Vinculin (VCL) (NM_003373) Human Untagged Clone

CAT#: SC117985

VCL (untagged)-Human vinculin (VCL), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VCL
• Synonyms: CMD1W; CMH15; HEL114; MV; MVCL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117985 sequence for NM_003373 edited (data generated by NextGen Sequencing)
  ATGCCAGTGTTTCATACGCGCACGATCGAGAGCATCCTGGAGCCGGTGGCACAGCAGATC
  TCCCACCTGGTGATAATGCACGAGGAGGGCGAGGTGGACGGCAAAGCCATTCCTGACCTC
  ACCGCGCCCGTGGCCGCCGTGCAGGCGGCCGTCAGCAACCTCGTCCGGGTTGGAAAAGAG
  ACTGTTCAAACCACTGAGGATCAGATTTTGAAGAGAGATATGCCACCAGCATTTATTAAG
  GTTGAGAATGCTTGCACCAAGCTTGTCCAGGCAGCTCAGATGCTTCAGTCAGACCCTTAC
  TCAGTGCCTGCTCGAGATTATCTAATTGATGGGTCAAGGGGCATCCTCTCTGGAACATCA
  GACCTGCTCCTTACCTTCGATGAGGCTGAGGTCCGTAAAATTATTAGAGTTTGCAAAGGA
  ATTTTGGAATATCTTACAGTGGCAGAGGTGGTGGAGACTATGGAAGATTTGGTCACTTAC
  ACAAAGAATCTTGGGCCAGGAATGACTAAGATGGCCAAGATGATTGACGAGAGACAGCAG
  GAGCTCACTCACCAGGAGCACCGAGTGATGTTGGTGAACTCGATGAACACCGTGAAAGAG
  TTGCTGCCAGTTCTCATTTCAGCTATGAAGATTTTTGTAACAACTAAAAACTCAAAAAAC
  CAAGGCATAGAGGAAGCTTTAAAAAATCGCAATTTTACTGTAGAAAAAATGAGTGCTGAA
  ATTAATGAGATAATTCGTGTGTTACAACTCACCTCTTGGGATGAAGATGCCTGGGCCAGC
  AAGGACACTGAAGCCATGAAGAGAGCATTGGCCTCCATAGACTCCAAACTGAACCAGGCC
  AAAGGTTGGCTCCGTGACCCTAGTGCCTCCCCAGGGGATGCTGGTGAGCAGGCCATCAGA
  CAGATCTTAGATGAAGCTGGAAAAGTTGGTGAACTCTGTGCAGGCAAAGAACGCAGGGAG
  ATTCTGGGAACTTGCAAAATGCTAGGGCAGATGACTGATCAAGTGGCTGACCTCCGTGCC
  AGAGGACAAGGATCCTCACCGGTGGCCATGCAGAAAGCTCAGCAGGTATCTCAGGGTCTG
  GATGTGCTCACAGCAAAAGTGGAAAATGCAGCTCGCAAGCTGGAAGCCATGACCAACTCA
  AAGCAGAGCATTGCAAAGAAGATCGATGCTGCTCAGAACTGGCTTGCAGATCCAAATGGT
  GGACCGGAAGGAGAAGAGCAGATTCGAGGTGCTTTGGCTGAAGCTCGGAAAATAGCAGAA
  TTATGTGATGATCCTAAAGAAAGAGATGACATTCTACGTTCCCTTGGGGAAATATCTGCT
  CTGACTTCTAAATTAGCAGATCTACGAAGACAGGGGAAAGGAGATTCTCCAGAGGCTCGA
  GCCTTGGCCAAACAGGTGGCCACGGCCCTGCAGAACCTGCAGACCAAAACCAACCGGGCT
  GTGGCCAACAGCAGACCGGCCAAAGCAGCTGTACACCTTGAGGGCAAGATTGAGCAAGCA
  CAGCGGTGGATTGATAATCCCACAGTGGATGACCGTGGAGTCGGTCAGGCTGCCATCCGG
  GGGCTTGTGGCCGAAGGGCATCGTCTGGCTAATGTTATGATGGGGCCTTATCGGCAAGAT
  CTTCTCGCCAAGTGTGACCGAGTGGACCAGCTGACAGCCCAGCTGGCTGACCTGGCTGCC
  AGAGGGGAAGGGGAGAGTCCTCAGGCACGAGCACTTGCATCTCAGCTCCAAGACTCCTTA
  AAGGATCTAAAAGCTCGGATGCAGGAGGCCATGACTCAGGAAGTGTCAGATGTTTTCAGC
  GATACCACAACTCCCATCAAGCTGTTGGCAGTGGCAGCCACGGCGCCTCCTGATGCGCCT
  AACAGGGAAGAGGTATTTGATGAGAGGGCAGCTAACTTTGAAAACCATTCAGGAAAGCTT
  GGTGCTACGGCCGAGAAGGCGGCTGCGGTTGGTACTGCTAATAAATCAACAGTGGAAGGC
  ATTCAGGCCTCAGTGAAGACGGCCCGAGAACTCACACCCCAGGTGGTCTCGGCTGCTCGT
  ATCTTACTTAGGAACCCTGGAAATCAAGCTGCTTATGAACATTTTGAGACCATGAAGAAC
  CAGTGGATCGATAATGTTGAAAAAATGACAGGGCTGGTGGACGAAGCCATTGATACCAAA
  TCTCTGTTGGATGCTTCAGAAGAAGCAATTAAAAAAGACCTGGACAAGTGCAAGGTAGCT
  ATGGCCAACATTCAGCCTCAGATGCTGGTTGCTGGGGCAACCAGTATTGCTCGTCGGGCC
  AACCGGATCCTGCTGGTGGCTAAGAGGGAGGTGGAGAATTCCGAGGATCCCAAGTTCCGT
  GAGGCTGTGAAAGCTGCCTCTGATGAATTGAGCAAAACCATCTCCCCAATGGTGATGGAT
  GCAAAAGCTGTGGCTGGAAACATTTCCGACCCTGGACTGCAAAAGAGCTTCCTGGACTCA
  GGATATCGGATCCTGGGAGCTGTGGCCAAGGTCAGAGAAGCCTTCCAACCTCAGGAGCCT
  GACTTCCCGCCGCCTCCACCAGACCTTGAACAACTCCGACTAACAGATGAGCTTGCTCCT
  CCCAAACCACCTCTGCCTGAAGGTGAGGTCCCTCCACCTAGGCCTCCACCACCAGAGGAA
  AAGGATGAAGAGTTCCCTGAGCAGAAGGCCGGGGAGGTGATTAACCAGCCAATGATGATG
  GCTGCCAGACAGCTCCATGATGAAGCTCGCAAATGGTCCAGCAAGGGCAATGACATCATT
  GCAGCAGCCAAGCGCATGGCTCTGCTGATGGCTGAGATGTCTCGGCTGGTAAGAGGGGGC
  AGTGGTACCAAGCGGGCACTCATTCAGTGTGCCAAGGACATCGCCAAGGCCTCAGATGAG
  GTGACTCGGTTGGCCAAGGAGGTTGCCAAGCAGTGCACAGATAAACGGATTAGAACCAAC
  CTCTTACAGGTATGTGAGCGAATCCCAACCATAAGCACCCAGCTCAAAATCCTGTCCACA
  GTGAAGGCCACCATGCTGGGCCGGACCAACATCAGTGATGAGGAGTCTGAGCAGGCCACA
  GAGATGCTGGTTCACAATGCCCAGAACCTCATGCAGTCTGTGAAGGAGACTGTGCGGGAA
  GCTGAAGCTGCTTCAATCAAAATTCGAACAGATGCTGGATTTACACTGCGCTGGGTTAGA
  AAGACTCCCTGGTACCAGTAG
  
  Clone variation with respect to NM_003373.3
  2388 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003373 unedited
  AGAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGCTCTTCGCC
  GGTTCCCGGCCCCGTGGATCCTACTTCTCTGTCGCCCGCGGTTCGCCGCCCCGCTCGCCG
  CCGCGATGCCAGTGTTTCATACGCGCACGATCGAGAGCATCCTGGAGCCGGTGGCACAGC
  AGATCTCCCACCTGGTGATAATGCACGAGGAGGGCGAGGTGGACGGCAAAGCCATTCCTG
  ACCTCACCGCGCCCGTGGCCGCCGTGCAGGCGGCCGTCAGCAACCTCGTCCGGGTTGGAA
  AAGAGACTGTTCAAACCACTGAGGATCAGATTTTGAAGAGAGATATGCCACCAGCATTTA
  TTAAGGTTGAGAATGCTTGCACCAAGCTTGTCCAGGCAGCTCAGATGCTTCAGTCAGACC
  CTTACTCAGTGCCTGCTCGAGATTATCTAATTGATGGGTCAAGGGGCATCCTCTCTGGAA
  CATCAGACCTGCTCCTTACCTTCGATGAGGCTGAGGTCCGTAAAATTATTAGAGTTTGCA
  AAGGAATTTTGGAATATCTTACAGTGGCAGAGGTGGTGGAGACTATGGAAGATTTGGTCA
  CTTACACAAAGAATCTTGGGCCAGGAATGACTAAGATGGCCAAGATGATTGACGAGAGAC
  AGCAGGAGCTCACTCACCAGGAGCACCGAGTGATGTTGGTGAACTCGATGAACACCGTGA
  AAGAGTTGCTGCCAGTTCTCATTTCAGCTATGAAGAATTTTTGTTACACTAAAAACTCAN
  AAACCAAGGCATAGAGGAAGCTTTANAAATCGCAATTTTACTGTAGAANAATGAGTGCTG
  AAATAATGAGATATTTCGTGTGTACAACTCACCTCTTGGGATGAAGATGCCTGGNCCAGC
  AAGACACTGTAGCCATGGAGAGAGCATTGGNCTNCATAGACTCCAAACTGGACCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003373 unedited
  CTATGGACCGGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTAATTTGCTT
  CACTGTGTCTCGAGCACTGATATTGGGAGAAAAGCACATCCGGCATAAAGTGTAAACCAG
  TGTCTCAAACCACTGGAAGAACCGGGAGAGCAAACATGATTTTTCTTATTTCCTCTAAGT
  AATCTTTCTTTAGTAAAACAACAAGTGATCTTTGGCATAGATTCATACTTTAAAGGCATT
  AATATTGCATTTATATCAGGCAAGCAACTATACAAATATGCTGAGGGCCTTGAAAATAAT
  CATCCTCATTTTAAAAGAAATAGTGAAAGCCTGAGTGTAAAGGACCAACTTAAGTTGTAC
  ACATTCGATGTTGGGAACTAACACACAGCGATGGGTGGGAAGGAAGGATGTTCAGGCAAG
  GTTCTTACTCCTTTACTCATCTGGTTCTGGCTTTGGGAAAAAATAAGGTTTCATGTGCTG
  GGAAATACTTAGCAGTAATAAGTACCAAAAAGGAAACACTGCCCTCTCATTTTGCCTAGT
  AGGAACTTACTGTGGTGATAAGAAATATGAAACCCATTACTCTCTTGAACCCCATACTTG
  GGAGTAGATGCAGAGAGCTTGGTTGTAGTTGGTTGCAGGGAGGTAGGAATTACAGGAGTA
  GTATTTCCCATTGATTCATCTTCAGCAGCACTAGAATTCTAGTCTGCAAATACCACTCTC
  CCTTAGGCAGGAAGTGTCCTTCAGACAGGGCTGCCTTGCCATTAAACACTCTTNAGGGAC
  AAGCACTCCTCTTTCTTTAGACTGAATTAAAAAGGATCTTTTAGAGTTACAGATGAACAN
  ACACCTACATGTCTTTTAAAATCTTGTTANAAAGGGATGTGGAGGAGGGCTACAAAACCA
  GNATATAGGAGACTGGTNTGGTTAAAATAAAATGCAGCATTTCAGCTCANATGGACCATT
  AGAGCCTAGATGAATGATAGGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003373
• Insert Size: 4840 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003373.3, NP_003364.1
• RefSeq Size: 5443 bp
• RefSeq ORF: 3201 bp
• Locus ID: 7414
• Cytogenetics: 10q22.2
• Domains: Vinculin
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton
• Gene Summary: 'Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks a 3' coding exon, as compared to variant 1. It encodes a shorter isoform (VCL), which is the major talin-binding protein in platelets.