TSPAN4 (NM_003271) Human Untagged Clone

CAT#: SC118091

TSPAN4 (untagged)-Human tetraspanin 4 (TSPAN4), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TSPAN4
• Synonyms: NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118091 sequence for NM_003271 edited (data generated by NextGen Sequencing)
  ATGGCGCGCGCCTGCCTCCAGGCCGTCAAGTACCTCATGTTCGCCTTCAACCTGCTCTTC
  TGGCTGGGAGGCTGTGGCGTGCTGGGTGTCGGCATCTGGCTGGCCGCCACACAGGGGAGC
  TTCGCCACGCTGTCCTCTTCCTTCCCGTCCCTGTCGGCTGCCAACCTGCTCATCATCACC
  GGCGCCTTTGTCATGGCCATCGGCTTCGTGGGCTGCCTGGGTGCCATCAAGGAGAACAAG
  TGCCTCCTGCTCACTTTCTTCCTGCTGCTGCTGCTGGTGTTCCTGCTGGAGGCCACCATC
  GCCATCCTCTTCTTCGCCTACACGGACAAGATTGACAGGTATGCCCAGCAAGACCTGAAG
  AAAGGCTTGCACCTGTACGGCACGCAGGGCAACGTGGGCCTCACCAACGCCTGGAGCATC
  ATCCAGACCGACTTCCGCTGCTGTGGCGTCTCCAACTACACTGACTGGTTCGAGGTGTAC
  AACGCCACGCGGGTACCTGACTCCTGCTGCTTGGAGTTCAGTGAGAGCTGTGGGCTGCAC
  GCCCCCGGCACCTGGTGGAAGGCGCCGTGCTACGAGACGGTGAAGGTGTGGCTTCAGGAG
  AACCTGCTGGCTGTGGGCATCTTTGGGCTGTGCACGGCGCTGGTGCAGATCCTGGGCCTG
  ACCTTCGCCATGACCATGTACTGCCAAGTGGTCAAGGCAGACACCTACTGCGCGTAG
  
  Clone variation with respect to NM_003271.4
  166 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003271 unedited
  ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGCGCGAGCGGGAGGCGG
  CGGCGCAGAGCTTGGGGCTTCCTTGGTCGCACCCACCACCTGCCTGCCCACTGGTCAGCC
  TTCAGGGACCCTGAGCACCGCCTGGTCTCTTTCCTGTGGCCAGCCCAGAACTGAAGCGCT
  GCGGCATGGCGCGCGCCTGCCTCCAGGCCGTCAAGTACCTCATGTTCGCCTTCAACCTGC
  TCTTCTGGCTGGGAGGCTGTGGCGTGCTGGGTGTCGGCATCTGGCTGGCCGCCACACAGG
  GGAGCTTCGCCACGCTGTCCTCTTCCTTCCCGTCCCTGTCGGCTGCCAACCTGCTCATCA
  TCACCGGCGCCTTTGTCATGGCCATCGGCTTCGTGGGCTGCCTGGGTGCCATCAAGGAGA
  ACAAGTGCCTCCTGCTCACTTTCTTCCTGCTGCTGCTGCTGGTGTTCCTGCTGGAGGCCA
  CCATCGCCATCCTCTTCTTCGCCTACACGGACAAGATTGACAGGTATGCCCAGCAAGACC
  TGAAGAAAGGCTTGCACCTGTACGGCACGCAGGGCAACGTGGGCCTCACCAACGCCTGGA
  GCATCATCCAGACCGACTTCCGCTGCTGTGGCGTCTCCAACTACACTGACTGGTTCGAGG
  TGTACAACGCCACGCGGGTACCTGACTCCTGCTGCTTGGAGNTCAATGAGAGCTGTGGGC
  TGCACGCCCCCGGCACCTGGTGGAANGGCGCCGTGCTACNAGACNGTGAANGGTGTGGCT
  NCAAGAGAACCTGCTGGCTGTNGGCATCTTTGGGCTGTGCACGGCGCTGGTGCAAATCCT
  GGGCCTGACCCTCNCATGACCATGTACTGGNCAGTGGTCAAAGGCAGAACCTACTGCGCG
  TAGGCCGCCCACGNCCGCTTCTCTGCAAAGGACGCCACGGGGAGATGGGCGACCACANTG
  GCTTNNCACCACAGNCTNGGGCTTGCCCATGCTGAGAGGNAGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003271 unedited
  CCGCATTCTAGNATCGAGTTTTTTTTTTTTTTTTTTGCTGCTCACACTTTATTAAGATGC
  ACCAGGAGCCCCATGGGCCCACTATGGAATGTAGGTGAGGGGTCCACGGCCCCGCCTGGA
  GCACCAGGACCAGTGGGGCCACCTCCAGGATGCCAAGACCCGGCTCCTCCAGCGCCAACC
  TGTTTTCCAGGAGGCTGGGGGCCACAGGCTGGCTCCCGTGTGAACACTGCTTTGGAGAAT
  ACGTAAATATAAAAAGTGCTGGAGGCCCCTCCCACCCCTGCCCTGGGTTCCGCAGCCAGC
  ACGTGGCCACCCCTCCCAGGGGGGGTCCGGAGGCCCTGAAGCCACCTGAGCCAGGGCCTT
  CCAGAAACAGGGTTCCGGGGGCTCCAGGCACCTGTCCCTCCCTCCCTCCTCCCAGCATGG
  GGCATAGCACCGAGGCTGGTGGTGGGAAAGGCAGCTGTGGGTGCGGCCATCTCCCCGTGG
  GCGTCCTTTTGGCAGATAATCGGGCGGTGGGCGGCCTACGCGCAGTAAGTGTCTGCCTTG
  ACCACTTGGCAGTACATGGTCATGGCGAAAGTCATGCCCNAGATCTGCACCAGCGCCGTG
  CACAGCCCATAGATGCCCACAGCCAGCATGTTCTCCTGAAGCCACACCTTCACCGTCTCG
  TAGCACGGCGCCTTCCACCANGTGCCGGAGGCGTGCANGCCACAGCTCTCACTGAACTNC
  AAGCANCAGGAGTCAAGTACCCGCGTGGCGTTGTACACCTCGAACCATTCAGTGTAGTTG
  GAGACGCCCACCANCGAAGTCGGCCTGGTGATGCTCCAGCCGTTGGGATGCCCACGTGCC
  CTGCGTGCCGAAAGTGCGAGCCCTTCTTAGGTCTGCTGGACTACCTGCATCTTGCCCGGT
  AGCGAAAATAGATGCCATGGGGGCCTCCCGGACCCCNCCCCCACCAGATTAGTGACCGAG
  GCCTTGTCTCTGATGCCCAAGCACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003271
• Insert Size: 1360 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003271.4, NP_003262.1
• RefSeq Size: 1429 bp
• RefSeq ORF: 717 bp
• Locus ID: 7106
• Cytogenetics: 11p15.5
• Domains: transmembrane4
• Protein Families: Transmembrane
• Gene Summary: 'The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein and is similar in sequence to its family member CD53 antigen. It is known to complex with integrins and other transmembrane 4 superfamily proteins. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (5) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, 4, 5 and 6 encode the same isoform (a).