SRP54 (NM_003136) Human Untagged Clone

CAT#: SC118182

SRP54 (untagged)-Human signal recognition particle 54kDa (SRP54), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SRP54
• Synonyms: signal recognition particle 54kDa
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118182 sequence for NM_003136 edited (data generated by NextGen Sequencing)
  ATGGTTCTAGCAGACCTTGGAAGAAAAATAACATCAGCATTACGCTCGTTGAGCAATGCC
  ACCATTATCAATGAAGAGGTATTGAATGCTATGCTAAAAGAAGTCTGTACCGCTTTGTTG
  GAAGCAGATGTTAATATTAAACTAGTGAAGCAACTAAGAGAAAATGTTAAGTCTGCTATT
  GATCTTGAAGAGATGGCATCTGGTCTTAACAAAAGAAAAATGATTCAGCATGCTGTATTT
  AAAGAACTTGTGAAGCTTGTAGACCCTGGAGTTAAGGCATGGACACCCACTAAAGGAAAA
  CAAAATGTGATTATGTTTGTTGGATTGCAAGGGAGTGGTAAAACAACAACATGTTCAAAG
  CTAGCATATTATTACCAGAGGAAAGGTTGGAAGACCTGTTTAATATGTGCAGACACATTC
  AGAGCAGGGGCTTTTGACCAACTAAAACAGAATGCTACCAAAGCAAGAATTCCATTTTAT
  GGAAGCTATACAGAAATGGATCCTGTCATCATTGCTTCTGAAGGAGTAGAGAAATTTAAA
  AATGAAAATTTTGAAATTATTATTGTTGATACAAGTGGCCGCCACAAACAAGAAGACTCT
  TTGTTTGAAGAAATGCTTCAAGTTGCTAATGCTATACAACCTGATAACATTGTTTATGTG
  ATGGATGCCTCCATTGGGCAGGCTTGTGAAGCCCAGGCTAAGGCTTTTAAAGATAAAGTA
  GATGTAGCCTCAGTAATAGTGACAAAACTTGATGGCCATGCAAAAGGAGGTGGTGCACTC
  AGTGCAGTCGCTGCCACAAAAAGTCCGATTATTTTCATTGGTACAGGGGAACATATAGAT
  GACTTTGAACCTTTCAAAACACAGCCTTTTATTAGCAAACTTCTTGGTATGGGCGACATT
  GAAGGACTGATAGATAAAGTCAACGAGTTGAAGTTGGATGACAATGAAGCACTTATAGAG
  AAGTTGAAACATGGTCAGTTTACGTTGCGAGACATGTATGAGCAATTTCAAAATATCATG
  AAAATGGGCCCCTTCAGTCAGATCTTGGGGATGATCCCTGGTTTTGGGACAGATTTTATG
  AGCAAAGGAAATGAACAGGAGTCAATGGCAAGGCTAAAGAAATTAATGACAATAATGGAT
  AGTATGAATGATCAAGAACTAGACAGTACGGATGGTGCCAAAGTTTTTAGTAAACAACCA
  GGAAGAATCCAAAGAGTAGCAAGAGGATCGGGTGTATCAACAAGAGATGTTCAAGAACTT
  TTGACACAATATACCAAGTTTGCACAGATGGTAAAAAAGATGGGAGGTATCAAAGGACTT
  TTCAAAGGTGGCGACATGTCTAAGAATGTGAGCCAGTCACAGATGGCAAAATTGAACCAA
  CAAATGGCCAAAATGATGGATCCTAGGGTTCTTCATCACATGGGTGGTATGGCAGGACTT
  CAGTCAATGATGAGGCAGTTTCAACAGGGTGCTGCTGGCAACATGAAAGGCATGATGGGA
  TTCAATAATATGTAA
  
  Clone variation with respect to NM_003136.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003136 unedited
  TGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGCTCCGCCTAGTCACTTTT
  CTTAAGTGGCTCGTCGAGGCCTGACTTCTTCCCCGAATCACGTCCCTAGACAGCCTCCTA
  TTTTACCACTAACTTTACTCCTGCAGTTATTCAGCGGTAGGAAACTGAAACCAAAAACCA
  GTGTAAGCAAGTAAACATCTAAACTGTTTCAGGAGCCGCGTAGAAGGAACGCGGCGGTGT
  GCCCCGGAAGCGGAAGTAGATTCTCCTATAGAAAGGCTGGACTACGCGGAGTGGTGACGT
  TTCCTCATTGGGCGGAAGGTTCGCTGGCACTCCGTTGGTCTTCCAGCTGGTGGGAGTTGA
  CGACGTGGTGCTGGGCGTTGGGACCCTACTTTATCTAGTTCGGGAAGTTGGGTTGTGGGG
  TCATACCTGTCTGTCTGCTCCCAGCTTTCTTGGGTTTCTTCCGACGGCGTGGGGCCTCGC
  TAAGGAATTCCCGGCCCCTCAGGGCCACGGCTTTAGCGGTGTCTTTTGCGAGTTCTTCGT
  AAGTACATCTTAAAGCTGTCAAGATGGTTCTAGCAGACCTTGGAAGAAAAATAACATCAG
  CATTCCGCTCGTTGAGCAATGCCACCATTATCAATGAAGAGGTATTGAATGCTATGCTAA
  AAGAAGTCTGTACCGCTTTGTTGGAAGCAGATGTTAATATTAAACTAGTGAAGCAACTAA
  AGAAATGTTAAGTCTGCTATTGATCTTGAAGAGATGCATCTGGTCTTAACAAAGAAAATG
  ATTCAGCATGCTGTATTAAAGAACTTGTGAAGCTGTAACCCTGNANTNAGGCATGGACAC
  CACTAAAGAAACANATGTGATATATGTTGTTGGATGCAAGGGAGTGGTAAACAANCACTG
  NTCAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003136 unedited
  NCCCCCACGCCCTCCNCCCCCCCCCCCCNNCCTTTCNCTTGTTTTGCNACGCGCGCCGCC
  TCCCACGATCGAGGTTCTTTTCTCTTTTTTTTCTTTTTATTCTACAGCACTTTAATTGAA
  ACATTATTTAACTACTGACCAGGACTGTTTACAAATGCAATGCTTGGCCTTCGAGATAAT
  TAAAAACCTTTCAGTACTAAAATTTTACATCATGATTTAACTTAAGTGTTACGATGGTGA
  AAACAGAACACAATCTAAAGCACACATATAATGATTTCCACACAAACCATGTATTTCTCC
  CTTATATTAAAGGGAGGAACCGATGAAGGAAAAAGCAAACGGAGGGGAAAAGCGGGCGAG
  ATAAAAAGGAAAGAGTGCATGATAAGCAACAAAAATACACTTTCCCCCCAATTCGCACAA
  AGAATGGAAACGCTGAGGTCTCCACCCAAATTAGGTATCCAACTGAGTCAGTTTATATTA
  AGGCATTTCCTTTACATATTATTGAAATCCCATCATGCCTTTCATGTTGCCAGCAGAACC
  CTGCTGAAACTGCCTCACCATTGACTGAAGTCTTGCCCTACCACCCCATGTGATGAAAAA
  ACCCTAGGATTCATTCATTTTTTGGCCATTCGTTGGTTCACATTTGCATCTGTGACTGCC
  TCACATTTTTACACCATGTTCGCACCTTTTGAAAGCCCTTTTGATACCTCCCATTTTTTT
  TTACAATCTGCGCAAACTTGGTAACACTGCGACAAAAGCTTTTGGAACAACCTCTGCTGA
  AACACCCGATCCTTTCGCTCCCCTTTGGATCCTCNCCGGCCGTTCACTCAAATCTTTTGG
  CACTTCCCGTCCGGCCATACCTTTGAACTTCCTAACACGCCATTTTCGTCCTTAATTTTT
  TTAACCTCTGCCTTGAATCCCTGGCATATTCCTTTGGCTCAATACACCTTGCCCAAAACA
  CCGCGTATATCCCCAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003136
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003136.2, NP_003127.1
• RefSeq Size: 2164 bp
• RefSeq ORF: 1515 bp
• Locus ID: 6729
• Cytogenetics: 14q13.2
• Domains: SRP54, AAA, SRP_SPB
• Protein Pathways: Protein export
• Gene Summary: ''
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).