RAB27B (NM_004163) Human Untagged Clone

CAT#: SC117558

RAB27B (untagged)-Human RAB27B, member RAS oncogene family (RAB27B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAB27B
• Synonyms: C25KG
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117558 sequence for NM_004163 edited (data generated by NextGen Sequencing)
  ATGACCGATGGAGACTATGATTATCTGATCAAACTCCTGGCCCTCGGGGATTCAGGGGTG
  GGGAAGACAACATTTCTTTATAGATACACAGATAATAAATTCAATCCCAAATTCATCACT
  ACAGTAGGAATAGACTTTCGGGAAAAACGTGTGGTTTATAATGCACAAGGACCGAATGGA
  TCTTCAGGGAAAGCATTTAAAGTGCATCTTCAGCTTTGGGACACTGCGGGACAAGAGCGG
  TTCCGGAGTCTCACCACTGCATTTTTCAGAGACGCCATGGGCTTCTTATTAATGTTTGAC
  CTCACCAGTCAACAGAGCTTCTTAAATGTCAGAAACTGGATGAGCCAACTGCAAGCAAAT
  GCTTATTGTGAAAATCCAGATATAGTATTAATTGGCAACAAGGCAGACCTACCAGATCAG
  AGGGAAGTCAATGAACGGCAAGCTCGGGAACTGGCTGACAAATATGGCATACCATATTTT
  GAAACAAGTGCAGCAACTGGACAGAATGTGGAGAAAGCTGTAGAAACCCTTTTGGACTTA
  ATCATGAAGCGAATGGAACAGTGTGTGGAGAAGACACAAATCCCTGATACTGTCAATGGT
  GGAAATTCTGGAAACTTGGATGGGGAAAAGCCACCAGAGAAGAAATGTATCTGCTAG
  
  Clone variation with respect to NM_004163.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004163 unedited
  NNNNTGGTCGAATTTGTATACGACTCATATAGGGCGGCCGCGAATCGGCACCAGGGGAAA
  CTTCGCAGGCTGACCGACCAAGACCATCACTATGACCGATGGAGACTATGATTATCTGAT
  CAAACTCCTGGCCCTCGGGGATTCAGGGGTGGGGAAGACAACATTTCTTTATAGATACAC
  AGATAATAAATTCAATCCCAAATTCATCACTACAGTAGGAATAGACTTTCGGGAAAAACG
  TGTGGTTTATAATGCACAAGGACCGAATGGATCTTCAGGGAAAGCATTTAAAGTGCATCT
  TCAGCTTTGGGACACTGCGGGACAAGAGCGGTTCCGGAGTCTCACCACTGCATTTTTCAG
  AGACGCCATGGGCTTCTTATTAATGTTTGACCTCACCAGTCAACAGAGCTTCTTAAATGT
  CAGAAACTGGATGAGCCAACTGCAAGCAAATGCTTATTGTGAAAATCCAGATATAGTATT
  AATTGGGCACAAGGCAGACCTACCAGATCAGAGGGAAGTCAATGAACGGGGCAGCTCGGA
  ACTGGCTGACAAATATGGCATACCATATTTTGAAACAAGTGCAGNCACTGGACAGAATGT
  GGAGAAAGCTGTAGAAACCTTTTGGNACTTATCATGAAGCGAATGGNACAGTGTGTGGAG
  AAGACACAAATCCCTGATACTGTCAATGGTGGNNAATCTGGNAACTGGNATGGGGGAAAG
  CCACCAGAGAAGAAATGTATCTGCTAGACTCTACATAGAAACTGGGACATCAGAACCCCN
  NACANNATATACTTTTANANAANCATGACAACCACACANTNGGTGNTGAGTAAACACGCA
  CATGGCATGTCTTTCTTTTCTGCCAGANATCTATTTAAGAAACAGATAGTCAACAGTGGT
  CAAAGAAATGAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004163
• Insert Size: 6450 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004163.3, NP_004154.2
• RefSeq Size: 1365 bp
• RefSeq ORF: 657 bp
• Locus ID: 5874
• Cytogenetics: 18q21.2
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Gene Summary: 'Members of the Rab protein family, including RAB27B, are prenylated, membrane-bound proteins involved in vesicular fusion and trafficking (Chen et al., 1997 [PubMed 9066979]).[supplied by OMIM, Nov 2010]'