Rab5 (RAB5A) (NM_004162) Human Untagged Clone

CAT#: SC117557

RAB5A (untagged)-Human RAB5A, member RAS oncogene family (RAB5A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAB5A
• Synonyms: RAB5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117557 sequence for NM_004162 edited (data generated by NextGen Sequencing)
  ATGGCTAGTCGAGGCGCAACAAGACCCAACGGGCCAAATACTGGAAATAAAATATGCCAG
  TTCAAACTAGTACTTCTGGGAGAGTCCGCTGTTGGCAAATCAAGCCTAGTGCTTCGTTTT
  GTGAAAGGCCAATTTCATGAATTTCAAGAGAGTACCATTGGGGCTGCTTTTCTAACCCAA
  ACTGTATGTCTTGATGACACTACAGTAAAGTTTGAAATATGGGATACAGCTGGTCAAGAA
  CGATACCATAGCCTAGCACCAATGTACTACAGAGGAGCACAAGCAGCCATAGTTGTATAT
  GATATCACAAATGAGGAGTCCTTTGCAAGAGCAAAAAATTGGGTTAAAGAACTTCAGAGG
  CAAGCAAGTCCTAACATTGTAATAGCTTTATCGGGAAACAAGGCCGACCTAGCAAATAAA
  AGAGCAGTAGATTTCCAGGAAGCACAGTCCTATGCAGATGACAATAGTTTATTATTCATG
  GAGACATCCGCTAAAACATCAATGAATGTAAATGAAATATTCATGGCAATAGCTAAAAAA
  TTGCCAAAGAATGAACCACAAAATCCAGGAGCAAATTCTGCCAGAGGAAGAGGAGTAGAC
  CTTACCGAACCCACACAACCAACCAGGAATCAGTGTTGTAGTAACTAA
  
  Clone variation with respect to NM_004162.4
  42 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004162 unedited
  TTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGCTAAGAGCAGGCGA
  CGCCGCCGCCGCCACCACCACCGCCATAGATACACTCTCATCCTACGGGCCACGCCTGGG
  CCTTGCTGCCAGGAAGCTTCGGCCCCGCAGCTCGGCTTGCTGCGGTCTCAGGTTTCTTTA
  CCTCCAGAAAGAAGAATATTGGCCCCTTGAATTCTGGAAGTTCATTGAAGAGTCTGAAAT
  TAGGGACTTATTTCAAATTTGGACATGGCTAGTCGAGGCGCAACAAGACCCAACGGGCCA
  AATACTGGAAATAAAATATGCCAGTTCAAACTAGTACTTCTGGGAGAGTCCGCTGTTGGC
  AAATCAAGCCTAGTGCTTCGTTTTGTGAAAGGCCAATTTCATGAATTTCAAGAGAGTACC
  ATTGGGGCTGCTTTTCTAACCCAAACTGTATGTCTTGATGACACTACAGTAAAGTTTGAA
  ATATGGGATACAGCTGGTCAAGAACGATACCATAGCCTAGCACCAATGTACTACAGAGGA
  GCACAAGCAGCCATAGTTGTATATGATATCACAAATGAGGAGTCCTTTGCAAGAGCAAAA
  AATTGGGTTAAAGAACTTCAGAGGCAAGCAAGTCCTAACATTGTAATAGCTTTATCGGGA
  AACAAGGCCGACCTAGCAATAAAAGAGCAGTAGATTTCCAGGAAGCACAGTCCTATGCAG
  ATGACAATAGTTTATTATTCATGGAGACATCCGCTAAACATCAATGAATGTAAATGAAAT
  ATTCATGGGCATAGCNTANNAATTGCCAAAAGATGACCACANATCCAGNAGCANATTCTG
  CCAGAGGAGNAGGAGTAGACCTTACCGACCCCACNANCANCAGNNNATCATGGTGTAGTA
  ACTAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004162 unedited
  GCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCTTTTTATACCACTTTATTCC
  AACCTGAGCACCTCAATATAAAACTAAACACTGGTGAACCGTTTTCCTAATTCTGCATTA
  TCATAAATGTACAAGTTCTCCTAGCAGTCCAACACTTAAATAGATTAAATCATCTCTGAC
  ACATGGTAGCTTTCATATAATGAAAATACCTAAAATAATTAGTGCAATATACTGAACTGA
  TCAAAATAAAATGAACTTTGGAAAGGAAGGCTGCAAGATTGTTACTAACATATTCCAATA
  CTTTATGTTACAAATTACGGGTACATTGTTTATTATGGTTCTAGCCATGGAGGAACAACT
  GAAGCCCCTTCCTGTCAAAGGGGGAGAGAGAAATAATTAGCTGTTTTAGTAACAGTACAT
  TTTGTATGCTTTTAAGCAACTCTTAAATATTACAGAAAAGTAATACAGATATGGAGACTA
  CAATGCAGTACCCTATTTACAGTACAGCTGAAGATCGAATTTAAAATAATCAAGTTTGAA
  TATACATAATTGTTAGTGCATTGACTATATTATGTCAAAAGGGAACAAAAGCCCCCTCCC
  CACCCCAAACAACAAAACCTGTGTAATGGCCAGCGGTGATTAATTAGAAATCACAGACAC
  CTGGTTTATTTCATTTTTGTTAAAGAACTCACATGTTTGAAAGGAACACTGACCATTAAA
  GTGCATTNATTAGTTCTCAAAATTCCTAAAACAAGATGGGGTTGCATCTGATTTTTACCC
  CATTTAAAAAAAAAAACCAGACTGGGTATAATAAGATACCATTATACTCTTCCCACCCTG
  ACCTGTTATGTGGGATATTTTCTTGGGGTAATTATTAAATGTGGAGACATTTTATACAGT
  ACCAGAGAATGACATTGCTGNACTATGCTAGCATATAATACTTAAATCTCTCTAACAGAA
  GT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004162
• Insert Size: 2190 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004162.3, NP_004153.2
• RefSeq Size: 2352 bp
• RefSeq ORF: 648 bp
• Locus ID: 5868
• Cytogenetics: 3p24.3
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Endocytosis
• Gene Summary: ''
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).