Synaptotagmin V (SYT5) (NM_003180) Human Untagged Clone

CAT#: SC118116

SYT5 (untagged)-Human synaptotagmin V (SYT5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SYT5
• Synonyms: synaptotagmin 5; synaptotagmin V
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118116 sequence for NM_003180 edited (data generated by NextGen Sequencing)
  ATGTTCCCGGAGCCCCCAACCCCGGGGCCTCCATCGCCCGACACGCCTCCCGACTCCAGT
  CGCATCAGCCACGGCCCAGTGCCCCCCTGGGCCCTGGCCACCATCGTGCTGGTCTCAGGC
  CTCCTCATCTTCAGCTGCTGTTTCTGTCTCTACCGGAAGAGCTGTCGGAGGCGGACAGGC
  AAGAAGAGCCAGGCCCAAGCCCAGGTCCACCTTCAGGAAGTGAAGGGGCTGGGCCAGAGT
  TACATAGACAAGGTGCAGCCAGAAGTAGAGGAGCTGGAGCCAGCACCATCCGGGCCAGGG
  CAGCAGGTGGCAGACAAGCATGAGCTAGGACGACTGCAGTACTCCCTGGATTATGACTTC
  CAGAGTGGCCAGCTGCTGGTGGGCATTCTGCAAGCAATGGGATTGGCAGCCTTGGATCTT
  GGTGGCTCCTCGGACCCCTATGTGCGGGTCTACCTGCTGCCGGACAAACGGAGGCGGTAC
  GAGACCAAGGTGCATCGGCAGACGCTGAACCCTCACTTTGGGGAGACCTTCGCCTTCAAG
  GTCCCCTACGTGGAGCTGGGGGGCAGGGTGCTGGTCATGGCGGTGTACGACTTCGACCGC
  TTCTCTCGCAATGACGCCATCGGGGAGGTGCGGGTCCCTATGAGCTCCGTGGACCTGGGG
  CGGCCAGTGCAGGCCTGGCGGGAGCTGCAGGCGGCTCCGCGGGAGGAGCAGGAGAAGCTT
  GGGGACATCTGCTTCTCCCTCCGCTATGTCCCCACGGCCGGGAAGCTCACCGTCATCGTC
  CTGGAGGCTAAAAACCTGAAGAAGATGGACGTAGGAGGACTGTCAGATCCATACGTCAAG
  GTCCACCTGCTGCAGGGCGGCAAAAAGGTGCGGAAGAAGAAAACCACCATCAAGAAGAAC
  ACTCTGAACCCCTATTACAACGAAGCTTTCAGCTTCGAGGTGCCCTGTGACCAAGTCCAG
  AAGGTGCAGGTGGAGCTGACCGTGCTGGACTACGACAAGCTGGGCAAGAACGAGGCCATC
  GGGAGGGTGGCCGTGGGGGCGGCCGCCGGCGGGGCTGGCCTGCGGCACTGGGCGGACATG
  CTGGCCAACCCGCGGCGGCCCATTGCCCAGTGGCACTCGCTGCGGCCCCCGGACCGAGTG
  AGGCTGCTGCCTGCGCCCTGA
  
  Clone variation with respect to NM_003180.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003180 unedited
  GTATACGACTCACTATAGGCGGCACGCGAATTCGCACGAGGGCCGCCGAGCAGGGTGCTT
  GAGTGTCTCTGCAGAAAAGACTCCAGGACCCCGCCACCATGTTCCCGGAGCCCCCAACCC
  CGGGGCCTCCATCGCCCGACACGCCTCCCGACTCCAGTCGCATCAGCCACGGCCCAGTGC
  CCCCCTGGGCCCTGGCCACCATCGTGCTGGTCTCAGGCCTCCTCATCTTCAGCTGCTGTT
  TCTGTCTCTACCGGAAGAGCTGTCGGAGGCGGACAGGCAAGAAGAGCCAGGCCCAAGCCC
  AGGTCCACCTTCAGGAAGTGAAGGGGCTGGGCCAGAGTTACATAGACAAGGTGCAGCCAG
  AAGTAGAGGAGCTGGAGCCAGCACCATCCGGGCCAGGGCAGCAGGTGGCAGACAAGCATG
  AGCTAGGACGACTGCAGTACTCCCTGGATTATGACTTCCAGAGTGGCCAGCTGCTGGTGG
  GCATTCTGCAAGCAATGGGATTGGCAGCCTTGGATCTTGGTGGCTCCTCGGACCCCTATG
  TGCGGGTCTACCTGCTGCCGGACAAACGGAGGCGGTACGAGACCAAGGTGCATCGGCAGA
  CGCTGAACCCTCACTTTGGGGAGACCTTCGCCTTCAAGGTCCCCTACGTGGAGCTGGGGG
  GCAGGGTGCTGGTCATGGCGGTGTACGACTTCGACCGCTTCTCTCGCAATGACGCCATCG
  GAGAAGTGCGGGTCCCTATGAGCTNCGTGGACCTGNGGCGGNCAGTGCANGCCTGGCAGG
  AGCTGCAAGCGGCTCCGCAGGAAGAGGAGAAGCTNGNGGACATCTGCTTCTNCCCTCCGC
  TATGTCCCACGGNCGGGGAGCTCACCGTCATCGTCCTGGAAGCTAAAAACCTGAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003180 unedited
  ATGGACCGGCGGCCGCAATCTAGAGTCGAGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTAACCGGAGCTGCTGAATATTAATTGGGCCCCAAAAGCAGGGCAGGGATGGGCTGTGT
  TGGAAAAAAGAGGAAAGCAAACGAGCTATGGCTGATTGTCAAAGCAGGGGGCAGCACCCG
  GGGGCAGGAAAAACCAGGCTGCCTTCCCTTCCCGGGGTCCCTGGGGCATCTGGGTGTGTC
  CGCGAGGGTCGGGGGAGTGTGCTGGAAAGTTGCCGTGATTGGCATCGTTGGGGGTGAAAA
  AACATGGAATGGTTCAAATGGTTGGGGTGGAAGGGGGGGGGAGGGTAACCGCCAAAGGAA
  GCTTAAGGGGGGGGCTGGCTTGGTTTTGGCCGGTCTCGGGAGTCTGGGGTCAGGGGCTAA
  AGTCCAGGCTTGCCCGGGGGCTCGGGGCGGGAGTAAGGGCCCAGGCACAACCCTAACTCG
  GTCCGGGGGCCGCAGCGAGTGCCACTGGGCACTGGGCCGCCGCCCTTTGCCAATTATGTC
  CGTCAGCGGCCGAACTCAACCCGCCGGGGGGCGCCCCAAGCCCCCCTCCCGAGGACTTGT
  CCTGCCCACCTTGCTAATCCCACCCGTCACCCCCCACTGACTTCTGTCCCCGCCCCAGGC
  TCCTCCTACCCCAACCTTCCCCCCACTGGTCTACCCCACCCACTTCCGTTCGTCCCCTCC
  TCCCCCCCCTCCCCGCCATCCTCTGCCATCTTTTTCCCTCCTTTTCCCCCCCCCTCCCCC
  CTTATCCCACATTCTCTCTATTTCGCCCCTTCCCTTCTCCCCCCTCCCCCGCCTTTCCTC
  TTTCCCCCTCCCCCCTTTCCCTACTCTCCCCCTCTTCACCCCCTCTTCGCCACCCCTATC
  TCCCGCCCTTCCTACTCCCCCTCCTCCCCATCCCACCCCAATTCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003180
• Insert Size: 1840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003180.1, NP_003171.1
• RefSeq Size: 2052 bp
• RefSeq ORF: 1161 bp
• Locus ID: 6861
• Cytogenetics: 11p
• Domains: C2
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: 'Synaptotagmins, such as SYT5, are a family of type III membrane proteins characterized by cytoplasmic repeats related to protein kinase C (see MIM 176960) regulatory (C2) domains, which are thought to bind calcium. Synaptotagmins may act both as negative regulators of vesicle fusion, allowing fusion in the presence of calcium, and as calcium receptors or sensor molecules (summary by Hudson and Birnbaum, 1995 [PubMed 7597049]).[supplied by OMIM, Feb 2011]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).