KCNMB1 (NM_004137) Human Untagged Clone

CAT#: SC117547

KCNMB1 (untagged)-Human potassium large conductance calcium-activated channel, subfamily M, beta member 1 (KCNMB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNMB1
• Synonyms: BKbeta1; hbeta1; hslo-beta; K(VCA)beta; k(VCA)beta-1; SLO-BETA; slo-beta-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117547 sequence for NM_004137 edited (data generated by NextGen Sequencing)
  ATGGTGAAGAAGCTGGTGATGGCCCAGAAGCGGGGAGAGACACGAGCCCTTTGCCTGGGT
  GTAACCATGGTGGTGTGTGCCGTCATCACCTACTACATCCTGGTCACGACTGTGCTGCCC
  CTCTACCAGAAAAGCGTGTGGACCCAGGAATCCAAGTGCCACCTGATTGAGACCAACATC
  AGGGACCAGGAGGAGCTGAAGGGCAAGAAGGTGCCCCAGTACCCATGCCTGTGGGTCAAC
  GTGTCAGCTGCCGGCAGGTGGGCTGTGCTGTACCACACGGAGGACACTCGGGACCAGAAC
  CAGCAGTGCTCCTACATCCCAGGCAGCGTGGACAATTACCAGACGGCCCGGGCCGACGTG
  GAGAAGGTCAGAGCCAAATTCCAAGAGCAGCAGGTCTTCTACTGCTTCTCCGCACCTCGG
  GGGAACGAAACCAGCGTCCTATTCCAGCGCCTCTACGGGCCCCAGGCCCTCCTCTTCTCC
  CTCTTCTGGCCCACCTTCCTGCTGACCGGTGGCCTCCTCATTATCGCCATGGTGAAGAGC
  AACCAGTACCTGTCCATCCTGGCGGCCCAGAAGTAG
  
  Clone variation with respect to NM_004137.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004137 unedited
  CTGGATTTGTAACGACTTATATAGGCGGCAGCGCAATTCGCACGAGGGAAGAAATATTTC
  CAAATATACCAGGCTGATCTTTCGTAGTTTCTCAGTCTTGCTGTCTGTTGTCGTTTGAAA
  TCTGTTTCCCACGAGACTCTGGGAAGATGGAGGTGCAGGGCTTGGGTAACAGCTGCCTTC
  CTCAGGGCTGGGTACCTGCTCTCCGAGTCTGTGTCTCCAGGCTGTGTGCTCAGACTTGAC
  CATGTTTGGGCATGGAGGCTTGCCCCAGATGGGGACCAAAAGCTGCTTCCCTGGAGACGG
  GCGGGGAGGCCAAACTTCCTGCTGAAGCTCTGTGGCCTCTTTTGGGGTGGGGGCGGGGGT
  CCAGGCAGAAAGAAACCGTCTGCTGCTCAAGACCCACAGGACGCCGGGAAGACTAAATGA
  TCACTGCCCCCAGTGAATATGGTGAAGAAGCTGGTGATGGCCCAGAAGCGGGGAGAGACA
  CGAGCCCTTTGCCTGGGTGTAACCATGGTGGTGTGTGCCGTCATCACCTACTACATCCTG
  GTCACGACTGTGCTGCCCCTCTACCAGAAAAGCGTGTGGACCCAGGAATCCAAGTGCCAC
  CTGATTGAGACCAACATCANGGACCAGGAGGAGCTGAAGGGCAAGAAGGTGCCCCAGTAC
  CCATGCCTGTGGGTCAACGTGTCAGCTGCCGGCAGGTGGGCTGTGCTGTACCACACGGAG
  GACACTCGGGACCAGAACCAGCAGTGCTCCTACATCCNAGCAGCGTGGACATTACCAGAC
  GCCCGGGCCGACGTGNAGAAGTCAGAGCAATTNCCAAGACAGCAGGTCTTCTACTGCTTC
  TCCGGCACTCGGGGGGACGAAACCAGCGTCCTTATCCAGCGCCTCTACGGGCCCCAAGCC
  CTNCTCTTCTCCCTCTTCTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004137 unedited
  NNTTTTCACTTTGGACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTGT
  TGGTTGGCATACATTTATTAATATTGGAATTAAAAATTATATGGCACTTTATATCCTAGA
  AAATAGTAATACTGTAAATGTGTTCTAGAAATGGGAGCTGCTGTTGCTCTTATTAGAGAA
  TTCAAACAAAGAAGGGAGGCTCGCTGGGGACAGCTTCTGGGGGAGGATGGGTACCGCTTT
  GAGACAACAGGGAGAACTCAGGCACAGAGGTGACGATCATCGTTCTCTGTGGGGCACATA
  GTGATGCAGCCGGAAACAGGTATGAGTCAGTTGAGTGGGGACAGGTAATAGAGAGCTAGA
  ACTGGCTGGCCTTATGGCCTCCAAGGCATTGGGGAGCCACTGTACATTCTTGAGCAGGCA
  ATGACTTCACAAAAGGATTTCTCAAAGGTTAGTCCTGCAACAGAAGACAGCGTGGATTGG
  ACTGGAAGAGTGGGAGGGCAGGTGGAGAAGGCATTGTGCTGCAAGTGGGGAGCAGCCCTG
  GGGGCCCAGCCAGTCCCCTGTGCCCTGACAAGTGGTATGGCATGGATGGATGGCTCTACT
  TCTGGGCCGCCAGGATGGACAGGTACTGGTTGCTCTTCACCATGGCGATAATGAGGAGGC
  CACCGGTCAGCAGGAAGGTGGGCCAGAAGAGGGAGAAGAGGAGGGCCTGGGGCCCGTANA
  GGCGCTGGAATAGGACGCTGGTTTCGTTCCCCCGAGGTGCGGAGAAGCAGTAGAAGACCT
  GCTGCTCTTGGAAATTTGCTCTGACCTTCTCCACGTCGGCCCGGGCCGTCTGTTATTGTC
  CACGCTGCCTGGGATGTANGAGCACTGCTGGNTCTGTCCCCGAGTGCCTCCGTGTGTACA
  G
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004137
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004137.2, NP_004128.1
• RefSeq Size: 1518 bp
• RefSeq ORF: 576 bp
• Locus ID: 3779
• Cytogenetics: 5q35.1
• Domains: CaKB
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Protein Pathways: Vascular smooth muscle contraction
• Gene Summary: 'MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the product of this gene, the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. [provided by RefSeq, Jul 2008]'