p27 KIP 1 (CDKN1B) (NM_004064) Human Untagged Clone

CAT#: SC117607

CDKN1B (untagged)-Human cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDKN1B
• Synonyms: CDKN4; KIP1; MEN1B; MEN4; P27KIP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004064 edited
  GAATTCGCCATATTGGGCCACTAAAAAAAGGGGGCTCGTCTTTTCGGGGTGTTTTTCTCCCCCTCCCCTG
  TCCCCGCTTGCTCACGGCTCTGCGACTCCGACGCCGGCAAGGTTTGGAGAGCGGCTGGGTTCGCGGGACC
  CGCGGGCTTGCACCCGCCCAGACTCGGACGGGCTTTGCCACCCTCTCCGCTTGCCTGGTCCCCTCTCCTC
  TCCGCCCTCCCGCTCGCCAGTCCATTTGATCAGCGGAGACTCGGCGGCCGGGCCGGGGCTTCCCCGCAGC
  CCCTGCGCGCTCCTAGAGCTCGGGCCGTGGCTCGTCGGGGTCTGTGTCTTTTGGCTCCGAGGGCAGTCGC
  TGGGCTTCCGAGAGGGGTTCGGGCTGCGTAGGGGCGCTTTGTTTTGTTCGGTTTTGTTTTTTTGAGAGTG
  CGAGAGAGGCGGTCGTGCAGACCCGGGAGAAAGATGTCAAACGTGCGAGTGTCTAACGGGAGCCCTAGCC
  TGGAGCGGATGGACGCCAGGCAGGCGGAGCACCCCAAGCCCTCGGCCTGCAGGAACCTCTTCGGCCCGGT
  GGACCACGAAGAGTTAACCCGGGACTTGGAGAAGCACTGCAGAGACATGGAAGAGGCGAGCCAGCGCAAG
  TGGAATTTCGATTTTCAGAATCACAAACCCCTAGAGGGCAAGTACGAGTGGCAAGAGGTGGAGAAGGGCA
  GCTTGCCCGAGTTCTACTACAGACCCCCGCGGCCCCCCAAAGGTGCCTGCAAGGTGCCGGCGCAGGAGAG
  CCAGGATGTCAGCGGGAGCCGCCCGGCGGCGCCTTTAATTGGGGCTCCGGCTAACTCTGAGGACACGCAT
  TTGGTGGACCCAAAGACTGATCCGTCGGACAGCCAGACGGGGTTAGCGGAGCAATGCGCAGGAATAAGGA
  AGCGACCTGCAACCGACGATTCTTCTACTCAAAACAAAAGAGCCAACAGAACAGAAGAAAATGTTTCAGA
  CGGTTCCCCAAATGCCGGTTCTGTGGAGCAGACGCCCAAGAAGCCTGGCCTCAGAAGACGTCAAACGTAA
  ACAGCTCGAATTAAGAATATGTTTCCTTGTTTATCAGATACATCACTGCTTGATGAAGCAAGGAAGATAT
  ACATGAAAATTTTAAAAATACATATCGCTGACTTCATGGAATGGACATCCTGTATAAGCACTGAAAAACA
  ACAACACAATAACACTAAAATTTTAGGCACTCTTAAATGATCTGCCTCTAAAAGCGTTGGATGTAGCATT
  ATGCAATTAGGTTTTTCCTTATTTGCTTCATTGTACTACCTGTGTATATAGTTTTTACCTTTTATGTAGC
  ACATAAACTTTGGGGAAGGGAGGGCAGGGTGGGGCTGAGGAACTGACGTGGAGCGGGGTATGAAGAGCTT
  GCTTTGATTTACAGCAAGTAGATAAATATTTGACTTGCATGAAGAGAAGCAATTTTGGGGAAGGGTTTGA
  ATTGTTTTCTTTAAAGATGTAATGTCCCTTTCAGAGACAGCTGATACTTCATTTAAAAAAATCACAAAAA
  TTTGAACACTGGCTAAAGATAATTGCTATTTATTTTTACAAGAAGTTTATTCTCATTTGGGAGATCTGGT
  GATCTCCCAAGCTATCTAAAGTTTGTTAGATAGCTGCATGTGGCTTTTTTAAAAAAGCAACAGAAACCTA
  TCCTCACTGCCCTCCCCAGTCTCTCTTAAAGTTGGAATTTACCAGTTAATTACTCAGCAGAATGGTGATC
  ACTCCAGGTAGTTTGGGGCAAAAATCCGAGGTGCTTGGGAGTTTTGAATGTTAAGAATTGACCATCTGCT
  TTTATTAAATTTGTTGACAAAATTTTCTCATTTTCTTTTCACTTCGGGCTGTGTAAACACAGTCAAAATA
  ATTCTAAATCCCTCGATATTTTTAAAGATCTGTAAGTAACTTCACATTAAAAAATGAAATATTTTTTAAT
  TTAAAGCTTACTCTGTCCATTTATCCACAGGAAAGTGTTATTTTTAAAGGAAGGTTCATGTAGAGAAAAG
  CACACTTGTAGGATAAGTGAAATGGATACTACATCTTTAAACAGTATTTCATTGCCTGTGTATGGAAAAA
  CCATTTGAAGTGTACCTGTGTACATAACTCTGTAAAAACACTGAAAAATTATACTAACTTATTTATGTTA
  AAAGATTTTTTTTAATCTAGACAATATACAAGCCAAAGTGGCATGTTTTGTGCATTTGTAAATGCTGTGT
  TGGGTAGAATAGGTTTTCCCCTCTTTTGTTAAATAATATGGCTATGCTTAAAAGGTTGCATACTGAGCCA
  AGTATAATTTTTTGTAATGTGTGAAAAAGATGCCAATTATTGTTACACATTAAGTAATCAATAAAGAAAA
  CTTCCATAGCTAAAAAAAAAAAAAAAAAAACTCGAC
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004064 unedited
  TCTTTCCCCGCCCGTTGCCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG
  CAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCG
  AATTCGGCACGAGGGGCCACTAAAAAAAGGGGGCTCGTCTTTTCGGGGTGTTTTTCTCCC
  CCTCCCCTGTCCCCGCTTGCTCACGGCTCTGCGACTCCGACGCCGGCAAGGTTTGGAGAG
  CGGCTGGGTTCGCGGGACCCGCGGGCTTGCACCCGCCCAGACTCGGACGGGCTTTGCCAC
  CCTCTCCGCTTGCCTGGTCCCCTCTCCTCTCCGCCCTCCCGCTCGCCAGTCCATTTGATC
  AGCGGAGACTCGGCGGCCGGGCCGGGGCTTCCCCGCAGCCCCTGCGCGCTCCTAGAGCTC
  GGGCCGTGGCTCGTCGGGGTCTGTGTCTTTTGGCTCCGAGGGCAGTCGCTGGGCTTCCGA
  GAGGGGTTCGGGCTGCGTAGGGGCGCTTTGTTTTGTTCGGTTTTGTTTTTTTGAGAGTGC
  GAGAGAGGCGGTCGTGCAGACCCGGGAGAAAGATGTCAAACGTGCGAGTGTCTAACGGGA
  GCCCTAGCCTGGAGCGGATGGACGCCAGGCAGGCGGAGCACCCCAAGCCCTCGGCCTGCA
  GGAACCTCTTCGGCCCGGTGGACCACGAAGAGTTAACCCGGGACTTGGAGAAGCACTGCA
  GAGACATGGAAGAGGCGAGCCAGCGCAAGTGGAATTTCGATTTTCAGAATCACAAACCCC
  TAGAGGGCAAGTACGAGTGGCAAGAGGTGGAGAAGGGCANCTTGCCCGATTCTACTACAG
  ACCCCCGCGGCCCCCCAAAGTGCCTGCCAGGTGCCCCGCGCAGANAGCANGATGTCAGCG
  GGAGCCGCCCCGNCGGCGCCTTTATTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004064 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAGCTATGGAAGTTTTCTTTAT
  TGATTACTTAATGTGTAACAATAATTGGCATCTTTTTCACACATTACAAAAAATTATACT
  TGGCTCAGTATGCAACCTTTTAAGCATAGCCATATTATTTAACAAAAGAGGGGAAAACCT
  ATTCTACCCAACACAGCATTTACAAATGCACAAAACATGCCACTTTGGCTTGTATATTGT
  CTAGATTAAAAAAAATCTTTTAACATAAATAAGTTAGTATAATTTTTCAGTGTTTTTACA
  GAGTTATGTACACAGGTACACTTCAAATGGTTTTTCCATACACAGGCAATGAAATACTGT
  TTAAAGATGTAGTATCCATTTCACTTATCCTACAAGTGTGCTTTTCTCTACATGAACCTT
  CCTTTAAAAATAACACTTTCCTGTGGATAAATGGACAGAGTAAGCTTTAAATTAAAAAAT
  ATTTCATTNTTTAATGTGAAGTTACTTACAGATCTTTAAAAATATCGAGGGATTTAGAAT
  TATTTTGACTGTGTTTACACAGCCCGAAGTGAAAAGAAAATGAGAAAATTTTGTCAACAA
  ATTTAATAAAAGCAGATGGTCAATTCTTAACATTCAAACTCCCAAGCACCTCGGATTTTT
  GCCCCAACTACCTGGAGTGATCACCATTCTGCTGAGTAANTAACTGGTAAAATCCAACTT
  TAGAGAGACTGGNGAGGGCAGTGAGGATAGTTTTCTGNTGCTTTTTTAAAAAGCCACATG
  CAGCTATCTACCAAACTTTAGATAGCTTGGGAGATCACCAAATCTTCCAAATGAGAATAA
  AACTCCTGGAAAAATAAATAGCAATTATCTTTAGCCACGGGTCCAAATTTTGGGGATTTT
  TTTAAATGAAAGATCAGCTGTCTCTGGAAGGGGACTTACATTCTTTAAGAAACCATTCAA
  ACCCTTTCCCAAAATGCTTCCCTTCTGCAAGACAAATTTATTTATTGCTGGAAATAAAGC
  AGCCTTATACCCGCTCCGAGGAGTTCTTCAGCCCCCCTGCCTCCTTCCAAAGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004064
• ORF Size: 597 bp
• Insert Size: 2500
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_004064.2, NP_004055.1
• RefSeq Size: 2422
• RefSeq ORF: 597
• Locus ID: 1027
• Domains: CDI
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, ErbB signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer
• Gene Summary: This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]