SNRPN (NM_003097) Human Untagged Clone

CAT#: SC118198

SNRPN (untagged)-Human small nuclear ribonucleoprotein polypeptide N (SNRPN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SNRPN
• Synonyms: HCERN3; PWCR; PWS; RT-LI; SM-D; sm-N; SMN; SNRNP-N; SNURF-SNRPN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118198 sequence for NM_003097 edited (data generated by NextGen Sequencing)
  ATGACTGTTGGCAAGAGTAGCAAGATGCTGCAGCACATTGACTATAGAATGAGATGTATC
  CTGCAAGATGGCCGAATCTTCATTGGCACCTTTAAGGCTTTTGACAAGCATATGAATTTG
  ATCCTCTGTGATTGTGATGAGTTCAGAAAGATCAAGCCAAAGAATGCGAAGCAACCAGAG
  CGTGAAGAAAAGCGGGTTTTGGGTCTGGTGTTGCTGCGTGGGGAGAACTTGGTATCCATG
  ACTGTGGAGGGGCCACCCCCCAAAGATACTGGCATTGCTCGGGTACCACTTGCTGGAGCT
  GCTGGAGGCCCTGGGGTTGGTAGGGCAGCTGGTAGAGGAGTACCAGCTGGTGTGCCAATT
  CCCCAGGCCCCTGCTGGATTGGCAGGCCCTGTCCGAGGAGTTGGGGGACCATCCCAGCAG
  GTAATGACTCCACAGGGAAGAGGCACTGTAGCAGCTGCTGCTGTTGCTGCGACTGCCAGT
  ATTGCTGGAGCCCCAACACAGTACCCACCAGGACGGGGCACTCCGCCCCCACCCGTCGGC
  AGAGCAACCCCACCTCCAGGCATTATGGCTCCTCCACCTGGTATGAGACCACCCATGGGC
  CCACCAATTGGGCTTCCCCCTGCTCGAGGGACGCCAATAGGCATGCCGCCTCCGGGAATG
  AGACCCCCTCCACCAGGCATTAGAGGTCCACCTCCCCCAGGAATGCGTCCACCAAGACCT
  TAG
  
  Clone variation with respect to NM_003097.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003097 unedited
  NGGGTGCGAATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGGCAAGG
  CCAGCCGCCCCCTGTCTTCCCTCTGGAGCTGTTTGAGGAATGCAGTTCTTTTGCATAAGA
  AAGGCTTTTCTCTCCAGTCATTCTGCTTGCTGATCAAGAACACTTAACAGCTTCAGGCTT
  TCCTGATACTTTGACGAAGGTAATTGGGACTCCCATCAAGTCTCCACACAGCTAGCAGCC
  ACGTGGGGCACTTCTCCAGCATATGTAAGTGGAACTCAGAAGGCAACATTCCCTGAACAT
  ACTCTTCCACCAGAATCTCCTCTACAGATTTTTGCTGCCCCTTTTACCAGTGGCTGAATC
  TACTTTCTCTTATGTTTTGCTCCATCACCCAGGCGGAGTGCAGTGGTGCCATCACAGCTT
  ACTGCAGTCTCCACCTCTCAGGCTCAAGTGATTCTCCTACCTCAGCCTCCCGAGTAGCTG
  GGACCAGAGGGATCGCTTACACCTGAGACGAACTACAGAACAGCACGTACCAGAGGTGGA
  AGTCCAAGTCAAACGCAGAAGGACTGCCTCACTGAGCAACCAAGAGTGTCAGTTGTACCC
  GAGGCGTTCTCAGCAGCAGCAAGTACCTGTGGTGGATTTCCAGGCTGAACTGAGGCAGGC
  ATTCTTAGCTGAGACACCAAGAGGTGGTTAAAGCCATATTGGAGTAGCGAGGAATCTGAT
  TCCAAGCAAAAACCAGGCTCCATCTACTCTNTGAAGCTTCTGCCCAGCTTGCATTGNTTC
  TAGGAGACCTGCGTCATACCTTTATCTATAGCCCTCCCCCTAGTCTTCAGAAGCATCAAG
  TTTTAACTGTGGACATTGANATTGNTGGNAACAGCATCATGACTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003097 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACAGTTTAATTGCTCTATG
  CATTTATTATTAGCTATTATTAATGCAGAATGAGGGAACAAAAAGCTCACAAACACTCTA
  CACAATTTCACAAGACGCATTGCAGGGGAAAAAGTGACTGAGATGGATCAACAGTATGCT
  AAGGTCTTGGTGGACGCATTCCTGGGGGAGGTGGACCTCTAATGCCTGGTGGAGGGGGTC
  TCATTCCCGGAGGCGGCATGCCTATTGGCGTCCCTCGAGCAGGGGGAAGCCCAATTGGTG
  GGCCCATGGGTGGTCTCATACCAGGTGGAGGAGCCATAATGCCTGGAGGTGGGGTTGCTC
  TGCCGACGGGTGGGGGCGGAGTGCCCCGTCCTGGTGGGTACTGTGTTGGGGCTCCAGCAA
  TACTGGCAGTCGCAGCAACAGCAGCAGCTGCTACAGTGCCTCTTCCCTGTGGAGTCATTA
  CCTGCTGGGATGGTCCCCCAACTCCTCGGACAGGGCCTGCCAATCCAGCAGGGGCCTGGG
  GAATTGGCACACCAGCTGGTACTCCTCTACCAGCTGCCCTACCAACCCCAGGGCCTCCAG
  CAGCTCCAGCAAGTGGTACCCGAGCAATGCCAGTATCTTTGGGGGGTGGCCCCTCCACAG
  TCATGGATACCAAGTTCTCCCCACGCAGCAACACCAGACCCAAAACCCGCTTTTCTTCAC
  GCTCTGGTTGCTTCGCATTCTTTGGCTTGATCTTTCTGAACTCATCACAATCACAGAGGA
  TCAAATTCATATGCTTGTCAAAAGCCTTNAAGGGTGCCATGAAGATTCNGCCATCTTGCA
  GATACATCTCATTCTTAGTNAATGTGCTGCACATCCTGCTACTCTGCCACAGTCATGATG
  CTGTCCACCAATCCATGTCCCAGNTAAACTTGAGCTCTGAGACCTAGGGAAGCTTAGATA
  AGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003097
• Insert Size: 1640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003097.3, NP_003088.1
• RefSeq Size: 1326 bp
• RefSeq ORF: 723 bp
• Locus ID: 6638
• Cytogenetics: 15q11.2
• Domains: Sm
• Protein Families: Stem cell - Pluripotency
• Gene Summary: 'This gene is located within the Prader-Willi Syndrome critical region on chromosome 15 and is imprinted and expressed from the paternal allele. It encodes a component of the small nuclear ribonucleoprotein complex, which functions in pre-mRNA processing and may contribute to tissue-specific alternative splicing. Alternative promoter use and alternative splicing result in a multitude of transcript variants encoding the same protein. Transcript variants that initiate at the CpG island-associated imprinting center may be bicistronic and also encode the SNRPN upstream reading frame protein (SNURF) from an upstream open reading frame. In addition, long spliced transcripts for small nucleolar RNA host gene 14 (SNHG14) may originate from the promoters at this locus and share exons with this gene. Alterations in this region are associated with parental imprint switch failure, which may cause Angelman syndrome or Prader-Willi syndrome. [provided by RefSeq, Mar 2017]'
  Transcript Variant: This variant (1) is the predominant splice variant and initiates at the imprinting center. This splice variant is bicistronic and can also encode the SNURF protein from an upstream open reading frame. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.