TRIM21 (NM_003141) Human Untagged Clone

CAT#: SC118185

TRIM21 (untagged)-Human tripartite motif containing 21 (TRIM21)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRIM21
• Synonyms: RNF81; Ro/SSA; RO52; SSA; SSA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003141, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTTCAGCAGCACGCTTGACAATGATGTGGGAGGAGGTCACATGCCCTATCTGCCTGGACCCCTTCG
  TGGAGCCTGTGAGCATCGAGTGTGGCCACAGCTTCTGCCAGGAATGCATCTCTCAGGTTGGGAAAGGTGG
  GGGCAGCGTCTGTCCTGTGTGCCGGCAGCGCTTTCTGCTCAAGAATCTCCGGCCCAATCGACAGCTAGCC
  AACATGGTGAACAACCTTAAAGAAATCAGCCAGGAGGCCAGAGAGGGCACACAGGGGGAACGGTGTGCAG
  TGCATGGAGAGAGACTTCACCTGTTCTGTGAGAAAGATGGGAAGGCCCTTTGCTGGGTATGTGCCCAGTC
  TCGGAAACACCGTGACCACGCCATGGTCCCTCTTGAGGAGGCTGCACAGGAGTACCAGGAGAAGCTCCAG
  GTGGCATTAGGGGAACTGAGAAGAAAGCAGGAGTTGGCTGAGAAGTTGGAAGTGGAAATTGCAATAAAGA
  GAGCAGACTGGAAGAAAACAGTGGAAACACAGAAATCTAGGATTCACGCAGAGTTTGTGCAGCAAAAAAA
  CTTCCTGGTTGAAGAAGAACAGAGGCAGCTGCAGGAGCTGGAGAAGGATGAGAGGGAGCAGCTGAGAATC
  CTGGGGGAGAAAGAGGCCAAGCTGGCCCAGCAGAGCCAGGCCCTACAGGAGCTCATCTCAGAGCTAGATC
  GAAGGTGCCACAGCTCAGCACTGGAACTGCTGCAGGAGGTGATAATTGTCCTGGAAAGGAGTGAGTCCTG
  GAACCTGAAGGACCTGGATATTACCTCTCCAGAACTCAGGAGTGTGTGCCATGTGCCAGGGCTGAAGAAG
  ATGCTGAGGACATGTGCAGTCCACATCACTCTGGATCCAGACACAGCCAATCCGTGGCTGATACTTTCAG
  AAGATCGGAGACAAGTGAGGCTTGGAGACACCCAGCAGAGCATACCTGGAAATGAAGAGAGATTTGATAG
  TTATCCTATGGTCCTGGGTGCCCAGCACTTTCACTCTGGAAAACATTACTGGGAGGTAGATGTGACAGGA
  AAGGAGGCCTGGGACCTGGGTGTCTGCAGAGACTCTGTGCGCAGGAAGGGGCACTTTTTGCTTAGTTCCA
  AGAGTGGCTTCTGGACAATTTGGTTGTGGAACAAACAAAAATATGAGGCTGGCACCTACCCCCAGACTCC
  CCTCCACCTTCAGGTGCCTCCATGCCAAGTTGGGATTTTCCTGGACTATGAGGCTGGCATGGTCTCCTTC
  TACAACATCACTGACCATGGCTCCCTCATCTACTCCTTCTCTGAATGTGCCTTTACAGGACCTCTGCGGC
  CCTTCTTCAGTCCTGGTTTCAATGATGGAGGAAAAAACACAGCCCCTCTAACCCTCTGTCCACTGAATAT
  TGGATCACAAGGATCCACTGACTATTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003141 unedited
  CGCCCCCCAGAANNAAACACACCACCCNTCCGCGGTTCATAATTGTATAAGACTCACTAT
  AGGCGGCCGCGAAATTCGCACGAGGTCCCCTGTAAAGCCAAACCCCCTAAAGTCTCCACA
  CTGCTGTTTAACGGCACACTTGACAATGGCTTCAGCAGCACGCTTGACAATGATGTGGGA
  GGAGGTCACATGCCCTATCTGCCTGGACCCCTTCGTGGAGCCTGTGAGCATCGAGTGTGG
  CCACAGCTTCTGCCAGGAATGCATCTCTCAGGTTGGGAAAGGTGGGGGCAGCGTCTGTCC
  TGTGTGCCGGCAGCGCTTTCTGCTCAAGAATCTCCGGCCCAATCGACAGCTAGCCAACAT
  GGTGAACAACCTTAAAGAAATCAGCCAGGAGGCCAGAGAGGGCACACAGGGGGAACGGTG
  TGCAGTGCATGGAGAGAGACTTCACCTGTTCTGTGAGAAAGATGGGAAGGCCCTTTGCTG
  GGTATGTGCCCAGTCTCGGAAACACCGTGACCACGCCATGGTCCCTCTTGAGGAGGCTGC
  ACAGGAGTACCAGGAGAAGCTCCAGGTGGCATTAGGGGAACTGAGAAGAAAGCAGGAGTT
  GGCTGAGAAGTTGGAAGTGGAAATTGCAATAAAGAGAGCAGACTGGAAGAAAACAGTGGA
  AACACAGAAATCTAGGATTCACGCAGAGTTTGTGCAGCANAAAAACTTCCTGTTTGAAGA
  AGAACCAGAGCAGCTGCANGAACTGGAGAAGGATGACAGGGAGCACCTGAGAATCCTGCG
  GGAGAAAGAGCCCAGCTGGCCCAGCAGAGCCAGCCCCTACAGGAGCTCATCTCAGAGCTA
  GATCCGAAGTGCCACAGCTCAACACTGGAACTGCTGCAGGAGTGATTATTGTCCTGGAAG
  GAGTGGTCCTGCAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003141 unedited
  GGGAAACTTAGGAACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTAAATTTTATTTTTTATTTTTAGGAAGCCCTTTTGCAAACCATACAAG
  ATATAATAGAGATCTCACCAAAGACGACATCCTAGAGATGGAGAGGAGAAAAAAAAAACT
  TAAGTCCCATAAAGAAGGCAGAAACATGTTTTTGGTTGATCAAGATGAGTTTGGGCCAAA
  TATAAGGAGGCTGCTTCACTGGAGGGAATCACAAAGCCATCTGGGGCAGGAATGTTGATG
  GTGAAGTGACTTCCCTGCTAAAGCTCGCTTGCTGGGATCCGGATGCCTCTGCAGAGACAA
  AGGTGGTTCAGAGTTCATGGGGAAAAGAGGCAGGGTTTGTGGCTGAGAAGCGGTGCCAGT
  GGGGAGAGTGGCAGTGTCCAGAGAAAGCCATCAATAGTCAGTGGATCCTTGTGATCCAAT
  ATTCAGTGGACAGAGGGTTAGAGGGGCTGTGTTTTTTCCTCCATCATTGAAACCAGGACT
  GAAGAAGGGCCGCAGAGGTCCTGTAAAGGCACATTCAGAGAAGGAGTAGATGAGGGAGCC
  ATGGTCAGTGATGTTGTAGAAGGAGACCATGCCAGCCTCATAGTCCAGGGAAATCCCAAC
  CTGGCCGGGGAGGCCCCGGAGGTGGGGGGGAATCCGGGGGTAAGTGCACNCCCCCAAGTT
  TTGTGGGGCCCACAGCCAANNTGCCACCAACCACCCCTGGACAACAGCAAAGGGGAGCNN
  NNNGCGGCACTGAGGCCGGNNAAACACTGAGGNNNAAGGGGCGCTTTGCCGGTGNNAGCG
  AGGTGCGGGGNNNNNTAGCACAGAGGCAAATGGGCGGGNNCTCAGCGGACCAGGGAAAAC
  GAGAA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_003141
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003141.3, NP_003132.2
• RefSeq Size: 1946 bp
• RefSeq ORF: 1428 bp
• Locus ID: 6737
• Cytogenetics: 11p15.4
• Domains: zf-B_box, RING, SPRY, PRY
• Protein Families: Druggable Genome
• Protein Pathways: Systemic lupus erythematosus
• Gene Summary: 'This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]'