TGF beta 2 (TGFB2) (NM_003238) Human Untagged Clone

CAT#: SC118070

TGFB2 (untagged)-Human transforming growth factor, beta 2 (TGFB2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TGFB2
• Synonyms: G-TSF; LDS4; TGF-beta2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003238 edited
  GAATTCGGCACGAGGGTCTCTTTTTTTCCCCATCTCATTGCTCCAAGAATTTTTTTCTTC
  TTACTCGCCAAAGTCAGGGTTCCCTCTGCCCGTCCCGTATTAATATTTCCACTTTTGGAA
  CTACTGGCCTTTTCTTTTTAAAGGAATTCAAGCAGGATACGTTTTTCTGTTGGGCATTGA
  CTAGATTGTTTGCAAAAGTTTCGCATCAAAAACAAACAACAACAACAAAAAACCAAACAA
  CTCTCCTTGATCTATACTTTGAGAATTGTTGATTTCTTTTTTTTATTCTGACTTTTAAAA
  ACAACTTTTTTTTCCACTTTTTTAAAAAATGCACTACTGTGTGCTGAGCGCTTTTCTGAT
  CCTGCATCTGGTCACGGTCGCGCTCAGCCTGTCTACCTGCAGCACACTCGATATGGACCA
  GTTCATGCGCAAGAGGATCGAGGCGATCCGCGGGCAGATCCTGAGCAAGCTGAAGCTCAC
  CAGTCCCCCAGAAGACTATCCTGAGCCCGAGGAAGTCCCCCCGGAGGTGATTTCCATCTA
  CAACAGCACCAGGGACTTGCTCCAGGAGAAGGCGAGCCGGAGGGCGGCCGCCTGCGAGCG
  CGAGAGGAGCGACGAAGAGTACTACGCCAAGGAGGTTTACAAAATAGACATGCCGCCCTT
  CTTCCCCTCCGAAAATGCCATCCCGCCCACTTTCTACAGACCCTACTTCAGAATTGTTCG
  ATTTGACGTCTCAGCAATGGAGAAGAATGCTTCCAATTTGGTGAAAGCAGAGTTCAGAGT
  CTTTCGTTTGCAGAACCCAAAAGCCAGAGTGCCTGAACAACGGATTGAGCTATATCAGAT
  TCTCAAGTCCAAAGATTTAACATCTCCAACCCAGCGCTACATCGACAGCAAAGTTGTGAA
  AACAAGAGCAGAAGGCGAATGGCTCTCCTTCGATGTAACTGATGCTGTTCATGAATGGCT
  TCACCATAAAGACAGGAACCTGGGATTTAAAATAAGCTTACACTGTCCCTGCTGCACTTT
  TGTACCATCTAATAATTACATCATCCCAAATAAAAGTGAAGAACTAGAAGCAAGATTTGC
  AGGTATTGATGGCACCTCCACATATACCAGTGGTGATCAGAAAACTATAAAGTCCACTAG
  GAAAAAAAACAGTGGGAAGACCCCACATCTCCTGCTAATGTTATTGCCCTCCTACAGACT
  TGAGTCACAACAGACCAACCGGCGGAAGAAGCGTGCTTTGGATGCGGCCTATTGCTTTAG
  AAATGTGCAGGATAATTGCTGCCTACGTCCACTTTACATTGATTTCAAGAGGGATCTAGG
  GTGGAAATGGATACACGAACCCAAAGGGTACAATGCCAACTTCTGTGCTGGAGCATGCCC
  GTATTTATGGAGTTCAGACACTCAGCACAGCAGGGTCCTGAGCTTATATAATACCATAAA
  TCCAGAAGCATCTGCTTCTCCTTGCTGCGTGTCCCAAGATTTAGAACCTCTAACCATTCT
  CTACTACATTGGCAAAACACCCAAGATTGAACAGCTTTCTAATATGATTGTAAAGTCTTG
  CAAATGCAGCTAAAATTCTTGGAAAAGTGGCAAGACCAAAATGACAATGATGATGATAAT
  GATGATGACGACGACAACGATGATGCTTGTAACAAGAAAACATAAGAGAGCCTTGGTTCA
  TCAGTGTTAAAAAATTTTTGAAAAGGCGGTACTAGTTCAGACACTTTGGAAGTTTGTGTT
  CTGTTTGTTAAAACTGGCATCTGACACAAAAAATGTTGAAGGCCTTATTCTACATTTCAC
  CTACTTTGTAAGTGAGAGAGACAAGAAGCAAATTTTTTTTAAAGAAAAAAATAAACACTG
  GAAGAATTTATTAGTGTTAATTATGTGAACAACGACAACAACAACAACAACAACAAACAG
  GAAAATCCCATTAAGTGGAGTTGCTGTACGTACCGTTCCTATCCCGCGCCTCACTTGATT
  TTTCTGTATTGCTATGCAATAGGCACCCTTCCCATTCTTACTCTTAGAGTTAACAGTGAG
  TTATTTATTGTGTGTTACTATATAATGAACGTTTCATTGCCCTTGGAAAATAAAACAGGT
  GTATAAAGTGGAGACCAAATACTTTGCCAGAAACTCATGGATGGCTTAAGGAACTTGAAC
  TCAAACGAGCCAGAAAAAAAGAGGTCATATTAATGGGATGAAAACCCAAGTGAGTTATTA
  TATGACCGAGAAAGTCTGCATTAAGATAAAGACCCTGAAAACACATGTTATGTATCAGCT
  GCCTAAGGAAGCTTCTTGTAAGGTCCAAAAACTAAAAAGCCTGTTAATAAAAGAAACTTT
  CAGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003238 unedited
  TCAGAATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGTCTCTT
  TTTTTCCCCATCTCATTGCTCCAANAATTTTTTTCTTCTTACTCGCCAAAGTCAGGGTTC
  CCTCTGCCCGTCCCGTATTAATATTTCCACTTTTGGAACTACTGGCCTTTTCTTTTTAAA
  GGAATTCAAGCAGGATACGTTTTTCTGTTGGGCATTGACTAGATTGTTTGCAAAAGTTTC
  GCATCAAAAACAAACAACAACAACAAAAAACCAAACAACTCTCCTTGATCTATACTTTGA
  GAATTGTTGATTTCTTTTTTTTATTCTGACTTTTAAAAACAACTTTTTTTTCCACTTTTT
  TAAAAAATGCACTACTGTGTGCTGAGCGCTTTTCTGATCCTGCATCTGGTCACGGTCGCG
  CTCAGCCTGTCTACCTGCAGCACACTCGATATGGACCAGTTCATGCGCAAGAGGATCGAG
  GCGATCCGCGGGCAGATCCTGAGCAAGCTGAAGCTCACCAGTCCCCCAGAAGACTATCCT
  GAGCCCGAGGAAGTCCCCCCGGAGGTGATTTCCATCTACAACAGCACCAGGGACTTGCTC
  CAGGAGAAGGCGAGCCGGAGGGCGGCCGCCTGCGAGCGCGAGAGGAGCGACGAAGAGTAC
  TACGCCAAGGAGGTTTACAAAATAGACATGCCGCCCTTCTTCCCCTCCGAAAATGCCATC
  CCGCCCACTTTCTACAGACCCTACTTCAGAATTGTTCGATTTGACGTCTCAGCAATGGAG
  AAAGATGCTTNNCAATTNGTGAAAGCAGAGTTCAGAAGTCTTTCGTTTGCAGAAACCCAA
  AGCCAGAGTGCCCTGACAACGNNATGAGCTATATCAGATTCTCAAGTCCAAAAGATTACA
  TNTNCAACCAGCGCTCATNGCAGNNAAGTGTGAAAACAGACAGAAGCGATGGCTTCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003238 unedited
  GAACCGGCGGCCGCAATCTAGGATCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTCTGACTGAAAGTTTCTTTTATTAACAGGCTTTTTAGTTTTTGGACCTTACAAAAAG
  CTTCCTTAGGCAGCTGATACATAACATGTGTTTTCAGGGTCTTTATCTTAATGCAAACTT
  TCTCGGTCATATAATAACTCACTTGGGTTTTCATCCCATTAATATGACCTCTTTTTTTCT
  GGCTCGTTTGAGTTCAAGTTCCTTAAGCCATCCATGAGTTTCTGGCAAAGTATTTGGTCT
  CCACTTTATACACCTGTTTTATTTTCCAAGGGCAATGAAACGTTCATTATATAGTAACAC
  ACAATAAATAACTCACTGTTAACTCTAAAAGTAAAAATGGGAAGGGTGCCTATTGCATAG
  CAATACAGAAAAATCAAGTGAGGCGCGGGATAGGAACGGTACGTACAGCAACTCCACTTA
  ATGGGATTTTCCTGTTTGTTGTTGTTGCTGTTGTTGCCGTTGTTCACATAATTAACACTA
  ATAAATTCTTCCAGTGTTTATTTTTTTCTTTAAAAAAAATTTGCTTCTTGTCTCTCTCAC
  TTACAAAGTAGGTGAAATGTACAATAAGGCCTTTAACATTTTTTTGTGTCAGACGCCAGT
  TTTACCAAACAGAACACAAACTTCCAAAGTGTCTGAACTAGTACCGCCTTTTCAAAAATT
  TCTTACACCTGAGAACCAAGCTCTCTATGTTTTCTTGTTACAAGCACATCGGTGCCGGCC
  GCCTCATCATAATCATATCATTGCCATTTTGCCCTTGCCACTTTTCCAAGAATTTTAGCT
  GCTTTTGCAAGACTTCCAATCATATAGAAAAGCTGTTCATCCTTGGTGTTTTGCCATGTA
  CTAAAAATGGTAGAGGTTTAATCTTGGCCCGCACAAGAAAACCAAGCTTTGGATTATGAT
  TTAATACCCAAGACCTGTGTGCCGAAGTTGAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003238
• Insert Size: 2290 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003238.1, NP_003229.1
• RefSeq Size: 1695 bp
• RefSeq ORF: 1245 bp
• Locus ID: 7042
• Cytogenetics: 1q41
• Domains: TGFb_propeptide, TGF-beta
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, TGF-beta signaling pathway
• Gene Summary: 'This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers. A chromosomal translocation that includes this gene is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. Mutations in this gene may be associated with Loeys-Dietz syndrome. This gene encodes multiple isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016]'
  Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.