WIPF1 (NM_003387) Human Untagged Clone

CAT#: SC117996

WIPF1 (untagged)-Human WAS/WASL interacting protein family, member 1 (WIPF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WIPF1
• Synonyms: PRPL-2; WAS2; WASPIP; WIP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117996 sequence for NM_003387 edited (data generated by NextGen Sequencing)
  ATGCCTGTCCCTCCCCCTCCAGCACCCCCGCCGCCCCCGACGTTTGCACTGGCCAATACA
  GAGAAGCCTACCTTGAATAAGACAGAGCAGGCTGGGAGAAATGCTCTCCTTTCTGATATC
  AGCAAAGGGAAGAAACTAAAGAAGACGGTCACCAATGACAGAAGTGCACCAATACTGGAC
  AAACCTAAAGGAGCTGGTGCTGGAGGCGGTGGTGGTGGCTTTGGTGGAGGCGGCGGATTT
  GGCGGAGGAGGTGGTGGCGGAGGCGGTGGAAGTTTTGGAGGGGGCGGACCTCCAGGTCTG
  GGAGGATTGTTCCAGGCTGGAATGCCGAAGCTGAGATCCACGGCCAACAGGGATAATGAT
  TCTGGAGGAAGCCGACCACCATTGTTGCCACCGGGAGGAAGATCCACATCTGCGAAACCC
  TTTTCACCCCCAAGTGGCCCAGGGAGGTTTCCTGTGCCTTCTCCAGGCCACAGAAGTGGT
  CCCCCAGAGCCTCAGAGGAACCGAATGCCGCCCCCAAGGCCCGACGTGGGCTCAAAGCCT
  GATAGCATTCCTCCTCCAGTACCTAGTACTCCAAGACCCATTCAATCAAGTCTGCACAAC
  CGGGGGTCCCCACCAGTGCCCGGAGGCCCCAGGCAGCCCAGCCCCGGGCCCACTCCTCCC
  CCTTTCCCTGGAAACCGCGGCACTGCTTTGGGAGGAGGCTCAATACGTCAGTCCCCCTTG
  AGCTCCTCCTCGCCCTTCTCCAACCGGCCTCCCCTGCCGCCTACCCCCAGCAGGGCCTTG
  GATGACAAACCCCCTCCACCACCTCCTCCAGTGGGCAACAGGCCCTCCATCCACAGGGAA
  GCGGTTCCCCCTCCTCCTCCTCAGAACAACAAGCCTCCAGTGCCTTCCACTCCGCGGCCT
  TCGGCCTCCTCACAGGCCCCACCTCCGCCGCCACCTCCCAGCAGGCCCGGGCCGCCTCCT
  CTGCCTCCAAGTTCCAGCGGCAATGACGAAACCCCAAGACTCCCACAGCGGAATCTGTCC
  CTCAGTTCGTCCACGCCCCCGTTACCTTCGCCAGGACGTTCAGGTCCTCTTCCTCCCCCG
  CCCAGTGAGAGACCCCCACCTCCAGTGAGGGACCCGCCAGGCCGATCAGGCCCCCTCCCA
  CCACCTCCTCCAGTAAGCAGAAACGGCAGCACATCTCGGGCCCTGCCTGCTACCCCTCAG
  TTGCCATCCAGGAGTGGAGTAGACAGTCCCAGGAGTGGACCCAGGCCTCCCCTTCCTCCT
  GATAGGCCCAGTGCTGGGGCACCTCCCCCACCTCCACCATCAACATCTATTAGAAATGGC
  TTCCAAGACTCTCCATGTGAAGATGAGTGGGAAAGCAGATTCTACTTCCATCCGATTTCC
  GATTTGCCACCTCCAGAGCCATATGTACAAACGACCAAAAGTTATCCCAGCAAACTGGCA
  AGAAACGAAAGCCGGAGTGGATCCAACCGAAGAGAAAGGGGTGCTCCACCACTCCCTCCC
  ATCCCGAGGTGA
  
  Clone variation with respect to NM_003387.4
  593 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003387 unedited
  ACTCCTATAGGCGGCCGCGAATTCGGCACGAGGGTTCCCGGATGGTCGCAGCCTCCCGGC
  GCTGAGCGCTTTTCCTGCCCGCCCGGCTCAAGCCCTGCGGACCCCGGGAGAAGTTTCCCA
  AAAAAATGCCCAGCGCGGCGCGGGGCTGCGGAGTCGTCCGGAGCCGCTGCGCGATTTATC
  AGCAAGACTGTTGAACGCATAACTGCCCAAGAAGCCTGTCCCTCCCCCTCCAGCACCCCC
  GCCGCCCCCGACGTTTGCACTGGCCAATACAGAGAAGCCTACCTTGAATAAGACAGAGCA
  GGCTGGGAGAAATGCTCTCCTTTCTGATATCAGCAAAGGGAAGAAACTAAAGAAGACGGT
  CACCAATGACAGAAGTGCACCAATACTGGACAAACCTAAAGGAGCTGGTGCTGGAGGCGG
  TGGTGGGGTTTGGTGGGGAAAAGGCGGCGGATTTGGCGGAGGAAGGTGGTGGCGGAGGCG
  TNNGGAAGTTTTGGAGGGGGGCGGACCTCCAGGTCTGGGAAGGATTGTTCCAGGCTGGAA
  TGCCGAAGCTGAGATCCACGGCCAACAGGGATAATGATTCTGGAGGAAGCCGACCACCAT
  TGTTGCACCGGGAAGGAAGATCCACATCTGCGAAACCCTTTTCACCCCCAAGTGGCANAG
  GGGGAGGTTTCCTTGTGCCCTTCTCCAGGCACAANAAAAGTGGTCCCCCAGAGCCTCAGA
  AGAAACNGAAATGCCGCCCCAAGGNCCNANACGGGGGGCTCAAAGCCTGAATAGCATTCC
  TCCTCCAGTACCCTAGTACTCCAAGACCCATTCATCAGCTGNNCNNGGGGGGGGGGGTCC
  CCAACATGGCCCGGGGAGGCCCAAAGAAGCCAAACCCGGGCCCAATTCTTCCCCTTTCCT
  GAAAACGGACTGTTTTTTGGGGGGAGAGGGTAAAAAAAAAAGCCCCCTGGGAGTTCTCTT
  GCCTTTTAAACGGGTTCCCTGCCGCTACCCAAAGGGGGTGGGGTGGAAAAACCCTCCAAC
  CTTTCATGGGGAAAGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003387 unedited
  ATGGACCGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTTTTTTACATTCT
  GCTTTTCTTTATTGTCTGGCTAACTTACAAAAATGCAAATGTCTAGGGTAGTCTCTACCC
  TACCACTTACACTATCCTGATGACACAGATAGCAAAATGGGTCTGTTTACATAGGGCATG
  ATATGAAAAAAAAGTTTTTCTTCCTCTACGGTCCTTGACTATAAGGAGGGAAAAATTAAT
  TTCATGCCAACATTTTTGGGGAACTTTAACAATCATCCCATTTCTGCTACTAAAATAACA
  AAACTGGTATTACACTTTAAAATATAAAGACCTAACAGTTTTTACAAATATGCAAATAAT
  CTACTACTTAAACATAAAAAAAAGTTGATTTCTTTTAAATCACAAAGTAAGGCACCATTG
  GATTAAACATTTCTCCTGGCTTTTACTAAATAAAATGCATAGGGAAATAAATACTGAACA
  CTGAGTTTTAATACTGTAATACATTTCAATATAAAATAAGAGGTGAATGTGAAAATACTG
  TATTACATGTTGAATACATTTATCTGAAAATGTTATAAAAAAACACACATGTAAGCTCTG
  ATTTCAGGGAAGAAAAATTCATTTTTGTAATTTTCCATAGTTTAAGATTTTACCACAGAA
  CTTATTCATAGTTTTAGATGCAATTAGGTTGCAAACTTTCAAAGAAAGGGTGTAAGGTGT
  ATTAATGGAAACAGCCACTTAAACACTACATTCTAAAACAATCTATTCTGGATGAATGGC
  ANCTTTGATCTATCACCCCTGTTCAGATTTAAACGGTACCCTGCCAAGTCAGATATGCAA
  GGAATTGTCCAATTCTTACTACCCCTTAAAAATCAGACCACTTTTNTGAGCAAACTTTCT
  CCGCATATTTTTAGAAGGGCAATTCCATCTTGGGAATGGGTCATTAGCTTTATAAAGGAG
  GTTTTACGTGGGAATCACAAAAGATCCCAATAAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003387
• Insert Size: 4570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003387.3, NP_003378.3
• RefSeq Size: 4605 bp
• RefSeq ORF: 1512 bp
• Locus ID: 7456
• Cytogenetics: 2q31.1
• Domains: WH2
• Gene Summary: 'This gene encodes a protein that plays an important role in the organization of the actin cytoskeleton. The encoded protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these two proteins may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein.