VDAC1 (NM_003374) Human Untagged Clone

CAT#: SC117987

VDAC1 (untagged)-Human voltage-dependent anion channel 1 (VDAC1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VDAC1
• Synonyms: PORIN; VDAC-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117987 sequence for NM_003374 edited (data generated by NextGen Sequencing)
  ATGGCTGTGCCACCCACGTATGCCGATCTTGGCAAATCTGCCAGGGATGTCTTCACCAAG
  GGCTATGGATTTGGCTTAATAAAGCTTGATTTGAAAACAAAATCTGAGAATGGATTGGAA
  TTTACAAGCTCAGGCTCAGCCAACACTGAGACCACCAAAGTGACGGGCAGTCTGGAAACC
  AAGTACAGATGGACTGAGTACGGCCTGACGTTTACAGAGAAATGGAATACCGACAATACA
  CTAGGCACCGAGATTACTGTGGAAGATCAGCTTGCACGTGGACTGAAGCTGACCTTCGAT
  TCATCCTTCTCACCTAACACTGGGAAAAAAAATGCTAAAATCAAGACAGGGTACAAGCGG
  GAGCACATTAACCTGGGCTGCGACATGGATTTCGACATTGCTGGGCCTTCCATCCGGGGT
  GCTCTGGTGCTAGGTTACGAGGGCTGGCTGGCCGGCTACCAGATGAATTTTGAGACTGCA
  AAATCCCGAGTGACCCAGAGCAACTTTGCAGTTGGCTACAAGACTGATGAATTCCAGCTT
  CACACTAATGTGAATGACGGGACAGAGTTTGGCGGCTCCATTTACCAGAAAGTGAACAAG
  AAGTTGGAGACCGCTGTCAATCTTGCCTGGACAGCAGGAAACAGTAACACGCGCTTCGGA
  ATAGCAGCCAAGTATCAGATTGACCCTGACGCCTGCTTCTCGGCTAAAGTGAACAACTCC
  AGCCTGATAGGTTTAGGATACACTCAGACTCTAAAGCCAGGTATTAAACTGACACTGTCA
  GCTCTTCTGGATGGCAAGAACGTCAATGCTGGTGGCCACAAGCTTGGTCTAGGACTGGAA
  TTTCAAGCATAA
  
  Clone variation with respect to NM_003374.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003374 unedited
  GCTGGATATTTGTATACGACTTACTATAGGGCGGCCGCGAATTCGCACGAGGCCCCGCCC
  TTGGCCCAGCCGCTCGCTCGGCTCCGCTCCCTGGCTCGGCTCCCTGCCTCCGCGTCGCAG
  CCCCCGCCGTAGCCGCCTCCGAGCCCGCCGCCACATCCTCTGAGCAGAAGATGGCTGTGC
  CACCCACGTATGCCGATCTTGGCAAATCTGCCAGGGATGTCTTCACCAAGGGCTATGGAT
  TTGGCTTAATAAAGCTTGATTTGAAAACAAAATCTGAGAATGGATTGGAATTTACAAGCT
  CAGGCTCAGCCAACACTGAGACCACCAAAGTGACGGGCAGTCTGGAAACCAAGTACAGAT
  GGACTGAGTACGGCCTGACGTTTACAGAGAAATGGAATACCGACAATACACTAGGCACCG
  AGATTACTGTGGAAGATCAGCTTGCACGTGGACTGAAGCTGACCTTCGATTCATCCTTCT
  CACCTAACACTGGGAAAAAAAATGCTAAAATCAAGACAGGGTACAAGCGGGAGCACATTA
  ACCTGNGCTGCGACATGGATTTCGACATTGCTGGGCCTTCCATCCGGNGTGCTCTGGTGC
  TAGGTTACGAGGGCTGGCTGGCCGGCTACCAGATGAATTTTGAGACTGCAATATCCCGAG
  TGACCCAGAGCAACCTTGCAGTTGTCTACCAGACTGATGAATTCCAGCTTCACACTAATG
  TGAATGACCGGACAGAGTTTGCGGCTCCATTTACCACCAAGTGAACAAGAAGTGGAGACC
  CGTGTAAATCTGCCTGGACAAGCGGAACAGTAACACCCGCTTCGGAATAGCAGCCAGTAT
  CAAATTGACCTTGACGCCTGCTTTTTGGTTAAAGTGAACCACTCCCACCTCGAAAGTTTA
  TGGAACCCTTCAACCTTAAGCCCAGTTTTAACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003374 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGGTTTCAATATTTT
  ATTCAAGTTTTTTAAGTGTTGTTAATTACAGCATTTGAAGGGGAGGATCTAATTCCAACA
  AAATGGAAGACTCTAAAATGTACCCATTAAACTGCTAAAAAACAAATTGAGTGGTGAGAA
  TACAACAGAAGTCCAATTTAGATTCTGAGTGTTGTCACCATGTTGGAATACAATCACACA
  GACACTTCCAAGCTTATAGCTGGAGCTCCTGGAAGCTATTTCATACTCTGGTGCANGGGC
  AAAAAAAAACACAACCCAGAAAGGGATAAAGTCTGAATTATTGGCTTCATCACATCCACC
  TTCTCCACCCAAAAATGGCACAAAAGAAACAGTTACCACACCCTGCAGACCTTTTGGTGT
  AAAAGAGATGATGATGAAACTGGGTGGGAACANGTCATGAAGATCTGTCTAAAAAGTCCA
  TTCANGTGAGTTTGTACACACCATCAAGCAGCGAGCCCTTTATCAATANGGGGTAGGGAC
  CNANGTTCGATTCTAGGAAATCACATTTCATTCATTACTCATATGAATTTACAATGCCTA
  CATATTATCGCTTCCACTGCAGCATTNCTAGATAAAAAGAACCTGGCATCTAAAGGGGGC
  ACAAGTTCTCCCCGATTTTCCACTGAAGGACCTTTTTTGAAATAAGTTCTTTTGACCCTT
  GAAGGTAACATCTTAAACTGAACAACTTTACCTGGAGGGCTACTTTTTAACATTCTTGCA
  TCCACAATTCCACATAAAGGAACCTAAATCTGAGGGNCCTTGCTGCAAAAATTTAAATAA
  ACATTGCCACGTTTCTTTTGCTTGAATTCCACCTTACCAATCTGGGGCCCACATTAACTT
  TTGCCTCAAAAACTGACAGGGCAGTAAACCTGGCTTAAGTCTGAGGTTCCTAACCTTAGG
  CGGGATTGTCCTTTTACGAAAAAGGGTAGGGTAATTGACCTTGTGGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003374
• Insert Size: 1910 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003374.1, NP_003365.1
• RefSeq Size: 1806 bp
• RefSeq ORF: 852 bp
• Locus ID: 7416
• Cytogenetics: 5q31.1
• Domains: Euk_porin
• Protein Families: Ion Channels: Other
• Protein Pathways: Calcium signaling pathway, Huntington's disease, Parkinson's disease
• Gene Summary: 'This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.[provided by RefSeq, Sep 2010]'
  Transcript Variant: This variant (1) encodes the functional protein.