RUNX1T1 (NM_004349) Human Untagged Clone

CAT#: SC117449

RUNX1T1 (untagged)-Human runt-related transcription factor 1, translocated to, 1 (cyclin D-related) (RUNX1T1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RUNX1T1
• Synonyms: AML1-MTG8; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004349, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTGATCGTACTGAGAAGCACTCCACAATGCCAGACTCACCTGTGGATGTGAAGACGCAATCTAGGC
  TGACTCCTCCAACAATGCCACCTCCCCCAACTACTCAAGGAGCTCCAAGAACCAGTTCATTTACACCGAC
  AACGTTAACTAATGGCACGAGCCATTCTCCTACAGCCTTGAATGGCGCCCCCTCACCACCCAATGGCTTC
  AGCAATGGGCCTTCCTCTTCTTCCTCCTCCTCTCTGGCTAATCAACAGCTGCCCCCAGCCTGTGGTGCCA
  GGCAACTCAGCAAGCTGAAAAGGTTCCTTACTACCCTGCAGCAGTTTGGCAATGACATTTCACCCGAGAT
  AGGAGAAAGAGTTCGCACCCTCGTTCTGGGACTAGTGAACTCCACTTTGACAATTGAAGAATTTCATTCC
  AAACTGCAAGAAGCTACTAACTTCCCACTGAGACCTTTTGTCATCCCATTTTTGAAGGCCAACTTGCCCC
  TGCTGCAGCGTGAGCTCCTCCACTGCGCAAGACTGGCCAAACAGAACCCTGCCCAGTACCTCGCCCAGCA
  TGAACAGCTGCTTCTGGATGCCAGCACCACCTCACCTGTTGACTCCTCAGAGCTGCTTCTCGATGTGAAC
  GAAAACGGGAAGAGGCGAACTCCAGACAGAACCAAAGAAAATGGCTTTGACAGAGAGCCTTTGCACTCAG
  AACATCCAAGCAAGCGACCATGCACTATTAGCCCAGGCCAGCGGTACAGTCCAAATAACGGCTTATCCTA
  CCAGCCCAATGGCCTGCCTCACCCTACCCCACCTCCACCTCAGCATTACCGTTTGGATGATATGGCCATT
  GCCCACCACTACAGGGACTCCTATCGACACCCCAGCCACAGGGACCTCAGGGACAGAAACAGACCTATGG
  GGTTGCATGGCACACGTCAAGAAGAAATGATTGATCACAGACTAACAGACAGAGAATGGGCAGAAGAGTG
  GAAACATCTTGACCATCTGTTAAACTGCATAATGGACATGGTAGAAAAAACAAGGCGATCTCTCACCGTA
  CTAAGGCGGTGTCAAGAAGCAGACCGGGAAGAATTGAATTACTGGATCCGGCGGTACAGTGACGCCGAGG
  ACTTAAAAAAAGGTGGCGGCAGTAGCAGCAGCCACTCTAGGCAGCAGAGTCCCGTCAACCCAGACCCAGT
  TGCACTAGACGCGCATCGGGAATTCCTTCACAGGCCTGCGTCTGGATACGTGCCAGAGGAGATCTGGAAG
  AAAGCTGAGGAGGCCGTCAATGAGGTGAAGCGCCAGGCGATGACGGAGCTGCAGAAGGCCGTGTCTGAGG
  CGGAGCGGAAAGCCCACGACATGATCACAACAGAGAGGGCCAAGATGGAGCGCACGGTCGCCGAGGCCAA
  ACGGCAGGCGGCGGAGGACGCACTGGCAGTTATCAATCAGCAGGAGGATTCAAGCGAGAGTTGCTGGAAT
  TGTGGCCGTAAAGCGAGTGAAACCTGCAGTGGCTGTAACACAGCCCGATACTGTGGCTCATTTTGCCAGC
  ACAAAGACTGGGAGAAGCACCATCACATCTGTGGACAGACCCTGCAGGCCCAGCAGCAGGGAGACACACC
  TGCAGTCAGCTCCTCTGTCACGCCCAACAGCGGGGCTGGGAGCCCGATGGACACACCACCAGCAGCCACT
  CCGAGGTCAACCACCCCGGGAACCCCTTCCACCATAGAGACAACCCCTCGCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004349 unedited
  CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGACACAATG
  TGCCATCCTGACAAGGCTTTTACTTCTGATAAGCTCCAATGTGTGTTTAATGAATACAAA
  GCCGCGGTCTGGGTGCCGCCTCGGCCGCGGCCGCTCTCCCGCGCTCCTTTGCCAGAAGCT
  GGCGGAGAGAATTTACATTTAAAGATTAGCAGAGTGAGAAAGAGAAATCTGCCTTTTGTT
  GTGTGGGGTGAGGAGGAGGCATCTACCCCTGGCCTTGACGCTATCTCCCATCACCTCTGC
  TATCCAGACAGGACTCACCGAGAAAAGATTGATCTCTGGGCTGGTGAACATAATCTCTGT
  CCCAGTCAGAAAAGGAGAGAGGAAATTAGCAGAGCGATTGGTGGAGAATGATATCTGTCA
  AAAGAAACACTTGGAGAGCACTGAGTTTAGTAATAGGTGACTGCCGGAAAAAAGGGAACT
  TTGAATATTGTCAAGGCGCACGCGCCCAGCACCCTGCCTCCGGCGATGCCGCCCCAGCCC
  CTCTGATGGCCCTCCTCTCTGCTGCCACTCATTCCAGAACAGGAGGCATGAGCCCGGAAC
  GCGCTTGCTTTTAGGAGACAGCCACTTTCTGTGTGGTACGCTGGATTCAAGGATGCCTGA
  TCGTACTGAGAAGCACTCCACAATGCCAGACTCACCTGTGGATGTGAAGACGCAATCTAG
  GCTGACTCCTCCAACAATGCCACCTCCCCCAACTACTCAAGGAGCTCCAAGAACCAGTTC
  ATTTACACCCGACACGTTAACTAATGGCACGAGCCATTCTCCTACAGCCTTGAATGGCGC
  CCCCTCACCACCCATGGCTTCAGCAATGGGCCCTTNCTCTTCTTCTCCTNCTCTCTGGCT
  AATCACAGCTGCCCCCAGCCTGTGGTGCCCAGCACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004349 unedited
  NNNNNNNNNNNNNTTTTTGACTTTGGACCGCGGNCCGCATNCTAGGATCGAGTTTTTTTT
  TTTTTTTTTTCCTTATTGTATATAAAGAACTTGTGACTTTTAATATTAGCATTTTTGCTT
  TCAACAGCAATTAGCAATCTCTTGGTTTGCTGTTTGGTAAAGCATCGGTTAATGAGGTCA
  TCTAGTTTAAAATCCCAGCTACTTGAAAATAACAGGGAGGAGGTCAAATCTATCATTTCA
  TGGTATATTCTCTGCTCTCTTTTCAGTTCTCTAAAGAAAAGATATCTTTGTTATCCCACA
  TAAGTCATTACGACCCCGTACTGGCCCTTTGGTGTTTACTACCACCTCAAGATCACGTAG
  GTCCCTTTTTGCTTGAGTCCTGGAATTGGTAATTCTTCTTTTTACCACCACCCCAAAAAC
  AACTTTAAACATCTAAGGATGAGGAATGGTTTCCCGTGGTGGGGTTGGCCTTCCTCCACA
  GTTCTAGATTCACGTTATCCAGGGGTTGTCTTTTGTGGGAAAGGGGTCCCCGGGGGGGGT
  ACCCTCCGAGGGGTTCTGGGGGGGGGCCCCCCGCCCCCCACCCCCTTTTTTGCCGTACAA
  AAAACCCCCCTCGGGGTTTTCTCCTTTTTTGTGCCCCCCGAGGTCTTCCCCAAATAGAAG
  GGCCTCTTCACAACTTTGCCTGGCAAATTGACCCAAAATTTGGCTTGTTTCCACCCCCTG
  AGGTTTACTTCTTTTTAGGCCATTTCCACACAACCCCCTTGAACCCCCCGGTAAGGAAAA
  ATGGAAGGGCCCTCGCCCCTTGCCCTTGCCCTGGACCCGCGCCCCATTTGGCCCTTTTTT
  GGAAAATGGGGGGGTTTTCCTCCCCTAAAACGCCTTTGCACTCCTTTTCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004349
• Insert Size: 2800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004349.2, NP_004340.1
• RefSeq Size: 7420 bp
• RefSeq ORF: 1734 bp
• Locus ID: 862
• Cytogenetics: 8q21.3
• Domains: zf-MYND, TAFH
• Protein Families: Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer
• Gene Summary: 'This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]'
  Transcript Variant: This variant (1) encodes isoform A. Variants 1, 5 and 6 all encode isoform A, also known as MTG8a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.