UCP2 (NM_003355) Human Untagged Clone

CAT#: SC118051

UCP2 (untagged)-Human uncoupling protein 2 (mitochondrial, proton carrier) (UCP2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UCP2
• Synonyms: BMIQ4; SLC25A8; UCPH
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118051 sequence for NM_003355 edited (data generated by NextGen Sequencing)
  ATGGTTGGGTTCAAGGCCACAGATGTGCCCCCTACTGCCACTGTGAAGTTTCTTGGGGCT
  GGCACAGCTGCCTGCATCGCAGATCTCATCACCTTTCCTCTGGATACTGCTAAAGTCCGG
  TTACAGATCCAAGGAGAAAGTCAGGGGCCAGTGCGCGCTACAGCCAGCGCCCAGTACCGC
  GGTGTGATGGGCACCATTCTGACCATGGTGCGTACTGAGGGCCCCCGAAGCCTCTACAAT
  GGGCTGGTTGCCGGCCTGCAGCGCCAAATGAGCTTTGCCTCTGTCCGCATCGGCCTGTAT
  GATTCTGTCAAACAGTTCTACACCAAGGGCTCTGAGCATGCCAGCATTGGGAGCCGCCTC
  CTAGCAGGCAGCACCACAGGTGCCCTGGCTGTGGCTGTGGCCCAGCCCACGGATGTGGTA
  AAGGTCCGATTCCAAGCTCAGGCCCGGGCTGGAGGTGGTCGGAGATACCAAAGCACCGTC
  AATGCCTACAAGACCATTGCCCGAGAGGAAGGGTTCCGGGGCCTCTGGAAAGGGACCTCT
  CCCAATGTTGCTCGTAATGCCATTGTCAACTGTGCTGAGCTGGTGACCTATGACCTCATC
  AAGGATGCCCTCCTGAAAGCCAACCTCATGACAGATGACCTCCCTTGCCACTTCACTTCT
  GCCTTTGGGGCAGGCTTCTGCACCACTGTCATCGCCTCCCCTGTAGACGTGGTCAAGACG
  AGATACATGAACTCTGCCCTGGGCCAGTACAGTAGCGCTGGCCACTGTGCCCTTACCATG
  CTCCAGAAGGAGGGGCCCCGAGCCTTCTACAAAGGGTTCATGCCCTCCTTTCTCCGCTTG
  GGTTCCTGGAACGTGGTGATGTTCGTCACCTATGAGCAGCTGAAACGAGCCCTCATGGCT
  GCCTGCACTTCCCGAGAGGCTCCCTTCTGA
  
  Clone variation with respect to NM_003355.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003355 unedited
  ACCTTGGATTTGTATACGACTTTCTATAGGCGGCACGCGAATTCGCACGAGGGATTGACT
  GTCCACGCTCGCCCGGCTCGTCCGACGCGCCCTCCGCCAGCCGACAGACACAGCCGCACG
  CACTGCCGTGTTCTCCCTGCGGCTCGGACACATAGTATGACCATTAGGTGTTTCGTCTCC
  CACCCATTTTCTATGGAAAACCAAGGGGATCGGGCCATGATAGCCACTGGCAGCTTTGAA
  GAATGGGACACCTTTAGAGAAGCTTGATCTTGGAGGCCTCACCGTGAGACCTTACAAAGC
  CGGATTCCGGCAGAGTTCCTCTATCTCGTCTTGTTGCTGATTAAAGGTGCCCCTGTCTCC
  AGTTTTTCTCCATCTCCTGGGACGTAGCAGGAAATCAGCATCATGGTTGGGTTCAAGGCC
  ACAGATGTGCCCCCTACTGCCACTGTGAAGTTTCTTGGGGCTGGCACAGCTGCCTGCATC
  GCAGATCTCATCACCTTTCCTCTGGATACTGCTAAAGTCCGGTTACAGATCCAAGGAGAA
  AGTCAGGGGCCAGTGCGCGCTACAGCCAGCGCCCAGTACCGCGGTGTGATGGGCACCATT
  CTGACCATGGTGCGTACTGAGGGCCCCCGAAGCCTCTACAATGGGCTGGTTGCCGGCCTG
  CAGCGCCAAATGAGCTTTGCCTCTGTCCGCATCGGCCTGTATGATTCTGTCAAACAGTTC
  TACACCAAGGGCTCTGAGCATGCCAGCATTGAGAGCCGCCTCCTAGCATGCAGCACCACA
  GGTGCCCTGGCTGTGGCTGTGGCCCAGCCACGGATGTGGTAAAAGTCCGATTCCAGCTCN
  AGCCNAGGCTGNNAAGTGGTCGAGATACCAAGCACCGTCAATGCCCTACAGACCATTGCC
  CGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003355 unedited
  TTTGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA
  AGCTCAAAAGAGGAGGAGACGCCAAGGTTGAGCTTGCTTTATGGATGACAAGTGGGCTAG
  GCTGGGCTGTCGGGGGCAGGACGAAAATTCTGGCTGAACTTTCCAAGGGACGGGACGCTT
  GGGATCCTGGCTGGTACGAGGCCTCCCACACTGTCAAATGTCAACTCCACCAGCACTGAG
  ACAATGAGTAGATGAGAATGTAAAAAGAGGGAAGGTGGTAGGTAAAGGAGCGGAAGGAAG
  AGGTGGGGAAAGAGGGAAGGAGAGAAGGGAAGGAGGGAAGAGAAAGAAGGAAGAAAAGGA
  AAGCATGGCCCGGCTAGAGACAAAGCCAGAGGTGATCAGGTCAGCAGCAGGAGAGGCTCA
  GAAGGGAGCCTCTCGGGAAGTGCAGGCAGCCATGAGGGCTCGTTTCAGCTGCTCATAGGT
  GACGAACATCACCACGTTCCAGGAACCCAAGCGGAGAAAGGAGGGCATGAACCCTTTGTA
  GAAGGCTCGGCGCCCCTCCTTCTGGAGCATGGAAAGGGCACAGTGGCCAGCGCTACTGTA
  CTGGCCCCAGGCAGAGTTCATGTATCTCGTCTTGACCACGTCTACCGGGGAGGCGATGAC
  AGTGGTGCACAAGCCCGCCCCAGAGCCGCAGTGAAGTGCCAAGGGACGTCATTTGTCACG
  AGGTCGGCTTCCAGGAGGGCATCCCTTGAGAGGCCATTGGTCACCACCTTAGCTCAGTTG
  ACACTGGCCTTCCGACCACCCTGGGAGAAGGCCCCTTTCCAAGAGCACCCGAACCCCTAC
  TTTCGGACCATGGACCCGTAGGCTTGGACGGGCCTATGCACCTTACACCACTCCCCCCCG
  GGCCTGGACTGGCAACGGCCTCTCACCCTTCGGGCCTGGGATAACCCCCAATCGGCACCT
  TGGGGCTTCTGCTCGAGGCGCTCCCACGTGGCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003355
• Insert Size: 1660 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003355.2, NP_003346.2
• RefSeq Size: 1646 bp
• RefSeq ORF: 930 bp
• Locus ID: 7351
• Cytogenetics: 11q13.4
• Domains: mito_carr
• Protein Families: Druggable Genome
• Gene Summary: 'Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'. [provided by RefSeq, Jul 2008]'