NSMAF (NM_003580) Human Untagged Clone

CAT#: SC117865

NSMAF (untagged)-Human neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NSMAF
• Synonyms: FAN; GRAMD5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117865 sequence for NM_003580 edited (data generated by NextGen Sequencing)
  ATGGCGTTTATCCGGAAGAAGCAGCAGGAGCAGCAGCTGCAGCTCTACTCCAAGGAGAGA
  TTTTCCTTGCTGCTGCTTAACTTGGAGGAGTACTACTTTGAACAGCATAGAGCCAATCAC
  ATTTTGCACAAGGGCAGTCACCATGAAAGGAAAATCAGAGGCTCCTTAAAAATATGTTCA
  AAATCGGTGATTTTTGAACCAGATTCAATATCCCAGCCCATCATCAAGATTCCTTTGAGA
  GACTGTATAAAAATAGGAAAGCATGGAGAAAATGGAGCCAATAGACACTTCACAAAGGCA
  AAATCTGGGGGTATTTCACTCATTTTCAGTCAGGTATATTTCATTAAAGAACATAATGTT
  GTTGCACCATATAAAATAGAAAGGGGCAAAATGGAATATGTTTTTGAATTGGATGTTCCC
  GGGAAAGTGGAAGATGTTGTGGAGACGTTGCTTCAGCTTCACAGAGCATCCTGCCTTGAC
  AAATTGGGTGACCAAACCGCCATGATAACAGCTATTTTGCAGTCTCGTTTAGCTAGAACA
  TCATTTGACAAAAACAGGTTCCAAAACATTTCTGAAAAGCTGCACATGGAATGCAAAGCA
  GAAATGGTGACGCCTCTGGTGACTAATCCTGGACACGTGTGCATCACGGACACAAACCTG
  TATTTTCAGCCCCTCAACGGCTACCCGAAACCTGTGGTCCAGATAACACTCCAAGATGTC
  CGCCGCATCTACAAAAGGAGGCACGGCCTCATGCCTCTGGGCTTGGAAGTATTTTGCACA
  GAAGATGATCTGTGTTCCGACATCTACCTAAAGTTCTATGAACCTCAAGATAGAGATGAT
  CTCTATTTTTACATTGCCACATACCTAGAGCACCATGTGGCGGAGCACACTGCTGAGAGC
  TACATGCTGCAGTGGCAGCGTGGACACCTTTCCAACTATCAGTACCTCCTTCACCTCAAC
  AACCTGGCCGACCGCAGCTGCAACGACCTCTCCCAGTACCCTGTGTTTCCATGGATAATA
  CATGATTATTCCAGCTCAGAACTAGATTTGTCAAATCCAGGAACCTTCCGGGATCTCAGT
  AAGCCAGTAGGGGCCCTAAATAAGGAACGGCTGGAGAGACTACTGACACGCTACCAGGAA
  ATGCCTGAACCAAAGTTCATGTATGGGAGTCACTACTCTTCCCCGGGTTATGTACTTTTT
  TATCTTGTTAGGATTGCACCAGAGTATATGCTGTGCCTGCAGAATGGAAGATTTGATAAT
  GCAGATAGAATGTTCAACAGTATTGCAGAAACTTGGAAAAACTGTCTGGATGGTGCAACG
  GATTTTAAAGAGTTAATTCCAGAATTCTATGGTGATGATGTGAGCTTTCTAGTCAATAGC
  CTGAAGTTGGATTTGGGAAAGAGACAAGGAGGACAGATGGTTGACGACGTGGAGCTTCCC
  CCTTGGGCTTCCAGTCCCGAGGACTTTCTCCAGAAGAGCAAAGATGCATTGGAAAGCAAT
  TATGTGTCTGAACACCTTCACGAGTGGATTGATCTAATATTTGGCTACAAACAAAAAGGG
  AGTGATGCAGTTGGGGCCCATAATGTATTTCATCCCCTGACCTATGAAGGAGGTGTAGAC
  TTGAACAGCATCCAGGATCCTGATGAGAAGGTAGCCATGCTTACGCAAATCTTGGAATTT
  GGGCAGACACCAAAACAACTATTTGTGACACCACATCCTCGAAGGATCACCCCAAAGTTT
  AAAAGTTTGTCCCAGACCTCCAGTTATAATGCTTCTATGGCAGATTCCCCAGGTGAAGAG
  TCTTTTGAAGACCTGACCGAAGAAAGCAAAACACTGGCCTGGAATAACATCACCAAACTG
  CAGTTACACGAGCACTATAAAATCCACAAAGAAGCAGTTACTGGAATCACGGTCTCTCGC
  AATGGATCTTCAGTATTCACAACATCCCAAGATTCCACCTTGAAGATGTTTTCTAAAGAA
  TCAAAAATGCTACAAAGAAGTATATCATTTTCAAATATGGCTTTATCGTCTTGTTTACTT
  TTACCAGGAGATGCCACTGTCATAACTTCTTCATGGGATAATAATGTCTATTTTTATTCC
  ATAGCATTTGGAAGACGCCAGGACACGTTAATGGGACATGATGATGCTGTTAGTAAGATC
  TGTTGGCATGACAACAGGCTATATTCTGCATCGTGGGACTCTACAGTGAAGGTGTGGTCT
  GGTGTTCCTGCAGAGATGCCAGGCACCAAAAGACACCACTTTGACTTGCTGGCCGAGCTG
  GAACATGATGTCAGTGTAGATACAATCAGTTTAAATGCTGCAAGCACACTGTTAGTTTCC
  GGCACCAAAGAAGGCACAGTGAATATTTGGGACCTCACAACGGCCACCTTAATGCACCAG
  ATTCCATGCCATTCAGGGATTGTATGTGACACTGCTTTTAGCCCAGATAGTCGCCATGTC
  CTCAGCACAGGAACAGATGGCTGTCTTAATGTCATTGATGTGCAGACAGGAATGCTCATC
  TCCTCCATGACATCAGATGAGCCCCAGAGGTGCTTTGTCTGGGATGGAAATTCCGTTTTA
  TCTGGCAGTCAGTCTGGTGAACTGCTCGTTTGGGACCTCCTTGGAGCAAAAATCAGTGAG
  AGAATACAGGGCCACACAGGTGCTGTGACATGTATATGGATGAATGAACAGTGTAGCAGT
  ATCATCACAGGAGGGGAAGACAGACAAATTATATTCTGGAAATTGCAGTATTAA
  
  Clone variation with respect to NM_003580.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003580 unedited
  NTTGTTCGAAATTTGGTATACGACTCACTATAGGGCGGGCCGCGAATCGGCACCAGCCGG
  AGTCCCCACGCGAGGATGCTGCGGTGAGCGCGGCCGGGACGCCGCGTCGGGCTCTCGGCC
  GCCCAGCGGCGCCGCAGGGGAAGCCAGGCCGGCAGCCGCGCGCTCCCCAGCCGGCCACCA
  ATCCCGCCCTCCCCGGCTCCTCCGGACCTCCTCGGCAGGCGGCGGCGGGGCCTGCCTGTG
  CGCTCTGCGCCCGCCCGCGCCTACCCTCCATGGCGTTTATCCGGAAGAAGCAGCAGGAGC
  AGCAGCTGCAGCTCTACTCCAAGGAGAGATTTTCCTTGCTGCTGCTTAACTTGGAGGAGT
  ACTACTTTGAACAGCATAGAGCCAATCACATTTTGCACAAGGGCAGTCACCATGAAAGGA
  AAATCAGAGGCTCCTTAAAAATATGTTCAAAATCGGTGATTTTTGAACCAGATTCAATAT
  CCCAGCCCATCATCAAGATTCCTTTGAGAGACTGTATAAAAATAGGAAAGCATGGAGAAA
  ATGGAGCCAATAGACACTTCACAAAGGCAAAATCTGGGGGTATTTCACTCATTTTCAGTC
  AGGTATATTTCATTAAAGAACATAATGTTGTTGCACCATATAAAATAGAAAGGGGCAAAA
  TGGAATATGTTTTTGAATTGGATGTTCCCGGGAAAGTGGAAGATGTTGTGGAGACGTTGC
  TTCAGCTTCACAGAGCATCCTGCCTTGGACAATGGGTGACCAAACCGNCATGATAACAGC
  TANTTTGCAGTCTCGTTTAGCTAGAACATCATTTGACAAAAACAGTTCCAAAACATTTCT
  GAAAAGCTGCACATGGAATGCANAGCAGNAATGGGTGACGCCTCTGGTGACTAATCCTGG
  ACACGTGTGCATCACGGACACAAACCTGTATTTTCAGNCCCTCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003580 unedited
  TACGGATTCCCGGCTCCAGTGTTTTTAAGATACCAAACAGAATAGCTGTAATGAAATACA
  AACCGTTCCTATACACAGTAAACTTACTAAGAACTGCTAATCCTTCTCCAGGTTCTTAGA
  ATCTGGGTACAGCATTATAGAAAGTAACCACGCTATGAAAACACACACCCAAAACCCGAT
  GGGTGGCAATTGTGATAGATTAGGTCGCCTCCCTCATATTCAGAAGTAGCAGAGAATACT
  GCACATTTTCTTGGAAAATTTCTTAATTGTTCAGTGCTTTCTGACAAGTTTCCCCAGCCC
  AATTTATCTCTTAGCTATTCTCTGCTACTTAGTCCTGTCTTCTGACAATCATCATACGGG
  GACGATCATCTGCCTTACAGTGTAACATGTTTTTCCTAACACAGCCGCATTTTATCTGCC
  CTAAGAGAATAGCAACTAATGGCTTTCAATGAAGTCACCATGATTTAGAAAGTTTAAAAC
  CACAAATTTTCCATGTGGCAAAACTTCTATTACTAACATTGCACATTCACCAGTCCGTTT
  AAAAGTTCGGCTTAAAAATGCGTTAAATAGAGGNCAATTTAATATTCAGGAGAGGAAAAG
  GCCCTTAATACTGCAATTCCCAGAATTTAATCCGCCTGGCTTGCCCTCCTGCGATGATCC
  TGCTACCCTGTTGATTCCTTCCTATTCCTGTTACATGCCCTGGGGGGCCCTGTGTTGCCT
  CGCTGGTTGTTGCTGGTGGGGGGGGCCTTCGGGGCGGGCGCCCAGACTGCCTGCGGTAGA
  AACGGGTTTTTCTCGCCGACGAGGCCCCTCGGGGCTCCCCCGTGGTTTGGGGGCATAACC
  TACCTGTGGCGCGTGGNCGCCTGTGGCCGGGAGTGGGCCTTGGCGTGCCTGGGGCTTTTG
  GGGGCGGTCCGGCGGTGCCCCGCCCGGGGGGCGTCCCGATCCCCGTTGTCCCACGTCTAA
  TTATTATAAAAAAAAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003580
• ORF Size: 2754 bp
• Insert Size: 3670
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_003580.2, NP_003571.2
• RefSeq Size: 3564
• RefSeq ORF: 2754
• Locus ID: 8439
• Domains: Beach, WD40, GRAM
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (1).