTACCCGGGTAACCGCAAGGTGG
  GCACAGAATTTGAGTCCCTTTCCTTTGACGCTACTTTTCATGCAAAGAAACAGATTCCAT
  GGATTGGTTCCATGCTCACCAAAGAACTCTATTTNTATCACTAAAGAAAAGGGTGGNTTG
  GTGGGGTTTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004346 unedited
  ACTATGAACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTAATTTTAAGTTTGAATGTATATTTTGGAAATAAAAATGGAAAA
  TAACAAATTTGTACAAAATTGTCACATAAAAACACACTTTAAAATACCATTACATGCTGG
  GGGTATTTACAAAAGTTACAGGTCTAGTAAAAAAAAAAAAACTGTCATTGACAAATGCGA
  GCTCATTTTTTGAGCAACAAAAAATCTCCCGTGAAATGTCATACTGACAGCCAGTGAAAC
  TTGGTGCAGTGACGGCTCCCCACCTGCTGAGGCCTGGAGCCTGCCTCCCGGGCTGAGTGC
  TCAACTTTGGCCTCCGGCTGGATGCCGTCTAAAGTCCTATGGGCTCATAGGTCTTTTTTT
  TTCAGTAAACAGTATAAAACATTACTATTTTCCTTTGATTGGAAACTTCAACAGAAAATA
  ACATACTTAAAAATTTTGCTACAAAAAGTACTATGATTTTTATTGCCTCACCACCTTTAA
  AACATTTCCACTTCACAAGTTTCTGATTTGCTTCACTTTCTTACTTGGCGATGGCGCCCT
  GGCAGCATCATCCACACATACCAGTGCGTATGGAAAAATGGGCTGTAGGCCCTTCCTGCG
  TGGTCCATGGCTCTGCCTTCATGGAACAGGCTGAGTCATGTCTCTGCTCAGGCTCAAACC
  ATCTACTCTCAAGTTACAGGCAGCTTAGCCCATTCATTTATTGCTTTCCTTATATTCCAC
  TGTTTATTATTATAAAGTTGGATTTCCATATGTTCCCTATAGAAATTATTCCACATTACC
  CTGCCTAATAATCCAACAAGTTGGGGGGTTTTTATATAATAGCTCTCAACATTATTGCTG
  GTTTTTGAAGCCTCACTGAATGCCTAGGGACAATCAAAGTATTTTTGCTTGGAGATCAGG
  GCATTGTACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004346
• Insert Size: 2740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004346.3, NP_004337.2
• RefSeq Size: 2689 bp
• RefSeq ORF: 834 bp
• Locus ID: 836
• Cytogenetics: 4q35.1
• Domains: CASc, ICE_p10, ICE_p20
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, Huntington's disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Viral myocarditis
• Gene Summary: 'The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]'
  Transcript Variant: This variant (1, also known as alpha) encodes the longest isoform (a). Variants 1, 2, and 3 all encode the same isoform (a).