SSBP1 (NM_003143) Human Untagged Clone

CAT#: SC118188

SSBP1 (untagged)-Human single-stranded DNA binding protein 1 (SSBP1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SSBP1
• Synonyms: Mt-SSB; mtSSB; SOSS-B1; SSBP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118188 sequence for NM_003143 edited (data generated by NextGen Sequencing)
  ATGTTTCGAAGACCTGTATTACAGGTACTTCGTCAGTTTGTAAGACATGAGTCCGAAACA
  ACTACCAGTTTGGTTCTTGAAAGATCCCTGAATCGTGTGCACTTACTTGGGCGAGTGGGT
  CAGGACCCTGTCTTGAGACAGGTGGAAGGAAAAAATCCAGTCACAATATTTTCTCTAGCA
  ACTAATGAGATGTGGCGATCAGGGGATAGTGAAGTTTACCAACTGGGTGATGTCAGTCAA
  AAGACAACATGGCACAGAATATCAGTATTCCGGCCAGGCCTCAGAGACGTGGCATATCAA
  TATGTGAAAAAGGGGTCTCGAATTTATTTGGAAGGGAAAATAGACTATGGTGAATACATG
  GATAAAAATAATGTGAGGCGACAAGCAACAACAATCATAGCTGATAATATTATATTTCTG
  AGTGACCAGACGAAAGAGAAGGAGTAG
  
  Clone variation with respect to NM_003143.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003143 unedited
  GGGTCATATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGCGGAGTGA
  TCCCCTGCGTGGCTGGGCTGCTCGGGTTAGATCGTCAGGAAAAGCCTAAAGATTAGACTG
  TAAGAAAAGAAAATAGAAGCCATGTTTCGAAGACCTGTATTACAGGTACTTCGTCAGTTT
  GTAAGACATGAGTCCGAAACAACTACCAGTTTGGTTCTTGAAAGATCCCTGAATCGTGTG
  CACTTACTTGGGCGAGTGGGTCAGGACCCTGTCTTGAGACAGGTGGAAGGAAAAAATCCA
  GTCACAATATTTTCTCTAGCAACTAATGAGATGTGGCGATCAGGGGATAGTGAAGTTTAC
  CAACTGGGTGATGTCAGTCAAAAGACAACATGGCACAGAATATCAGTATTCCGGCCAGGC
  CTCAGAGACGTGGCATATCAATATGTGAAAAAGGGGTCTCGAATTTATTTGGAAGGGAAA
  ATAGACTATGGTGAATACATGGATAAAAATAATGTGAGGCGACAAGCAACAACAATCATA
  GCTGATAATATTATATTTCTGAGTGACCAGACGAAAGAGAAGGAGTAGAAAGGATGATTC
  TTCTTTGGCCATCATTTGGTACAGTCTCATTTCCAAGTCATGTATAATCTTTATGGCTTC
  CAAGGACAAGAATTAAAATACTCTTTTACGTAAAAAAAAAAAAAAAAAAAACTCGACTCT
  AGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGACCCCGGTGGCATTCCTGTGACCC
  CTTCCCATGCCTCTCCTGGCCCTGAAGTTGCCACTTCAGTGCCCACAGCCTTGTCCTATA
  AAATTAAGTGCATCATTTTGTCTGACTAGGTGTCCTTTATAATATTATGGGGTGGAGGGG
  GGGGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003143 unedited
  GCTATGGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTACGTAAA
  AGATATTTTAATTCTTGTCCTTGGAAGCCATAAAGATTATACATGACTTGGAAATGAGAC
  TGTACCAAATGATGGCCAAAGAAGAATCATCCTTTCTACTCCTTCTCTTTCGTCTGGTCA
  CTCAGAAATATAATATTATCAGCTATGATTGTTGTTGCTTGTCGCCTCACATTATTTTTA
  TCCATGTATTCACCATAGTCTATTTTCCCTTCCAAATAAATTCGAGACCCCTTTTTCACA
  TATTGATATGCCACGTCTCTGAGGCCTGGCCGGAATACTGATATTCTGTGCCATGTTGTC
  TTTTGACTGACATCACCCAGTTGGTAAACTTCACTATCCCCTGATCGCCACATCTCATTA
  GTTGCTAGAGAAAATATTGTGACTGGATTTTTTCCTTCCACCTGTCTCAAGACAGGGTCC
  TGACCCACTCGCCCAAGTAAGTGCACACGATTCAGGGATCTTTCAAGAACCAAACTGGTA
  GTTGTTTCGGACTCATGTCTTACAAACTGACGAAGTACCTGTAATACAGGTCTTCGAAAC
  ATGGCTTCTATTTTCTCTTCTTACAGNCTAATCTTTANGCTCTTCCTGACGATCTACCCC
  GAGCCGCCCACCACGCAGGGGATCACTTCCGCCTNNGGCCGAATCNNGGGCCGCCTATAG
  TGAGTCGTATTACAAATCCTGACGGGTCACTAAACGAGCCCTGCTTATATAAACCCCCCA
  CCGGACACGCCCTAACGCCCATTTGTGGTNAACGGGGGGGGGTTATACACANATTCTGAA
  AAGNCCCGTTGCTTTGGGGCCCAACCAACCCGCCTGGCNNNCTGGCGCGGGGGTGCGAAA
  TCCNNGAGAGCCCNNCCCTTCGGGCCTTGGCGGGCCGNCAACCCCTACTCGAGTGGCGNG
  CCGCAACCCAAGCGCGGGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003143
• Insert Size: 690 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003143.1, NP_003134.1
• RefSeq Size: 628 bp
• RefSeq ORF: 447 bp
• Locus ID: 6742
• Cytogenetics: 7q34
• Domains: SSB
• Protein Families: Druggable Genome
• Protein Pathways: DNA replication, Homologous recombination, Mismatch repair
• Gene Summary: 'SSBP1 is a housekeeping gene involved in mitochondrial biogenesis (Tiranti et al., 1995 [PubMed 7789991]). It is also a subunit of a single-stranded DNA (ssDNA)-binding complex involved in the maintenance of genome stability (Huang et al., 2009) [PubMed 19683501].[supplied by OMIM, Feb 2010]'
  Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. Variants 1 through 5 encode the same protein.