PP2A-beta (PPP2CB) (NM_004156) Human Untagged Clone

CAT#: SC117555

PPP2CB (untagged)-Human protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform (PPP2CB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2CB
• Synonyms: AI115466; D8Ertd766e; PP2Ac; PP2CB; protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform; protein phosphatase 2a, catalytic subunit, beta isoform
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004156, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACGACAAGGCGTTCACCAAGGAGCTGGACCAGTGGGTCGAGCAGCTGAACGAGTGTAAGCAGCTGA
  ACGAGAACCAAGTGCGGACGCTGTGCGAGAAGGCAAAGGAAATTTTAACAAAAGAATCAAATGTGCAAGA
  GGTTCGTTGCCCTGTTACTGTCTGTGGAGATGTGCATGGTCAATTTCATGATCTTATGGAACTCTTTAGA
  ATTGGTGGAAAATCACCGGATACAAACTACTTATTCATGGGTGACTATGTAGACAGAGGATATTATTCAG
  TGGAGACTGTGACTCTTCTTGTAGCATTAAAGGTGCGTTATCCAGAACGCATTACAATATTGAGAGGAAA
  TCACGAAAGCCGACAAATTACCCAAGTATATGGCTTTTATGATGAATGTCTGCGAAAGTATGGGAATGCC
  AACGTTTGGAAATATTTTACAGATCTCTTTGATTATCTTCCACTTACAGCTTTAGTAGATGGACAGATAT
  TCTGCCTCCATGGTGGCCTCTCTCCATCCATAGACACACTGGATCATATAAGAGCCCTGGATCGTTTACA
  GGAAGTTCCACATGAGGGCCCAATGTGTGATCTGTTATGGTCAGATCCAGATGATCGTGGTGGATGGGGT
  ATTTCACCACGTGGTGCTGGCTACACATTTGGACAAGACATTTCTGAAACCTTTAACCATGCCAATGGTC
  TCACACTGGTTTCTCGTGCCCACCAGCTTGTAATGGAGGGATACAATTGGTGTCATGATCGGAATGTGGT
  TACCATTTTCAGTGCACCCAATTACTGTTATCGTTGTGGGAACCAGGCTGCTATCATGGAATTAGATGAC
  ACTTTAAAATATTCCTTCCTTCAATTTGACCCAGCGCCTCGTCGTGGTGAGCCTCATGTTACACGGCGCA
  CCCCAGACTACTTCCTATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004156 unedited
  GGTCAATATTGTATACGACTCCTAAAGGCGGCCGCGACATCGGCACGATGCGGCGGCCGG
  GCTTGGGCTCGGGATCCGCATCGGGATCGGGCCGCCATGGACGACAAGGCGTTCACCAAG
  GAGCTGGACCAGTGGGTCGAGCAGCTGAACGAGTGTAAGCAGCTGAACGAGAACCAAGTG
  CGGACGCTGTGCGAGAAGGCAAAGGAAATTTTAACAAAAGAATCAAATGTGCAAGAGGTT
  CGTTGCCCTGTTACTGTCTGTGGAGAATGTGCATGGTCAATTTCATGATCTTATGGAACT
  CTTTAAAATTGGTGGAAAATCACCGGAAACAAACTTACTTATTCATGGGTGACTATGTAG
  ACAGAGGATATTATTCAGTGGAGACTGTGACTCTTCTTGTAGCATTAAAGGTGCGTTATC
  CAGAACGCATTACAATATTGAGAGGAAATCACGAAAGCCGACAAATTACCCAAGTATATG
  GCTTTTAATGAATAAATGTCTGCGAAAGTATGGGAATGCCAACGTTTGGAAATATTTTAC
  AGAATCTCTTTGATTATCTTCCACTTACAGCTTTAGTAGAATGGACAGATATTCTGCCTC
  CATGGTGGCCTCTCTTCATTCATTAGACACACTGGATATATAAAACCCTGGATCGTTACA
  GGAAGTTCCACATGAGGCCAATGTGTGATCTGTTATGGTCAAATCAGAATGATCGTGGTG
  ATGGNNGTATTTCACCCACGGGNTGCTGGCTACACATTNTGACAGACATTTCTGGAACCT
  TTTAACCATGCAATGGTCTCACACTGTTTNCTCGTGCCCACCAGCTTGTATGGNAGGATA
  CAATTGNGGNCATATCNGGAAAGGNGTTACATTTTTNANGGCACCAATTACTGGTTTTNG
  TTGTGGAAACCAGCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004156 unedited
  NATGGCAACTTCCAGGTCCAGAAGAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA
  TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTT
  TTTTGTTTAAAATGAAAAGTTTATTTGCTGAGTACACCAAATAGGATGCAAGCACTGTCA
  TGACAAATATACAGAAATATGCAGATCAACATAAAACAGTAATTTAGTTTAAATGAAGGG
  AAATCTCTTTAAATGTTATGACAACATACTCCCAAGAAGAACCTTTTCTTCAGTTAAGTT
  AATTATACATTTCAATAAAATAAAGGATAATGGATTAGTGGAAGTTAGCTTGTGAATTTT
  TCTTAAAAATAAAACTCCAACTCTATTAATCCATGCCAGTTAAACACTATAACTAAAATT
  TCCAAATAAGCGCAAAAGGAGATGAAGCAGTTAGTTACCTTTTTTGCTTGAACAGTCCAA
  AGGAAAATGGTTACTATAAATACAGCAGGCAAACTGTTAGACTGACCTAGAACATAGTGT
  ACTAAATTTCAGTCTCAAATTGTGCTAAATGCTCATCATTAGTATGGCACATTTGGTCCA
  TGATGTGGTTTAATGCCAAGACAGATCCCAATTTGTTACAGAAACCATAGATTACTGGTG
  CTTTTTGTTTTTAATACATATATTTTAAAAGCCAGGTATACTTCCACATACAAGGCNNAG
  TTTCCAGNAGAATTATANCGAAGTACTCTGGGTGGCGCGTGTACATGAGGCTCACACGAC
  GAGCGCTGGGTCAATGAAGGAGGATTTTTAAGTGGTATTATTCTGAAGAAACTGGTCACA
  CGAAAGAATTGGTGCCTGAAAGTACATTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004156
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004156.2, NP_004147.1
• RefSeq Size: 1807 bp
• RefSeq ORF: 930 bp
• Locus ID: 5516
• Cytogenetics: 8p12
• Domains: Metallophos, PP2Ac
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
• Gene Summary: 'This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. [provided by RefSeq, Mar 2010]'
  Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein.