BYSL (NM_004053) Human Untagged Clone

CAT#: SC117600

BYSL (untagged)-Human bystin-like (BYSL)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BYSL
• Synonyms: BYSTIN; Enp1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117600 sequence for NM_004053 edited (data generated by NextGen Sequencing)
  ATGCCCAAATTCAAGGCGGCCCGTGGGGTGGGGGGTCAGGAAAAACATGCGCCCCTGGCC
  GATCAGATCCTGGCTGGGAATGCGGTGCGGGCGGGGGTCCGGGAGAAGCGGCGGGGTCGC
  GGGACAGGAGAAGCGGAGGAAGAGTATGTGGGGCCCCGGCTGAGCCGACGGATTTTGCAG
  CAAGCACGGCAGCAACAGGAGGAACTCGAGGCCGAGCATGGGACTGGGGACAAGCCCGCG
  GCGCCGCGGGAACGCACCACGCGGCTGGGTCCAAGAATGCCTCAGGATGGATCAGATGAC
  GAGGACAAGGAGTGGCCCACCCTGGAGAAGGCTGCCACAATGACAGCAGCGGGCCATCAT
  GCAGAGGTGGTTGTGGACCCTGAGGATGAGCGTGCCATAGAGATGTTCATGAACAAGAAC
  CCTCCTGCCAGGCGCACCCTGGCTGACATCATCATGGAGAAGCTGACTGAGAAGCAGACA
  GAGGTTGAGACAGTCATGTCAGAGGTGTCGGGCTTCCCTATGCCCCAGCTGGACCCCCGG
  GTCCTAGAAGTGTACAGGGGGGTCCGGGAGGTATTATCTAAGTACCGCAGTGGAAAACTG
  CCCAAGGCATTTAAGATCATCCCTGCACTCTCCAACTGGGAGCAAATCCTCTACGTCACA
  GAGCCGGAGGCCTGGACTGCAGCTGCCATGTACCAGGCCACCAGGATTTTTGCCTCTAAC
  CTGAAGGAACGCATGGCCCAGCGCTTCTACAACCTTGTCCTGCTCCCTCGAGTACGAGAT
  GACGTTGCTGAATACAAACGACTCAACTTCCATCTCTACATGGCTCTCAAGAAGGCCCTT
  TTCAAACCTGGAGCCTGGTTCAAAGGGATCCTGATTCCACTGTGCGAGTCTGGCACTTGT
  ACCCTCCGGGAAGCCATCATTGTGGGTAGCATCATCACCAAGTGCTCCATCCCTGTGTTG
  CACTCCAGTGCGGCCATGCTGAAAATTGCTGAGATGGAATACAGCGGTGCCAACAGCATC
  TTCCTGCGACTGCTGCTGGATAAGAAGTATGCACTGCCTTACCGGGTGCTGGATGCCCTA
  GTCTTCCACTTCCTGGGGTTCCGGACAGAGAAGCGTGAACTGCCTGTGCTGTGGCACCAG
  TGCCTCCTGACTTTGGTCCAGCGCTACAAGGCCGACTTGGCCACAGACCAGAAAGAGGCC
  CTCTTAGAACTGCTCCGGCTGCAGCCCCATCCACAGCTATCGCCCGAAATCAGGCGTGAG
  CTTCAGAGTGCAGTCTCCCGCGATGTGGAAGATGTTCCCATCACCGTGGAGTGA
  
  Clone variation with respect to NM_004053.3
  307 g=>a;1276 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004053 unedited
  TTTTGTATACGACTACTATAGGCGGCCGCGAATTCGGCACGAGGCACGTGCGATCCTTCC
  CGGCAACTTTTTCGAGAAAAATGCCCAAATTCAAGGCGGCCCGTGGGGTGGGGGGTCAGG
  AAAAACATGCGCCCCTGGCCGATCAGATCCTGGCTGGGAATGCGGTGCGGGCGGGGGTCC
  GGGAGAAGCGGCGGGGTCGCGGGACAGGAGAAGCGGAGGAAGAGTATGTGGGGCCCCGGC
  TGAGCCGACGGATTTTGCAGCAAGCACGGCAGCAACAGGAGGAACTCGAGGCCGAGCATG
  GGACTGGGGACAAGCCCGCGGCGCCGCGGGAACGCACCACGCGGCTGGGTCCAAGAATGC
  CTCAGGATGGATCAGATGACGAGGACAAGGAGTGGCCCACCCTGGAGAAGGCTGCCACAA
  TGACAGCAGCGGGCCATCATGCAGAGGTGGTTGTGGACCCTGAGGATGAGCGTGCCATAG
  AGATGTTCATGAACAAGAACCCTCCTGCCAGGCGCACCCTGGCTGACATCATCATGGAGA
  AGCTGACTGAGAAGCAGACAGAGGTTGAGACAGTCATGTCAGAGGTGTCGGGCTTCCCTA
  TGCCCCAGCTGGACCCCCGGGTCCTAGAAGTGTACAGGGGGGTCCGGGAGGTATTATCTA
  AGTACCGCAGTGGAAAACTGCCCAAGGCATTTAAGATCATCCCTGCACTCTNNCACTGGG
  AGCANATCCTCTACGTCACAGAGCCGGGAGCCTGGACTGCAGCTGNCATGTACCANGCCA
  CCCAGNATTTTTGCCTCTAACCCTGAGGAACGCATGGNCCAGCGCTTCTACAACCTTGTN
  CTGCTCCCTCGAGTACGAGATGACGTTGCTGAATACCAACGACTCAACTTNCATCTCTAC
  ATGGCTCTCAAGAAGGCCCTTTTCAACCTGGAGCCCTGGTCANAGGGATCCTGATNCACT
  GTGCGAGTCTGGCACTTGTACCTNCGGNNAGCATCATGTGGNTAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004053 unedited
  CAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTCAATAAATATCTGTCTTTGCCTTTATC
  CACAGTGACCTGACACAATAGCCATAGAAAAGTACTCACACCAAGCCCCAGCTGGGGACT
  GGGAATGCTAGCATATGGTATCTCAAGTTGGCTCTCAGAACTAAACAGGGATAAGGGCCT
  AGAATGGAAGAGGGAACCAGCCAGACCCTCAGTCCTTCCTGTCCTGGACTGGGAGCCACA
  GATGTCCCTGTGATCTGTCACTGCCCTGATCTGGGTCTTCAGCCATTAAAGCTCAGTGTC
  ATCTTCAGTCACCAACGGGGGTCTTGGTGTCCTTCCAAACCCCTTTGGCCAGGACAGCTG
  ACTGTTTTCCTCACTCCACGGTGATGGGAACATCTTCCACATCGCGGGAGACTGCACTCT
  GAAGCTCACGCCTGATTTCGGGCGATAGCTGTGGATGGGGCTGCAGCCGGAGCAGTTCTA
  AGAGGGCCTCTTTCTGGTCTGTGGCCAAGTCGGCCTTGTAGCGCTGGACCAAAGTCAGGA
  GGCACTGGTGCCACAGCACAGGCAGTTCACGCTTCTCTGTCCGGAACCCCAGGAAGTGGA
  AGACTAGGGCATCCAGCACCCGGTAAGGCAGTGCATACTTCTTTATCCAGCAGCAGTCGC
  AGGAAGATGCTTGTTGGCACCGCTGTATTTCCATCTCAGCAATTTTCAGCATGGCCGCCC
  TGAAGTGCACCACCAGGAATGGAGCCCTCGGTGATGATGCTACCCCCATGATGGCTTCCC
  GGAGGGTACAGGGCCAGACTGCACAGTGGAATAAGATCCCTTTGACCCAGCTTCAGGTTG
  AAAAGGCCTTCTGAAACCCTGTAAAAATGAATTGGATCTTTGTATCACAACGTATCTCTA
  CTTAAGGACAGAAAGGTTGAAACCGTCTGGCATGCTCTTTAGTAGAGCAAAACCGGGGCT
  CGACATGAATGAATCAACCTCGTTTGGACAAAGATCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004053
• Insert Size: 1790 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004053.3, NP_004044.3
• RefSeq Size: 2005 bp
• RefSeq ORF: 1314 bp
• Locus ID: 705
• Cytogenetics: 6p21.1
• Protein Families: Stem cell - Pluripotency
• Gene Summary: 'Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi. [provided by RefSeq, Jul 2008]'