DAP12 (TYROBP) (NM_003332) Human Untagged Clone

CAT#: SC118041

TYROBP (untagged)-Human TYRO protein tyrosine kinase binding protein (TYROBP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TYROBP
• Synonyms: DAP12; KARAP; PLOSL; PLOSL1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118041 sequence for NM_003332 edited (data generated by NextGen Sequencing)
  ATGGGGGGACTTGAACCCTGCAGCAGGCTCCTGCTCCTGCCTCTCCTGCTGGCTGTAAGT
  GGTCTCCGTCCTGTCCAGGCCCAGGCCCAGAGCGATTGCAGTTGCTCTACGGTGAGCCCG
  GGCGTGCTGGCAGGGATCGTGATGGGAGACCTGGTGCTGACAGTGCTCATTGCCCTGGCC
  GTGTACTTCCTGGGCCGGCTGGTCCCTCGGGGGCGAGGGGCTGCGGAGGCAGCGACCCGG
  AAACAGCGTATCACTGAGACCGAGTCGCCTTATCAGGAGCTCCAGGGTCAGAGGTCGGAT
  GTCTACAGCGACCTCAACACACAGAGGCCGTATTACAAATGA
  
  Clone variation with respect to NM_003332.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003332 unedited
  CCCACCGGCCCTTACACTGTGGTGTCCAGCAGCATCCGGCTTCATGGGGGGACTTGAACC
  CTGCAGCAGGCTCCTGCTCCTGCCTCTCCTGCTGGCTGTAAGTGGTCTCCGTCCTGTCCA
  GGCCCAGGCCCAGAGCGATTGCAGTTGCTCTACGGTGAGCCCGGGCGTGCTGGCAGGGAT
  CGTGATGGGAGACCTGGTGCTGACAGTGCTCATTGCCCTGGCCGTGTACTTCCTGGGCCG
  GCTGGTCCCTCGGGGGCGAGGGGCTGCGGAGGCAGCGACCCGGAAACAGCGTATCACTGA
  GACCGAGTCGCCTTATCAGGAGCTCCAGGGTCAGAGGTCGGATGTCTACAGCGACCTCAA
  CACACAGAGGCCGTATTACAAATGAGCCCGAATCATGACAGTCAGCAACATGATACCTGG
  ATCCAGCCATTCCTGAAGCCCACCCTGCACCTCATTCCAACTCCTACCGCGATACAGACC
  CACAGAGTGCCATCCCTGAGAGACCAGACCGCTCCCCAATACTCTCCTAAAATAAACATG
  AAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003332 unedited
  GGGGGGGNNNGGGGGGNNNNNNNNNNTTTTNNNNNNNAANTTTTTACTTAGNACCGNCGN
  CCGCATACTANGATCGNGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTGGGCCCCATGTTTATTTTAAGAAAAAATTGGGGAGCCGGCTG
  GGCTCTCAAGGGAAGGCCCTTTTTGGGGCTTTATCCCGGGAGGAATTGGAAAAAAGGGCC
  GGGGGGGCTTCAAGAAAGGGTGGAACCCAGAATCATGTTGCTGACCGGCAAAAATCCGGC
  CCATTTTTAAAACCGCCCCTTTGGGTTGAGGGCCCTTTAAACATCCCACCCTTGACCCTG
  GAACCCCTGAAAAAGGGAACCGGGCTCAAAGAAACCCTTTTTCCGGGGCCCTTGCTCCCC
  AACCCCTTCCCCCCCAGAGGAACAACCCGCCCCAAAAAAACCCCGCCCAGGCAAAGAACA
  CTTTTAACACCAAGGTTTCCATTAAAAACCCTGGCAAAACCCCCGGGGTTACCCTAAAAC
  AACTGCAATCCCTTTTGGCCCGGGCCCGGACAGGACCGAAACCCCTTTCCCCCCCCCGGA
  AAAGCCGGAACCCGAGCCCTCTTCAAGGGTCAAAACCCCCCCTGAAACCCGAAGCTGCTG
  GACACCCCAATTTAAAGGCCCGTGGGAATTGGCCTTGTGCCCAATTCCCGGCCCGCCTAT
  AATGAGGCCTATTACAAAATTCTTACGGGTCACTTAACGAACTCTGCTTATATAAAACTT
  CCACCGTGAACGCCTTACCGCCATTTTGGTAACGGGGCGGGGGTATTACCACATTTTGGA
  AAGTCCCCTGATTTTGGTGGCCAAACAAATTCCCATTGGCGTAAAGGGGGGGAGACCTTG
  GAATCCCCCGGAGTAAAACGCTATTCCCCCCCTTGGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003332
• Insert Size: 630 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003332.2, NP_003323.1
• RefSeq Size: 583 bp
• RefSeq ORF: 342 bp
• Locus ID: 7305
• Cytogenetics: 19q13.12
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Natural killer cell mediated cytotoxicity
• Gene Summary: 'This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Multiple alternative transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Mar 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).