TIMP2 (NM_003255) Human Untagged Clone

CAT#: SC118083

TIMP2 (untagged)-Human TIMP metallopeptidase inhibitor 2 (TIMP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TIMP2
• Synonyms: CSC-21K; DDC8
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003255, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCGCCGCGGCCCGCACCCTGCGGCTGGCGCTCGGCCTCCTGCTGCTGGCGACGCTGCTTCGCCCGG
  CCGACGCCTGCAGCTGCTCCCCGGTGCACCCGCAACAGGCGTTTTGCAATGCAGATGTAGTGATCAGGGC
  CAAAGCGGTCAGTGAGAAGGAAGTGGACTCTGGAAACGACATTTATGGCAACCCTATCAAGAGGATCCAG
  TATGAGATCAAGCAGATAAAGATGTTCAAAGGGCCTGAGAAGGATATAGAGTTTATCTACACGGCCCCCT
  CCTCGGCAGTGTGTGGGGTCTCGCTGGACGTTGGAGGAAAGAAGGAATATCTCATTGCAGGAAAGGCCGA
  GGGGGACGGCAAGATGCACATCACCCTCTGTGACTTCATCGTGCCCTGGGACACCCTGAGCACCACCCAG
  AAGAAGAGCCTGAACCACAGGTACCAGATGGGCTGCGAGTGCAAGATCACGCGCTGCCCCATGATCCCGT
  GCTACATCTCCTCCCCGGACGAGTGCCTCTGGATGGACTGGGTCACAGAGAAGAACATCAACGGGCACCA
  GGCCAAGTTCTTCGCCTGCATCAAGAGAAGTGACGGCTCCTGTGCGTGGTACCGCGGCGCGGCGCCCCCC
  AAGCAGGAGTTTCTCGACATCGAGGACCCATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003255 unedited
  ATAGGGCGGGCCGCGATTCGGCACGAGGGGTAGGAGAGTAAGTTTGCGCGGCCGAGCGGG
  GCAGGTGAGGAGGGTGAGCCGCGCGGGAGGGGCCCGCCTCGGCCCCGGCTCAGCCCCCGC
  CCGCGCCCCCAGCCCGCCGCCGCGAGCAGCGCCCGGACCCCCCACGGCGGCCCCCGCCCG
  CCCAGCCCCCCGGCCCGCCATGGGCGCCGCGGCCCGCACCCTGCGGCTGGCGCTCGGCCT
  CCTGCTGCTGGCGACGCTGCTTCGCCCGGCCGACGCCTGCAGCTGCTCCCCGGTGCACCC
  GCAACAGGCGTTTTGCAATGCAGATGTAGTGATCAGGGCCAAAGCGGTCAGTGAGAAGGA
  AGTGGACTCTGGAAACGACATTTATGGCAACCCTATCAAGAGGATCCAGTATGAGATCAA
  GCAGATAAAGATGTTCAAAGGGCCTGAGAAGGATATAGAGTTTATCTACACGGCCCCCTC
  CTCGGCAGTGTGTGGGGTCTCACTGGACGTTGGAGGAAAGAAGGAATATCTCATTGCAGG
  AAAGGCCGAGGGGGACGGCAAGATGCACATCACCCTCTGTGACTTCATCGTGCCCTGGGA
  CACCCTGAGCACCACCCAGAAGAAGAGCCTGAACCACAGGTACCAGATGGGCTGCGAGTG
  CAAGATCACGCGCTGCCCCATGATCCCGTGCTACATCTCCTCCCCGGACGAGTGCCTCNT
  GGATGGACTGGGGTCACAGAGAGAACATTAACGGGCACCAAGCCAAGTTTCTCGCCTGCA
  TAAAGAGAAGTGACGGGCTCTGTGCGTGGTACCGGGGGGCGCGGCCCCCAAGCAGGAGGT
  TCTGGACATCGAGACCCATAAGCAGGCCTCCCACGCCCCTGGGGGCAACTGCAAAAAACC
  CTCCAGGGTTTCGACTGGNCAGCTCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003255 unedited
  AATTCTCATGNCGCGCGGCCCGCAGTTCTAGCGATCNGCCTTTTTTTTTTTTTTTTTTTC
  TCTTTGAAATACTGTTTATTGTCCACGGACCGAGCGATTGCTCAAGAATGATACAAAGCA
  TCAGAGACATGCGCAGTCTGCTTGACAACTTTCAACAACTCTTGTGTGTTCCCAGCACCG
  GAAGGTCTCAAACTTCATATTCCAGCATAAACACAGTGCTCCCCTCCCCCAAAAATCCAA
  ACGGAAACAAAATCAACAGAGCTTTAAATTACGGCAGCAAGTCCAATATTTCTTAATACT
  TATCAAGCAAAGATTTGAAAATAACTACAATGTAAACTTTATTTTAAATATTTTGAGTTG
  CTTGCAGGATGAAAATAGGAGAAGAGAGCTGTGCTTAGAACATAACATATAAATTAAACG
  ATGCCAAATGGAGAGCCTTCTTAAACTACATATATATTGCCACTATTTATTGTGAAGGTA
  TTTGAGCGGCTTCCTCTGTCAGCTGCCACCCGGCTCTTCTTAACCTGTTTTGTTTTCTGC
  TCACCACGGTTAAAAGACCAACGTGTGTGGATCAAATATAAAGGCCACACCTTTCAGACC
  GAACCTACTCAAAGATCCTTTACTTTGCAATAATTTGAACTGGAGAACCAAAGACGGGAG
  ACGAATGCAAGCAAAGATGCTCAAAGAACCAAAGGAAAGCACCTGAGGAATCCACCTGCA
  TAGGCCACGCGTTCCACTCCTGGTCAAATGCTTTCACGATGCAGAAACCCTTTTTTAAAA
  AGGGCAAGCCTAATACCTACCAGGGTATTAAAAAACCCGCCCAGGGATGGATACCGCTGA
  TGTCAAAACCAAACCAAAACTTTAAAAACCATCCGCCGAAAAAGAGTAAATGGTCCTATT
  GTCTCCCCTACAGGACCGAACAGGTGGGGTATGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003255
• Insert Size: 3630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003255.4, NP_003246.1
• RefSeq Size: 3670 bp
• RefSeq ORF: 663 bp
• Locus ID: 7077
• Cytogenetics: 17q25.3
• Domains: NTR
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]'