RAD54 (RAD54L) (NM_003579) Human Untagged Clone

CAT#: SC117863

RAD54L (untagged)-Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAD54L
• Synonyms: hHR54; HR54; hRAD54; RAD54A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117863 sequence for NM_003579 edited (data generated by NextGen Sequencing)
  ATGAGGAGGAGCTTGGCTCCCAGCCAGCTGGCCAAGAGAAAACCTGAAGGCAGGTCCTGT
  GATGATGAAGACTGGCAACCTGGCCTAGTGACTCCTAGGAAACGGAAATCCAGCAGTGAG
  ACCCAGATCCAGGAGTGTTTCCTGTCTCCTTTTCGGAAACCTTTGAGTCAGCTAACCAAT
  CAACCACCTTGTCTGGACAGCAGTCAGCATGAAGCATTTATTCGAAGCATTTTGTCAAAG
  CCTTTCAAAGTCCCCATTCCAAATTATCAAGGTCCTCTGGGCTCTCGAGCATTGGGCCTG
  AAAAGGGCTGGGGTCCGCCGGGCCCTCCATGACCCCCTGGAAAAAGATGCCTTGGTTCTG
  TATGAGCCTCCCCCGCTGAGCGCTCATGACCAGCTGAAGCTTGACAAGGAGAAACTCCCT
  GTCCATGTGGTTGTTGACCCTATTCTCAGTAAGGTTTTGCGGCCTCATCAGAGAGAGGGA
  GTGAAATTCCTGTGGGAGTGTGTCACCAGTCGGCGCATCCCTGGCAGCCATGGCTGCATC
  ATGGCTGATGAGATGGGCCTAGGAAAGACGCTGCAGTGCATCACATTGATGTGGACACTT
  TTACGCCAGAGTCCAGAGTGCAAGCCAGAAATTGACAAGGCAGTGGTGGTGTCGCCTTCC
  AGCCTGGTGAAGAACTGGTACAATGAGGTTGGGAAATGGCTCGGAGGGAGGATCCAACCT
  CTGGCCATCGATGGAGGATCTAAGGATGAAATAGACCAAAAGCTGGAAGGATTCATGAAC
  CAGCGTGGAGCCAGGGTGTCTTCTCCCATCCTCATCATTTCCTATGAGACCTTCCGCCTT
  CATGTTGGAGTCCTCCAGAAAGGAAGTGTTGGTCTGGTCATATGTGACGAGGGACACAGG
  CTCAAGAACTCTGAGAATCAGACTTACCAAGCCCTGGACAGCTTGAACACCAGCCGGCGG
  GTGCTCATCTCCGGAACTCCCATCCAGAATGATCTGCTTGAGTATTTCAGCTTGGTACAT
  TTTGTTAATTCCGGCATCCTAGGGACTGCCCATGAATTCAAGAAGCATTTTGAATTGCCA
  ATTTTGAAGGGTCGAGACGCTGCTGCTAGTGAGGCAGACAGGCAGCTAGGAGAGGAGCGG
  CTGCGGGAGCTCACCAGCATTGTGAATAGATGCCTGATACGGAGGACTTCTGATATCCTT
  TCTAAATATCTGCCTGTGAAGATTGAGCAGGTCGTTTGTTGTAGGCTGACACCCCTTCAG
  ACTGAGTTATACAAGAGGTTTCTGAGACAAGCCAAACCGGCAGAAGAATTGCTTGAGGGC
  AAGATGAGTGTGTCTTCCCTTTCTTCCATCACCTCGCTAAAGAAGCTTTGTAATCATCCA
  GCTCTAATCTATGATAAGTGTGTGGAAGAGGAGGATGGCTTTGTGGGTGCCTTGGACCTC
  TTCCCTCCTGGTTACAGCTCTAAGGCCCTGGAGCCCCAGCTGTCAGGTAAGATGCTGGTC
  CTGGATTATATTCTGGCGGTGACCCGAAGCCGTAGCAGTGACAAAGTAGTGCTGGTGTCG
  AATTACACCCAGACTTTGGATCTCTTTGAGAAGCTGTGCCGTGCCCGAAGGTACTTATAC
  GTCCGCCTGGATGGCACGATGTCCATTAAGAAGCGAGCCAAGGTTGTAGAACGCTTCAAT
  AGTCCATCGAGCCCTGACTTTGTCTTCATGCTGAGCAGCAAAGCTGGGGGCTGTGGCCTC
  AATCTCATTGGGGCTAACCGGCTGGTCATGTTTGACCCTGACTGGAACCCAGCCAATGAT
  GAACAAGCCATGGCCCGGGTCTGGCGAGATGGTCAAAAGAAGACTTGCTATATCTACCGC
  CTGCTGTCTGCAGGGACCATTGAGGAGAAGATCTTCCAGCGTCAGAGCCACAAGAAGGCA
  CTGAGCAGCTGTGTGGTGGATGAGGAGCAGGATGTAGAGCGCCACTTCTCTCTGGGCGAG
  TTGAAGGAGCTGTTTATCCTGGATGAAGCTAGCCTCAGTGACACACATGACAGGTTGCAC
  TGCCGACGTTGTGTCAACAGCCGTCAGATCCGGCCACCCCCTGATGGTTCTGACTGCACT
  TCAGACCTGGCAGGGTGGAACCACTGCACTGATAAGTGGGGGCTCCGGGATGAGGTACTC
  CAGGCTGCCTGGGATGCTGCCTCCACTGCCATCACCTTCGTCTTCCACCAGCGTTCTCAT
  GAGGAGCAGCGGGGCCTCCGCTGA
  
  Clone variation with respect to NM_003579.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003579 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCTCGTCTCGGCT
  TATTGGGGACGGCCACTCTCACAGTTTGGTTCCAAACACCAGTTCCTGGATGGATTCCCG
  CCATCCATGCCCCCTCTTTAATTAGCCGGTCCTCTCAATAATGTAGCAGCCCCCTCTACA
  GATTAGACCCTGGTCCTACACTCTTAGCCGCTGCCTGCTTTTGACCTTTGGCTCATGGGT
  ACTTGACGTTTTAAACTCCTAGGCCCAGGATGAGGAGGAGCTTGGCTCCCAGCCAGCTGG
  CCAAGAGAAAACCTGAAGGCAGGTCCTGTGATGATGAAGACTGGCAACCTGGCCTAGTGA
  CTCCTAGGAAACGGAAATCCAGCAGTGAGACCCAGATCCAGGAGTGTTTCCTGTCTCCTT
  TTCGGAAACCTTTGAGTCAGCTAACCAATCAACCACCTTGTCTGGACAGCAGTCAGCATG
  AAGCATTTATTCGAAGCATTTTGTCAAAGCCTTTCAAAGTCCCCATTCCAAATTATCAAG
  GTCCTCTGGGCTCTCGAGCATTGGGCCTGAAAAGGGCTGGGGTCCGCCGGGCCCTCCATG
  ACCCCCTGGAAAAAGATGCCTTGGTTCTGTATGAGCCTCCCCCGCTGAGCGCTCATGACC
  AGCTGAAGCTTGACAAGGAGAAACTCCCTGTCCATGTGGTTGTTGACCCTATTCTCAGTA
  AGGGTTTGCGGCCTCATCAGAGAGAGGGAGTGAAATTCCTGTGGGAGTGTGTCACCAGTC
  GGCGCATCCCTGGCAGCCATGGCTGCATCATGGCTGATGAGATGGGCCTANGAAAGACGC
  TGCAGTGCATCACATTGATGTGGACACNTTTACGCCAGAGTCCAGAGTGCAAGCANAAAT
  GACAGGCAGTGGTGGTGTCGCCTTCCACCTGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003579 unedited
  GGGGGGTTTTGTNNTTTTTTTTTTTTATTTTTCTTTTTTTTTTCCTAACCTTTTTCTTTT
  TTTTAACCAAAAAGTTTTTCCTTATAAAATAAATTTGGGCAAACACCATGCAGCCCTTCT
  TGATGATCTTCTCCCACCACAACAAAATTCAACAGGCGCCCTGTGCGAGCAAGCGTCCCC
  CCTTCCCCCTACCTCCTCCCCTTTCAACACCTTCACCCCACACCCACCTCTTCCTCCCCC
  CGTATGCCTCCTTTGCTCCCTTTTCCCCCACCCCTCTCCCCTCACCCCCCAGGTTCCCTC
  GCACCTGACCGCCATCTCTCCCCTCGCTTTCCTTCTCCTTCCTTCCTCTCTCCCCTCCCT
  ATCCCCTCTTTTTTCTCCCCCCCCCCCCCCCCCCTGTTCTTTTTCCCTTCTTGCCTTACC
  TTCCCTCCTCCCCCTCCGTTCGTTTTTCTTCTCTTCCCCTCCTTCATTTCTCTCTCCCCT
  CCACTTTCCTCACCTCCCCCATCCTTCCTTCTCACCTCTTCCTACCCTTTACCTCTAAAC
  TATCTAACAACACACACTTTCTTCTCTCTCTCCCCACATATACCCAGATCACCCGTCCCC
  ACTACCCTTTCTTGTCGTTTCCTCTCCTCCCCCCCCCCCCCCCCCCCACACCCATCCCTC
  CTCCGCCCCTCTCTCCTCCTCTCTCTCTCCCCTCCTTCATTTCTCCTGCCACTCGCCCCA
  CCACTCCCACCTATCATCCCTCCTTATTNCACTACATACATTTTATAATTTCTTCCTCTC
  GCGCAACTTTCTCCCTACCTCCCCTTATCCTCCGATATCAATTCTTAATACCAACGCCTT
  ATGCAAACATTTATTTATATATTCCCACACTATCTCCCCGTCCATCCTCCTCTTTTTTCA
  CTCACTTCCATCTTTTTTTTCCCTCTCGTTGTCACCACCATTCTGTCCCCACCACTCTCC
  CCATTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003579
• ORF Size: 2244 bp
• Insert Size: 2820
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_003579.2, NP_003570.1
• RefSeq Size: 3107
• RefSeq ORF: 2244
• Locus ID: 8438
• Domains: SNF2_N, DEAD, helicase_C
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Homologous recombination
• Gene Summary: The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Dec 2008]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same isoform (1).