Ezrin (EZR) (NM_003379) Human Untagged Clone

CAT#: SC117991

EZR (untagged)-Human ezrin (EZR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EZR
• Synonyms: CVIL; CVL; HEL-S-105; VIL2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003379 edited
  ATGCCGAAACCAATCAATGTCCGAGTTACCACCATGGATGCAGAGCTGGAGTTTGCAATC
  CAGCCAAATACAACTGGAAAACAGCTTTTTGATCAGGTGGTAAAGACTATCGGCCTCCGG
  GAAGTGTGGTACTTTGGCCTCCACTATGTGGATAATAAAGGATTTCCTACCTGGCTGAAG
  CTGGATAAGAAGGTGTCTGCCCAGGAGGTCAGGAAGGAGAATCCCCTCCAGTTCAAGTTC
  CGGGCCAAGTTCTACCCTGAAGATGTGGCTGAGGAGCTCATCCAGGACATCACCCAGAAA
  CTTTTCTTCCTCCAAGTGAAGGAAGGAATCCTTAGCGATGAGATCTACTGCCCCCCTGAG
  ACTGCCGTGCTCTTGGGGTCCTACGCTGTGCAGGCCAAGTTTGGGGACTACAACAAAGAA
  GTGCACAAGTCTGGGTACCTCAGCTCTGAGCGGCTGATCCCTCAAAGAGTGATGGACCAG
  CACAAACTTACCAGGGACCAGTGGGAGGACCGGATCCAGGTGTGGCATGCGGAACACCGT
  GGGATGCTCAAAGATAATGCTATGTTGGAATACCTGAAGATTGCTCAGGACCTGGAAATG
  TATGGAATCAACTATTTCGAGATAAAAAACAAGAAAGGAACAGACCTTTGGCTTGGAGTT
  GATGCCCTTGGACTGAATATTTATGAGAAAGATGATAAGTTAACCCCAAAGATTGGCTTT
  CCTTGGAGTGAAATCAGGAACATCTCTTTCAATGACAAAAAGTTTGTCATTAAACCCATC
  GACAAGAAGGCACCTGACTTTGTGTTTTATGCCCCACGTCTGAGAATCAACAAGCGGATC
  CTGCAGCTCTGCATGGGCAACCATGAGTTGTATATGCGCCGCAGGAAGCCTGACACCATC
  GAGGTGCAGCAGATGAAGGCCCAGGCCCGGGAGGAGAAGCATCAGAAGCAGCTGGAGCGG
  CAACAGCTGGAAACAGAGAAGAAAAGGAGAGAAACCGTGGAGAGAGAGAAAGAGCAGATG
  ATGCGCGAGAAGGAGGAGTTGATGCTGCGGCTGCAGGACTATGAGGAGAAGACAAAGAAG
  GCAGAGAGAGAGCTCTCGGAGCAGATTCAGAGGGCCCTGCAGCTGGAGGAGGAGAGGAAG
  CGGGCACAGGAGGAGGCCGAGCGCCTAGAGGCTGACCGTATGGCTGCACTGCGGGCTAAG
  GAGGAGCTGGAGAGACAGGCGGTGGATCAGATAAAGAGCCAGGAGCAGCTGGCTGCGGAG
  CTTGCAGAATACACTGCCAAGATTGCCCTCCTGGAAGAGGCGCGGAGGCGCAAGGAGGAT
  GAAGTTGAAGAGTGGCAGCACAGGGCCAAAGAAGCCCAGGATGACCTGGTGAAGACCAAG
  GAGGAGCTGCACCTGGTGATGACAGCACCCCCGCCCCCACCACCCCCCGTGTACGAGCCG
  GTGAGCTACCATGTCCAGGAGAGCTTGCAGGATGAGGGCGCAGAGCCCACGGGCTACAGC
  GCGGAGCTGTCTAGTGAGGGCATCCGGGATGACCGCAATGAGGAGAAGCGCATCACTGAG
  GCAGAGAAGAACGAGCGTGTGCAGCGGCAGCTGCTGACGCTGAGCAGCGAGCTGTCCCAG
  GCCCGAGATGAGAATAAGAGGACCCACAATGACATCATCCACAACGAGAACATGAGGCAA
  GGCCGGGACAAGTACAAGACGCTGCGGCAGATCCGGCAGGGCAACACCAAGCAGCGCATC
  GACGAGTTCGAGGCCCTGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003379 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCTGACTCCAGGTT
  GGGACAGCGTCTTCGCTGCTGCTGGATAGTCGTGTTTTCGGGGATCGAGGATACTCACCA
  GAAACCGAAAATGCCGAAACCAATCAATGTCCGAGTTACCACCATGGATGCAGAGCTGGA
  GTTTGCAATCCAGCCAAATACAACTGGAAAACAGCTTTTTGATCAGGTGGTAAAGACTAT
  CGGCCTCCGGGAAGTGTGGTACTTTGGCCTCCACTATGTGGATAATAAAGGATTTCCTAC
  CTGGCTGAAGCTGGATAAGAAGGTGTCTGCCCAGGAGGTCAGGAAGGAGAATCCCCTCCA
  GTTCAAGTTCCGGGCCAAGTTCTACCCTGAAGATGTGGCTGAGGAGCTCATCCAGGACAT
  CACCCAGAAACTTTTCTTCCTCCAAGTGAAGGAAGGAATCCTTAGCGATGAGATCTACTG
  CCCCCCTGAGACTGCCGTGCTCTTGGGGTCCTACGCTGTGCAGGCCAAGTTTGGGGACTA
  CAACAAAGAAGTGCACAAGTCTGGGTACCTCAGCTCTGAGCGGCTGATCCCTCAAAGAGT
  GATGGACCAGCACAAACTTACCAGGGACCAGTGGGAGGACCGGATCCAGGTGTGGCATGC
  GGAACACCGTGGGATGCTCAAAGATAATGCTATGTTGGAATACCTGAAGATTGCTCANGA
  CCTGNAAATGTATGGAATCAACTATTTCGAGATAAAAAACAAGAAGGAACAGACCTTTGG
  CTTGGNAGTNGATGCCCTTGGACTGAATATTTATGAGAAAGATGATAAGNTAACCCNCAA
  GATTGGCTTTCCTTGGNAGTGAATCANGAACATCTCTTTCAATGACAAAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003379 unedited
  GCACGCAATCTAGAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATAGGAAA
  TTCTTTTATTCTGTAAAAGGTAACAAATTATACAGAACAAAACTTTCCCTTTTTAAAACT
  AATGTTACAAATCTGTATTATCACTTGTATATAAATAGTATATAGCTGATCATTAATAAG
  GGGTATAAGTACAATGTATTCTAAAACTGTTAAGCCAAAAAAAAAAAACTAAAAAAATAT
  CCAAGGGTCCTCCTCCACCACTCACGCTGGGGATCACTGGGCTCTCTGCCACCTGCGTGG
  ATTGACGGGAGGAGGGAATCACTGTGTGTGCAAAAGTGCTTCAAACTCAATTTCCAAAAT
  AATTTTCACCCCTCTAAGCATGTAAATATACAAAGATGGATCCTTCATAAAAATTAAAAA
  ATCAATTGGAGCTCATTTCAAATACAAAACAAGTATGGCACAAATGGAAGTCCTGCCACG
  TTTCCTTTAATGATGCTGACTCTTGTATCACACAGGCCAGCATGAAGTTTCTTACTCAAA
  CTTTACAGGCATTTTCCGTAATTCAATCAGTCCTGCTCCCAGCACAACACAGGAGGTGAT
  TCGAGAATAATCGCGAGATTCAGGCCTGCTTGGCACTATTACAACTGGGGAAAACAAACC
  AGGGCGCCTCCCTGGTTCCCAGCCCAAAATGTTTCTGTTGGGTAACTGCTTTCTAAAGGA
  ACTGGGATCTGAGGGAGTTCCTATACTTGGAGCACTAAAGACAAGCGTGGGCGGGCCTGC
  ATCCGCCCGCTATGACCACCCCTCTGCCCTTGGTCCTGCCCTGGCTGTTACAGGGCCTTA
  ATCTCTCAATGCCCTGTTGGTNGTGCCCTGCCAGATCTTGCGCAAGGTTTGTACTTGTCC
  AGGCCTGCCTATGTTCCGTTGGGATGAGCATGGGGGCCTCTTTTCTTATTGGCCTGGACA
  TCTCGTGTCAAGTAANATTGCCGTGACACCTGTCTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003379
• Insert Size: 2700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003379.3, NP_003370.2
• RefSeq Size: 3166 bp
• RefSeq ORF: 1761 bp
• Locus ID: 7430
• Cytogenetics: 6q25.3
• Domains: B41, ERM
• Protein Families: Druggable Genome
• Protein Pathways: Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
• Gene Summary: 'The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.