UBE2D3 (NM_003340) Human Untagged Clone

CAT#: SC118047

UBE2D3 (untagged)-Human ubiquitin-conjugating enzyme E2D 3 (UBE2D3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2D3
• Synonyms: E2(17)KB3; UBC4/5; UBCH5C
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118047 sequence for NM_003340 edited (data generated by NextGen Sequencing)
  ATGGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCCTCCAGCACAA
  TGTTCTGCAGGTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATGGGACCT
  AATGACAGCCCATATCAAGGCGGTGTATTCTTTTTGACAATTCATTTTCCTACAGACTAC
  CCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGT
  AATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGTCGCCTGCTTTAACAATTTCT
  AAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGACCCCCTAGTG
  CCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGTACAACAGAATATCTCGGGAA
  TGGACTCAGAAGTATGCCATGTGA
  
  Clone variation with respect to NM_003340.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003340 unedited
  GTAATACGACTTTCTATAGGGCGGCCGCGAATTCGGCACGAGGGTTTAGTAAGATTGTGC
  TGTTCCGAGAAGGAGAAGGAAAAGGGCTTGACACGTATTCACTCGGCCCCGGACGTGGGA
  AGCAAGCCGTCTGGCTTCGGCCTCACATCGGTCCTGTGCTCGGGACGGCGGCGTTGGCGG
  ACTGATCCGCGGCGGTGAAGAGGCGCCTGTGTCTGGCAGAGCTGGTGTGAGACGAGACAA
  TCCTGCCCCGCCGCCGGGATAATCAAGAGTTTTGGCCGGACCTTTGAGCATACACCGAGA
  GAGTGAGGAGCCAGACGACAAGCACACACTATGGCGCTGAAACGGATTAATAAGGAACTT
  AGTGATTTGGCCCGTGACCCTCCAGCACAATGTTCTGCAGGTCCAGTTGGGGATGATATG
  TTTCATTGGCAAGCCACAATTATGGGACCTAATGACAGCCCATATCAAGGCGGTGTATTC
  TTTTTGACAATTCATTTTCCTACAGACTACCCCTTCAAACCACCTAAGGTTGCATTTACA
  ACAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTCGATATTCTAAGA
  TCACAGTGGTCGCCTGCTNTAACAATTTCTAAAGTTCTTTTATCCATTTGTTCACTGCTA
  TGTGATCCAAACCCAGATGACCCCCTAGTGCCAGAGATTGCACGGATCTATTAAACAGAC
  AGAGATAAGTACCACAGAATATCTCGGGAATGGACTCANNAGTATGCCATGTGATGCTAC
  CTTAAGTCAGNAATACCTGCATATAGCTGGNATAAACTTTAATACTGGTCCTTTNNTGAT
  TNTCTATCCGGCTGCTCCCTATCAGACTCATCTTTNTAAATNTATTTTTGGTTACCTCCT
  CCATTCATCCATGCTATTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003340 unedited
  CCTTCAGCTTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCCAT
  AGCATTGACTTTTATTCATTCATGTTAAGTCACCTGAAATGATATCTGTTGCATTTAAAT
  GCTCATTAATGTAAATGGCTGAACAAATCCATTTCAATGGATACTCAATATCCCACATAG
  ATAAGTCACTTCAACACAGCCTCATGACAACTCCCACACCAAAACAGTACTTTATTTTGC
  AAATTTTGACCCATTATTACTCCCATGTTGTTTTTTTTCAGCTGTCAAGTGTACAAGGTG
  AAGAGAAATTTCAGTTAGACAGGGGCTGCACCTGGAAAATAAATTTCTTAAACTCCATAA
  ACACATTTCAAAACTGCTGGGTCCCAAAAGTCCATCTATGAACTCTAGGGCTGCCAGTAT
  CATGTGCAGCACATTAAAGCTACACTATCCAAATAGCTTATTAATTCTGCATAAATCAGG
  TCAAGCAATGTGTCAACCTGTAACAGACTGTGCACTTTAACTGAACATGGCAAAATATTC
  ACTCTTTCGAACTTTACATCATCATTTGATGTCAAACAATGAAGCAAATCCCAACAGTAT
  ATACAAAAAACAGCTTTGCATTTACAAAAGATTGTTTCCCATACTGATTAATCCCAGCAG
  CTAACTCATTGGTTTATGCAACTTTATTGAAGAAAAATAAATCAATTACTAGCAGAGCTA
  TTAGTTGATCACTCATCCATTGACAACTTGCATCATTTATTCAGTGCTATATTAAACCAG
  TGTATTGGAAAGAAATATTAACTAATAGCCTCCAAGCCTCCTAAACATTTTAAATGGAAA
  ATTCCAAATTGTTTGGGAACCTTATTCTGGGATACAAAAGGGTGTTTGACCTCCAATTTC
  CATCCTTCTGAAAAACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003340
• Insert Size: 2090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003340.4, NP_003331.1
• RefSeq Size: 2386 bp
• RefSeq ORF: 444 bp
• Locus ID: 7323
• Cytogenetics: 4q24
• Domains: UBCc
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. [provided by RefSeq, Jan 2017]'
  Transcript Variant: This variant (1) encodes the predominant isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.