ST13 (NM_003932) Human Untagged Clone

CAT#: SC117700

ST13 (untagged)-Human suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein) (ST13)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ST13
• Synonyms: AAG2; FAM10A1; FAM10A4; HIP; HOP; HSPABP; HSPABP1; P48; PRO0786; SNC6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117700 sequence for NM_003932 edited (data generated by NextGen Sequencing)
  ATGGACCCCCGCAAAGTGAACGAGCTTCGGGCCTTTGTGAAAATGTGTAAGCAGGATCCG
  AGCGTTCTGCACACCGAGGAAATGCGCTTCCTGAGGGAGTGGGTGGAGAGCATGGGTGGT
  AAAGTACCACCTGCTACTCAGAAAGCTAAATCAGAAGAAAATACCAAGGAAGAAAAACCT
  GATAGTAAGAAGGTGGAGGAAGACTTAAAGGCAGACGAACCATCAAGTGAGGAAAGTGAT
  CTAGAAATTGATAAAGAAGGTGTGATTGAACCAGACACTGATGCTCCTCAAGAAATGGGA
  GATGAAAATGCGGAGATAACGGAGGAGATGATGGATCAGGCAAATGATAAAAAAGTGGCT
  GCTATTGAAGCCCTAAATGATGGTGAACTCCAGAAAGCCATTGACTTATTCACAGATGCC
  ATCAAGCTGAATCCTCGCTTGGCCATTTTGTATGCCAAGAGGGCCAGTGTCTTCGTCAAA
  TTACAGAAGCCAAATGCTGCCATCCGAGACTGTGACAGAGCCATTGAAATAAATCCTGAT
  TCAGCTCAGCCTTACAAGTGGCGGGGGAAAGCACACAGACTTCTAGGCCACTGGGAAGAA
  GCAGCCCATGATCTTGCCCTTGCCTGTAAATTGGATTATGATGAAGATGCTAGTGCAATG
  CTGAAAGAAGTTCAACCTAGGGCACAGAAAATTGCAGAACATCGGAGAAAGTATGAGCGA
  AAACGTGAAGAGCGAGAGATCAAAGAAAGAATAGAACGAGTTAAGAAGGCTCGAGAAGAG
  CATGAGAGAGCCCAGAGGGAGGAAGAAGCCAGACGACAGTCAGGAGCTCAGTATGGCTCT
  TTTCCAGGTGGCTTTCCTGGGGGAATGCCTGGTAATTTTCCCGGAGGAATGCCTGGAATG
  GGAGGGGGCATGCCTGGAATGGCTGGAATGCCTGGACTCAATGAAATTCTTAGTGATCCA
  GAGGTTCTTGCAGCCATGCAGGATCCAGAAGTTATGGTGGCTTTCCAGGATGTGGCTCAG
  AACCCAGCAAATATGTCAAAATACCAGAGCAACCCAAAGGTTATGAATCTCATCAGTAAA
  TTGTCAGCCAAATTTGGAGGTCAAGCGTAA
  
  Clone variation with respect to NM_003932.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003932 unedited
  GGGGCGGCCCCCTTTCTCCCCCCCCCCGTTCAAATTTGTATACGACTCACTATAGGCGGC
  CGCGNAATCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGCCGCCCCCTTCTGCGCGG
  TCACGCCGAGCCAGCGCCTGGGCCTGGAACCGGGCCGTAGCCCCCCCAGTTTCGCCCACC
  ACCTCCCTACCATGGACCCCCGCAAAGTGAACGAGCTTCGGGCCTTTGTGAAAATGTGTA
  AGCAGGATCCGAGCGTTCTGCACACCGAGGAAATGCGCTTCCTGAGGGAGTGGGTGGAGA
  GCATGGGTGGTAAAGTACCACCTGCTACTCAGAAAGCTAAATCAGAAGAAAATACCAAGG
  AAGAAAAACCTGATAGTAAGAAGGTGGAGGAAGACTTAAAGGCAGACGAACCATCAAGTG
  AGGAAAGTGATCTAGAAATTGATAAAGAAGGTGTGATTGAACCAGACACTGATGCTCCTC
  AAGAAATGGGAGATGAAAATGCGGAGATAACGGAGGAGATGATGGATCAGGCAAATGATA
  AAAAAGTGGCTGCTATTGAAGCCCTAAATGATGGTGAACTCCAGAAAGCCATTGACTTAT
  TCACAGATGCCATCAAGCTGAATCCTCGCTTGGCCATTTTGTATGCCAAGAGGGCCAGTG
  TCTTCGTCAATTACAGAAGCCAAATGCTGCCATCCGAGACTGTGACAGAGCCATTGAATA
  AATCCTGATTCAGCTCAGCCTTACCAGTGGCGGGGGAAAGCACACAGACTCCTAGGCCAC
  TGGGAAGAAGCAGCCCATGATCTTGCCTTTGCTGTAAATGGGATATGATGAAAATGCTAN
  TGCATTGCTAAAGGAGTTCACCTAGGGCCCAAAAAATTGCNAACATCNCGAAAAGTTTGA
  GCGAAAACCGGGAGAGCGGGAGATCCAAGAAAGAATTNAACGGTTTAAAAGGCTTCGCAA
  AAACCTGAAAAACCCCCAGGGAGGAAAAANCCNACAACGGCCGGAGCTCAANTTGGTTCT
  TTTCCAGGGGCTTTCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003932 unedited
  GGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAGNAAAAGGAATT
  TTACTTTTACCCACTTATGGTAATATTTGCAATATTAGAATATAATTAGGTTAAATAAAT
  CAAATAAGGAATCCAGAAGAAACTGTTCTTGAGTTAAGCCAAGTACACACTGGCAATTTC
  GAGAAAGGAGAACTCTACACATTAACAAAAGAACACACAAGCTCAAATGCAAGTTTATAT
  GGTGAGTGTTTTTAAAGGATTTAAAAAGCATGCTCCATCAGCATAATAGCTTTTTCTCAT
  TTATGTAACAGATGCATTTCTGTCTGCTTGTGTACAGCAAAGGGTTAGAACCTGAATAAA
  CTTATTTATGATTTCAGAAATGTGGTATTTTAGAAATGTACTCCATAGAATTTACTTCAC
  TGCTCCTACCACTGATTCTTCAAATAAGCAAAGTACAGAAGGCCTTATCCCTTGAAAATG
  TTTTTAAACACTAGCACTTTAAATTGAAACACTGGTAGAAACGTAGCAATGTCAGGTACA
  ATGCCTATACTTAGTCTTATTTAAAATAAGAATTGAATGCACAACAGAAAATTTATCATT
  TTCTTTATCATTGGAGGGGAGGGAAAGAAAGCAAGCAGAGGTGGGGATATAGTTACACTA
  TTTAAGAGCTGAATACAAAGACAATGAGTCTTTCATAGTCTCAACAACTCCACAATGAAT
  AACTGTGGATTATCTCATTTATAATTCACATTGGATAAAAAAATCGGTTCCCCTCTGTGT
  GTATCAATTTAATTCAAACCTTATCTTCAGAATACCAAGATGAATACTACAAGATNAACT
  AACTGGCATAGGGTTTTTCTAAGTAGAAGACCAAACACCAAGGGAATCCCTCTTTNNCAT
  GTCTCTGATAGGGGCAGATTTTGCAGATGGAAGTCTACACTTAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003932
• Insert Size: 3080 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003932.3, NP_003923.2
• RefSeq Size: 3214 bp
• RefSeq ORF: 1110 bp
• Locus ID: 6767
• Cytogenetics: 22q13.2
• Domains: TPR, STI1
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is an adaptor protein that mediates the association of the heat shock proteins HSP70 and HSP90. This protein has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones. The expression of this gene is reported to be downregulated in colorectal carcinoma tissue suggesting that it is a candidate tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]'
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.