PGP9.5 (UCHL1) (NM_004181) Human Untagged Clone

CAT#: SC117505

UCHL1 (untagged)-Human ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) (UCHL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UCHL1
• Synonyms: HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; SPG79; Uch-L1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117505 sequence for NM_004181 edited (data generated by NextGen Sequencing)
  ATGCAGCTCAAGCCGATGGAGATCAACCCCGAGATGCTGAACAAAGTGCTGTCCCGGCTG
  GGGGTCGCCGGCCAGTGGCGCTTCGTGGACGTGCTGGGGCTGGAAGAGGAGTCTCTGGGC
  TCGGTGCCAGCGCCTGCCTGCGCGCTGCTGCTGCTGTTTCCCCTCACGGCCCAGCATGAG
  AACTTCAGGAAAAAGCAGATTGAAGAGCTGAAGGGACAAGAAGTTAGTCCTAAAGTGTAC
  TTCATGAAGCAGACCATTGGGAATTCCTGTGGCACAATCGGACTTATTCACGCAGTGGCC
  AATAATCAAGACAAACTGGGATTTGAGGATGGATCAGTTCTGAAACAGTTTCTTTCTGAA
  ACAGAGAAAATGTCCCCTGAAGACAGAGCAAAATGCTTTGAAAAGAATGAGGCCATACAG
  GCAGCCCATGATGCCGTGGCACAGGAAGGCCAATGTCGGGTAGATGACAAGGTGAATTTC
  CATTTTATTCTGTTTAACAACGTGGATGGCCACCTCTATGAACTTGATGGACGAATGCCT
  TTTCCGGTGAACCATGGCGCCAGTTCAGAGGACACCCTGCTGAAGGACGCTGCCAAGGTC
  TGCAGAGAATTCACCGAGCGTGAGCAAGGAGAAGTCCGCTTCTCTGCCGTGGCTCTCTGC
  AAGGCAGCCTAA
  
  Clone variation with respect to NM_004181.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004181 unedited
  ACAGTCAGATGTGTATCCGACTCATATGGGCGGCCGCGCAATCGGCACGAGGCCTCGTGC
  CGAATTCGGCACGAGGCTTCCCTAGGCTATTTCTGCCGGGCGCTCCGCGAAGATGCAGCT
  CAAGCCGATGGAGATCAACCCCGAGATGCTGAACAAAGTGCTGTCCCGGCTGGGGGTCGC
  CGGCCAGTGGCGCTTCGTGGACGTGCTGGGGCTGGAAGAGGAGTCTCTGGGCTCGGTGCC
  AGCGCCTGCCTGCGCGCTGCTGCTGCTGTTTCCCCTCACGGCCCAGCATGAGAACTTCAG
  GAAAAAGCAGATTGAAGAGCTGAAGGGACAAGAAGTTAGTCCTAAAGTGTACTTCATGAA
  GCAGACCATTGGGAATTCCTGTGGCACAATCGGACTTATTCACGCAGTGGCCAATAATCA
  AGACAAACTGGGATTTGAGGATGGATCAGTTCTGAAACAGTTTCTTTCTGAAACAGAGAA
  AATGTCCCCTGAAGACAGAGCAAAATGCTTTGAAAAGAATGAGGCCATACAGGCAGCCCA
  TGATGCCGTGGCACAGGAAGGCCAATGTCGGGTAGATGACAAGGTGAATTTCCATTTTAT
  TCTGTTTAACAACGTGGATGGCCACCTCTATGAACTTGATGGACGAATGCCTTTTCCGGT
  GAACCATGGCGCCAGTTCAGAGGACACCCTGCTGCAGGACGCTGCCAAGGTCTGCAGAGA
  ATTCACCGAGCGTGAGCAAGGAGACAGTCGCTTCTCTGCCGTGGCTCTCTGCAGGCAGCC
  TAATGCTCTGTGGGAGGGACTTTGCTGAGTTCCCCTCTTCCATTCACATGAAAATATATA
  CCCCCCCATGCAGTCTAGAATGCTTCAGTACTTGTGAAACACAGCTGATCTTCTGATCTG
  CAGACACGCATTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004181 unedited
  AGGAGAGGCACTGGGGAGGGGTACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCAAACAACAAAAAAGTTTTTTTCCTTTT
  GAGGACAAACAATTAAACCCCCTCCAAGGTTTTAACTTACAAACAAAAACCAAAGTTCCC
  CTTTAAAGCGTTAAATATCGGATACAAAACATACCCTGGGGAAAATGCTTCACCATTTGA
  AGCTCACACCACAATGGCCCAGGGGACAACTGGGCCCTCTGCTTGGGCTTAAATGCCTGG
  GTGGGGCTAAGGGGAAAGCCTTTCTACAAAACAAAAAAACAGCTGGGTTTCACAAGTACT
  GAAGCATTTTAAACTGGATGGGGGGGTATATATTTTCATGTTGAAGGGAAGAGGGGAAAT
  CACCAAAGTCCCTCCCACAGAGCATTAGGCTGCCTTGCAAAGAGCCACGGNAAAGAAGCG
  GACTTCTCCTTGCTCACGCTCGGGGAATTCTCTGAAAACTTGGNAGGGTTCTTAACAAGG
  TGTCCTCTGACTGGCCCATGGTTCACCGGAAAAGCATTCGCCCATCAGTTCATAAAGGGG
  CCATCCCCGTTGTTACAAATAAAATGAAATTCCCCTGTTTTTACCCAAATTGCCTTCTGT
  GCCACGGATATGGGCCTGCTGATGCCTATTCTTTAAAACATTTGCTTGGCTCAGGGATTT
  TCTTTGTTAAAAAAACTGTTAAAACGACCTCCTAAACCCATTTGTTTGATTTGCCACGGG
  AAAAACCCATGGCCCAGATTCCAAGGGCGCTTAAGAAACCTAAGACAACTTTGCCCTACT
  TTATTGCTTTCCGAACCCAGCGGCCGGAGGAAAAAAAAAGGCAGGGGGTTGGCAACCAAA
  TTTTCACCCAATTCAAGCCTGGCGACCACGAAATTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004181
• Insert Size: 1180 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004181.3, NP_004172.2
• RefSeq Size: 1110 bp
• RefSeq ORF: 672 bp
• Locus ID: 7345
• Cytogenetics: 4p13
• Domains: Peptidase_C12
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Parkinson's disease
• Gene Summary: 'The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]'