WNT8B (NM_003393) Human Untagged Clone

CAT#: SC118001

WNT8B (untagged)-Human wingless-type MMTV integration site family, member 8B (WNT8B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WNT8B
• Synonyms: OTTHUMP00000020285; wingless-type MMTV integration site family, member 8B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003393 edited
  GAATTCGGCACGAGGGAGGATTATGTTTCTTTCAAAGCCTTCTGTGTACATCTGTCTTTT
  CACCTGTGTCCTCCAACTCAGCCACAGCTGGTCGGTGAACAATTTCCTGATGACTGGTCC
  AAAGGCTTACCTGATTTACTCCAGCAGTGTGGCAGCTGGTGCCCAGAGTGGTATTGAAGA
  ATGCAAGTATCAGTTTGCCTGGGACCGCTGGAACTGCCCTGAGAGAGCCCTGCAGCTGTC
  CAGCCATGGTGGGCTTCGCAGTGCCAATCGGGAGACAGCATTTGTGCATGCCATCAGTTC
  TGCTGGAGTCATGTACACCCTGACTAGAAACTGCAGCCTTGGAGATTTTGATAACTGTGG
  CTGTGATGACTCCCGCAACGGGCAACTGGGGGGACAAGGCTGGCTGTGGGGAGGCTGCAG
  TGACAATGTGGGCTTCGGAGAGGCGATTTCCAAGCAGTTTGTCGATGCCCTGGAAACAGG
  ACAGGATGCACGGGCAGCCATGAACCTGCACAACAACGAGGCTGGCCGCAAGGCGGTGAA
  GGGCACCATGAAACGCACGTGCAAGTGCCACGGCGTGTCTGGCAGCTGCACCACGCAGAC
  CTGTTGGCTGCAGCTGCCCGAGTTCCGCGAGGTGGGCGCGCACCTGAAGGAGAAGTACCA
  CGCAGCACTCAAGGTGGACCTGCTGCAGGGTGCTGGCAACAGCGCGGCCGGCCGCGGCGC
  CATCGCCGACACCTTTCGCTCCATCTCTACCCGGGAGCTGGTGCACCTGGAGGACTCCCC
  GGACTACTGCCTGGAGAACAAAACGCTAGGGCTGCTGGGCACCGAAGGCCGAGAGTGCCT
  AAGGCGCGGGCGGGCCCTGGGTCGCTGGGAACGCCGCAGCTGCCGCCGGCTCTGCGGGGA
  CTGCGGGCTGGCGGTGGAGGAGCGCCGGGCCGAGACCGTGTCCAGCTGCAACTGCAAGTT
  CCACTGGTGCTGCGCAGTCCGCTGCGAGCAGTGCCGCCGGAGGGTCACCAAGTACTTCTG
  TAGCCGCGCAGAGCGGCCGCGGGGGGGCGCTGCGCACAAACCCGGGAGAAAACCCTAAGG
  GTTTCCTCTGCCCCCTCCTTTTCCCACTGGTTCTTGGCTTCCTTTAGAGACCCCGGTAAT
  TGTGGAACCTAGGGAATGGGGAACCCGCTCTCCCAGACCTAGGGATCCTGAGAGGGAGAG
  ACTGCAATTTCTCCAAAGCTTGCCACTTTCCAGCCTGTTTCCCCAATTCCTCTGTGCTCT
  CCTAGAGCTCTGTCTGAATCCTCGCAGCCACACCTAGGTCTGAGAACTCAGGCTTTGAGT
  TACTGATCTTCCTTGGATTAGGAGAACAGGTGTTCCTCCTCCCCTCTCCTAGCAGCCCTA
  ATGTCTGACCTAGCCTATCAAGCCTTAGGCGCTGGAAGAACCCTTCTCAGACACGCAGGA
  CCCAGGTAAAGTCAAAGCTTTGCCCTTTTGCCCACTGTCTGCTACCAGGGCTCACCCACT
  GCTGCACCTCTCTTCTGCACAGCTCCTCCCCTGCTACCTGCTGAGCCAGATTCCCCAGGA
  ATCTTGAATGCTTTCTCTCCTCTTCTCCCTTTCCTTTCCCAGAAAAACTGAGGAAACTGG
  CCCCGGAAAAGCATGTCTTTGGGGTTGGTTCCTAGAGGCAGAGGTTGAAGATGGAAGAGG
  GAGCTCTGGAGTGCTAACTTGAACACCAAGGGTGCTACTCATCCCTATGGTATCATATCA
  TGAATGGACTTTACTAGTGGGGCAATGACTTTCCTAGACAATAACCCGAGGGACTCCAGA
  TACATACCCCGAAGGTCTAGGAAATACGTTAAGGGCAGATTACAGTCATTTCCTACCCTT
  TAAAGGTAACTTCTCCCTTCTCCTGACCTACTTCCTCCTAGCAACCAACTTTACCTCTTC
  TTCTCCAAAGGATCTTTGTTCCTCTGAGCCAAGACTGAGGTAAATAAAGCCACTTTCCTC
  TTCAGATCCTGGTCTGCACCTCTAGAGGCAGGTCCTAAGAAGGAGAAACTGAGACCCTTC
  CTTCTCACTAACCTGGTTCTCAAACAGGCAGGATTCAGAGCACTGGATATCTTCCTTCAA
  TGAGTAAACTTCCTGCAATGAAACTGTTGCTTAGTAGGAAGTTCAGCACCTTGAACCTTA
  ATTTATTGCAGTCATTGTGATATGTGCTCTTTTTCTAGGTACTGCACTTATTCTTGAGGA
  TATTCGGGTGGGCTGTGGTAACAGATACAACCTGTAGGCTCTCTCCTCCCTCCCATCTTG
  GCAGGATACCATCCTCCCTAAAGCCTCACTTCCCTGGTGGCCCAAGCAGTTATCTGGATT
  GAGGCTAAGCTGCCAAGGATTAGTAAATCATCTATTTAAATCTTCTAATACATTCACTCA
  AGAGCCATGAGCTTGCTGCTTGTGGTACTTGAGTCTTCTGGTTTTATTCTCAGAGAGTAC
  AGCAGGXXXXXXXXXXXXXXXXXXXXXXXAGCACTCTAAATACGTCATGCCGCCAGAACA
  TTATTTCATAGGTTATTGTTTTATACCCCCAAATGGATACCAGATTCATTCCAGTTCTAC
  CAAGAAAACATGACTGAGATGCCTTGGATCTGTATTGCTTTTCCCTTCCTTTTGCAATAT
  CCACTTGTTCATTTGTTCATTCAACAAACACCAGAGTAGAGATAGTGGTGAACAACCACC
  ATTGCTGCATCACGGAGTTTAAGTTCTCATCTTACACTCAATTGCCAGGGCCGTGGTCCC
  TTTACAAAGTAGACAACTCCAGAACTGGTGCTTGGGAAGACATCTTTGAGGTCAGCCAGT
  CAAACCTTTTGTGTTACAGATTTAAACTGATGCTGAGAAAGATGACTGGCTTCTTCCCCC
  AACTAGTAAGTTACTGTGGGGAGAGTGCAGAGCACAGGCCCCTTCCACCTCTTGTGTGCT
  CTTCAGGAGCCCATCTGTCTCAGCCAGAAGACAGAAACAGAGGGAGGCCCTGAGAAGTGA
  CAAGGACATTGGTTGGGGTGGGCAAAACACTGTCTCCTTCCTGTCCCTGGCCTGTGAGCC
  TAAACACAGGCAGAGCTTTTTCTTTTTGAAAAGAATCGCAGCCTCAACTGGCCTTAGCAG
  CAGGTTAGGGCAAAGACAGAGACGGGGTCGTCTTCGGCGCTGGTGGTGCTGGCGGTGGTG
  GGAGGGGAGGGCAGGCCAGGGCACTCGCCTATTGTAGGTCTGGATTGGATGCTTCCACTC
  ATTCATTTCCTTTTAATTAACCCGCATTCCTGGAGGAGAAATATTTATGTTGGCTCCCAT
  AGAGGCAATCCTCAGACTGGAAAGAAGGGGGAAGAAAGCCTGTCTTAGCAAAACAATATC
  TCTAAAGAGCTCAAGAAGAGAAAATAGGAGAACAAAATGCCATXXXXXXXXXXXXXXAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  CTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003393 unedited
  GTTGCACCATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGGAGGATTA
  TGTTTCTTTCAAAGCCTTCTGTGTACATCTGTCTTTTCACCTGTGTCCTCCAACTCAGCC
  ACAGCTGGTCGGTGAACAATTTCCTGATGACTGGTCCAAAGGCTTACCTGATTTACTCCA
  GCAGTGTGGCAGCTGGTGCCCAGAGTGGTATTGAAGAATGCAAGTATCAGTTTGCCTGGG
  ACCGCTGGAACTGCCCTGAGAGAGCCCTGCAGCTGTCCAGCCATGGTGGGCTTCGCAGTG
  CCAATCGGGAGACAGCATTTGTGCATGCCATCAGTTCTGCTGGAGTCATGTACACCCTGA
  CTAGAAACTGCAGCCTTGGAGATTTTGATAACTGTGGCTGTGATGACTCCCGCAACGGGC
  AACTGGGGGGACAAGGCTGGCTGTGGGGAGGCTGCAGTGACAATGTGGGCTTCGGAGAGG
  CGATTTCCAAGCAGTTTGTCGATGCCCTGGAAACAGGACAGGATGCACGGGCAGCCATGA
  ACCTGCACAACAACGAGGCTGGCCGCAAGGCGGTGAAGGGCACCATGAAACGCACGTGCA
  AGTGCCACGGCGTGTCTGGCAGCTGCACCACGCAGACCTGTTGGCTGCAGCTGCCCGAGT
  TCCGCGAGGTGGGCGCGCACCTGAAGGAGAAGTACCACGCAGCACTCAAGGTGGACCTGC
  TGCANGGTGCTGGCAACAGCGCGGCCGGCCGCGGCGCCATCGCCGACACCTTTCGCTCCA
  TCTCTACCCGGGAGCTGGTGCACCNTGGAGACTCCCCGNACTACTGCCTGNAGAACAAAC
  GCTNAGGCTGCTGGNCACCGAANGCCGANAGTGCTAANGCGCGCCGGCCCTGGTCGCTGG
  AACGCCGCACTGCCCGCGCTCTGCGGGACGCGGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003393 unedited
  GTAGAAGCTTTTTATGGCATTTTGTNTCTCCTATTTTCTCTTCTTGAGCTCTTTAGAGAT
  TTGTTTTGCTAAGACAGGCTTTCTTCCCCCTTCTTTCCAGTCTGAGGATTGCCTCTATGG
  GAGCCAACATAAATATTTCTCCTCCAGGAATGCGGGTTAATTAAAAGGAAATGAATGAGT
  GGAAGCATCCAATCCAGACCTACAATAGGCGAGTGCCCTGGCCTGCCCTCCCCTCCCACC
  ACCGCCAGCACCACCAGCGCCGAAGACGACCCCGTCTCTGTCTTTGCCCTAACCTGCTGC
  TAAGGCCAGTTGAGGCTGCGATTCTTTTCAAAAAGAAAAAGCTCTGCCTGTGTTTAGGCT
  CACAGGCCAGGGACAGGAAGGAGACAGTGTTTTGCCCACCCCAACCAATGTCCTTGTCAC
  TTCTCAGGGCCTCCCTCTGTTTCTGTCTTCTGGCTGAGACAGATGGGCTCCTGAAGAGCA
  CACAAGAGGTGGAAGGGGCCTGTGCTCTGCACTCTCCCCACAGTAACTTACTAGTTGGGG
  GAAGAAGCCAGTCATCTTTCTCAGCATCAGTTTAAATCTGTAACACAAAAGGTTTGACTG
  GCTGACCTCAAAGATGTCTTCCCAAGCACCAGTTCTGGAGTTGTCTACTTTGTAAAGGGA
  CCACGGCCCTGGCAATTGAGTGTAAGATGAGAACTTAAACTCCGTGATGCAGNCATGGGT
  GGTTGTTCACCACTATCTCTACTCTGGNTGTTTGNTGAATGAACANATGAACAAGTGGAT
  ATTGCAAAGGGAAGGNAAAGCATACAGATCCAGGCATCTCAGTCATGTTTTCTTGGTAGA
  CTGGATGAATCTGNTTNCATTTGNGGNNTATAAACATACCTATGAAAATATGTCTGGCGC
  ATGACGATTTANAGGGCTGACTGNAGTACCAATTGAAGCCATAGTCTGCTTTTGCTAGAG
  ACTGAGATGTACTTACTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003393
• Insert Size: 4930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003393.2, NP_003384.1
• RefSeq Size: 2117 bp
• RefSeq ORF: 1056 bp
• Locus ID: 7479
• Cytogenetics: 10q24.31
• Domains: wnt
• Protein Families: Cancer stem cells, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• Gene Summary: 'The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 95%, 86% and 71% amino acid identity to the mouse, zebrafish and Xenopus Wnt8B proteins, respectively. The expression patterns of the human and mouse genes appear identical and are restricted to the developing brain. The chromosomal location of this gene to 10q24 suggests it as a candidate gene for partial epilepsy. [provided by RefSeq, Jul 2008]'