14-3-3 beta (YWHAB) (NM_003404) Human Untagged Clone

CAT#: SC118005

YWHAB (untagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: YWHAB
• Synonyms: GW128; HEL-S-1; HS1; KCIP-1; YWHAA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118005 sequence for NM_003404 edited (data generated by NextGen Sequencing)
  ATGACAATGGATAAAAGTGAGCTGGTACAGAAAGCCAAACTCGCTGAGCAGGCTGAGCGA
  TATGATGATATGGCTGCAGCCATGAAGGCAGTCACAGAACAGGGGCATGAACTCTCCAAC
  GAAGAGAGAAATCTGCTCTCTGTTGCCTACAAGAATGTGGTAGGCGCCCGCCGCTCTTCC
  TGGCGTGTCATCTCCAGCATTGAGCAGAAAACAGAGAGGAATGAGAAGAAGCAGCAGATG
  GGCAAAGAGTACCGTGAGAAGATAGAGGCAGAACTGCAGGACATCTGCAATGATGTTCTG
  GAGCTGTTGGACAAATATCTTATTCCCAATGCTACACAACCAGAAAGTAAGGTGTTCTAC
  TTGAAAATGAAAGGAGATTATTTTAGGTATCTTTCTGAAGTGGCATCTGGAGACAACAAA
  CAAACCACTGTGTCGAACTCCCAGCAGGCTTACCAGGAAGCATTTGAAATTAGTAAGAAA
  GAAATGCAGCCTACACACCCAATTCGTCTTGGTCTGGCACTAAATTTCTCAGTCTTTTAC
  TATGAGATTCTAAACTCTCCTGAAAAGGCCTGTAGCCTGGCAAAAACGGCATTTGATGAA
  GCAATTGCTGAATTGGATACGCTGAATGAAGAGTCTTATAAAGACAGCACTCTGATCATG
  CAGTTACTTAGGGACAATCTCACTCTGTGGACATCGGAAAACCAGGGAGACGAAGGAGAC
  GCTGGGGAGGGAGAGAACTAA
  
  Clone variation with respect to NM_003404.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003404 unedited
  GGCAGGATTTTGTAATACGAACTCCTATAGGGCGGCCGCGNAATTCGCACGAGGCCGGAG
  CCGGGGTAGTCGCCGCCGCCGCCGCCGCTGCAGCCACTGCAGGCACCGCTGCCGCCGCCT
  GAGTAGTGGGCTTAGGAAGGAAGAGGTCATCTCGCTCGGAGCTTCGCTCGGAAGGGTCTT
  TGTTCCCTGCAGCCCTCCCACGGGAATGACAATGGATAAAAGTGAGCTGGTACAGAAAGC
  CAAACTCGCTGAGCAGGCTGAGCGATATGATGATATGGCTGCAGCCATGAAGGCAGTCAC
  AGAACAGGGGCATGAACTCTCCAACGAAGAGAGAAATCTGCTCTCTGTTGCCTACAAGAA
  TGTGGTAGGCGCCCGCCGCTCTTCCTGGCGTGTCATCTCCAGCATTGAGCAGAAAACAGA
  GAGGAATGAGAAGAAGCAGCAGATGGGCAAAGAGTACCGTGAGAAGATAGAGGCAGAACT
  GCAGGACATCTGCAATGATGTTCTGGAGCTGTTGGACAAATATCTTATTCCCAATGCTAC
  ACAACCAGAAAGTAAGGTGTTCTACTTGAAAATGAAAGGAGATTATTTTAGGTATCTTTC
  TGAAGTGGCATCTGGAGACAACANACAAACCACTGTGTCGAACTCCCAGCAGGCTTACCC
  AGGAAGCCATTTGAATTAGTAAAGATAGAAATGCAGCCTACACACCCAATTCGGGCTTGT
  TCTGGCACTAAATTTTTCAATCTTTTACTATGAGATTCTAAACTCTCCTGAAAAGCCCTG
  TTACCTGGCAAAAACGGCATTTGATGAAGCAATCGCTGAATTGGATACCCTGAATGAAAA
  GTCTTATAAAGACAGCCTCTGATCATGCAGTCCCTTAGGGCCACTTCCCTTTGGGGGCCT
  TCGAAAACCAGGAGACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003404 unedited
  NNNTTAGCTCTGCACCGCGCGCCGCATTCTANAGTCGAGTTTTTTTTTTTTTTTTTTGTC
  GCACAAGGGATATATGTTGAGGGTACAGAGTGACACTGAACAGATCACAAAGCACGAGAA
  ACATTAGTTCTCTCCCTCCCCAGCGTCTCCTTCGTCTCCCTGGTTTTCCGATGTCCACAG
  AGTGAGATTGTCCCTAAGTAACTGCATGATCAGAGTGCTGTCTTTATAAGACTCTTCATT
  CAGCGTATCCAATTCAGCAATTGCTTCATCAAATGCCGTTTTTGCCAGGCTACAGGCCTT
  TTCAGGAGAGTTTAGAATCTCATAGTAAAAGACTGAGAAATTTAGTGCCAGACCAAGACG
  AATTGGGTGTGTAGGCTGCATTTCTTTCTTACTAATTTCAAATGCTTCCTGGTAAGCCTG
  CTGGGAGTTCGACACAGTGGTTTGTTTGTTGTCTCCAGATGCCACTTCAGAAAGATACCT
  AAAATAATCTCCTTTCATTTTCAAGTAGAACACCTTACTTTCTGGTTGTGTAGCATTGGG
  AATAAGATATTTGTCCAACAGCTCCAGAACATCATTGCAGATGTCCTGCAGTTCTGCCTC
  TATCTTCTCACGGTACTCTTTGCCCATCTGCTGCTTCTTCTCATTCCTCTCTGTTCTCTG
  CTCAATGCTGGAGATGACCCGCCAGGAAGAGCGCCGGGCGCCCTACACATTCTTGTAAGC
  ACAGAGAGCAAATTCTCCCTTCGTGGGAAGNTCATGCCCCTGGTCTGGGACTGCCTCATG
  GCTGCACCCTTATATCATATCGTTCACCTGCTCAGCGAGTTTGGCTTTCTGAACCACCCC
  ACTTTTACCATTGGCATTCCCGGGGGAGGGCTGAGGGAACAAGAACCTCCCGGAGGAACC
  CCCGACGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003404
• Insert Size: 1000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003404.3, NP_003395.1
• RefSeq Size: 3110 bp
• RefSeq ORF: 741 bp
• Locus ID: 7529
• Cytogenetics: 20q13.12
• Domains: 14-3-3
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis
• Gene Summary: 'This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.