SLUG (SNAI2) (NM_003068) Human Untagged Clone

CAT#: SC118205

SNAI2 (untagged)-Human snail homolog 2 (Drosophila) (SNAI2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SNAI2
• Synonyms: SLUG; SLUGH; SLUGH1; SNAIL2; WS2D
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003068 edited
  GAATTCGGCACGAGGAGGAGCCGGGTGACTTCAGAGGCGCCGGCCCGTCCGTCTGCCGCA
  CCTGAGCACGGCCCCTGCCCGAGCCTGGCCCGCCGCGATGCTGTAGGGACCGCCGTGTCC
  TCCCGCCGGACCGTTATCCGCGCCGGGCGCCCGCCAGACCCGCTGGCAAGATGCCGCGCT
  CCTTCCTGGTCAAGAAGCATTTCAACGCCTCCAAAAAGCCAAACTACAGCGAACTGGACA
  CACATACAGTGATTATTTCCCCGTATCTCTATGAGAGTTACTCCATGCCTGTCATACCAC
  AACCAGAGATCCTCAGCTCAGGAGCATACAGCCCCATCACTGTGTGGACTACCGCTGCTC
  CATTCCACGCCCAGCTACCCAATGGCCTCTCTCCTCTTTCCGGATACTCCTCATCTTTGG
  GGCGAGTGAGTCCCCCTCCTCCATCTGACACCTCCTCCAAGGACCACAGTGGCTCAGAAA
  GCCCCATTAGTGATGAAGAGGAAAGACTACAGTCCAAGCTTTCAGACCCCCATGCCATTG
  AAGCTGAAAAGTTTCAGTGCAATTTATGCAATAAGACCTATTCAACTTTTTCTGGGCTGG
  CCAAACATAAGCAGCTGCACTGCGATGCCCAGTCTAGAAAATCTTTCAGCTGTAAATACT
  GTGACAAGGAATATGTGAGCCTGGGCGCCCTGAAGATGCATATTCGGACCCACACATTAC
  CTTGTGTTTGCAAGATCTGCGGCAAGGCGTTTTCCAGACCCTGGTTGCTTCAAGGACACA
  TTAGAACTCACACGGGGGAGAAGCCTTTTTCTTGCCCTCACTGCAACAGAGCATTTGCAG
  ACAGGTCAAATCTGAGGGCTCATCTGCAGACCCATTCTGATGTAAAGAAATACCAGTGCA
  AAAACTGCTCCAAAACCTTCTCCAGAATGTCTCTCCTGCACAAACATGAGGAATCTGGCT
  GCTGTGTAGCACACTGAGTGACGCAATCAATGTTTACTCGAACAGAATGCATTTCTTCAC
  TCCGAAGCCAAATGACAAATAAAGTCCAAAGGCATTTTCTCCTGTGCTGACCAACCAAAT
  AATATGTATAGACACACACACATATGCACACACACACACACACACCCACAGAGAGAGAGC
  TGCAAGAGCATGGAATTCATGTGTTTAAAGATAATCCTTTCCATGTGAAGTTTAAAATTA
  CTATATATTTGCTGATGGCTAGATTGAGAGAATAAAAGACAGTAACCTTTCTCTTCAAAG
  ATAAAATGAAAAGCACATTGCATCTTTTCTTCCTAAAAAAATGCAAAGATTTACATTGCT
  GCCAAATCATTTCAACTGAAAAGAACAGTATTGCTTTGTAATAGAGTCTGTAATAGGATT
  TCCCATAGGAAGAGATCTGCCAGACGCGAACTCAGGTGCCTTAAAAAGTATTCCAAGTTT
  ACTCCATTACATGTCGGTTGTCTGGTTGCCATTGTTGAACTAAAGCCTTTTTTTGATTAC
  CTGTAGTGCTTTAAAGTATATTTTTAAAAGGGAGGAAAAAAATAACAAGAACAAAACACA
  GGAGAATGTATTAAAAGTATTTTTGTTTTGTTTTGTTTTTGCCAATTAACAGTATGTGCC
  TTGGGGGAGGAGGGAAAGATTAGCTTTGAACATTCCTGGCGCATGCTCCATTGTCTTACT
  ATTTTAAAACATTTTAATAATTTTTGAAAATTAATTAAAGATGGGAATAAGTGCAAAAGA
  GGATTCTTACAAATTCATTAATGTACTTAAACTATTTCAAATGCATACCACAAATGCAAT
  AATACAATACCCCTTCCAAGTGCCTTTTTAAATTGTATAGTTGATGAGTCAATGTAAATT
  TGTGTTTATTTTTATATGATTGAATGAGTTCTGTATGAAACTGAGATGTTGTCTATAGCT
  ATGTCTATAAACAACCTGAAGACTTGTGAAATCAATGTTTCTTTTTTAAAAAACAATTTT
  CAAGTTTTTTTTACAATAAACAGTTTTGATTTAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003068 unedited
  GTCAGAATTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGAGGAGCCGGG
  TGACTTCAGAGGCGCCGGCCCGTCCGTCTGCCGCACCTGAGCACGGCCCCTGCCCGAGCC
  TGGCCCGCCGCGATGCTGTAGGGACCGCCGTGTCCTCCCGCCGGACCGTTATCCGCGCCG
  GGCGCCCGCCAGACCCGCTGGCAAGATGCCGCGCTCCTTCCTGGTCAAGAAGCATTTCAA
  CGCCTCCAAAAAGCCAAACTACAGCGAACTGGACACACATACAGTGATTATTTCCCCGTA
  TCTCTATGAGAGTTACTCCATGCCTGTCATACCACAACCAGAGATCCTCAGCTCAGGAGC
  ATACAGCCCCATCACTGTGTGGACTACCGCTGCTCCATTCCACGCCCAGCTACCCAATGG
  CCTCTCTCCTCTTTCCGGATACTCCTCATCTTTGGGGCGAGTGAGTCCCCCTCCTCCATC
  TGACACCTCCTCCAAGGACCACAGTGGCTCAGAAAGCCCCATTAGTGATGAAGAGGAAAG
  ACTACAGTCCAAGCTTTCAGACCCCCATGCCATTGAAGCTGAAAAGTTTCAGTGCAATTT
  ATGCAATAAGACCTATTCAACTTTTTCTGGGCTGGCCAAACATAAGCAGCTGCACTGCGA
  TGCCCAGTCTAGAAAATCTTTCAGCTGTAAATACTGTGACAAGGAATATGTGAGCCTGGG
  CGCCCTGAAGATGCATATTCGGACCCACACATTACCTTGTGTTTGCAAGATCTGCGGNCA
  GGCGTTTTCCAGACCCTGGTTGCTTCAAGACACATTAGAACTCACACGGNGGAGAAGCCT
  TTTTCTTGCNCCTCACTGCACAGAGCATTTGCAGACAGGTCAAATCTGAGGGCTCATCTG
  CAGACCCATTNCTGATGTAAGAAATACCAGTGCANAAACTGCTNCCACACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003068 unedited
  GCGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTAAATCAAAACTGTTTATTG
  TAAAAAAAACTTGAAAATTGTTTTTTAAAAAAAAAACATTGATTTCACAAGTCTTCAGGT
  TGTTTATAGACATAGCTATAGACAACATCTCAGTTTCATACAGAACTCATTCAATCATAT
  AAAAATAAACACAAATTTACATTGACTCATCAACTATACAATTTAAAAAGGCACTTGGAA
  GGGGTATTGTATTATTGCATTTGTGGTATGCATTTGAAATAGTTTAAGTACATTAATGAA
  TTTGTAAGAATCCTCTTTTGCACTTATTCCCATCTTTAATTAATTTTCAAAAATTATTAA
  AATGTTTTAAAATAGTAAGACAATGGAGCATGCGCCAGGAATGTTCAAAGCTAATCTTTC
  CCTCCTCCCCCAAGGCACATACTGTTAATTGGCAAAAACAAAACAAAACAAAAATACTTT
  TAATACATTCTCCTGAGTTTTGTTCTTGTTATTTTTTTCCTCCCTTTTAAAAATATACTT
  TAAAGCACTACAGGTAATCAAAAAAAGGCTTTAGTTCAACAATGGCAACCAGACAACCGA
  CATGTAATGGAGTAAACTTGGAATACTTTTTAAGGCACCTGAGTTCGCGTCTGGCAGATC
  TCTTCCTATGGGAAATCCTATTACAGACTCTATTACAAAGCAATACTGTTCTTTTCAGTT
  GAAATGATTTGGCAGCAATGTAAATCTTTGCATTNTTTTAGGAAGAAAAAGATGCATGTG
  CTTTTCATTTTATCTTTGAAGAGAAAGTTACTGTNCTTTATTCTCTCAATCTAGCCATCA
  GCAAATATATAGTAATTTTAAACTTACATGGGAAGGGATATCTTAACACATGAAATTCAT
  GCTCTGCAGCTCTCTTCTGGGGNNGGNGNGNGGGGGNGGGCATATGGGGGGNGNCTATCA
  TATTATTGGGTGGNCANACAGGAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003068
• Insert Size: 1900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003068.3, NP_003059.1
• RefSeq Size: 2101 bp
• RefSeq ORF: 807 bp
• Locus ID: 6591
• Cytogenetics: 8q11.21
• Domains: zf-C2H2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adherens junction
• Gene Summary: 'This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008]'