PGP9.5 (UCHL1) (NM_004181) Human Untagged Clone

CAT#: SC117505

UCHL1 (untagged)-Human ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) (UCHL1)


  "NM_004181" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol UCHL1
Synonyms HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; SPG79; Uch-L1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC117505 sequence for NM_004181 edited (data generated by NextGen Sequencing)
ATGCAGCTCAAGCCGATGGAGATCAACCCCGAGATGCTGAACAAAGTGCTGTCCCGGCTG
GGGGTCGCCGGCCAGTGGCGCTTCGTGGACGTGCTGGGGCTGGAAGAGGAGTCTCTGGGC
TCGGTGCCAGCGCCTGCCTGCGCGCTGCTGCTGCTGTTTCCCCTCACGGCCCAGCATGAG
AACTTCAGGAAAAAGCAGATTGAAGAGCTGAAGGGACAAGAAGTTAGTCCTAAAGTGTAC
TTCATGAAGCAGACCATTGGGAATTCCTGTGGCACAATCGGACTTATTCACGCAGTGGCC
AATAATCAAGACAAACTGGGATTTGAGGATGGATCAGTTCTGAAACAGTTTCTTTCTGAA
ACAGAGAAAATGTCCCCTGAAGACAGAGCAAAATGCTTTGAAAAGAATGAGGCCATACAG
GCAGCCCATGATGCCGTGGCACAGGAAGGCCAATGTCGGGTAGATGACAAGGTGAATTTC
CATTTTATTCTGTTTAACAACGTGGATGGCCACCTCTATGAACTTGATGGACGAATGCCT
TTTCCGGTGAACCATGGCGCCAGTTCAGAGGACACCCTGCTGAAGGACGCTGCCAAGGTC
TGCAGAGAATTCACCGAGCGTGAGCAAGGAGAAGTCCGCTTCTCTGCCGTGGCTCTCTGC
AAGGCAGCCTAA

Clone variation with respect to NM_004181.4
>OriGene 5' read for NM_004181 unedited
ACAGTCAGATGTGTATCCGACTCATATGGGCGGCCGCGCAATCGGCACGAGGCCTCGTGC
CGAATTCGGCACGAGGCTTCCCTAGGCTATTTCTGCCGGGCGCTCCGCGAAGATGCAGCT
CAAGCCGATGGAGATCAACCCCGAGATGCTGAACAAAGTGCTGTCCCGGCTGGGGGTCGC
CGGCCAGTGGCGCTTCGTGGACGTGCTGGGGCTGGAAGAGGAGTCTCTGGGCTCGGTGCC
AGCGCCTGCCTGCGCGCTGCTGCTGCTGTTTCCCCTCACGGCCCAGCATGAGAACTTCAG
GAAAAAGCAGATTGAAGAGCTGAAGGGACAAGAAGTTAGTCCTAAAGTGTACTTCATGAA
GCAGACCATTGGGAATTCCTGTGGCACAATCGGACTTATTCACGCAGTGGCCAATAATCA
AGACAAACTGGGATTTGAGGATGGATCAGTTCTGAAACAGTTTCTTTCTGAAACAGAGAA
AATGTCCCCTGAAGACAGAGCAAAATGCTTTGAAAAGAATGAGGCCATACAGGCAGCCCA
TGATGCCGTGGCACAGGAAGGCCAATGTCGGGTAGATGACAAGGTGAATTTCCATTTTAT
TCTGTTTAACAACGTGGATGGCCACCTCTATGAACTTGATGGACGAATGCCTTTTCCGGT
GAACCATGGCGCCAGTTCAGAGGACACCCTGCTGCAGGACGCTGCCAAGGTCTGCAGAGA
ATTCACCGAGCGTGAGCAAGGAGACAGTCGCTTCTCTGCCGTGGCTCTCTGCAGGCAGCC
TAATGCTCTGTGGGAGGGACTTTGCTGAGTTCCCCTCTTCCATTCACATGAAAATATATA
CCCCCCCATGCAGTCTAGAATGCTTCAGTACTTGTGAAACACAGCTGATCTTCTGATCTG
CAGACACGCATTCC
>OriGene 3' read for NM_004181 unedited
AGGAGAGGCACTGGGGAGGGGTACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA
TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCAAACAACAAAAAAGTTTTTTTCCTTTT
GAGGACAAACAATTAAACCCCCTCCAAGGTTTTAACTTACAAACAAAAACCAAAGTTCCC
CTTTAAAGCGTTAAATATCGGATACAAAACATACCCTGGGGAAAATGCTTCACCATTTGA
AGCTCACACCACAATGGCCCAGGGGACAACTGGGCCCTCTGCTTGGGCTTAAATGCCTGG
GTGGGGCTAAGGGGAAAGCCTTTCTACAAAACAAAAAAACAGCTGGGTTTCACAAGTACT
GAAGCATTTTAAACTGGATGGGGGGGTATATATTTTCATGTTGAAGGGAAGAGGGGAAAT
CACCAAAGTCCCTCCCACAGAGCATTAGGCTGCCTTGCAAAGAGCCACGGNAAAGAAGCG
GACTTCTCCTTGCTCACGCTCGGGGAATTCTCTGAAAACTTGGNAGGGTTCTTAACAAGG
TGTCCTCTGACTGGCCCATGGTTCACCGGAAAAGCATTCGCCCATCAGTTCATAAAGGGG
CCATCCCCGTTGTTACAAATAAAATGAAATTCCCCTGTTTTTACCCAAATTGCCTTCTGT
GCCACGGATATGGGCCTGCTGATGCCTATTCTTTAAAACATTTGCTTGGCTCAGGGATTT
TCTTTGTTAAAAAAACTGTTAAAACGACCTCCTAAACCCATTTGTTTGATTTGCCACGGG
AAAAACCCATGGCCCAGATTCCAAGGGCGCTTAAGAAACCTAAGACAACTTTGCCCTACT
TTATTGCTTTCCGAACCCAGCGGCCGGAGGAAAAAAAAAGGCAGGGGGTTGGCAACCAAA
TTTTCACCCAATTCAAGCCTGGCGACCACGAAATTTTT
Restriction Sites NotI-NotI     
ACCN NM_004181
Insert Size 1180 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004181.3, NP_004172.2
RefSeq Size 1110 bp
RefSeq ORF 672 bp
Locus ID 7345
Cytogenetics 4p13
Domains Peptidase_C12
Protein Families Druggable Genome, Protease
Protein Pathways Parkinson's disease
Gene Summary 'The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]'

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