Topoisomerase I (TOP1) (NM_003286) Human Untagged Clone

CAT#: SC118105

TOP1 (untagged)-Human topoisomerase (DNA) I (TOP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TOP1
• Synonyms: TOPI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003286 edited
  ATGAGTGGGGACCACCTCCACAACGATTCCCAGATCGAAGCGGATTTCCGATTGAATGAT
  TCTCATAAACACAAAGATAAACACAAAGATCGAGAACACCGGCACAAAGAACACAAGAAG
  GAGAAGGACCGGGAAAAGTCCAAGCATAGCAACAGTGAACATAAAGATTCTGAAAAGAAA
  CACAAAGAGAAGGAGAAGACCAAACACAAAGATGGAAGCTCAGAAAAGCATAAAGACAAA
  CATAAAGACAGAGACAAGGAAAAACGAAAAGAGGAAAAGGTTCGAGCCTCTGGGGATGCA
  AAAATAAAGAAGGAGAAGGAAAATGGCTTCTCTAGTCCACCACAAATTAAAGATGAACCT
  GAAGATGATGGCTATTTTGTTCCTCCTAAAGAGGATATAAAGCCATTAAAGAGACCTCGA
  GATGAGGATGATGCTGATTATAAACCTAAGAAAATTAAAACAGAAGATACCAAGAAGGAG
  AAGAAAAGAAAACTAGAAGAAGAAGAGGATGGTAAATTGAAAAAACCCAAGAATAAAGAT
  AAAGATAAAAAAGTTCCTGAGCCAGATAACAAGAAAAAGAAGCCGAAGAAAGAAGAGGAA
  CAGAAGTGGAAATGGTGGGAAGAAGAGCGCTATCCTGAAGGCATCAAGTGGAAATTCCTA
  GAACATAAAGGTCCAGTATTTGCCCCACCATATGAGCCTCTTCCAGAGAATGTCAAGTTT
  TATTATGATGGTAAAGTCATGAAGCTGAGCCCCAAAGCAGAGGAAGTAGCTAC:GTTC:T
  TTGCAAAAATGCTCGACCATGAATATACTACCAAGGAAATATTTAGGAAAAATTTCTTTA
  AAGACTGGAGAAAGGAAATGACTAATGAAGAGAAGAATATTATCACCAACCTAAGCAAAT
  GTGATTTTACCCAGATGAGCCAGTATTTCAAAGCCCAGACGGAAGCTCGGAAACAGATGA
  GCAAGGAAGAGAAACTGAAAATCAAAGAGGAGAATGAAAAATTACTGAAAGAATATGGAT
  TCTGTATTATGGATAACCACAAAGAGAGGATTGCTAACTTCAAGATAGAGCCTCCTGGAC
  TTTTCCGTGGCCGCGGCAACCACCCCAAGATGGGCATGCTGAAGAGACGAATCATGCCCG
  AGGATATAATCATCAACTGTAGCAAAGATGCCAAGGTTCCTTCTCC:TCCTCCAGGACAT
  AAGTGGAAAGAAGTCCGGCATGATAACAAGGTTACTTGGCTGGTTTCCTGGACAGAGAAC
  ATCCAAGGTTCCATTAAATACATCATGCTTAACCCTAGTTCACGAATCAAGGGTGAGAAG
  GACTGGCAGAAATACGAGACTGCTCGGCGGCTGAAAAAATGTGTGGACAAGATCCGGAAC
  CAGTATCGAGAAGACTGGAAGTCCAAAGAGATGAAAGTCCGGCAGAGAGCTGTAGCCCTG
  TACTTCATCGACAAGCTTGCTCTGAGAGCAGGCAATGAAAAGGAGGAAGGAGAAACAGCG
  GACACTGTGGGCTGCTGCTCACTTCGTGTGGAGCACATCAATCTACACCCAGAGTTGGAT
  GGTCAGGAATATGTGGTAGAGTTTGACTTCCTCGGGAAGGACTCCATCAGATACTATAAC
  AAGGTCCCTGTTGAGAAACGAGTTTTTAAGAACCTACAACTATTTATGGAGAACAAGCAG
  CCCGAGGATGATCTTTTTGATAGACTCAATACTGGTATTCTGAATAAGCATCTTCAGGAT
  CTCATGGAGGGCTTGACAGCCAAGGTATTCCGTACATACAATGCCTCCATCACGCTACAG
  CAGCAGCTAAAAGAACTGACAGCCCCGGATGAGAACATCCCAGCGAAGATCCTTTCTTAT
  AACCGTGCCAATCGAGCTGTTGCAATTCTTTGTAACCATCAGAGGGCACCACCAAAAACT
  TTTGAGAAGTCTATGATGAACTTGCAAACTAAGATTGATGCCAAGAAGGAACAGCTAGCA
  GATGCCCGGAGAGACCTGAAAAGTGCTAAGGCTGATGCCAAGGTCATGAAGGATGCAAAG
  ACGAAGAAGGTAGTAGAGTCAAAGAAGAAGGCTGTTCAGAGACTGGAGGAACAGTTGATG
  AAGCTGGAAGTTCAAGCCACAGACCGAGAGGAAAATAAACAGATTGCCCTGGGAACCTCC
  AAACTCAATTATCTGGACCCTAGGATCACAGTGGCTTGGTGCAAGAAGTGGGGTGTCCCA
  ATTGAGAAGATTTACAACAAAACCCAGCGGGAGAAGTTTGCCTGGGCCATTGACATGGCT
  GATGAAGACTATGAGTTTTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003286 unedited
  TTTTGTAATACGACTCCACTATAGGGCCGCGCCGCGCAATTCGGCACGAGGGTTACACAA
  CTGCTGGGGTCTGTTCTCGCCGCCCGCCCGGCAGTCAGGCAGCGTCGCCGCCGTGGTAGC
  AGCCTCAGCCGTTTCTGGAGTCTCGGGCCCACAGTCACCGCCGCTTACCTGCGCCTCCTC
  GAGCCTCCGGAGTCCCCGTCCGCCCGCACAGGCCGGTTCGCCGTCTGCGTCTCCCCCACG
  CCGCCTCGCCTGCCGCCGCGCTCGTCCCTCCGGGCCGACATGAGTGGGGACCACCTCCAC
  AACGATTCCCAGATCGAAGCGGATTTCCGATTGAATGATTCTCATAAACACAAAGATAAA
  CACAAAGATCGAGAACACCGGCACAAAGAACACAAGAAGGAGAAGGACCGGGAAAAGTCC
  AAGCATAGCAACAGTGAACATAAAGATTCTGAAAAGAAACACAAAGAGAAGGAGAAGACC
  AAACACAAAGATGGAAGCTCAGAAAAGCATAAAGACAAACATAAAGACAGAGACAAGGAA
  AAACGAAAAGAGGAAAAGGTTCGAGCCTCTGGGGATGCAAAATAAAGAAGGAGAAGGAAA
  ATGGCTTCTCTAGTCCACCACATATTAAAGATGAACCTGAAGATGATGGCTATTTTGTTC
  CTCCTAAAGAGGATTATAAGCCATTAAAGAGACCTCGAGATGAGGATGATGCTGATTATT
  ACCCTAGGAAATTAAACCAGAGGATCCCAGAAAGAGATGAAAGAAACTTGAAGAGAAGAG
  GGTGGTAAATTGAAAAACCCAAAATTAAGTACAAGAAAAAAAGTTCCTGGGCCCTCATAC
  ATGAACAGCACCCCTAGCAAGAAGAGAACCTAACGGAAATGGTGGAAGAACAGCCCTTCC
  TGAAGCCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003286 unedited
  CTATGCTACCGCGTGCCGCATNCTAAAATCGAGTTTTTTTTTTTTTTTTTTCCTTAAGCT
  GTAGGAATTTATTTTAAATAACCTACAGTTGATTAAAAGGGAATTCATGATAAAAATACA
  AGATACTTGAGGAAAGATGTGGGAAATGGACTCTGCACACACACTAAACTAACAATGCCT
  CTAAAACTAATGATTATAGCAAAAAATGTCTTCACATTAAAATTCTGCTTTTTATGTTTT
  TTTCCATTTTTTACACAATTACAAAAGAAAAAATAAAAGCCCTAAAATCTTGATTATTTT
  TCCTTTTTTTGGACCAAATACTCATTTTCCTCTAAGTTTATTGACCTGTGAAACTTTTTA
  TACAATAAAATCTTTCAAGTGAAAGATTAGGGTTAAAAAGAAAAAGATGGATATCTTAAA
  GGGTACAGCGAATGCTCAGAACAAAGGATGATGGGCAAAATGGTTTCAGCCACTGATTAT
  TCCATTATCCTTAGATTCACTCGCCCTTGATCCCTCCCCAACCCCAATTTACACGATCTT
  TAAGATCAAGAAAAAGGTTTAAATATTTTAAAATAATCAAAAAGAAATAAAAATTCACAT
  TTAAAAAGGAACACTGAAGCCAGGAAACATTTCGCCATCATGCTTTCCCTGTGAACAGGT
  GTTTGACCCAAAACACTGCCAAAGGCACGACCGCTTCAAGCTTAATGAAAATTGATCCCC
  CCGACAATAGAGAGTGACGACTCTAACAGGCGCGCCGGGATTTTTGCCCTACTTAACCTT
  AACACCTGAAAGGGAGAGAGCAAGGGAGCCTAGAATTTATTCCTATCCAATATAGAGCTA
  TTGGGAACCCTACTGTAAAAAGCACGATATAACCGACCTTCATCACGCCCTAAGAGACCC
  AACTTTCACGAAACGCGAACTGCCTGGAAAACCTGAAACATCCAATCCTGCCTCTGGAAC
  AAAACAGATTCAACACAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003286
• ORF Size: 2298 bp
• Insert Size: 3700
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_003286.2, NP_003277.1
• RefSeq Size: 3734
• RefSeq ORF: 2298
• Locus ID: 7150
• Domains: TOPEUc
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]