SPARC (NM_003118) Human Untagged Clone

CAT#: SC118167

SPARC (untagged)-Human secreted protein, acidic, cysteine-rich (osteonectin) (SPARC)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPARC
• Synonyms: BM-40; OI17; ON
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118167 sequence for NM_003118 edited (data generated by NextGen Sequencing)
  ATGAGGGCCTGGATCTTCTTTCTCCTTTGCCTGGCCGGGAGGGCCTTGGCAGCCCCTNNN
  NNAGAAGCCCTGCCTGATGAGACAGAGGTGGTGGAAGAAACTGTGGCAGAGGTGACTGAG
  GTATCTGTGGGAGCTAATCCTGTCCAGGTGGAAGTAGGAGAATTTGATGATGGTGCAGAG
  GAAACCGAAGAGGAGGTGGTGGCGGAAAATCCCTGCCAGAACCACCACTGCAAACACGGC
  AAGGTGTGCGAGCTGGATGAGAACAACACCCCCATGTGCGTGTGCCAGGACCCCACCAGC
  TGCCCAGCCCCCATTGGCGAGTTTGAGAAGGTGTGCAGCAATGACAACAAGACCTTCGAC
  TCTTCCTGCCACTTCTTTGCCACAAAGTGCACCCTGGAGGGCACCAAGAAGGGCCACAAG
  CTCCACCTGGACTACATCGGGCCTTGCAAATACATCCCCCCTTGCCTGGACTCTGAGCTG
  ACCGAATTCCCCCTGCGCATGCGGGACTGGCTCAAGAACGTCCTGGTCACCCTGTATGAG
  AGGGATGAGGACAACAACCTTCTGACTGAGAAGCAGAAGCTGCGGGTGAAGAAGATCCAT
  GAGAATGAGAAGCGCCTGGAGGCAGGAGACCACCCCGTGGAGCTGCTGGCCCGGGACTTC
  GAGAAGAACTATAACATGTACATCTTCCCTGTACACTGGCAGTTCGGCCAGCTGGACCAG
  CACCCCATTGACGGGTACCTCTCCCACACCGAGCTGGCTCCACTGCGTGCTCCCCTCATC
  CCCATGGAGCATTGCACCACCCGCTTTTTCGAGACCTGTGACCTGGACAATGACAAGTAC
  ATCGCCCTGGATGAGTGGGCCGGCTGCTTCGGCATCAAGCAGAAGGATATCGACAAGGAT
  CTTGTGATCTAA
  
  Clone variation with respect to NM_003118.2
  58 c=>n;59 a=>n;60 g=>n;61 c=>n;62 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003118 unedited
  GTCGCATTTGTATACGACTCCTATAGGGCGGCCGCGAATCGGCACCAGCTCCACATTCCC
  GCGGTCCTTCAGACTGCCCGGAGAGCGCGCTCTGCCTGCCGCCTGCCTGCCTGCCACTGA
  GGGTTCCCAGCACCATGAGGGCCTGGATCTTCTTTCTCCTTTGCCTGGCCGGGAGGGCCT
  TGGCAGCCCCTCAAGAAGCCCTGCCTGATGAGACAGAGGTGGTGGAAGAAACTGTGGCAG
  AGGTGACTGAGGTATCTGTGGGAGCTAATCCTGTCCAGGTGGAAGTAGGAGAATTTGATG
  ATGGTGCAGAGGAAACCGAAGAGGAGGTGGTGGCGGAAAATCCCTGCCAGAACCACCACT
  GCAAACACGGCAAGGTGTGCGAGCTGGATGAGAACAACACCCCCATGTGCGTGTGCCAGG
  ACCCCACCAGCTGCCCAGCCCCCATTGGCGAGTTTGAGAAGGTGTGCAGCAATGACAACA
  AGACCTTCGACTCTTCCTGCCACTTCTTTGCCACAAAGTGCACCCTGGAGGGCACCAAGA
  AGGGCCACAAGCTCCACCTGGACTACATCGGGCCTTGCAAATACATCCCCCCTTGCCTGG
  ACTCTGAGCTGACCGAATTCCCCCTGCGCATGCGGGACTGGCTCAAGAACGTCCTGGTCA
  CCCTGTATGAGAGGGATGAGGACAACAACCTTCTGACTGAGAAGCAGAAGCTGCGGGTGA
  AGAAGATCCATGAGAATGAGAAGCGCCTGGNAGGCAGAGACCACCCCGTGGAGCTGCTGG
  CCCCGGACTTCGAGAAGACTATAACATGTACATCCTCCCTGTACACTGGCAGTTCGGCCA
  GCTGGACCAGCACCCCATTGACGGGTACCTCTCCCACACCGAGCTGGCTCCATGCGTGCT
  CCCCTCATCCCATGGAGCATGCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003118 unedited
  CTATGGACCGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTGATTTTTTA
  GGAGCTTTTATTGTTTTAGTAATCTTAACATAACTTAAAATAAGAGAGGGGAAATGACAT
  CTGGAGATCTAGGTATGTGGCCCATTGCAATTGAGCACATTTCTTGGGTCTGTTTCTCTA
  TCTCTAAGGGGAGTCTCAAAACCCCAGCTCAAAATACGACACTAACATGATGAACATGCA
  TGAGCTTTGAAAAGTGCTCTGTAGTCTTATGATGATCTAGAAGAGCACTGTCCAATAGAA
  CTTTCTGTGATGATGAAAAGATTCTACTTCTGACCTATTCAATAGGGTAACCACTAATCA
  TGCATGGCTCTCAAGCACTTGAAATGTTGCTAGTGTGATTGGGGAGCTGCGTTTTGAATG
  TTAACTAATTTAAATTTAAATCTTAAAAAGTTACATGTGGGTAGTGGCTGCCGTACGGAA
  CAGTACAGCACTAGAATAATGCTGTGTCCTCCTGTTACACATACTCATGGTCTCTCCTAC
  TCTCCCTTCATCACACCTGTGACATCTTGCAATTTATATTTGATTGTGTCATTATCTAAT
  GTATTCCTCCTGGACTCTAAGCTCCAAATGAACAAATGCTTTGTCTACTTTGCCATAACT
  GTGTCCCCAGTGCTTGGCATGGGACCTGGTGCANAGTAAATACATCATAAATATTTTGTG
  AATGAATGAATGAATGAATGACCANNTGATAATAAAAGATATAGCTGCTCANTCTGGTGC
  TGATATTGGGGGANATGATCAAAAGGGTAAACCACAGGTCCTTAAATGAAAACAGCTAAC
  TTATTGCTTACAAGAAACCTACCCTCCCTGGGTATAAACTGCCCGGTGTTTGTGGTCCCG
  GGTGGTGTGTGCAAAAACATAATGGGGTTGCAAAGGGTGCATTTGGGGAACCATGGTAAA
  CAAGATGCCCTTGGGTTTTTGGGGAAGAAAATAAAGGAACTAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003118
• Insert Size: 3280 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003118.2, NP_003109.1
• RefSeq Size: 3178 bp
• RefSeq ORF: 912 bp
• Locus ID: 6678
• Cytogenetics: 5q33.1
• Domains: kazal, FOLN
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]'
  Transcript Variant: This variant (1) encodes the predominant isoform (1).