VDAC2 (NM_003375) Human Untagged Clone

CAT#: SC117988

VDAC2 (untagged)-Human voltage-dependent anion channel 2 (VDAC2), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VDAC2
• Synonyms: POR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003375, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGACCCACGGACAGACTTGCGCGCGTCCAATGTGTATTCCTCCATCATATGCTGACCTTGGCAAAG
  CTGCCAGAGATATTTTCAACAAAGGATTTGGTTTTGGGTTGGTGAAACTGGATGTGAAAACAAAGTCTTG
  CAGTGGCGTGGAATTTTCAACGTCCGGTTCATCTAATACAGACACTGGTAAAGTTACTGGGACCTTGGAG
  ACCAAATACAAGTGGTGTGAGTATGGTCTGACTTTCACAGAAAAGTGGAACACTGATAACACTCTGGGAA
  CAGAAATCGCAATTGAAGACCAGATTTGTCAAGGTTTGAAACTGACATTTGATACTACCTTCTCACCAAA
  CACAGGAAAGAAAAGTGGTAAAATCAAGTCTTCTTACAAGAGGGAGTGTATAAACCTTGGTTGTGATGTT
  GACTTTGATTTTGCTGGACCTGCAATCCATGGTTCAGCTGTCTTTGGTTATGAGGGCTGGCTTGCTGGCT
  ACCAGATGACCTTTGACAGTGCCAAATCAAAGCTGACAAGGAATAACTTTGCAGTGGGCTACAGGACTGG
  GGACTTCCAGCTACACACTAATGTCAATGATGGGACAGAATTTGGAGGATCAATTTATCAGAAAGTTTGT
  GAAGATCTTGACACTTCAGTAAACCTTGCTTGGACATCAGGTACCAACTGCACTCGTTTTGGCATTGCAG
  CTAAATATCAGTTGGATCCCACTGCTTCCATTTCTGCAAAAGTCAACAACTCTAGCTTAATTGGAGTAGG
  CTATACTCAGACTCTGAGGCCTGGTGTGAAGCTTACACTCTCTGCTCTGGTAGATGGGAAGAGCATTAAT
  GCTGGAGGCCACAAGGTTGGGCTCGCCCTGGAGTTGGAGGCTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003375 unedited
  TTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAGAGAGAGAGAGAGAGA
  GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAGCT
  GCTGCAGCTGCAGCCCGACCGCGAGCGTGCCAAGCGGCTTCAGCAGCTAGCGGAGCGGTG
  GCGGCGGCCCCCCTCAGGACACCACCAGATTCCCCTCTTCCCGCGGCCTCGCCATGGCGA
  CCCACGGACAGACTTGCGCGCGTCCAATGTGTATTCCTCCATCATATGCTGACCTTGGCA
  AAGCTGCCAGAGATATTTTCAACAAAGGATTTGGTTTTGGGTTGGTGAAACTGGATGTGA
  AAACAAAGTCTTGCAGTGGCGTGGAATTTTCAACGTCCGGTTCATCTAATACAGACACTG
  GTAAAGTTACTGGGACCTTGGAGACCAAATACAAGTGGTGTGAGTATGGTCTGACTTTCA
  CAGAAAAGTGGAACACTGATAACACTCTGGGAACAGAAATCGCAATTGAAGACCAGATTT
  GTCAAGGTTTGAAACTGACATTTGATACTACCTTCTCACCAAACACAGGAAAGAAAAGTG
  GTAAAATCAAGTCTTCTTACAAGAGGGAGTGTATAAACCTTGGTTGTGATGTTGACTTTG
  ATTTTGCTGGACCTGCAATCCATGGTTCAGCTGTCTTTGGTTATGAGGGCTGGCTTGCTG
  GCTACCAGATGACCTTTGACAGTGCCAAATCAAAGCTGACAAGGAATAACTNTGCAGTGG
  GCTACAGGACTGGGGACTTNCAGCTACACACTAATGTCAATGATGGGACAGAATTTGGAG
  GATCAATTTATCAGAAAGTTTGTGAAGATCTTGACACTTCAGTAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003375 unedited
  GGCAACTTTCCAGGTCCAGGNATAGCACTGGGGAGGGGTCACAGGGCTGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGNCGAGNTTTTTTTTTTTTTT
  TTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAGGTTTCATAAAGGTCTATTTC
  ATTATTGGGGGGTAGCGCATTTAACAGTTAAATACATTTAAATAATGTATAGGGGACTGC
  AGGACTGCAGCATTGGTAACTAGATAACCAACTAAAAACCAGCTAACAAAGAACTGTCTA
  AATACTTAAAATACAGCTCTTGTTGAAATCATCCTTTGTTTTGATCATCTTCTTGGGGGA
  AAAAAGCCTGCTGCTGGTCACAATGGAAATATATTAAGGCCAAATCTTCTGATATCCATT
  CCCAAAGGTTTCTTTCAGCTGGATTAAGCCTCCAACTCCAGGGCGAGCCCAACCTTGTGG
  CCTCCAGCATTAATGCTCTTCCCATCTACCAAAGCAGAGAGTGTAAGCTTCACACCAGGC
  CTCAAAGTCTGAGTATAGCCTACTCCAATTAAGCTAGAGTTGTTGACTTTTGCAAAAATG
  GAAGCAGGGGGATCCAACTGATATTTAGCTGCAATGCCAAAACGAGTGCAGTTGGTACCT
  GATGTCCAAGCAAGGTTTACCTGAAGTGTCAAGATCTTCACAAACTTTCTGATAAATTGA
  TCCTCCAAATTCTGTCCCTCATTGACTTAATNGTGAGCTTGGAAGTCCCCAGTCTGTAGC
  CACTGCAAATTATTCCTTGNCAGCTTGATTTGGCCTGCCAAAGTCATCTGGAGCCAGCAG
  CCAGCCTTCATACCAAGACGCTGACCCTGGATTGCAGGTCAGCAAATCAAGTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003375
• Insert Size: 1570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003375.2, NP_003366.2
• RefSeq Size: 1522 bp
• RefSeq ORF: 885 bp
• Locus ID: 7417
• Cytogenetics: 10q22.2
• Domains: Euk_porin
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Calcium signaling pathway, Huntington's disease, Parkinson's disease
• Gene Summary: 'This gene encodes a member of the voltage-dependent anion channel pore-forming family of proteins that are considered the main pathway for metabolite diffusion across the mitochondrial outer membrane. The encoded protein is also thought to be involved in the mitochondrial apoptotic pathway via regulation of BCL2-antagonist/killer 1 protein activity. Pseudogenes have been identified on chromosomes 1, 2, 12 and 21, and alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]'
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate upstream start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 3, and 5 all encode the same isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.