SLC10A1 (NM_003049) Human Untagged Clone

CAT#: SC118232

SLC10A1 (untagged)-Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC10A1
• Synonyms: NTCP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118232 sequence for NM_003049 edited (data generated by NextGen Sequencing)
  ATGGAGGCCCACAACGCGTCTGCCCCATTCAACTTCACCCTGCCACCCAACTTTGGCAAG
  CGCCCCACAGACCTGGCACTGAGCGTCATCCTGGTGTTCATGTTGTTCTTCATCATGCTC
  TCGCTGGGCTGCACCATGGAGTTCAGCAAGATCAAGGCTCACTTATGGAAGCCTAAAGGG
  CTGGCCATCGCCCTGGTGGCACAGTATGGCATCATGCCCCTCACAGCCTTTGTGCTGGGC
  AAGGTCTTCCGGCTGAAGAACATTGAGGCACTGGCCATCTTGGTCTGTGGCTGCTCACCT
  GGAGGGAACCTGTCCAATGTCTTCAGTCTGGCCATGAAGGGGGACATGAACCTCAGCATT
  GTGATGACCACCTGCTCCACCTTCTGTGCCCTTGGCATGATGCCTCTCCTCCTGTACATC
  TACTCCAGGGGGATCTATGATGGGGACCTGAAGGACAAGGTGCCCTATAAAGGCATCGTG
  ATATCACTGGTCCTGGTTCTCATTCCTTGCACCATAGGGATCGTCCTCAAATCCAAACGG
  CCACAATACATGCGCTATGTCATCAAGGGAGGGATGATCATCATTCTCTTGTGCAGTGTG
  GCCGTCACAGTTCTCTCTGCCATCAATGTGGGGAAGAGCATCATGTTTGCCATGACACCA
  CTCTTGATTGCCACCTCCTCCCTGATGCCTTTTATTGGCTTTCTGCTGGGTTATGTTCTC
  TCTGCTCTCTTCTGCCTCAATGGACGGTGCAGACGCACTGTCAGCATGGAGACTGGATGC
  CAAAATGTCCAACTCTGTTCCACCATCCTCAATGTGGCCTTTCCACCTGAAGTCATTGGA
  CCACTTTTCTTCTTTCCCCTCCTCTACATGATTTTCCAGCTTGGAGAAGGGCTTCTCCTC
  ATTGCCATATTTTGGTGCTATGAGAAATTCAAGACTCCCAAGGATAAAACAAAAATGATC
  TACACAGCTGCCACAACTGAAGAAACAATTCCAGGAGCTCTGGGAAATGGCACCTACAAA
  GGGGAGGACTGCTCCCCTTGCACAGCCTAG
  
  Clone variation with respect to NM_003049.3
  225 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003049 unedited
  CCCCCCGCCGCNACANCCNCCCCTACCCNNNCCNGGGGTTTCGNNATTTGTNATACGACT
  TACTATAGGGCGGCCGCGCATTCGGCACGAGCTCTGTCCTGAGNAGTGATTAAAGAAGGC
  ATCCAGCAAGAACTGCACAAGAAACGGAGTCAGCCGGAGAACAAGGAGTGGTCTTCCACT
  GCCTCACAGGAGGATGGAGGCCCACAACGCGTCTGCCCCATTCAACTTCACCCTGCCACC
  CAACTTTGGCAAGCGCCCCACAGACCTGGCACTGAGCGTCATCCTGGTGTTCATGTTGTT
  CTTCATCATGCTCTCGCTGGGCTGCACCATGGAGTTCAGCAAGATCAAGGCTCACTTATG
  GAAGCCTAAAGGGCTGGCCATCGCCCTGGTGGCACAGTATGGCATCATGCCCCTCACAGC
  CTTTGTGCTGGGCAAGGTCTTCCGGCTGAAGAACATTGAGGCACTGGCCATCTTGGTCTG
  TGGCTGCTCACCTGGAGGGAACCTGTCCAATGTCTTCAGTCTGGCCATGAAGGGGGACAT
  GAACCTCAGCATTGTGATGACCACCTGCTCCACCTTCTGTGCCCTTGGCATGATGCCTCT
  CCTCCTGTACATCTACTCCAGGGGGATCTATGATGGGGACCTGAAGGACAAGGTGCCCTA
  TAAAGGCATCGTGATATCACTGGTCCTGGTTCTCATTCCTTGCACCATAGGGATCGTCCT
  CAAATCCAAACGGCCACAATACATGCGCTATGTCATCAAGGGAAGGATGATCATCATTCT
  CTTGTGCAGTGTGGCCGTCACAGTTCTCTCTGCCATCAAATGTGGGGAAGAGCATCATGT
  TTGCCATGACACCACTCTTGATTGCCACCTCCTCCCTGATGCCTTNTATNGNCTNTCTGC
  CTGGGTAATGTCTCTCTGCTCTCTTCTGCCTCAATGGACGGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003049 unedited
  ACTTGNACCCGGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTTTTAGGTTAGAAC
  TTCTGAAGTTTAATTCTACAGGAGTTGTGAGCATTTATTTCTCTTTTGGGTCACAAAACT
  TGGAATGGGATTTGGGTATTTGAGTAATGGGTTAAACATATATATTGATTAGTTCAGGAA
  TTGAGTGACTTTAAGATGCTTATCAGACACTTTTAGAGATCCCAGCAAGAGGCAGATTCG
  GTTTCTGGTTTCATAGAGTTTACAGTCACTGAACAAGTCTTTAAAATAAGTAGCAAATTC
  TAAGTTGGGGATAGGTGAGGCTTCTTGGGTAGACACCCTGTCTGTGTTCCCGGCCAAGAC
  TTGATGATTCTGATAGATGTACTGGAAATGCTGGAGAAAGACTCANNGCAGACTGGTGTT
  TTTGCTGCTCTCTCTAGTTACCACACTGGCTTTCAGAAATGCTTTGGGACCAGAATCCAN
  GCCACCCAGGGGAGGGCTAGGCTGTGCAAGGGGAGCAGTCCTCCCCTTGGAGGTGCCATT
  TCCCAAAGCTCCTGGAAATTGTTCTTCATTTGGGGCACCTGGGTAGATCATTTTTGTTTT
  ATCCTTGGAATCTGAATTTTTCATACCCCAAATTTTGCATTGGGAAAAGCCTTTTTCAGC
  TGGAAAATCTTTAAAGGAGGGAAAAAAAAAAAGGTCCATGACCTTGGGGGAAAGGCCCTT
  TAGTTGGGGGAAAAAATGGACTTTTTGGTTCCAATTCTATTTGAAAAGGGTTTCCCCCCC
  TTTGGGCCAAAAAACCAAAACTTACCCCCCAAACCCTAAAGGTTTGGGGGGGGGGACAAA
  AAATGTGTTGGTAAAAAAAAGTCTTCCCCTTTTTGGGAAAAAACTCTCCGCCCCCTCCAA
  AAAAAGTAACATCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003049
• Insert Size: 1750 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003049.1, NP_003040.1
• RefSeq Size: 1580 bp
• RefSeq ORF: 1050 bp
• Locus ID: 6554
• Cytogenetics: 14q24.1
• Domains: SBF
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'The protein encoded by this gene belongs to the sodium/bile acid cotransporter family, which are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids; the ileal sodium/bile acid cotransporter with an apical cell localization that absorbs bile acids from the intestinal lumen, bile duct and kidney, and the liver-specific sodium/bile acid cotransporter, represented by this protein, that is found in the basolateral membranes of hepatocytes. Bile acids are the catabolic product of cholesterol metabolism, hence this protein is important for cholesterol homeostasis. [provided by RefSeq, Oct 2011]'