TIMP1 (NM_003254) Human Untagged Clone

CAT#: SC118082

TIMP1 (untagged)-Human TIMP metallopeptidase inhibitor 1 (TIMP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TIMP1
• Synonyms: CLGI; EPA; EPO; HCI; TIMP; TIMP-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118082 sequence for NM_003254 edited (data generated by NextGen Sequencing)
  ATGGCCCCCTTTGAGCCCCTGGCTTCTGGCATCCTGTTGTTGCTGTGGCTGATAGCCCCC
  AGCAGGGCCTGCACCTGTGTCCCACCCCACCCACAGACGGCCTTCTGCAATTCCGACCTC
  GTCATCAGGGCCAAGTTCGTGGGGACACCAGAAGTCAACCAGACCACCTTATACCAGCGT
  TATGAGATCAAGATGACCAAGATGTATAAAGGGTTCCAAGCCTTAGGGGATGCCGCTGAC
  ATCCGGTTCGTCTACACCCCCGCCATGGAGAGTGTCTGCGGATACTTCCACAGGTCCCAC
  AACCGCAGCGAGGAGTTTCTCATTGCTGGAAAACTGCAGGATGGACTCTTGCACATCACT
  ACCTGCAGTTTCGTGGCTCCCTGGAACAGCCTGAGCTTAGCTCAGCGCCGGGGCTTCACC
  AAGACCTACACTGTTGGCTGTGAGGAATGCACAGTGTTTCCCTGTTTATCCATCCCCTGC
  AAACTGCAGAGTGGCACTCATTGCTTGTGGACGGACCAGCTCCTCCAAGGCTCTGAAAAG
  GGCTTCCAGTCCCGTCACCTTGCCTGCCTGCCTCGGGAGCCAGGGCTGTGCACCTGGCAG
  TCCCTGCGGTCCCAGATAGCCTGA
  
  Clone variation with respect to NM_003254.2
  372 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003254 unedited
  GGTAACATCTTGGATTTTGTAATCACGACTTACTATNAGGGCGGCACGCGCAATTCGGCA
  CGAGGCCCAGCGCCCAGAANAAACACCAGAANAACCCACCATGGCCCCCTTTGAGCCCCT
  GGCTTCTGGCATCCTGTTGTTGCTGTGGCTGATAGCCCCCAGCAGGGCCTGCACCTGTGT
  CCCACCCCACCCACAGACGGCCTTCTGCAATTCCGACCTCGTCATCAGGGCCAAGTTCGT
  GGGGACACCAGAAGTCAACCAGACCACCTTATACCAGCGTTATGAGATCAAGATGACCAA
  GATGTATAAAGGGTTCCAAGCCTTAGGGGATGCCGCTGACATCCGGTTCGTCTACACCCC
  CGCCATGGAGAGTGTCTGCGGATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCT
  CATTGCTGGAAAACTGCAGGATGGACTCTTGCACATCACTACCTGCAGTTTCGTGGCTCC
  CTGGAACAGCCTGAGCTTAGCTCAGCGCCGGGGCTTCACCAAGACCTACACTGTTGGCTG
  TGAGGAATGCACAGTGTTTCCCTGTTTATCCATCCCCTGCAAACTGCAGAGTGGCACTCA
  TTGCTTGTGGACGGACCAGCTCCTCCAAGGCTCTGAAAAGGGCTTCCAGTCCCGTCACCT
  TGCCTGCCTGCCTCGGGAGCCAGGGCTGTGCACCTGGCAGTCCCTGCGGTCCCAGATAGC
  CTGAATCCTGCCCGGAGTGGAAGCTGAAGCCTGCACAGTGTCCACCCTGTTCCCACTCCC
  ATCTTTCTTNCCCGGACAATGAATANAGAGTTACCACCCAGCAAAAAAAAAAAAAAAAAA
  CTCGACTCTAGATTGCGGCCCGCGTCATAGCTGTTTCCTGAACAGACCCNGGNTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003254 unedited
  CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTGCTGGGTGGTAACTCTTTATTTC
  ATTGTCCGGAAGAAAGAGGGAGTGGGAACAGGGTGGACACTGTGCAGGCTTCAGCTTCCA
  CTCCGGGCAGGATTCAGGCTATCTGGGACCGCAGGGACTGCCAGGTGCACAGCCCTGGCT
  CCCGAGGCAGGCAGGCAAGGTGACGGGACTGGAAGCCCTTTTCAGAGCCTTGGAGGAGCT
  GGTCCGTCCACAAGCAATGAGTGCCACTCTGCAGTTTGCAGGGGATGGATAAACAGGGAA
  ACACTGTGCATTCCTCACAGCCAACAGTGTAGGTCTTGGTGAAGCCCCGGCGCTGAGCTA
  AGCTCAGGCTGTTCCAGGGAGCCACGAAACTGCAGGTAGTGATGTGCAAGAGTCCATCCT
  GCAGTTTTCCAGCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGC
  AGACACTCTCCATGGCGGGGGTGTAGACCAACCGGATGTCANCGGCATCCCCTAAGGCTT
  GGAACCCTTTATACATCTTGGCCATCTTGATCTCATAACGCTGGTATAAGGTGGTCTGGT
  TGACTTCTGGTGTCCCCACAAACTTGGCCCTGATGACCAGGTCCGAACTGCCACAACGCC
  GCCTGTGGGTGGCGCGGGACACAGGTGCAAGCCCTGCTGGGGGCTATCAACCCCCGCAAC
  CCAAGATGCCAAACCCAGGGGCTCAAAGGGGCTCTGGGGGGTTCCCCTGTGCTTTCTTGG
  ACGCTGGCTCCGGCCAACCCCGGGCCCGCCACANCCAGCCGATTCAAAATCCCGACGGTT
  TCTAAACAAGTTCCGTTTATATAACTTCCCAGTACCCCCCCCCCCCATCGCCCCACGGGC
  GCGGTCTTCGAATTCCGACAACACTGTTCCCTTGGCCCCACAAACCCCCTGGTCTTCAGG
  GTCGCCCCTCTCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003254
• Insert Size: 870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003254.2, NP_003245.1
• RefSeq Size: 931 bp
• RefSeq ORF: 624 bp
• Locus ID: 7076
• Cytogenetics: Xp11.3
• Domains: NTR
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]'