Thymidine Kinase 1 (TK1) (NM_003258) Human Untagged Clone

CAT#: SC118084

TK1 (untagged)-Human thymidine kinase 1, soluble (TK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TK1
• Synonyms: TK2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118084 sequence for NM_003258 edited (data generated by NextGen Sequencing)
  ATGAGCTGCATTAACCTGCCCACTGTGCTGCCCGGCTCCCCCAGCAAGACCCGGGGGCAG
  ATCCAGGTGATTCTCGGGCCGATGTTCTCAGGAAAAAGCACAGAGTTGATGAGACGCGTC
  CGTCGCTTCCAGATTGCTCAGTACAAGTGCCTGGTGATCAAGTATGCCAAAGACACTCGC
  TACAGCAGCAGCTTCTGCACACATGACCGGAACACCATGGAGGCGCTGCCCGCCTGCCTG
  CTCCGAGACGTGGCCCAGGAGGCCCTGGGCGTGGCTGTCATAGGCATCGACGAGGGGCAG
  TTTTTCCCTGACATCGTGGAGTTCTGCGAGGCCATGGCCAACGCCGGGAAGACCGTAATT
  GTGGCTGCACTGGATGGGACCTTCCAGAGGAAGCCATTTGGGGCCATCCTGAACCTGGTG
  CCGCTGGCCGAGAGCGTGGTGAAGCTGACGGCGGTGTGCATGGAGTGCTTCCGGGAAGCC
  GCCTATACCAAGAGGCTCGGCACAGAGAAGGAGGTCGAGGTGATTGGGGGAGCAGACAAG
  TACCACTCCGTGTGTCGGCTCTGCTACTTCAAGAAGGCCTCAGGCCAGCCTGCCGGGCCG
  GACAACAAAGAGAACTGCCCAGTGCCAGGAAAGCCAGGGGAAGCCGTGGCTGCCAGGAAG
  CTCTTTGCCCCACAGCAGATTCTGCAATGCAGCCCTGCCAACTGA
  
  Clone variation with respect to NM_003258.4
  33 t=>c;225 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003258 unedited
  CACACCACGGCCCACNACCCCCTTCCACCCGCCTTGTTCACATTTGTATACGACTCACTA
  TAGGCGGCCGCGNATATCGCACGAGCGGGAGCGCAATGAGCTGCATTAACCTGCCCATGT
  GCTGCCCGGCTCCCCCAGCAAGACCCGGGGGCAGATCCAGGTGATTCTCGGGCCGATGTT
  CTCAGGAAAAAGCACAGAGTTGATGAGACGCGTCCGTCGCTTCCAGATTGCTCAGTACAA
  GTGCCTGGTGATCAAGTATGCCAAAGACACTCGCTACAGCAGCAGCTTCTGCACACATGA
  CCGGAACACCATGGAGGCGCTGCCCGCCTGCCTGCTCCGAGACGTGGCCCAGGAGGCCCT
  GGGCGTGGCTGTCATAGGCATCGACGAGGGGCAGTTTTTCCCTGACATCGTGGAGTTCTG
  CGAGGCCATGGCCAACGCCGGGAAGACCGTAATTGTGGCTGCACTGGATGGGACCTTCCA
  GAGGAAGCCATTTGGGGCCATCCTGAACCTGGTGCCGCTGGCCGAGAGCGTGGTGAAGCT
  GACGGCGGTGTGCATGGAGTGCTTCCGGGAAGCCGCCTATACCAAGAGGCTCGGCACAGA
  GAAGGAGGTCGAGGTGATTGNGGGAGCAGACAAGTACCACTCCGTGTGTCGGCTCTGCTA
  CTTCAAGAAAGCCTCAGGCCAGCCTGCCGGGCCGGACAACANAGAAACTGCCCAGTGCCA
  GGAAAGCCAGGGGAAGCCGTGGCTGCCAGAAAGCTCTTTGCCCCACAGCAGATTCTGCAA
  TGCAGCCCTGTCCACTGAGGGACCTGCGATGGCCGNCCGCTCCCTTCCTGCCACTGGCGN
  CTACTGGACCCTGCCCTGCATGCTTGCCAGCCACTCCCAGAAGAAGTCGGGAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003258 unedited
  GGGTTGCTATGGAACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCCCCCC
  GGGAATTTTCAATTATTTAATTTCAAAACCTACAGCAAAGGGGGGGCCCCGGCCCTTCCC
  CCCTTAAAAAAAAACCCCCGGGGATCCAATTCCCAAAAGGGTGGGGCCCCCCCATTTTGG
  GAAAAAATGCTTTTTTTCCTGGGGAAACCCTCCCCAAAGGTGGGAAGGGTTTTGACCCCA
  AAAGGAAACAAAAGGGGGGGAACCCCGCCCCCCCCCGGGGGGGGGGGGGACCCCCTCCCA
  GGCAAAAGGGCCTAACCCTCATGGGGGGCCAAGGCTAAAAGGGGCTGGCCCAGGCTTCCC
  AGGGAAAAAAAAAACCCCCCTTTGAAACCCCCAAAGGGGAAAAACCAGGCTGATTTCAAC
  AGGGGGGGGCTTTTCCCCCCACCCCAAGGAGAGGGAGGGGGCCAAATCCCAGGCCACCAA
  GGGGGGCCGGTTCCACCCGGGGCAATCTTCCCCCCCCCTGAAAGGGAACCTTTAAACAAA
  CCATTGGGTAAAAAAAAAAGGAACTTGCCCCGGGGGGGGCCCCCCCACAAGAGAAATTCT
  CCAAAGGCCAAGGTGTGGGTATCTTCCCGCCTCCCAATTTTTTTTGGAAGGGCTGGGCAC
  ATGCAGGGGAACCTCCCATTAGCCGCAATTGCAAAAAGAGACGGGGCCCCCCTCGAAGGT
  CCCTTATTTGGGAGGGCTCCTTTTAAAAAATTGGGTGGGGGAAAAAAATTTCTGGGGGCC
  CCGGGTTTCCCTGGTTTTTTGGGCGGGGGGAAGTTTTTTTTTTTTTCCGCCCCGAAGGTG
  GGCTTAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003258
• ORF Size: 705 bp
• Insert Size: 1500
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_003258.1, NP_003249.1
• RefSeq Size: 1421
• RefSeq ORF: 705
• Locus ID: 7083
• Domains: TK
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism
• Gene Summary: The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [provided by RefSeq, Oct 2016]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).