TCF4 (NM_003199) Human Untagged Clone

CAT#: SC118127

TCF4 (untagged)-Human transcription factor 4 (TCF4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCF4
• Synonyms: bHLHb19; E2-2; FECD3; ITF-2; ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; TCF-4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118127 sequence for NM_003199 edited (data generated by NextGen Sequencing)
  ATGCATCACCAACAGCGAATGGCTGCCTTAGGGACGGACAAAGAGCTGAGTGATTTACTG
  GATTTCAGTGCGATGTTTTCACCTCCTGTGAGCAGTGGGAAAAATGGACCAACTTCTTTG
  GCAAGTGGACATTTTACTGGCTCAAATGTAGAAGACAGAAGTAGCTCAGGGTCCTGGGGG
  AATGGAGGACATCCAAGCCCGTCCAGGAACTATGGAGATGGGACTCCCTATGACCACATG
  ACCAGCAGGGACCTTGGGTCACATGACAATCTCTCTCCACCTTTTGTCAATTCCAGAATA
  CAAAGTAAAACAGAAAGGGGCTCATACTCATCTTATGGGAGAGAATCAAACTTACAGGGT
  TGCCACCAGCAGAGTCTCCTTGGAGGTGACATGGATATGGGCAACCCAGGAACCCTTTCG
  CCCACCAAACCTGGTTCCCAGTACTATCAGTATTCTAGCAATAATCCCCGAAGGAGGCCT
  CTTCACAGTAGTGCCATGGAGGTACAGACAAAGAAAGTTCGAAAAGTTCCTCCAGGTTTG
  CCATCTTCAGTCTATGCTCCATCAGCAAGCACTGCCGACTACAATAGGGACTCGCCAGGC
  TATCCTTCCTCCAAACCAGCAACCAGCACTTTCCCTAGCTCCTTCTTCATGCAAGATGGC
  CATCACAGCAGTGACCCTTGGAGCTCCTCCAGTGGGATGAATCAGCCTGGCTATGCAGGA
  ATGTTGGGCAACTCTTCTCATATTCCACAGTCCAGCAGCTACTGTAGCCTGCATCCACAT
  GAACGTTTGAGCTATCCATCACACTCCTCAGCAGACATCAATTCCAGTCTTCCTCCGATG
  TCCACTTTCCATCGTAGTGGTACAAACCATTACAGCACCTCTTCCTGTACGCCTCCTGCC
  AACGGGACAGACAGTATAATGGCAAATAGAGGAAGCGGGGCAGCCGGCAGCTCCCAGACT
  GGAGATGCTCTGGGGAAAGCACTTGCTTCGATCTATTCTCCAGATCACACTAACAACAGC
  TTTTCATCAAACCCTTCAACTCCTGTTGGCTCTCCTCCATCTCTCTCAGCAGGCACAGCT
  GTTTGGTCTAGAAATGGAGGACAGGCCTCATCGTCTCCTAATTATGAAGGACCCTTACAC
  TCTTTGCAAAGCCGAATTGAAGATCGTTTAGAAAGACTGGATGATGCTATTCATGTTCTC
  CGGAACCATGCAGTGGGCCCATCCACAGCTATGCCTGGTGGTCATGGGGACATGCATGGA
  ATCATTGGACCTTCTCATAATGGAGCCATGGGTGGTCTGGGCTCAGGGTATGGAACCGGC
  CTTCTTTCAGCCAACAGACATTCACTCATGGTGGGGACCCATCGTGAAGATGGCGTGGCC
  CTGAGAGGCAGCCATTCTCTTCTGCCAAACCAGGTTCCGGTTCCACAGCTTCCTGTCCAG
  TCTGCGACTTCCCCTGACCTGAACCCACCCCAGGACCCTTACAGAGGCATGCCACCAGGA
  CTACAGGGGCAGAGTGTCTCCTCTGGCAGCTCTGAGATCAAATCCGATGACGAGGGTGAT
  GAGAACCTGCAAGACACGAAATCTTCGGAGGACAAGAAATTAGATGACGACAAGAAGGAT
  ATCAAATCAATTACTAGCAATAATGACGATGAGGACCTGACACCAGAGCAGAAGGCAGAG
  CGTGAGAAGGAGCGGAGGATGGCCAACAATGCCCGAGAGCGTCTGCGGGTCCGTGACATC
  AACGAGGCTTTCAAAGAGCTCGGCCGCATGGTGCAGCTCCACCTCAAGAGTGACAAGCCC
  CAGACCAAGCTCCTGATCCTCCACCAGGCGGTGGCCGTCATCCTCAGTCTGGAGCAGCAA
  GTCCGAGAAAGGAATCTGAATCCGAAAGCTGCGTGTCTGAAAAGAAGGGAGGAAGAGAAG
  GTGTCCTCGGAGCCTCCCCCTCTCTCCTTGGCCGGCCCACACCCTGGAATGGGAGACGCA
  TCGAATCACATGGGACAGATGTAA
  
  Clone variation with respect to NM_003199.2
  1929 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003199 unedited
  GTTTTTGTATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGGGCGCGGGAGCG
  GGCGCAGGAGCAGGCGGCGGCGGTGGCGGCGGCGGTTAGACATGAACGCCGCCTCGGCGC
  CGGCGGTGCACGGAGAGCCCCTTCTCGCGCGCGGGCGGTTTGTGTGATTTTGCTAAAATG
  CATCACCAACAGCGAATGGCTGTCTTAGGGACGGACAAAGAGCTGAGTGATTTACTGGAT
  TTCAGTGCGATGTTTTCACCTCCTGTGAGCAGTGGGAAAAATGGACCAACTTCTTTGGCA
  AGTGGACATTTTACTGGCTCAAATGTAGAAGACAGAAGTAGCTCAGGGTCCTGGGGGAAT
  GGAGGACATCCAAGCCCGTCCAGGAACTATGGAGATGGGACTCCCTATGACCACATGACC
  AGCAGGGACCTTGGGTCACATGACAATCTCTCTCCACCTTTTGTCAATTCCAGAATACAA
  AGTAAAACAGAAAGGGGCTCATACTCATCTTATGGGAGAGAATCAAACTTACAGGGTTGC
  CACCAGCAGAGTCTCCTTGGAGGTGACATGGATATGGGCAACCCAGGAACCCTTTCGCCC
  ACCAAACCTGGTTCCCAGTACTATCAGTATTCTAGCAATAATCCCCGAAGGAGGCCTCTT
  CACAGTAGTGCCATGGAGGTACAGACAAAGAAAGTTCGAAAAGTTCCTCCAGGTTTGCCA
  TCTTCAGTCTATGCTCCATCAGCAAGCACTGCCGACTACAATAGGGACTCGCCAGGCTAT
  TCTTCCTCCAAACAGCAACCAGCACTTTCCCTAGCTCTTTTTTATGCAAGATGGCATCAC
  AGCAGTGACCCTTGGAGCTCCTTCATGGGATGAATCCCCCTGCCTTTCAGGGATGTTGGG
  CAACTCTTTTCATATTCACAGTCCACACACTCTTGACTCTGATTCCACTGAACCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003199 unedited
  CGGCACGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGTTTTTCTGTTTTTTGATAA
  TTGGGAATGCTGAAACCTCTTGCGTCTGCGATTCATAACTACTCAGACTTGTCTTATATT
  ACAAAAATGGGGGTTAAGGAGAAGTGTTTATGTGGGTTTAAGATAATACAGCTGTTAAGG
  AAGTGGTCTCTTGTTTTAATGAAGCAATGTGGCAACTTGGACCCTTTTACATCTGTCCCA
  TGTGATTCGATGCGTCTCCCATTCCAGGGTGTGGGCCGGCCAAGGAGAGAGGGGGAGGCT
  CCGAGGACACCTTCTCTTCCTCCCTTCTTTTCAGACACGCAGCTTTCGGATTCAGATTCC
  TTTCTCGGACTTGCTGCTCCAGACTGAGGATGACGGCCACCGCCTGGTGGAGGATCAGGA
  GCTTGGTCTGGGGCTTGTCACTCTTGAGGTGGAGCTGCACCATGCGGCCGAGCTCTTTGA
  AAGCCTCGTTGATGTCACGGACCCGCAGACGCTCTCGGGCATTGTTGGCCATCCTCCGCT
  CCTTCTCACGCTCTGCCTTCTGCTCTGGTGTCAGGTCCTCATCGTCATTATTGCTAGTAA
  TTGATTTGATATCCTTCTTGTCGTCATCTAATTTCTTGTCCTCCGAAGATTTCGTGTCTT
  GCAAGTTCTCATCACCCTCGTCATCGGATTTGATCTCAGAGCTGCCAGAGGAGACACTCT
  GCCCCTGTAGTCCTGGTGGCATGCCTCTGNTAGGGTCCCTGGGGTGGGTTCAGGTCAGGG
  GAAGTCGCAAACTGGACAGNAAGCTGTGGAACCCGNACCTGNNNTTTGCAGAAAGAATGG
  CTGNCTCTCAGGNCCACGCATCTCACGATGGNTCCCACCATGANTGAATGTCTGTTGGCT
  GAAAAAAGGCCGTTCCATACCCTGAGCCCAGACANCCATGGCTCTTATGAGAAGTCCATG
  ATTCATGCTGTCCCTGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003199
• Insert Size: 2340 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003199.1, NP_003190.1
• RefSeq Size: 2500 bp
• RefSeq ORF: 2004 bp
• Locus ID: 6925
• Cytogenetics: 18q21.2
• Domains: HLH
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: 'This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Jul 2016]'
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence and uses an alternate in-frame splice site at the 3' end of an exon compared to variant 3. The resulting isoform (b, also known as TCF4-B-) is shorter at the N-terminus and lacks an alternate internal segment compared to isoform c. Sequence Note: This gene is distinct from TCF7L2 (alias TCF-4).