PP2A-beta (PPP2CB) (NM_004156) Human Untagged Clone

CAT#: SC117555

PPP2CB (untagged)-Human protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform (PPP2CB), transcript variant 1


  "NM_004156" in other vectors (4)

Reconstitution Protocol

USD 660.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PPP2CB
Synonyms AI115466; D8Ertd766e; PP2Ac; PP2CB; protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform; protein phosphatase 2a, catalytic subunit, beta isoform
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_004156, the custom clone sequence may differ by one or more nucleotides


ATGGACGACAAGGCGTTCACCAAGGAGCTGGACCAGTGGGTCGAGCAGCTGAACGAGTGTAAGCAGCTGA
ACGAGAACCAAGTGCGGACGCTGTGCGAGAAGGCAAAGGAAATTTTAACAAAAGAATCAAATGTGCAAGA
GGTTCGTTGCCCTGTTACTGTCTGTGGAGATGTGCATGGTCAATTTCATGATCTTATGGAACTCTTTAGA
ATTGGTGGAAAATCACCGGATACAAACTACTTATTCATGGGTGACTATGTAGACAGAGGATATTATTCAG
TGGAGACTGTGACTCTTCTTGTAGCATTAAAGGTGCGTTATCCAGAACGCATTACAATATTGAGAGGAAA
TCACGAAAGCCGACAAATTACCCAAGTATATGGCTTTTATGATGAATGTCTGCGAAAGTATGGGAATGCC
AACGTTTGGAAATATTTTACAGATCTCTTTGATTATCTTCCACTTACAGCTTTAGTAGATGGACAGATAT
TCTGCCTCCATGGTGGCCTCTCTCCATCCATAGACACACTGGATCATATAAGAGCCCTGGATCGTTTACA
GGAAGTTCCACATGAGGGCCCAATGTGTGATCTGTTATGGTCAGATCCAGATGATCGTGGTGGATGGGGT
ATTTCACCACGTGGTGCTGGCTACACATTTGGACAAGACATTTCTGAAACCTTTAACCATGCCAATGGTC
TCACACTGGTTTCTCGTGCCCACCAGCTTGTAATGGAGGGATACAATTGGTGTCATGATCGGAATGTGGT
TACCATTTTCAGTGCACCCAATTACTGTTATCGTTGTGGGAACCAGGCTGCTATCATGGAATTAGATGAC
ACTTTAAAATATTCCTTCCTTCAATTTGACCCAGCGCCTCGTCGTGGTGAGCCTCATGTTACACGGCGCA
CCCCAGACTACTTCCTATAA


>OriGene 5' read for NM_004156 unedited
GGTCAATATTGTATACGACTCCTAAAGGCGGCCGCGACATCGGCACGATGCGGCGGCCGG
GCTTGGGCTCGGGATCCGCATCGGGATCGGGCCGCCATGGACGACAAGGCGTTCACCAAG
GAGCTGGACCAGTGGGTCGAGCAGCTGAACGAGTGTAAGCAGCTGAACGAGAACCAAGTG
CGGACGCTGTGCGAGAAGGCAAAGGAAATTTTAACAAAAGAATCAAATGTGCAAGAGGTT
CGTTGCCCTGTTACTGTCTGTGGAGAATGTGCATGGTCAATTTCATGATCTTATGGAACT
CTTTAAAATTGGTGGAAAATCACCGGAAACAAACTTACTTATTCATGGGTGACTATGTAG
ACAGAGGATATTATTCAGTGGAGACTGTGACTCTTCTTGTAGCATTAAAGGTGCGTTATC
CAGAACGCATTACAATATTGAGAGGAAATCACGAAAGCCGACAAATTACCCAAGTATATG
GCTTTTAATGAATAAATGTCTGCGAAAGTATGGGAATGCCAACGTTTGGAAATATTTTAC
AGAATCTCTTTGATTATCTTCCACTTACAGCTTTAGTAGAATGGACAGATATTCTGCCTC
CATGGTGGCCTCTCTTCATTCATTAGACACACTGGATATATAAAACCCTGGATCGTTACA
GGAAGTTCCACATGAGGCCAATGTGTGATCTGTTATGGTCAAATCAGAATGATCGTGGTG
ATGGNNGTATTTCACCCACGGGNTGCTGGCTACACATTNTGACAGACATTTCTGGAACCT
TTTAACCATGCAATGGTCTCACACTGTTTNCTCGTGCCCACCAGCTTGTATGGNAGGATA
CAATTGNGGNCATATCNGGAAAGGNGTTACATTTTTNANGGCACCAATTACTGGTTTTNG
TTGTGGAAACCAGCTT
>OriGene 3' read for NM_004156 unedited
NATGGCAACTTCCAGGTCCAGAAGAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA
TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTT
TTTTGTTTAAAATGAAAAGTTTATTTGCTGAGTACACCAAATAGGATGCAAGCACTGTCA
TGACAAATATACAGAAATATGCAGATCAACATAAAACAGTAATTTAGTTTAAATGAAGGG
AAATCTCTTTAAATGTTATGACAACATACTCCCAAGAAGAACCTTTTCTTCAGTTAAGTT
AATTATACATTTCAATAAAATAAAGGATAATGGATTAGTGGAAGTTAGCTTGTGAATTTT
TCTTAAAAATAAAACTCCAACTCTATTAATCCATGCCAGTTAAACACTATAACTAAAATT
TCCAAATAAGCGCAAAAGGAGATGAAGCAGTTAGTTACCTTTTTTGCTTGAACAGTCCAA
AGGAAAATGGTTACTATAAATACAGCAGGCAAACTGTTAGACTGACCTAGAACATAGTGT
ACTAAATTTCAGTCTCAAATTGTGCTAAATGCTCATCATTAGTATGGCACATTTGGTCCA
TGATGTGGTTTAATGCCAAGACAGATCCCAATTTGTTACAGAAACCATAGATTACTGGTG
CTTTTTGTTTTTAATACATATATTTTAAAAGCCAGGTATACTTCCACATACAAGGCNNAG
TTTCCAGNAGAATTATANCGAAGTACTCTGGGTGGCGCGTGTACATGAGGCTCACACGAC
GAGCGCTGGGTCAATGAAGGAGGATTTTTAAGTGGTATTATTCTGAAGAAACTGGTCACA
CGAAAGAATTGGTGCCTGAAAGTACATTC
Restriction Sites NotI-NotI     
ACCN NM_004156
Insert Size 1600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004156.2, NP_004147.1
RefSeq Size 1807 bp
RefSeq ORF 930 bp
Locus ID 5516
Cytogenetics 8p12
Domains Metallophos, PP2Ac
Protein Families Druggable Genome, Phosphatase
Protein Pathways Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
Gene Summary 'This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. [provided by RefSeq, Mar 2010]'
Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein.

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