SPINT1 (NM_003710) Human Untagged Clone

CAT#: SC117783

SPINT1 (untagged)-Human serine peptidase inhibitor, Kunitz type 1 (SPINT1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPINT1
• Synonyms: HAI; HAI1; MANSC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003710, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCCCTGCGAGGACGATGGCCCGCGCCCGCCTCGCCCCGGCCGGCATCCCTGCCGTCGCCTTGTGGC
  TTCTGTGCACGCTCGGCCTCCAGGGCACCCAGGCCGGGCCACCGCCCGCGCCCCCTGGGCTGCCCGCGGG
  AGCCGACTGCCTGAACAGCTTTACCGCCGGGGTGCCTGGCTTCGTGCTGGACACCAACGCCTCGGTCAGC
  AACGGAGCTACCTTCCTGGAGTCCCCCACCGTGCGCCGGGGCTGGGACTGCGTGCGCGCCTGCTGCACCA
  CCCAGAACTGCAACTTGGCGCTAGTGGAGCTGCAGCCCGACCGCGGGGAGGACGCCATCGCCGCCTGCTT
  CCTCATCAACTGCCTCTACGAGCAGAACTTCGTGTGCAAGTTCGCGCCCAGGGAGGGCTTCATCAACTAC
  CTCACGAGGGAAGTGTACCGCTCCTACCGCCAGCTGCGGACCCAGGGCTTTGGAGGGTCTGGGATCCCCA
  AGGCCTGGGCAGGCATAGACTTGAAGGTACAACCCCAGGAACCCCTGGTGCTGAAGGATGTGGAAAACAC
  AGATTGGCGCCTACTGCGGGGTGACACGGATGTCAGGGTAGAGAGGAAAGACCCAAACCAGGTGGAACTG
  TGGGGACTCAAGGAAGGCACCTACCTGTTCCAGCTGACAGTGACTAGCTCAGACCACCCAGAGGACACGG
  CCAACGTCACAGTCACTGTGCTGTCCACCAAGCAGACAGAAGACTACTGCCTCGCATCCAACAAGGTGGG
  TCGCTGCCGGGGCTCTTTCCCACGCTGGTACTATGACCCCACGGAGCAGATCTGCAAGAGTTTCGTTTAT
  GGAGGCTGCTTGGGCAACAAGAACAACTACCTTCGGGAAGAAGAGTGCATTCTAGCCTGTCGGGGTGTGC
  AAGGCCCCTCCATGGAAAGGCGCCATCCAGTGTGCTCTGGCACCTGTCAGCCCACCCAGTTCCGCTGCAG
  CAATGGCTGCTGCATCGACAGTTTCCTGGAGTGTGACGACACCCCCAACTGCCCCGACGCCTCCGACGAG
  GCTGCCTGTGAAAAATACACGAGTGGCTTTGACGAGCTCCAGCGCATCCATTTCCCCAGTGACAAAGGGC
  ACTGCGTGGACCTGCCAGACACAGGACTCTGCAAGGAGAGCATCCCGCGCTGGTACTACAACCCCTTCAG
  CGAACACTGCGCCCGCTTTACCTATGGTGGTTGTTACGGCAACAAGAACAACTTTGAGGAAGAGCAGCAG
  TGCCTCGAGTCTTGTCGCGGCATCTCCAAGAAGGATGTGTTTGGCCTGAGGCGGGAAATCCCCATTCCCA
  GCACAGGCTCTGTGGAGATGGCTGTCGCAGTGTTCCTGGTCATCTGCATTGTGGTGGTGGTAGCCATCTT
  GGGTTACTGCTTCTTCAAGAACCAGAGAAAGGACTTCCACGGACACCACCACCACCCACCACCCACCCCT
  GCCAGCTCCACTGTCTCCACTACCGAGGACACGGAGCACCTGGTCTATAACCACACCACGCGGCCCCTCT
  GA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003710 unedited
  GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCACCCTC
  GGAACCCAGAGGCCCGCGCTCTGAAGGTGACCCCCCTGGGGAGGAAGGCGATGGCCCCTG
  CGAGGACGATGGCCCGCGCCCGCCTCGCCCCGGCCGGCATCCCTGCCGTCGCCTTGTGGC
  TTCTGTGCACGCTCGGCCTCCAGGGCACCCAGGCCGGGCCACCGCCCGCGCCCCCTGGGC
  TGCCCGCGGGAGCCGACTGCCTGAACAGCTTTACCGCCGGGGTGCCTGGCTTCGTGCTGG
  ACACCAACGCCTCGGTCAGCAACGGAGCTACCTTCCTGGAGTCCCCCACCGTGCGCCGGG
  GCTGGGACTGCGTGCGCGCCTGCTGCACCACCCAGAACTGCAACTTGGCGCTAGTGGAGC
  TGCAGCCCGACCGCGGGGAGGACGCCATCGCCGCCTGCTTCCTCATCAACTGCCTCTACG
  AGCAGAACTTCGTGTGCAAGTTCGCGCCCAGGGAGGGCTTCATCAACTACCTCACGAGGG
  AAGTGTACCGCTCCTACCGCCAGCTGCGGACCCAGGGCTTTGGAGGGTCTGGGATCCCCA
  AGGCCTGNGCAGGCATAGACTTGAAGGTACAACCCCAGGAACCCCTGGTGCTGAAGGATG
  TGGAAAACACAGATTGGCGCCTACTGCGGNGTGACACGGATGTCAGGGTAGAGAGGAAAG
  ACCCAAACCCAGTGGAACTGTGGGGACTCAAGGAAGCACCTACCTGTCCCAGCTGACAGT
  GACTAGCTCACACCACCCAGAGACACGGNCAACGTCACAGTCACTGGGCTGTCCACCAAC
  CAAAAAGAAAACACTGCCTCGCATCCAACAAGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003710 unedited
  ATACTGTGGCTGGGACAGTCCATGTCTGTANAACCTTGGGGGTGGGGGCTGCGGAATGCA
  GCTGAGCCTCTCCTGGCTCTGTCTGCTGGTCTAGGCCAGGGTGGGGCTGATCAAGGGCAG
  AGAGCTCAATCTTGGGGGAAGAGGAAGAGAGGACAGAGAGGCCAAACAGGCCTCTTCCCC
  TCCTCTTCACCCATGCCACAGCATTAAATAAACAAAAAGCAACTCTTTACAGCACAAACT
  ACACAGGGAAGTCCTTCCTCCCAGCCCTGGGCGCACAGCATGGAAGAGGAGCTCGGGAGG
  ACAGGGTTGGGGCCTCCAGGGTCTGACACCACCCCACTCCTCTTCCTAGCACCATGGGGT
  AGGGACAGGCAGCTTTGAACAGAGGAAACAAAACTCCAACTTCCTCCAAACTCCTATGCA
  GGCAGGTCCATCTAGTCTGGTACTTCTGGCCCAAGGGTTTTCTGCTCCTTCCAAACCTTT
  GAGCTTTCTCAGGACGTGAGCTTAGCTGAGACTTCTCCAAGAGGGGCTGGAGCCCTGGTC
  TCTGAGGCACAGTGGGCCTGGGAAACAGGCAAGAGTCTGGTTCCAAAGTTTTTCCCAGCC
  CAGGCCTCTGCCTTGGCAAGCAGGAAGCAGGGCCAGGTGAGAGCCGGTGAGACCCAGGCT
  CANAGGGGCCGGGTGGTGTGGTTATAGACCAGGTGCTCCGTGTCCTCGGTAGTGGAGACA
  GTGGANNCTGCAGGNGTGGGTGGTGGTGGGNGNTNNGTNNNCCNNNGNNAATNCCCTTTT
  CTGGGTTCTGAAAAAAGCATTACCCCAAGATGGGCTACCACCACCCCCAATGCAGATGGA
  CCAGGAACCACTGGGACAGGCCATCCTCCCACAGAGCCCGGGGCTGGGGATTGGGGGATT
  TTCCCGGGCTCAGGCCCAACCACATCCCTTCTTTGGAGATGCCCGGGACAAGAACTCTGA
  GGCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003710
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003710.3, NP_003701.1
• RefSeq Size: 2436 bp
• RefSeq ORF: 1542 bp
• Locus ID: 6692
• Cytogenetics: 15q15.1
• Domains: ldl_recept_a, KU
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2).